Papers by Ravindra Babu Potti
Journal of Pharmacy Research, Nov 1, 2017
Background and Aim: The vascular endothelium is directly involved in peripheral vascular disease,... more Background and Aim: The vascular endothelium is directly involved in peripheral vascular disease, stroke, heart disease, diabetes, insulin resistance, chronic kidney failure, tumor growth, metastasis, venous thrombosis, and severe viral infectious diseases. Dysfunction of the vascular endothelium is thus a hallmark of human diseases. The present treatment packages available are unable to control/manage the progress of diseases mentioned above. Method: Jatamansi liquid 10 ml contains 500 mg of extract equivalent to 2.5 g raw herb given twice a day on open-label study to 10 subjects suffering with diabetes and hypertension for 15 days, and observation of augmentation pressure (AP) and augmentation index (AIx) done before and after treatment with SPHYGMOCOR machine, which show the vascular resistance and nitric oxide (NO)dependent vascular relaxation at Nizams Institute of Medical Sciences, Hyderabad. The method was performed by using SPHYGMOCOR instrument. Results: The results are encouraging in the reduction of blood pressure, improvement of NO availability, reduction of AP, and AIx shows a considerable reduction in vascular resistance indicating an improvement of endothelial function. Conclusion: To sum up the study, Jatamansi extract showed improvement of NO availability apart from the reduction of blood pressure and reduction of vascular resistance by improving endothelial function, and being a small study, future investigation of Jatamansi extract may aid in better understanding the molecular mechanism associated with the observed protection..
World Journal of Biology Pharmacy and Health Sciences
During one decade, an increasing percentage of world population has to be attributed to an array ... more During one decade, an increasing percentage of world population has to be attributed to an array of environmental, health and lifestyle factors. The potential effect of extremely low frequency electromagnetic fields (ELF-EMFs) emitted from electrical and various electrical devices in the occupational environment and light at night (LAN) to elicit biological response are a major concern for the public and scientists. Controversial genetic and oxidative stress effects have been reported by these polluted environments. Hence this study was undertaken with a purpose to evaluate melatonin hormone and oxidative stress markers in exposure workers along with cumulative effects of smoking and drinking. The study subjects (n=342) included night shift workers for more than 2 years with age, gender, socioeconomic status matched non-exposure controls (n=150). Self-reported symptoms were sought using questionnaire. Plasma Melatonin was evaluated using radioimmuno assay. Oxidative stress was estim...
Zenodo (CERN European Organization for Nuclear Research), Dec 30, 2022
During one decade, an increasing percentage of world population has to be attributed to an array ... more During one decade, an increasing percentage of world population has to be attributed to an array of environmental, health and lifestyle factors. The potential effect of extremely low frequency electromagnetic fields (ELF-EMFs) emitted from electrical and various electrical devices in the occupational environment and light at night (LAN) to elicit biological response are a major concern for the public and scientists. Controversial genetic and oxidative stress effects have been reported by these polluted environments. Hence this study was undertaken with a purpose to evaluate melatonin hormone and oxidative stress markers in exposure workers along with cumulative effects of smoking and drinking. The study subjects (n=342) included night shift workers for more than 2 years with age, gender, socioeconomic status matched non-exposure controls (n=150). Self-reported symptoms were sought using questionnaire. Plasma Melatonin was evaluated using radioimmuno assay. Oxidative stress was estimated by measuring plasma malondialdehyde (MDA), serum nitric oxide (NO) and erythrocyte catalase levels. When the data of exposed subjects was analyzed, a significant decrease in melatonin levels was observed. Plasma MDA levels demonstrated a significant increase in one subgroup. The serum NO levels and erythrocyte catalase levels shows a significant increase. The cumulative effect of a chemical agent or environmental pollutant could be a possible contributor in enhancing the adverse effect of extremely low frequency electromagnetic fields. Certain caution is necessary.
Journal of Pure and Applied Microbiology, Mar 31, 2017
The objective of this research is to evaluate alternative source for collagen. The common source ... more The objective of this research is to evaluate alternative source for collagen. The common source of collagen is from bovine and pig. There is need to find an alternative source of collagen due to its demand. Broiler Chicken feet waste is one of the alternative sources because of its abundant collagen protein content. On an average each chicken shop generates minimum of 30-40 kg waste every day in Hyderabad. Collagen is a fibrillar protein composing different forms of the connective tissue: bone, cartilage, tendon and skin. Collagen is the major component of the extracellular matrix, and more than 27 genetic isoforms have been identified. Collagens type I, II and III are the most abundant and well investigated for biomedical applications. Chicken feet, rather than being transformed into meal for animal feed, a large quantity of chicken feet could be used to produce collagen, which is valued for its unique functional properties. The purpose of this research project was to extract and characterize collagen from chicken feet. Biochemical composition of chicken feet collagen such as moisture, protein, fat and ash content was 5.85, 29.11, 35.43 and 28.60%, respectively.Acid-solubilized collagen (ASC) was isolated from the chicken feet. Molecular weight of ASC was analysed and confirmed by SDS-PAGE. Secondary structure of ASC was confirmed by FT-IR spectrum.Chicken feet thus appear to be a good alternative source of high-quality collagen.
International Journal of Research in Advent Technology, 2020
Commercial herbal products are available were either contaminated or substituted with other plant... more Commercial herbal products are available were either contaminated or substituted with other plant species with similar morphology which are not catalogued on the labels. Adulteration is a serious health threat to the consumer safety. Thus, the genetic level discrimination protocols could be safer to identify the exact and specific herbal products in the market. DNA bar-coding is safe, integrity and authenticated methodologies for species identification and taxon discrimination. The present study discriminated particular herbal plants such as N.jatamansi and S.vaginatum using the DNA bar-coding technology with ITS2 primers and authenticated the originality of the herbal plants.
International Journal of Research in Pharmaceutical Sciences, 2017
Continued oxidative stress can lead to chronic inflammation, which in turn could mediate most chr... more Continued oxidative stress can lead to chronic inflammation, which in turn could mediate most chronic diseases including cancer, diabetes, and cardiovascular, neurological and pulmonary diseases. Oxidation should be arrested in the cells as it leads to chain reactions followed by cell damage. Inflammation induces various chemicals due to which redness and warmth appear at the site of inflammation. Current treatment packages available in the market which may be of allopathic or nutritional are unable to control the mortality of cancer. On the contrary allopathic leads to side effects and increasing the risk of the patient, so there is an immense need for other treatment cum management packages are to be explored in particular from medicinal plants. Jatamansi (Nardostachys Jatamansi) appears to be one of the prospective herbal single drugs which can address oxidative stress and inflammatory situations. Jatmansi showed encouraging anti-inflammatory activity and antioxidant activity, th...
Brevibacterium linens is a normal flora present in the whey of curd, which is a rich source of L ... more Brevibacterium linens is a normal flora present in the whey of curd, which is a rich source of L -Methionine �≥ -lyase (MGL). This enzyme can be utilized as a therapeutic agent in treating cancer. Brevibacterium linens is a gram positive bacterium and it was identified at the molecular level by ribotyping 16s rDNA along with the biochemical characterization, and finally concluded by BLAST analysis by constructing a phylogenetic tree. The enzyme methioninase was purified by precipitating with Ammonium sulphate, dialysis, Anion exchange chromatography and. The purity of methiona se checked by SDS-Polyacrylamide gel electrophoresis and the band corresponding to t he molecular mass of the native enzyme was estimated to be approximately 170 kDa. The activity of the crude enzyme was determined by the production of a -keto acids. The enz yme was stable at pH ranging from 6.0 to 8.0 for 24 h. Freezing and thawing the enzyme solution resulted in a loss of over 60% of the enzyme activity, and...
Informatics in Medicine Unlocked, 2018
Costus igneus, also known as Spiral flag, belongs to the Costaceae family. Recently, studies have... more Costus igneus, also known as Spiral flag, belongs to the Costaceae family. Recently, studies have emphasized the anti-diabetic potential of this plant. Literature studies show that it possess hypo-lipidemic, diuretic, anti-oxidant, anti-microbial, anti-cancerous properties. Phytochemical investigations reveal the presence of carbohydrates, proteins, triterpenoids, alkaloids, tannins, saponins, flavonoids, and steroids. In the present study, thin layer chromatography and high performance liquid chromatography profiling was performed for quercetin. The leaf showed the presence of quercetin with Rf value of 0.56 as compared with the standard Rf value. HPLC chromatograph of Standard and Leaf Extract exhibited peaks at 4.263 minutes and 4.019 minutes. Further quantitative analysis was conducted to determine the total flavonoid content of the leaf extract. It is a naturally occurring bioflavonoid, and an inhibitor of insulin receptor tyrosine kinase-catalyzed phosphorylation of exogenous substrate. Docking studies were performed to determine the interaction of polyphenols with Insulin Receptor Tyrosine Kinase, meant to be a target for diabetes. Insulin receptor kinase (PDB Id:1IR3) was selected as the target molecule. Flavonoids such as quercetin, kaempferol, epicatechin, roseoside, and the standard anti-diabetic drug pioglitazone were selected as ligand molecules. The docked complex was visualized in the Maestro Visualizer of the Schrodinger suite. All of the compounds formed hydrogen bonds with the residues of the receptor. Quercetin showed the best binding energy, with-7.28 kcal/mol indicating improved interactions via hydrogen bonds with the active site residues. Computational studies revealed that quercetin and kaempferol have the best binding energies, as compared to the standard drug pioglitazone (-6.26 kcal/mol), which activates insulin receptor tyrosine kinase. From the present study, we conclude that quercetin can be useful as a herbal therapeutic agent for diabetes.
Journal of Young Pharmacists, 2015
Background: Silencing of a specific mRNA using double stranded RNA oligo nucleotides represents n... more Background: Silencing of a specific mRNA using double stranded RNA oligo nucleotides represents novel technologies for suppressing a specific gene product. Small interfering RNA (siRNA) are 21 nucleotides long, double stranded RNA fragments that interferes with the expression of specific genes with complementary nucleotide sequences. siRNA functions by breaking down mRNA after transcription, resulting in no translation. HER/ErbB is a family of receptors that has HER1, HER2, HER3 and HER4 as its members. Certain mutations in these receptors leads to its over expression. Amplification or over expression of these receptors has shown to play an important role in the development and progression of certain aggressive types of breast cancer, colon and gastric cancers. The SNPs in Her1 receptor were observed at first position, in HER2 at eighth position, in HER3 at first position and in HER4 at third position. Methods: siDirect is a software that enables In silico siRNA design. The parameters like GC Content and Thermodymics were considered for designing the siRNA sequences against selected mRNA sequences of HER family. The phylogram was also analyzed by constructing the mulpitle sequence alignment using Clustalw to assess the similarity between the siRNA target sequences. Results: The SNPs identified in HER1 , HER2 , HER3 , HER4 family of receptors are missense mutations, any change in nucleotide level are altering the protein sequence structure and function. The mRNA sequences with SNPs positions were submitted to the siRNA Direct 2.0. Set of possible siRNA sequences with 21 nucleotides and the positions where they are targeted. The best possible siRNA sequences were validated using online tools. Conclusion: The siRNA sequences that were identified are considered to be the best suitable for controlling the overexpression of the target mRNA sequences thereby minimizing the development of cancer.
CyTA - Journal of Food, 2014
The primary objective of this study was to extract collagen from underutilized fish species owing... more The primary objective of this study was to extract collagen from underutilized fish species owing to its cost effective nature and also its ability to address the demand of type I collagen arising from food and pharmaceutical industries. Acid and pepsin soluble collagen (ppASC and ppPSC) were extracted from the skin of sucker catfish (Pterygoplichthys pardalis) with a yield of 19.6 and 23.8% on wet weight basis respectively. The same were characterized and confirmed as type I collagen by SDS-PAGE, FTIR and UV-Vis spectroscopy, amino acid analysis, and Zeta potential. Taking into consideration the application of collagen in food industry, a food product was developed by incorporating with fresh cheese. This fortification was found to be acceptable and had not altered the taste, odor and other sensory properties of the product.
Fish Physiology and Biochemistry, 2016
Endocrine disrupting chemicals may induce adverse health effects in humans and wildlife. Recent s... more Endocrine disrupting chemicals may induce adverse health effects in humans and wildlife. Recent studies demonstrate that endocrine disrupting chemicals like Bisphenol A (BPA), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ether (PBDE) affect the reproductive characters shared by wide range of creatures including fish. An attempt was made to evaluate the toxicity of these chemicals on the vitellogenin protein of zebra fish (Danio rerio) using in silico approach. The protein structure of zebra fish vitellogenin was predicted using homology modelling, and the stereochemical quality of the model was validated by Ramachandran plot. The 3-D structure of vitellogenin was docked with the aforementioned chemicals that have demonstrated endocrine-disrupting activity. The pair-wise alignments between vitellogenin with phosvitin, lipovitellin-2 and YGP40 obtained by CLUSTALW alignment suggest that the vitellogenin contained lipovitellin-2- phosvitin- and YGP40-related amino acid sequences. Based on the prediction of CASTp and CLUSTALW, BPA and PCB predominantly interacted with lipovitellin-2 site of the protein, while PBDE interacts predominantly with the YGP40 site of the vitellogenin protein. The results indicate that the endocrine-disrupting chemicals (BPA, PCB and PBDE) dock with the vitellogenin cleavage sites lipovitellin-2 and YGP40 that play a crucial role in lipid-protein complex formation in the egg yolk. We hypothesize that these chemicals could potentially impair the egg yolk formation and eventually impact the zebra fish population which occupies an important niche among testing models used in drug discovery and related toxicity studies.
Molecular Cancer, 2010
Background Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in crucifero... more Background Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in cruciferous vegetables such as brussels sprout and broccoli, is considered a promising chemo-preventive agent against cancer. In-vitro exposure to SFN appears to result in the induction of apoptosis and cell-cycle arrest in a variety of tumor types. However, the molecular mechanisms leading to the inhibition of cell cycle progression by SFN are poorly understood in epithelial ovarian cancer cells (EOC). The aim of this study is to understand the signaling mechanisms through which SFN influences the cell growth and proliferation in EOC. Results SFN at concentrations of 5 - 20 μM induced a dose-dependent suppression of growth in cell lines MDAH 2774 and SkOV-3 with an IC50 of ~8 μM after a 3 day exposure. Combination treatment with chemotherapeutic agent, paclitaxel, resulted in additive growth suppression. SFN at ~8 μM decreased growth by 40% and 20% on day 1 in MDAH 2774 and SkOV-3, respectively....
Laccases (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) are multicopper oxidases that are wide... more Laccases (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) are multicopper oxidases that are widely distributed among plants, insects, and fungi. They belong to a group of polyphenol oxidases containing copper atoms in the catalytic centre which are usually called multicopper oxidases. It utilizes molecular oxygen to oxidize various aromatic and non-aromatic compounds. They have been described in different genera of ascomycetes, some deuteromycetes, and mainly in basidiomycetes. The enzyme catalyze the one electron oxidation of a wide variety of organic and inorganic substrates, including mono-di-and polyphenols, amino-phenols, methoxyphenols, aromatic amines, and ascorbate, with the concamitant four electron reduction of oxygen to water. Laccases are found in Prokaryotes and Eukaryotes. Laccase is localized both intracellular and extracellular fraction. In addition to the general inhibitors a very wide range of compounds are known to inhibit laccase. Fungal laccases typically exhib...
JAMA Surgery, 2014
with chemotherapy, immunotherapy with dendritic cells (DCs) may eliminate minimal disease burden ... more with chemotherapy, immunotherapy with dendritic cells (DCs) may eliminate minimal disease burden by generating cytotoxic T lymphocytes. Enhanced cytosolic bioavailability of tumor-specific antigens improves access to human leukocyte antigen (HLA) class I molecules for more efficient cytotoxic T lymphocyte generation. Various cell-penetrating domains (CPDs) are known to ferry covalently linked heterologous antigens to the intracellular compartment by traversing the plasma membrane. OBJECTIVE To determine whether generating melanoma antigen family A, 3 (MAGE-A3), a tumor-specific cancer-testis antigen, as a fusion protein with CPD will enhance the cytosolic bioavailability of MAGE-A3. DESIGN MAGE-A3 was amplified by polymerase chain reaction using complementary DNA from renal tissue and cloned in frame with a CPD (YARKARRQARR) at the amino-terminal end and hexahistidine at the carboxy-terminal end to generate CPD-MAGE-A3 in a pQE-70 expression vector. Cultures were grown in Escherichia coli BL21 Star (DE3-pLysS) cells followed by nickel-nitrilotriacetic acid affinity purification of recombinant proteins. MAIN OUTCOMES AND MEASURES Measurement of DC membrane penetration of CPD-MAGE-A3 vs MAGE-A3 and determination of the effect of CPD-MAGE-A3 pulsing on DC phenotypic expression of cell-surface antigens. RESULTS Media composition and isopropyl-D-thiogalactosidase induction were optimized to achieve high levels of protein expression followed by purification. Western blot analysis with MAGE-A3 antibodies recognized both MAGE-A3 and CPD-MAGE-A3 proteins, while CPD antibodies recognized only CPD-MAGE-A3. Purified CPD-MAGE-A3 exhibited more efficient DC membrane penetration than did MAGE-A3 alone as confirmed by immunofluorescence analysis. High-level expression of several unique DC markers (CD80, CD83, CD86, and HLA-DR) by flow cytometry was consistent with a mature DC phenotype, indicating that pulsing with CPD-MAGE-A3 did not alter specific cell-surface antigens required for T-cell activation. CONCLUSIONS AND RELEVANCE We have demonstrated for the first time, to our knowledge, that cloning and purification of MAGE-A3 with CPD enhances its cytosolic bioavailability in DCs without altering cell-surface antigens, potentially making it a more potent therapeutic cancer vaccine compared with existing MAGE-A3 protein and peptide vaccines.
Molecular Cancer, 2010
Background Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in crucifero... more Background Sulforaphane (SFN), an isothiocyanate phytochemical present predominantly in cruciferous vegetables such as brussels sprout and broccoli, is considered a promising chemo-preventive agent against cancer. In-vitro exposure to SFN appears to result in the induction of apoptosis and cell-cycle arrest in a variety of tumor types. However, the molecular mechanisms leading to the inhibition of cell cycle progression by SFN are poorly understood in epithelial ovarian cancer cells (EOC). The aim of this study is to understand the signaling mechanisms through which SFN influences the cell growth and proliferation in EOC. Results SFN at concentrations of 5 - 20 μM induced a dose-dependent suppression of growth in cell lines MDAH 2774 and SkOV-3 with an IC50 of ~8 μM after a 3 day exposure. Combination treatment with chemotherapeutic agent, paclitaxel, resulted in additive growth suppression. SFN at ~8 μM decreased growth by 40% and 20% on day 1 in MDAH 2774 and SkOV-3, respectively....
Uploads
Papers by Ravindra Babu Potti