Papers by Pushpesh Mishra
517-521The present study was designed to investigate anti-ulcerogenic property of ethanolic extra... more 517-521The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of Desmodium gangeticum (DG) against cold restraint (CRU, 2 hr cold restraint stress), aspirin (ASP, 150 mg/kg orally), alcohol (AL, absolute alcohol 1m1/200gm) and pyloric ligation (PL, 4 hr pylorus ligation) induced gastric ulcer models in Sprague Dawley rats, and histamine (HST, 0.25 mg/kg) induced duodenal ulcer in guinea pigs. We found that DG at a dose of 200mg/kg, (orally), markedly decreased the incidence of ulcers in all the above models. DG showed significant protection against CRU (68.37%), AL (88.87%), ASP (38.2%), PL (40.63%) and HST (63.15%) induced ulcer models, whereas standard drug omeprazole (OMZ) showed protection index of 83.86, 56.35, 70.31 and 84.21%, respectively in CRU, ASP, PL and HST models. Sucralfate as standard drug showed 92.64% protection in AL model. DG significantly reduced acid secretion 41.61 %, whereas OMZ produced 43.13% reduction. Treatment with D...
To determine the anticonvulsant activity of leaf extract of Borassus flabellifer Linn. in Wistar ... more To determine the anticonvulsant activity of leaf extract of Borassus flabellifer Linn. in Wistar albino rats and in order to verify the traditional use of the plant in the treatment of epilepsy. The Maximal Electro Shock Seizure (MES) and Pentylenetetrazole (PTZ) were used for assessing the anticonvulsant activity of alcoholic and aqueous leaf extracts of Borassus flabellifer on wistar albino rats. The acute toxicity study was conducted as per guidelines set by OECD, and no adverse effects or mortality were detected up to 4g/kg,p.o.,based on the results obtained from the study the dose for anticonvulsant activity was fixed to be 100mg,200mg,400mg/kg b.w.,for dose dependent study. The alcoholic extract (400mg) of Borassus flabellifer had shown potent anticonvulsant activity than other extracts when compare to control group and the phenytoin and diazepam treated animals shown 100 percent protection against convulsant.The result from the study indicate that the 400mg of alcoholic leaf ...
Indian journal of experimental biology, 2005
The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of D... more The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of Desmodium gangeticum (DG) against cold restraint (CRU, 2 hr cold restraint stress), aspirin (ASP, 150 mg/kg orally), alcohol (AL, absolute alcohol 1 ml/200gm) and pyloric ligation (PL, 4 hr pylorus ligation) induced gastric ulcer models in Sprague Dawley rats, and histamine (HST, 0.25 mg/kg) induced duodenal ulcer in guinea pigs. We found that DG at a dose of 200mg/kg, (orally), markedly decreased the incidence of ulcers in all the above models. DG showed significant protection against CRU (68.37%), AL (88.87%), ASP (38.2%), PL (40.63%) and HST (63.15%) induced ulcer models, whereas standard drug omeprazole (OMZ) showed protection index of 83.86, 56.35, 70.31 and 84.21%, respectively in CRU, ASP, PL and HST models. Sucralfate as standard drug showed 92.64% protection in AL model. DG significantly reduced acid secretion 41.61%, whereas OMZ produced 43.13% reduction. Treatment with DG showe...
The ethanolic extract of the whole plant of Anisoch ilus carnosus (Lamiaceae) was evaluated for h... more The ethanolic extract of the whole plant of Anisoch ilus carnosus (Lamiaceae) was evaluated for hepatop ro ective activity in wistar rats with liver damage induced b y paracetamol. The extract of an oral dose of (200 mg/kg, bw and 400 mg/kg, bw p.o.) exhibited a significant protect ive effect against paracetamol induced hepatotoxici ty by lowering the serum levels of aspartate aminotransferase (SGO T), alanine aminotransferase (SGPT), total serum bi liru in, and malondialdehyde equivalent, an index of lipid p eroxidation of the liver. The activity of the ethan olic extract of Anisochilus carnosus whole plant was comparable to the standard drug, silymarin (100 mg/kg, p.o.), and the histopathological studies supports the above findin g. Results indicate that Anisochilus carnosus posse sses significant hepatoprotective effect on paracetmol induced hepatotoxicity in mice.
Hepatotoxicity is one of the major side effects of anti-tubercular drug like Isoniazid. The aim o... more Hepatotoxicity is one of the major side effects of anti-tubercular drug like Isoniazid. The aim of Study was designed to evaluate the hepatoprotective activity of Carica Papaya Latex at the dose 400mg/k.g. and Ficus Bengalensis Latex at the dose 300mg/k.g. per oral induced by isoniazid at the dose 50mg/k.g. per oral. Group-I serve as a normal control received Saline 1ml/Kg. Group-II were received Isoniazid (50mg/kg.)-per oral. Group-III were received Carica Papaya Latex 400mg/Kg. b.w./oral 30 min, prior to Isoniazid (50mg/kg .). Group-IV were received Ficus Bengalensis Latex 300mg/Kg./oral 30 min, prior to Isoniazid (50mg/kg .). Group-V were received Silymarin suspended in 1% of CMC 100mg/Kg./oral 30 min, prior to Isoniazid (50mg/kg .) in sterile saline once daily for 30 days. Animals were sacrificed on 30 days. Blood were collected by Cardiac puncture. Hepatoprotective activity was observed by using biochemical parameters such as Serum-Glutamate Pyruvate Transaminase and Serum-Glutamate Oxalate Transaminase (SGPT and SGOT), total protein, Bilirubin, Alkaline Phosphate (ALP). Carica papaya Latex and Ficus Bengalensis Latex formed major hepatoprotective effect by decreasing the activity of serum enzyme as SGPT, SGOT, Bilirubin, ALP, and prominent total protein level. Both plant latex were observed to prevent the toxic effect of Isoniazid on above serum parameters.
To determine the anticonvulsant activity of leaf extract of Bauhinia purpurea Linn. in Wistar alb... more To determine the anticonvulsant activity of leaf extract of Bauhinia purpurea Linn. in Wistar albino rats and in order to verify the traditional use of the plant in the treatment of epilepsy. The Maximal Electro Shock Seizure (MES) and Pentylenetetrazole (PTZ) were used for assessing the anticonvulsant activity of alcoholic and aqueous leaf extracts of Bauhinia purpurea on wistar albino rats. The acute toxicity study was conducted as per guidelines set by OECD, and no adverse effects or mortality were detected up to 4g/kg,p.o., based on the results obtained from the study the dose for anticonvulsant activity was fixed to be 100mg,200mg,400mg/kg b.w., for dose dependent study. The alcoholic extract (400mg) of Bauhinia purpurea had shown potent anticonvulsant activity than other extracts when compare to control group and the phenytoin and diazepam treated animals shown 100 percent protection against convulsant. The result from the study indicate that the 400mg of alcoholic leaf extrac...
Indian Journal of Experimental Biology, Jul 1, 2005
The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of D... more The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of Desmodium gangeticum (DG) against cold restraint (CRU, 2 hr cold restraint stress), aspirin (ASP, 150 mg/kg orally), alcohol (AL, absolute alcohol 1 ml/200gm) and pyloric ligation (PL, 4 hr pylorus ligation) induced gastric ulcer models in Sprague Dawley rats, and histamine (HST, 0.25 mg/kg) induced duodenal ulcer in guinea pigs. We found that DG at a dose of 200mg/kg, (orally), markedly decreased the incidence of ulcers in all the above models. DG showed significant protection against CRU (68.37%), AL (88.87%), ASP (38.2%), PL (40.63%) and HST (63.15%) induced ulcer models, whereas standard drug omeprazole (OMZ) showed protection index of 83.86, 56.35, 70.31 and 84.21%, respectively in CRU, ASP, PL and HST models. Sucralfate as standard drug showed 92.64% protection in AL model. DG significantly reduced acid secretion 41.61%, whereas OMZ produced 43.13% reduction. Treatment with DG showed increase in mucin secretion by 56.17%, whereas OMZ showed 12.45% increase. Anti-ulcer effect of DG may be due to its cytoprotective effect along with antisecretory activity and could act as a potent therapeutic agent against peptic ulcer disease.
Journal of Medicinal Plants Research, 2012
Microwave assisted a series of novel curcumin analogues that were synthesized by altering β-diket... more Microwave assisted a series of novel curcumin analogues that were synthesized by altering β-diketone system with olifinic bond (4 to 12) and evaluated their anticancer activity against KB-oral and mouth, CaCo2-colon, MCF-7-breast, WRL-68 liver, HepG-2-liver human cancer cell lines. The naturally occurring curcuminods (A, B and C) showed excellent IC 50 ranging from 0.0006 to 6.0 µg/ml. Analogues 5, 7, 10 and 12 showed very good anticancer activity with IC 50 value 0.003, 0.0012, 0.33 and 0.067 µg/ml, respectively as compare to curcumin and taxol with IC 50 0.044 and 0.001 µg/ml, respectively.
Indian journal of experimental biology
The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of D... more The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of Desmodium gangeticum (DG) against cold restraint (CRU, 2 hr cold restraint stress), aspirin (ASP, 150 mg/kg orally), alcohol (AL, absolute alcohol 1 ml/200gm) and pyloric ligation (PL, 4 hr pylorus ligation) induced gastric ulcer models in Sprague Dawley rats, and histamine (HST, 0.25 mg/kg) induced duodenal ulcer in guinea pigs. We found that DG at a dose of 200mg/kg, (orally), markedly decreased the incidence of ulcers in all the above models. DG showed significant protection against CRU (68.37%), AL (88.87%), ASP (38.2%), PL (40.63%) and HST (63.15%) induced ulcer models, whereas standard drug omeprazole (OMZ) showed protection index of 83.86, 56.35, 70.31 and 84.21%, respectively in CRU, ASP, PL and HST models. Sucralfate as standard drug showed 92.64% protection in AL model. DG significantly reduced acid secretion 41.61%, whereas OMZ produced 43.13% reduction. Treatment with DG showe...
European Journal of Medicinal Chemistry
Ficus racemosa belongs to the family of Moraceae and is commonly known as 'Gular' in nort... more Ficus racemosa belongs to the family of Moraceae and is commonly known as 'Gular' in north India. Bio-activity guided isolation work on the fruits of F. racemosa resulted in the identification of antidiabetic active principle, a-amyrin acetate 7. Compound 7 lowered the blood glucose levels by 18.4 and 17.0% at 5 and 24 h, respectively, in sucrose challenged streptozotocin induced diabetic rat (STZ-S) model at the dose of 100 mg/kg body weight. Fifteen novel derivatives viz, 9–21, 24, 25 of a-amyrin 8 were prepared and their antihyperglycemic activity profile was assessed. The p-chlorobenzoic acid derivative 9 and nicotinic acid derivative 14 showed potent antihyperglycemic activity at 100 mg/kg body weight. Ó 2008 Elsevier Masson SAS. All rights reserved.
Indian journal of experimental biology, 2005
The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of D... more The present study was designed to investigate anti-ulcerogenic property of ethanolic extract of Desmodium gangeticum (DG) against cold restraint (CRU, 2 hr cold restraint stress), aspirin (ASP, 150 mg/kg orally), alcohol (AL, absolute alcohol 1 ml/200gm) and pyloric ligation (PL, 4 hr pylorus ligation) induced gastric ulcer models in Sprague Dawley rats, and histamine (HST, 0.25 mg/kg) induced duodenal ulcer in guinea pigs. We found that DG at a dose of 200mg/kg, (orally), markedly decreased the incidence of ulcers in all the above models. DG showed significant protection against CRU (68.37%), AL (88.87%), ASP (38.2%), PL (40.63%) and HST (63.15%) induced ulcer models, whereas standard drug omeprazole (OMZ) showed protection index of 83.86, 56.35, 70.31 and 84.21%, respectively in CRU, ASP, PL and HST models. Sucralfate as standard drug showed 92.64% protection in AL model. DG significantly reduced acid secretion 41.61%, whereas OMZ produced 43.13% reduction. Treatment with DG showe...
Phytochemistry, 2004
Five new flavonoid C-glucosides, 6-hydroxy-2-(4-hydroxybenzyl)-benzofuran-7 and 1,2-bis(2,4-dihyd... more Five new flavonoid C-glucosides, 6-hydroxy-2-(4-hydroxybenzyl)-benzofuran-7 and 1,2-bis(2,4-dihydroxy,3-Cglucopyranosyl)-ethanedione (6) and two known compounds C-b-d-glucopyranosyl-2,6-dihydroxyl benzene (7) and sesquiterpene (8), were isolated from an aqueous extract of the heartwood of Pterocarpus marsupium. The structure has been established using spectroscopic data. #
Phytochemistry, 2004
Four new clerodane furano diterpene glucosides (amritosides A, B, C and D) were isolated as their... more Four new clerodane furano diterpene glucosides (amritosides A, B, C and D) were isolated as their acetates from Tinospora cordifolia stems. The structures of these compounds were established on the basis of spectroscopic studies.
Medicinal Research Reviews, 2009
This review chronicles the exploration of the curcumin in terms of development of analogues for t... more This review chronicles the exploration of the curcumin in terms of development of analogues for the anticancer activity over the last century.
Journal of Ethnopharmacology, 2005
Allophylus serratus is known to possess various therapeutic properties. We evaluated the anti-ulc... more Allophylus serratus is known to possess various therapeutic properties. We evaluated the anti-ulcerogenic property of crude ethanolic extract of Allophylus serratus (AS) in different ulcer models in Sprague-Dawley rats. The extract at 400 mg/kg body weight, once daily, orally has a significant effect in cold restraint (CRU, 2 h cold restraint stress), aspirin (ASA, 150 mg/kg body weight, orally), alcohol (AL, 1 ml/200 gm of absolute alcohol) and pyloric ligation (PL, 4h ligation) induced gastric ulcer models as it showed protection index of 71.28, 62.50, 90.84 and 64.29% protection, respectively whereas, standard drug omeprazole (OMZ, 10mg/kg body weight) has shown protection index of 85.70, 74.99 and 74.99 in CRU, ASA and PL model respectively. Sucralfate (SUC, 500 mg/kg body weight) as a standard drug in AL model has 93.20% protection. Furthermore, AS has significantly decreased the free acidity (72.41%), total acidity (47.97%) and peptic activity (24.59%), respectively as well as has significantly increased the mucus secretion (29.41%). Conclusively the ulcer protective effect of AS may be due to its anti-secretory along with cytoprotective mechanism.
Journal of Ethnopharmacology, 2005
Crude ethanolic extract of Indian medicinal plant, Desmodium gangeticum (A001) and its three frac... more Crude ethanolic extract of Indian medicinal plant, Desmodium gangeticum (A001) and its three fractions-hexane (F002), n-butanol (F003) and aqueous (F004) were evaluated chemoprophylactically and chemotherapeutically against experimental visceral leishmaniasis in hamsters. Ethanolic extract showed 41.2+/-5.3% inhibition of parasite multiplication when administered at a dose of 250 mg/kgx2 on day -7 and +7 of Leishmania donovani challenge. Its n-butanol fraction exhibited better efficacy than the ethanolic extract to the tune of 66.7+/-6.1% inhibition when administered at similar dose schedule. But the other two fractions failed to exert any action prophylactically. F003 also imparted significant (P&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) non-specific resistance to peritoneal macrophages against Leishmania infection. F003 also showed moderate antileishmanial activity when tested against established infection of Leishmania donovani in hamsters but the rest three fractions failed to show any significant inhibition of parasite multiplication. These findings revealed that this plant has potential prophylactic and therapeutic efficacy against Leishmania infection and warrants detailed investigations on its possible immunopotentiatory actions.
European Journal of Medicinal Chemistry, 2009
Ficus racemosa belongs to the family of Moraceae and is commonly known as &amp;amp;amp;amp;#3... more Ficus racemosa belongs to the family of Moraceae and is commonly known as &amp;amp;amp;amp;#39;Gular&amp;amp;amp;amp;#39; in north India. Bio-activity guided isolation work on the fruits of F. racemosa resulted in the identification of antidiabetic active principle, alpha-amyrin acetate 7. Compound 7 lowered the blood glucose levels by 18.4 and 17.0% at 5 and 24 h, respectively, in sucrose challenged streptozotocin induced diabetic rat (STZ-S) model at the dose of 100 mg/kg body weight. Fifteen novel derivatives viz, 9-21, 24, 25 of alpha-amyrin 8 were prepared and their antihyperglycemic activity profile was assessed. The p-chlorobenzoic acid derivative 9 and nicotinic acid derivative 14 showed potent antihyperglycemic activity at 100 mg/kg body weight.
Bioorganic & Medicinal Chemistry Letters, 2005
Nineteen compounds of various classes, such as flavonoid glycosides, pterocarpanoids, lipids, gly... more Nineteen compounds of various classes, such as flavonoid glycosides, pterocarpanoids, lipids, glycolipids, and alkaloids, were isolated and identified from the Desmodium gangeticum whole plant. Aminoglucosyl glycerolipid is reported here for the first time. Its structure has been elucidated by spectroscopic and degradation studies. This novel compound exhibited in vitro antileishmanial and immunomodulatory activities, as it enhanced nitric oxide (NO) production and provided resistance against infection established in peritoneal macrophages by the protozoan parasite Leishmania donovani. Another known compound, glycosphingolipid (cerebroside) (7) was found to possess significant in vitro antileishmanial and immunomodulatory activities against the same parasite. Other compounds were found to be inactive.
Bioorganic & Medicinal Chemistry Letters, 2006
PTPases are considered to be involved in the etiology of diabetes mellitus and neural diseases, s... more PTPases are considered to be involved in the etiology of diabetes mellitus and neural diseases, such as Alzheimer&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s disease and Parkinson&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s disease. Therefore, PTPase inhibitors should be useful tools to study the role of PTPases in these diseases and other biological phenomena, and which can be developed into chemotherapeutic agents. In the present study, we have synthesized novel benzofuran isoxazolines 13-21 via 1,3-dipolar cycloaddition reaction using karanjin (1) and kanjone (2), isolated from Pongamia pinnata fruits. All the synthesized compounds were evaluated against PTPase enzyme. Compounds 19 and 20 displayed significant inhibitory activity with IC50 values 76 and 81 microM, respectively.
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Papers by Pushpesh Mishra