(Q53616216)

16 May 2018

title

RAG and HMGB1 proteins: purification and biochemical analysis of recombination signal complexes. (English)

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16 May 2018

author name string

Serge Bergeron

series ordinal

1

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16 May 2018

Dirk K Anderson

series ordinal

2

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16 May 2018

Patrick C Swanson

series ordinal

3

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16 May 2018

language of work or name

English

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publication date

1 January 2006

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16 May 2018

published in

Methods in Enzymology

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16 May 2018

volume

408

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16 May 2018

page(s)

511-528

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16 May 2018

Deletion of the RAG2 C terminus leads to impaired lymphoid development in mice

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The mechanism and regulation of chromosomal V(D)J recombination

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Both high mobility group (HMG)-boxes and the acidic tail of HMGB1 regulate recombination-activating gene (RAG)-mediated recombination signal synapsis and cleavage in vitro

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7 January 2021

The lack of chromosomal protein Hmg1 does not disrupt cell growth but causes lethal hypoglycaemia in newborn mice

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A short peptide at the C terminus is responsible for the nuclear localization of RAG2.

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Analysis of regions of RAG-2 important for V(D)J recombination

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Impaired V(D)J recombination and lymphocyte development in core RAG1-expressing mice.

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High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

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7 January 2021

The C-terminal portion of RAG2 protects against transposition in vitro

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7 January 2021

The RAG proteins and V(D)J recombination: complexes, ends, and transposition

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V(D)J recombination: RAG proteins, repair factors, and regulation

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Assembly of a 12/23 Paired Signal Complex: A Critical Control Point in V(D)J Recombination

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7 January 2021

Cell cycle-dependent accumulation in vivo of transposition-competent complexes between recombination signal ends and full-length RAG proteins

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Ubiquitylation of RAG-2 by Skp2-SCF links destruction of the V(D)J recombinase to the cell cycle

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Autoubiquitylation of the V(D)J recombinase protein RAG1

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7 January 2021

Regions of RAG1 protein critical for V(D)J recombination

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Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytes

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7 January 2021

A conserved degradation signal regulates RAG-2 accumulation during cell division and links V(D)J recombination to the cell cycle

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7 January 2021

The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development

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Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination

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7 January 2021

Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

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7 January 2021

RAG2 is down-regulated by cytoplasmic sequestration and ubiquitin-dependent degradation

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7 January 2021

Assembly of the RAG1/RAG2 synaptic complex

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7 January 2021

RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination

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7 January 2021

Overlapping signals for protein degradation and nuclear localization define a role for intrinsic RAG-2 nuclear uptake in dividing cells

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7 January 2021

Definition of a core region of RAG-2 that is functional in V(D)J recombination

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7 January 2021

Expression and V(D)J recombination activity of mutated RAG-1 proteins

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7 January 2021

V(D)J recombination: modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins

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7 January 2021

The V(D)J recombination activating gene, RAG-1.

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7 January 2021

Increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs.

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7 January 2021

Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination

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7 January 2021

The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination

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7 January 2021

Roles of the "dispensable" portions of RAG-1 and RAG-2 in V(D)J recombination

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7 January 2021

The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination

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7 January 2021

Fine structure and activity of discrete RAG-HMG complexes on V(D)J recombination signals

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7 January 2021

A RAG-1/RAG-2 tetramer supports 12/23-regulated synapsis, cleavage, and transposition of V(D)J recombination signals

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7 January 2021

The bounty of RAGs: recombination signal complexes and reaction outcomes

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7 January 2021

Full-length RAG-2, and Not Full-length RAG-1, Specifically Suppresses RAG-mediated Transposition but Not Hybrid Joint Formation or Disintegration

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7 January 2021

Increased frequency of aberrant V(D)J recombination products in core RAG-expressing mice

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7 January 2021

HMG1 and 2, and related 'architectural' DNA-binding proteins

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7 January 2021

Regulation of RAG1/RAG2-mediated transposition by GTP and the C-terminal region of RAG2

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7 January 2021

Stimulation of V(D)J cleavage by high mobility group proteins

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7 January 2021

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7 January 2021

The RAG1 N-terminal domain is an E3 ubiquitin ligase

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7 January 2021

10.1016/S0076-6879(06)08032-3

Identifiers

DOI

1 reference

16 May 2018

1 reference

16 May 2018