Papers by Kiarash Ghazvini
The role of Chlamydia pneumoniae in asthma has drawn much attention in recent years. In this stud... more The role of Chlamydia pneumoniae in asthma has drawn much attention in recent years. In this study we assessed the prevalence of C. pneumoniae infections in patients with chronic stable and acute exacerbation of asthma and compared it with normal population. Twenty adult patients with chronic stable asthma and 21 patients with acute exacerbations of asthma and 41 matched control
The role of Chlamydia pneumoniae in asthma has drawn much attention in recent years. In this stud... more The role of Chlamydia pneumoniae in asthma has drawn much attention in recent years. In this study we assessed the prevalence of C. pneumoniae infections in patients with chronic stable and acute exacerbation of asthma and compared it with normal population. Twenty adult patients with chronic stable asthma and 21 patients with acute exacerbations of asthma and 41 matched control
Journal of medical microbiology, Jan 19, 2015
This study determined the mechanisms and patterns of antimicrobial resistance among the isolates ... more This study determined the mechanisms and patterns of antimicrobial resistance among the isolates obtained from different wards of a teaching hospital in the city of Mashhad in northeast-Iran. Between January 2012 and the end of June 2012, 36 isolates of Acinetobacter baumannii (A. baumannii) were collected from different wards of Ghaem Hospital. Antimicrobial susceptibility testing and epsilometer-testing (E-test) were performed. The genetic resistance determinants of A, B and D classes of β-lactamases, aminoglycoside modifying enzymes (AMEs), efflux pumps and ISAba1 elements were assessed by PCR. REP-PCR was performed to find the genetic relatedness of the isolates. Colistin was the most effective antibiotic of those tested, where all isolates were susceptible. E-test results revealed high rates of resistance to imipenem, ceftazidime and ciprofloxacin. The majority of isolates (97%) were multidrug-resistant (MDR). OXA-51, OXA-23 and tetB genes were detected in all isolates, but OXA...
Avicenna journal of phytomedicine
In this study, antimicrobial activities of an ethanol extract of Tribulus terrestris aloneand in ... more In this study, antimicrobial activities of an ethanol extract of Tribulus terrestris aloneand in combination with Capsella bursa-pastoris and Glycyrrhiza glabra were examined in vitro against six pathogens namely Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus, Enterococcus faecalis Staphylococcus aureus, and Escherichia coli. Antibacterial activities of the extracts were examined using disc and well diffusion methods and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ethanol extracts were determined against these microorganisms using agar and broth dilution methods. Chlorhexidine was used as positive control. Tribulus terrestris extract exhibited good antibacterial activity against all bacteria. Antibacterial activity of mixed extract was evaluated and exhibited that mixed extract was more effective against all bacteria than any of the cases alone which indicates the synergistic effect between these three extracts (p˂0.0...
Tuberculosis is a well-known infectious disease in human beings and domestic animals since ancien... more Tuberculosis is a well-known infectious disease in human beings and domestic animals since ancient times. Tuberculin skin test as the only indicator of latent infection with Mycobacterium tuberculosis has a low specificity and sensitivity value. This test also cannot distinguish between tuberculosis infection and Mycobacterium bovis BCG vaccination, or exposure to environmental mycobacteria. Recent identification of the RD1 region of M. tuberculosis provides a new opportunity for the development of novel diagnostic tools. The purpose of this study was to clone and express the 10-kda culture filtrate protein (CFP-10) of M. tuberculosis in soluble form to be applicable for diagnostic purposes. To reach this aim, DNA was extracted from M. tuberculosis H37Rv and CFP-10 gene was then amplified by using specific primers. Specificity of PCR products were confirmed, and then were cloned into the pET102/D vector. After sequencing and confirming the insertion of desired PCR product into expre...
Indian Journal of Pathology and Microbiology
Context: Lung cancer is the leading cause of cancer death worldwide. In addition to smoking, a va... more Context: Lung cancer is the leading cause of cancer death worldwide. In addition to smoking, a variety of other contributing factors, including viral infection, have been suggested in tumorigenesis. Epstein Barr virus (EBV), which is linked to various malignancies, seems to be a good candidate. Aims: The aim of this study was to investigate the association of EBV with lung carcinomas. Settings and Design: A total number of 90 formalin fixed paraffin embedded lung tissue samples including 48 cases of lung cancers (18 squamous cell carcinomas [SCCs], 18 adenocarcinomas and 12 small cell carcinomas) and 42 non-tumoral samples (control group), were retrieved from the pathology archive. Materials and Methods: Following deoxyribonucleic acid extraction, polymerase chain reaction (PCR) was performed using an EBV-Eph PCR kit. The positive cases were studied immunohistochemically for the expression of EBV-late membrane protein-1 (EBV-LMP-1) in tumoral tissues. Statistical Analysis Used: The ...
Indian Journal of Pathology and Microbiology
Context: Lung cancer is the leading cause of cancer death worldwide. In addition to smoking, a va... more Context: Lung cancer is the leading cause of cancer death worldwide. In addition to smoking, a variety of other contributing factors, including viral infection, have been suggested in tumorigenesis. Epstein Barr virus (EBV), which is linked to various malignancies, seems to be a good candidate. Aims: The aim of this study was to investigate the association of EBV with lung carcinomas. Settings and Design: A total number of 90 formalin fixed paraffin embedded lung tissue samples including 48 cases of lung cancers (18 squamous cell carcinomas [SCCs], 18 adenocarcinomas and 12 small cell carcinomas) and 42 non-tumoral samples (control group), were retrieved from the pathology archive. Materials and Methods: Following deoxyribonucleic acid extraction, polymerase chain reaction (PCR) was performed using an EBV-Eph PCR kit. The positive cases were studied immunohistochemically for the expression of EBV-late membrane protein-1 (EBV-LMP-1) in tumoral tissues. Statistical Analysis Used: The ...
Jundishapur Journal of Microbiology, 2015
Tuberculosis (TB) is the world's second most common infec... more Tuberculosis (TB) is the world's second most common infectious disease after Human Immunodeficiency Virus Infection/Acquired Immunodeficiency Syndrome (HIV/AID) and the most frequent cause of mortality especially in developing countries. T regulatory (Treg) cells, which have suppressive activity and express forkhead winged-helix family transcriptional repressor p3 (FoxP3), suppress the immune responses against pathogens such as Mycobacterium tuberculosis. There are controversial results regarding the role of FoxP3 expressing cells in the blood of patients with TB. The aim of this study was to evaluate the frequency CD4+ CD25+ Treg cells, and FoxP3 and Cytotoxic T Lymphocyte Antigen 4 (CTLA-4) gene expressions in peripheral blood of patients with tuberculosis and patients with positive tuberculin skin test before and after Peripheral Blood Mononuclear Cells (PBMCs) activation with Purified Protein Derivative (PPD). In this cross-sectional study, Peripheral Mononuclear Cells (PBMCs) were isolated from peripheral blood of 29 patients with newly diagnosed pulmonary TB and 19 patients with positive tuberculin skin test. The PBMCs were activated with PPD for 72 hours. Activated cells were harvested, RNA was extracted and cDNA was synthesized. A real-time Taqman method was designed and optimized for evaluation of Foxp3 gene expression and SYBR Green method was used and optimized for evaluation of CTLA-4 gene expression. A flow cytometry analysis was used to evaluate the frequency of CD4+ CD25+ Foxp3+ regulatory T cells in both groups. There was no significant difference in the frequency of CD4+ CD25+ FoxP3+ regulatory T cells between the two groups. Expression of FoxP3 and CTLA-4 in peripheral blood of patients with newly diagnosed TB was significantly lower than the control group after and before activation with PPD. The expression of FoxP3 and CTLA-4 in PBMCs of patients with newly diagnosed TB was low, which might suggest that Treg cells may be sequestered in the lungs.
Iranian journal of microbiology, 2013
Methicillin Resistant Staphylococcus aureus (MRSA) strains are divided into Community Associated ... more Methicillin Resistant Staphylococcus aureus (MRSA) strains are divided into Community Associated (CA-) and Hospital Associated (HA-) MRSA. These strains vary in antimicrobial resistance and pathogenicity. S. aureus is one of the most common microorganisms in ocular infections. This study was aimed to determine antimicrobial resistance patterns and genetic characteristics of MRSA strains isolated from ocular infections in Iran. Out of 171 S. aureus strains isolated from various clinical samples during September-December 2011 at Mashhad Emam Reza Hospital, 3 were cultured from eye discharge samples. Antimicrobial resistance tests were performed with MIC and disk diffusion methods and also genetic evaluation was done with Staphylococcal Cassette Chromosome mec (SCCmec), Accessory Gene Regulator (agr) and Staphylococcal Protein A (spa) typing, Multi Locus Sequence Typing (MLST) and determination of toxin gene profile. All strains were MRSA and showed resistance to tetracycline, gentamic...
Iranian journal of microbiology, 2013
Methicillin Resistant Staphylococcus aureus (MRSA) strains are divided into Community Associated ... more Methicillin Resistant Staphylococcus aureus (MRSA) strains are divided into Community Associated (CA-) and Hospital Associated (HA-) MRSA. These strains vary in antimicrobial resistance and pathogenicity. S. aureus is one of the most common microorganisms in ocular infections. This study was aimed to determine antimicrobial resistance patterns and genetic characteristics of MRSA strains isolated from ocular infections in Iran. Out of 171 S. aureus strains isolated from various clinical samples during September-December 2011 at Mashhad Emam Reza Hospital, 3 were cultured from eye discharge samples. Antimicrobial resistance tests were performed with MIC and disk diffusion methods and also genetic evaluation was done with Staphylococcal Cassette Chromosome mec (SCCmec), Accessory Gene Regulator (agr) and Staphylococcal Protein A (spa) typing, Multi Locus Sequence Typing (MLST) and determination of toxin gene profile. All strains were MRSA and showed resistance to tetracycline, gentamic...
Avicenna journal of phytomedicine, 2012
Oral infections and dental caries are still considered as serious public health problems and infl... more Oral infections and dental caries are still considered as serious public health problems and inflict a costly burden to health care services around the world and especially in developing countries. In the present study, we evaluated the antibacterial activity of Glycyrrhiza glabra (G. glabra) against oral pathogens by diffusion methods and determined the minimum inhibitory concentration (MIC) by both broth and Agar dilution methods and minimum bactericidal concentration (MBC) by broth dilution methods. In this study, G. glabra extract showed good antibacterial activity against six bacteria. No strain in this study showed resistance against this extract. G. glabrais suggested as an appropriate candidate to help us in order to control dental caries and endodontic infections.
Dental research journal, 2012
The aim of this study was to evaluate the antimicrobial activity of Mineral Trioxide Aggregate (M... more The aim of this study was to evaluate the antimicrobial activity of Mineral Trioxide Aggregate (MTA) and Calcium Enrich Mixture) CEM (mixed with different concentrations of chlorhexidine (CHX). Cements used in this in vitro study included Gray proRoot MTA and CEM with the microorganisms being entrococcus faecalis, streptococcus muntas, Candida albicans, Actinomyces, Escherichia coli, and a mixture of these microorganisms. CHX was used in the form of liquid at 0.2%, 2%, and 0.12% concentrations. Contact dilution and colony count method was used to evaluate the antibacterial activity of these cements. After 0, 24, 48, 72, and 96-hour intervals, we cultured the samples on blood agar medium. Colonies were counted after incubation at 37°. Data were statistically analyzed by a Kruskal-Wallis test to compare the antimicrobial activity of MTA and CEM. All concentrations of CHX were mixed with MTA and the CEM had antibacterial activities on all microorganisms' strains except for the Ente...
Dental research journal, 2012
The aim of this study was to evaluate the antimicrobial activity of Mineral Trioxide Aggregate (M... more The aim of this study was to evaluate the antimicrobial activity of Mineral Trioxide Aggregate (MTA) and Calcium Enrich Mixture) CEM (mixed with different concentrations of chlorhexidine (CHX). Cements used in this in vitro study included Gray proRoot MTA and CEM with the microorganisms being entrococcus faecalis, streptococcus muntas, Candida albicans, Actinomyces, Escherichia coli, and a mixture of these microorganisms. CHX was used in the form of liquid at 0.2%, 2%, and 0.12% concentrations. Contact dilution and colony count method was used to evaluate the antibacterial activity of these cements. After 0, 24, 48, 72, and 96-hour intervals, we cultured the samples on blood agar medium. Colonies were counted after incubation at 37°. Data were statistically analyzed by a Kruskal-Wallis test to compare the antimicrobial activity of MTA and CEM. All concentrations of CHX were mixed with MTA and the CEM had antibacterial activities on all microorganisms' strains except for the Ente...
Iranian journal of basic medical sciences, 2012
Human papillomavirus (HPV) infections are related to the genesis of various benign lesions and so... more Human papillomavirus (HPV) infections are related to the genesis of various benign lesions and some malignant tumors, but no clear relationship has been identified so far between the subtypes of HPV and skin tag. The present case-control study was designed to detect the existence of low risk and high risk HPV types in lesions of 50 patients with skin tag (case group) and normal skin around the melanocytic nevus of 30 patients (control group), using PCR. All of the samples were negative for HPV subtypes, except two samples in control group which were positive for high risk HPV. There was no significant relationship between the HPV subtypes and skin tag. There is no association between skin tag and low risk and high risk human papillomaviruses.
Iranian journal of basic medical sciences, 2012
Human papillomavirus (HPV) infections are related to the genesis of various benign lesions and so... more Human papillomavirus (HPV) infections are related to the genesis of various benign lesions and some malignant tumors, but no clear relationship has been identified so far between the subtypes of HPV and skin tag. The present case-control study was designed to detect the existence of low risk and high risk HPV types in lesions of 50 patients with skin tag (case group) and normal skin around the melanocytic nevus of 30 patients (control group), using PCR. All of the samples were negative for HPV subtypes, except two samples in control group which were positive for high risk HPV. There was no significant relationship between the HPV subtypes and skin tag. There is no association between skin tag and low risk and high risk human papillomaviruses.
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Papers by Kiarash Ghazvini