Papers by Maria Clara Cuevas Jaramillo
Tissue Engineering Part C: Methods, 2016
Whole liver engineering holds the promise to create transplantable liver grafts that may serve as... more Whole liver engineering holds the promise to create transplantable liver grafts that may serve as substitutes for donor organs, addressing the donor shortage in liver transplantation. While decellularization and recellularization of livers in animal models have been successfully achieved, scale up to human livers has been slow. There are a number of donor human livers that are discarded because they are not found suitable for transplantation, but are available for engineering liver grafts. These livers are rejected due to a variety of reasons, which in turn may affect the decellularization outcome. Hence, a one-size-fit-for all decellularization protocol may not result in scaffolds with consistent matrix quality, subsequently influencing downstream recellularization and transplantation outcomes. There is a need for a noninvasive monitoring method to evaluate the extent of cell removal, while ensuring preservation of matrix components during decellularization. In this study, we decellularized rat livers using a protocol previously established by our group, and we monitored decellularization through traditional destructive techniques, including evaluation of DNA, collagen, and glycosaminoglycan (GAG) content in decellularized scaffolds, as well as histology. In addition, we used computed tomography and perfusate analysis as alternative nondestructive decellularization monitoring methods. We found that DNA removal correlates well with the Hounsfield unit of the liver, and perfusate analysis revealed that significant amount of GAG is removed during perfusion with 0.1% sodium dodecyl sulfate. This allowed for optimization of our decellularization protocol leading to scaffolds that have significantly higher GAG content, while maintaining appropriate removal of cellular contents. The significance of this is the creation of a nondestructive monitoring strategy that can be used for optimization of decellularization protocols for individual human livers available for liver engineering.
PeerJ, 2016
During the past decade, irreversible electroporation (IRE) ablation has emerged as a promising to... more During the past decade, irreversible electroporation (IRE) ablation has emerged as a promising tool for the treatment of multiple diseases including hepatic cancer. However, the mechanisms behind the tissue regeneration following IRE ablation have not been investigated. Our results indicate that IRE treatment immediately kills the cells at the treatment site preserving the extracellular architecture, in effect causing in vivo decellularization. Over the course of 4 weeks, progenitor cell differentiation, through YAP and notch pathways, together with hepatocyte expansion led to almost complete regeneration of the ablated liver leading to the formation of hepatocyte like cells at the ablated zone. We did not observe significant scarring or tumor formation at the regenerated areas 6 months post IRE. Our study suggests a new model to study the regeneration of liver when the naïve extracellular matrix is decellularized in vivo with completely preserved extracellular architecture.
Psicologia Desde El Caribe Num 1 Vol 31, 2015
Psicologia Desde El Caribe, Jan 29, 2014
ABSTRACT Tesis Univ. Granada. Departamento de Psicología Evolutiva y de la Educación. Leída el 9 ... more ABSTRACT Tesis Univ. Granada. Departamento de Psicología Evolutiva y de la Educación. Leída el 9 de junio de 2006
La intimidación ha sido definida como el uso intencional de la fuerza no ocasional, sino mantenid... more La intimidación ha sido definida como el uso intencional de la fuerza no ocasional, sino mantenida en el tiempo; con el objetivo de generar impacto negativo en la víctima, desde una posición de poder, y por tanto, de desigualdad entre los participantes, tolerado entre el grupo de pares. Como una primera aproximación a la identificación de las prevalencias en el departamento del Valle del Cauca se realizó un primer corte a un estudio de tipo transversal que estima una población de 2.000 escolares de 5º a 10º grados. El primer corte corresponde a una población de 366 escolares, el 32,2% de grado noveno; con un promedio de 13 años [DE+1,82]; en la distribución el 51,4% eran mujeres. Las prevalencias reportan intimidación verbal, 90,1%; social, 86,2%; física, 71,3%, e intimidación por coacción, 29,6%. Estos primeros hallazgos hacen evidente la necesidad de implementar estrategias de intervención para fortalecer las capacidades de afrontamiento frente a las ofensas, burlas y críticas que...
Global Health Promotion, 2015
Background: Student-school connectedness is inversely associated with multiple health risk behavi... more Background: Student-school connectedness is inversely associated with multiple health risk behaviors, yet research is limited on the relative contributions of a student's connectedness with school and an overall context of school social cohesion to peer victimization/bullying. Purpose: We examined associations of perceived school cohesion and student-school connectedness with physical victimization, verbal victimization, and social exclusion in the past six months in adolescents in grades 6-11 (N = 774) attending 11 public and private urban schools in Colombia. Methods: Cross-sectional data were collected via a self-administered questionnaire and analyzed using mixed-effects linear regression models. Results: Higher perceived school cohesion was inversely related with exposure to three bullying types examined (p < 0.05); student-school connectedness was negatively related to verbal victimization among girls only (p < 0.01). In full models, school cohesion maintained inverse associations with three bullying types after controlling for student-school connectedness (p ≤ 0.05). Conclusion: Enhancing school cohesion may hold benefits for bullying prevention beyond a student's individual school connectedness.
Book Reviews by Maria Clara Cuevas Jaramillo
Review of Deliberation across Deeply Divided Societies: Transformative Moments.
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Papers by Maria Clara Cuevas Jaramillo
Book Reviews by Maria Clara Cuevas Jaramillo