Papers by patcharaporn suwor
Plants
Cucurbits are important economic crops worldwide. However, the cucurbit leaf curl disease (CuLCD)... more Cucurbits are important economic crops worldwide. However, the cucurbit leaf curl disease (CuLCD), caused by whitefly-transmitted begomoviruses constrains their production. In Southeast Asia, three major begomoviruses, Tomato leaf curl New Delhi virus (ToLCNDV), Squash leaf curl China virus (SLCCNV) and Squash leaf curl Philippines virus (SLCuPV) are associated with CuLCD. SLCuPV and SLCCNV were identified in Luzon, the Philippines. Here, the genetic diversity and geographic distribution of CuLCD-associated begomoviruses in the Philippines were studied based on 103 begomovirus detected out of 249 cucurbit samples collected from 60 locations throughout the country in 2018 and 2019. The presence of SLCCNV and SLCuPV throughout the Philippines were confirmed by begomovirus PCR detection and viral DNA sequence analysis. SLCuPV was determined as a predominant CuLCD-associated begomovirus and grouped into two strains. Interestingly, SLCCNV was detected in pumpkin and bottle gourd without ...
Crop Breeding and Applied Biotechnology
Tomato Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici (Fol) constrains tomato produ... more Tomato Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici (Fol) constrains tomato production worldwide. Three hundred forty tomato accessions were evaluated for Fusarium wilt resistance and single nucleotide polymorphisms (SNPs) associated with resistance. The disease resistance evaluation revealed that 15, 13, and 15 accessions were identified as Fusarium wilt resistant in Test 1, 2, and Mean data, respectively, with the disease severity index (DSI) ranging from 0-16.7%. A genome-wide association study (GWAS) identified SNPs associated with resistance. Eighteen common SNPs were detected in at least two tests and located on chromosomes 4, 6, 7, 9, and 12. Six unique significant SNPs were found in either Test 1 or 2, located on chromosomes 2, 4, and 7. Candidate genes associated with Fusarium wilt resistance were identified. Notably, two genes encoding leucine-rich repeat-like protein and diseaseresistance protein were predicted from the two unique SNPs, solDsnp10606 and solDsnp6266, respectively.
Journal of Horticultural Research
The tomato (Solanum lycopersicum L.) is one of the most important vegetables grown globally. Howe... more The tomato (Solanum lycopersicum L.) is one of the most important vegetables grown globally. However, the production of tomatoes is restricted by Fusarium oxysporum f. sp. lycopersici (Fol). This study aims to investigate the ability of Fol-resistant tomato genotypes to be a rootstock for the susceptible cultivar. In this study, a tomato cultivar was grafted on rootstocks of the same species (intraspecific), and grafting compatibility, peroxidase gene expression, and fusarium wilt disease incidence of tomato scion was evaluated. A Fol-susceptible tomato ‘Sidathip 3’ (SDT3) was grafted onto four different Fol-resistant tomato genotypes and compared with self-grafted cultivar/cultivar and rootstock/rootstock. The survival rate of all grafted plants was 100% at 20 days after grafting (DAG) without significant differences in incompatibility evaluated at 42 days after grafting. The expression of the peroxidase gene (Solyc02g084800.2) using the qPCR technique was compared in self-grafted ...
International Journal of Agricultural Technology, 2020
Journal of Plant Protection Research, Dec 1, 2021
Pepper yellow leaf curl Thailand virus (PepYLCTHV) causes leaf curl disease in chili production r... more Pepper yellow leaf curl Thailand virus (PepYLCTHV) causes leaf curl disease in chili production regions of the tropics and subtropics. Information on PepYLCTHV disease severity and resistance in chili pepper is still limited in Thailand. This study reports PepYLCTHV disease severity through graft inoculation and selection of single resistant plants for use in a chili breeding program. Twenty-one chili genotypes consisting of the local cultivar (5) collected from Thailand, breeding lines (9) developed at Khon Kaen University (KKU), Thailand and improved lines (7) obtained from the World Vegetable Center, Taiwan were used in this study. Forty-five-day-old seedlings of all the genotypes were graft inoculated with PepYLCTHV in a randomized complete block design (RCBD) with three replications and 10 plants per replication and kept in a plastic net house. Disease symptoms were scored at 20, 27, 34, 41 48, and 55 days after graft/inoculation (DAI). Disease severity was visually recorded using 0−5 scores. Results showed that the disease severity of 21 chili genotypes significantly differed at 48 days after grafting. High resistance and stability were shown by 9853-123 genotypes. Two genotypes, PSP11-7 and PSP11-10-1, showed resistant reaction with disease severity scores of 1.9 and 1.8, respectively. However, among 21 chili genotypes or 630 grafted plants, 302 plants were successfully grafted inoculated plants. Therefore, from the results of this work, highly resistant plants (69 single plants) can be selected, selfed and advanced for breeding.
Scientia Horticulturae, 2017
Inheritance analysis of anthracnose resistance and marker-assisted selection in introgression pop... more Inheritance analysis of anthracnose resistance and marker-assisted selection in introgression populations of chili (Capsicum annuum L.
The Horticulture Journal
Two chili pepper (Capsicum annuum L.) cultivars 'Super Hot' and 'Num Khao' grown under field cond... more Two chili pepper (Capsicum annuum L.) cultivars 'Super Hot' and 'Num Khao' grown under field conditions in Thailand were sprayed with 0 (distilled water, control), 25, 50, 100, and 200 ppm chitosan after one week from transplanting and weekly thereafter for five weeks. Chitosan spray improved vegetative growth of both cultivars as measured by increased canopy size. It also increased the plant height of the 'Super Hot' cultivar and reduced leaf curl incidence in the 'Num Khao' cultivar. Leaf size and chlorophyll content were not significantly affected. Fruit were harvested from cultivars at the commercial maturity stage; that is, the redripe stage for 'Super Hot' and light green stage for 'Num Khao'. From the four harvests at weekly intervals, chitosan treatment increased the 'Super Hot' yield because of greater fruit production and increased fruit size and there was a higher number of fruit per plant produced by the 'Num Khao' cultivar. Fruit color (L*, a*, b*, and sensory color) was not affected, but overall acceptability increased in response to chitosan spray due to improved size and quality of the 'Super Hot' cultivar and improved appearance, size and glossiness of the 'Num Khao' cultivar. Chitosan at 50 ppm appeared to be the optimum concentration to induce the above effects in both cultivars.
The Horticulture Journal
Lime (Citrus aurantiifolia var. Pan) fruits were coated with Aloe vera gel (AVG) at 0 (distilled ... more Lime (Citrus aurantiifolia var. Pan) fruits were coated with Aloe vera gel (AVG) at 0 (distilled water as control), 10, 20, 30, 40, and 50% concentration as a 5 min dip and stored at ambient temperature (29 ± 1°C, 74 ± 5% RH) for 16 days. AVG coating markedly slowed fruit yellowing based on color scores and CIE L*, a*, and b* values, resulting in an increase in shelf life of about four days longer than that of the control (10 days). It did not significantly affect other fruit responses; AVG-coated and uncoated fruit had comparable weight loss, juice content, total soluble solids (TSS), titratable acidity (TA), and vitamin C content. Thus, AVG coating improved shelf life of lime primarily through delayed yellowing without adverse effects on other physicochemical attributes.
European Journal of Plant Pathology, 2015
Anthracnose disease caused by Colletotrichum spp. is a serious disease of chili (Capsicum annuum)... more Anthracnose disease caused by Colletotrichum spp. is a serious disease of chili (Capsicum annuum), particularly in tropical countries. Host plant resistance breeding is one of the most effective disease management strategies. It requires identification of parents with genes resistant to Colletotrichum species predominant in a given region. For phenotypic evaluation, 35 chili lines consisting of progressive lines of C. annuum derived from C. baccatum PBC80 (34 lines) and from C. chinense PBC932 (one line) were inoculated with two aggressive isolates of Colletotrichum acutatum (Ca) and C. capsici (Cc) by injection into the pericarp of green and red-ripe fruit. Lesion diameters were scored at seven days after inoculation. Two simple sequence repeat (SSR) and one sequence characterized amplified region (SCAR) markers were used for validation in four progressive lines and three each of susceptible and resistant checks for genotypic response. The progressive lines were classified into 10 groups based on their responses to two pathogens and at the two fruit stages. Four progressive lines (101, 205, 210 and 215) were selected and used for developing crosses to combine resistance genes from two sources resistant to Ca and Cc at both fruit stages. Progressive lines derived from PBC80 showed DNA fragment 231 bp amplified by primer HpmsE032 associated with Cc at green fruit stages. Hence the HpmsE032 marker could be considered useful in the selection of resistant genotypes derived from PBC80.
Korean Journal of Horticultural Science & Technology, 2013
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Papers by patcharaporn suwor