Papers by maria yamairin acuña padilla
Avances En Psicologia Latinoamericana, Jun 27, 2012
Society of Nuclear Medicine Annual Meeting Abstracts, May 1, 2010
Arch Venez Farmacol Ter, 1998
Avances en Psicologia Latinoamericana
The quality of life of 30 Bell's palsy patients was compared to that of 30 paired controls wi... more The quality of life of 30 Bell's palsy patients was compared to that of 30 paired controls without palsy. The Health Survey Short Form 36 (SF-36) was used to evaluate the quality of life, and the House Brackmann Scale was used to evaluate facial paralysis severity. Participants were also asked about their gender, age, the affected side of the face, and time since the onset of the illness. Wilcoxon test was used to compare the quality of life between patients and paired controls. Results showed significant differences in role-physical (p = .002) and social functioning (p= .002) scales between the two groups. A Spearman's correlation rank test showed that the same two scales of the SF-36 improved in time as the facial paralysis severity diminished (p= .000 and p = .003, respectively). The Mann-Whitney U test showed a lower score in general health scale in patients with right side affected of the face (p=-024). No statistical significant differences were found due to gender, ag...
Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders, 2002
Sarcoidosis occurs most often between 20 and 40 years of age, but also presents in children and o... more Sarcoidosis occurs most often between 20 and 40 years of age, but also presents in children and older adults. Newly diagnosed sarcoidosis in older patients has received little attention. In order to characterize sarcoidosis in older patients, the clinical, radiographic and laboratory features of sarcoidosis presenting in patients aged 50 or older were compared to patients whose sarcoidosis was diagnosed at an earlier age. The medical records of 181 consecutive patients with sarcoidosis were reviewed. They were divided into 92 patients diagnosed at 50 years of age or older (group A), and 89 whose diagnosis preceded age 50 (group B). Comparison of group A with group B revealed that the two groups were similar with regard to race, gender, smoking habits, common presenting symptoms, organ system involvement, pulmonary function data, radiographic stage, PPD status, and laboratory values. At the time of diagnosis, most patients in both groups presented with either respiratory symptoms or ...
Frontiers in Plant Science, 2015
Linolenic acid (Ln) released from chloroplast membrane galactolipids is a precursor of the phytoh... more Linolenic acid (Ln) released from chloroplast membrane galactolipids is a precursor of the phytohormone jasmonic acid (JA). The involvement of this hormone in different plant biological processes, such as responses to biotic stress conditions, has been extensively studied. However, the role of Ln in the regulation of gene expression during abiotic stress situations mediated by cellular redox changes and/or by oxidative stress processes remains poorly understood. An RNA-seq approach has increased our knowledge of the interplay among Ln, oxidative stress and ROS signaling that mediates abiotic stress conditions. Transcriptome analysis with the aid of RNA-seq in the absence of oxidative stress revealed that the incubation of Arabidopsis thaliana cell suspension cultures (ACSC) with Ln resulted in the modulation of 7525 genes, of which 3034 genes had a 2-fold-change, being 533 up-and 2501 down-regulated genes, respectively. Thus, RNA-seq data analysis showed that an important set of these genes were associated with the jasmonic acid biosynthetic pathway including lypoxygenases (LOXs) and Allene oxide cyclases (AOCs). In addition, several transcription factor families involved in the response to biotic stress conditions (pathogen attacks or herbivore feeding), such as WRKY, JAZ, MYC, and LRR were also modified in response to Ln. However, this study also shows that Ln has the capacity to modulate the expression of genes involved in the response to abiotic stress conditions, particularly those mediated by ROS signaling. In this regard, we were able to identify new targets such as galactinol synthase 1 (GOLS1), methionine sulfoxide reductase (MSR) and alkenal reductase in ACSC. It is therefore possible to suggest that, in the absence of any oxidative stress, Ln is capable of modulating new sets of genes involved in the signaling mechanism mediated by additional abiotic stresses (salinity, UV and high light intensity) and especially in stresses mediated by ROS.
Current Treatment Options in Gastroenterology, 2006
Thyroid, 1997
We present the unusual case of a 29-year-old man diagnosed in 1975 with papillary carcinoma of th... more We present the unusual case of a 29-year-old man diagnosed in 1975 with papillary carcinoma of the thyroid metastatic to regional lymph nodes. The patient underwent surgical resection, postoperative iodine-131 (131I) radioablation and levothyroxine suppression. He was subsequently lost to follow-up. In 1991, he presented with extensive metastatic disease that was not demonstrable on whole-body 131I imaging, but was seen on computerized tomography and whole-body thallium chloride scanning. The patient was treated with cisplatin (Platinol) and doxorubicin (Adriamycin). Repeat 131I imaging after three cycles of chemotherapy showed significant 131I uptake in previously non-iodine-concentrating lesions. The patient was subsequently treated with 200 mCi 131I. We postulate this patient's non-iodine-concentrating thyroid cancer may have become functional by either a differentiating effect of chemotherapy on the tumor cells, or perhaps a selective cytotoxicity against nonfunctional, less differentiated papillary thyroid cancer cells, or both. This would allow more functional differentiated cells to overgrow and become the predominant cell type in the lesions. Chemotherapy may be beneficial in patients with advanced non-iodine-concentrating differentiated thyroid carcinoma by inducing radioiodine uptake and allowing subsequent radioiodine therapy. The possible mechanisms of induction of iodine uptake by chemotherapy are discussed.
Mount Sinai Journal of Medicine: A Journal of Translational and Personalized Medicine, 2009
The interstitial lung diseases are a heterogeneous group of disorders characterized by inflammati... more The interstitial lung diseases are a heterogeneous group of disorders characterized by inflammation and/or fibrosis of the pulmonary interstitium. In 2002, the American Thoracic Society and the European Respiratory Society revised the classification of interstitial lung diseases and introduced the term diffuse parenchymal lung disease. The idiopathic interstitial pneumonias are a subtype of diffuse parenchymal lung disease. The idiopathic interstitial pneumonias are subdivided into usual interstitial pneumonia (with its clinical counterpart idiopathic interstitial pneumonia), nonspecific interstitial pneumonia, cryptogenic organizing pneumonia, acute interstitial pneumonia, desquamative interstitial pneumonia, respiratory bronchiolitis interstitial lung disease, and lymphocytic pneumonia. Sarcoidosis and hypersensitivity pneumonitis are the 2 most common granulomatous diffuse parenchymal lung diseases. Rheumatoid arthritis, systemic sclerosis, and dermatomyositis/polymyositis (causing antisynthetase syndrome) are diffuse parenchymal lung diseases of known association because these conditions are associated with connective tissue disease. Hermansky-Pudlak syndrome is a rare genetic diffuse parenchymal lung disease characterized by the clinical triad of pulmonary disease, oculocutaneous albinism, and bleeding diathesis. This review provides an overview of the chronic fibrosing diffuse parenchymal lung diseases. Its primary objective is to illuminate the clinical challenges encountered by clinicians who manage the diffuse parenchymal lung diseases regularly and to offer potential solutions to those challenges. Treatment for the diffuse parenchymal
Journal of Experimental Botany, 2013
Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have beco... more Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have become a new area of research, as they can modulate the function of target proteins. Proteomic data have shown that ascorbate peroxidase (APX) is one of the potential targets of PTMs mediated by NO-derived molecules. Using recombinant pea cytosolic APX, the impact of peroxynitrite (ONOO-) and S-nitrosoglutathione (GSNO), which are known to mediate protein nitration and S-nitrosylation processes, respectively, was analysed. While peroxynitrite inhibits APX activity, GSNO enhances its enzymatic activity. Mass spectrometric analysis of the nitrated APX enabled the determination that Tyr5 and Tyr235 were exclusively nitrated to 3-nitrotyrosine by peroxynitrite. Residue Cys32 was identified by the biotin switch method as S-nitrosylated. The location of these residues on the structure of pea APX reveals that Tyr235 is found at the bottom of the pocket where the haem group is enclosed, whereas Cys32 is at the ascorbate binding site. Pea plants grown under saline (150 mM NaCl) stress showed an enhancement of both APX activity and S-nitrosylated APX, as well as an increase of H 2 O 2 , NO, and S-nitrosothiol (SNO) content that can justify the induction of the APX activity. The results provide new insight into the molecular mechanism of the regulation of APX which can be both inactivated by irreversible nitration and activated by reversible S-nitrosylation.
Chest, 2005
To differentiate the clinical, radiographic, and physiologic profile in patients with sarcoidosis... more To differentiate the clinical, radiographic, and physiologic profile in patients with sarcoidosis with and without pulmonary hypertension. Design: Retrospective survey. Setting: Tertiary care center. Patients: One hundred six patients with sarcoidosis were classified by two-dimensional echocardiography into two groups: group 1, 54 patients with pulmonary hypertension; group 2, 52 patients without pulmonary hypertension. Interventions: Patients underwent two-dimensional and Doppler echocardiography, chest radiography (CXR), pulmonary function testing, and arterial oxygen saturation determination, and the test results were compared between the two groups. Statistical analysis was performed using independent-sample t test and 2 test, as appropriate; p < 0.05 was considered to be significant. Results: Predicted spirometric values and lung diffusing capacity were significantly lower in patients in group 1 compared to patients in group 2: FVC, 54% vs 64% (p ؍ 0.0065), FEV 1 , 47% vs 61% (p ؍ 0.0005), forced expiratory flow, midexpiratory phase, 35% vs 52% (p ؍ 0.0363), and single-breath diffusing capacity of the lung for carbon monoxide (DLCOsb), 39% vs 54% (p ؍ 0.0001). Sixty percent of patients in group 1 had radiographic Scadding stage 4 sarcoidosis, while no radiographic stage predominated in group 2. Arterial oxygen saturation, need for oxygen supplementation, and degree of desaturation after exercise did not differ between groups. Conclusions: The presence of pulmonary hypertension in patients with sarcoidosis is associated with higher prevalence of stage 4 sarcoidosis by CXR and lower predicted spirometric and DLCOsb measurements.
Chest, 2001
Background: The incidence of pulmonary complications in heart transplant recipients has not been ... more Background: The incidence of pulmonary complications in heart transplant recipients has not been extensively studied. We report pulmonary complications in 159 consecutive adult orthotopic heart transplantations (OHTs) performed in 157 patients. Materials and methods: Retrospective review of medical records. Results: Forty-seven of 159 recipients (29.9%) had 81 pulmonary complications. Pneumonia was the most common (n ؍ 27), followed by bronchitis (n ؍ 15), pleural effusion (n ؍ 10), pneumothorax (n ؍ 7), prolonged respiratory failure requiring tracheotomy (n ؍ 7), and obstructive sleep apnea syndrome (n ؍ 6). All patients with late-onset (> 6 months after transplantation) community-acquired bacterial pneumonia presented with fever, cough, and a new lobar consolidation on the chest radiograph, and responded promptly to empiric antibiotics without undergoing an invasive diagnostic procedure. In contrast, early-onset nosocomial bacterial pneumonias carried a 33.3% mortality rate. A positive tuberculin skin test result was associated with a significantly higher rate of pulmonary complications (62.5% vs 26.8%, p ؍ 0.007). Lung cancer and posttransplant lymphoproliferative disorder (PTLD) developed exclusively in 6 of the 61 patients (8.1%) who received induction immunosuppression with murine monoclonal antibody (OKT3). Conclusion: Pulmonary complications are common following heart transplantation, occurring in 29.9% of recipients, and are attributed to pneumonia of primarily bacterial origin in one half of cases. Late-onset community-acquired pneumonia carried an excellent prognosis following empiric antibiotic therapy, suggesting that in the appropriate clinical setting invasive diagnostic procedures are unnecessary. Analogous to reports in other solid-organ transplant recipients, induction therapy with OKT3 was associated with an increased incidence of lung cancer and PTLD. Overall, the development of pulmonary complications after OHT has prognostic significance given the higher mortality in this subset of patients.
CHEST Journal, 2006
PURPOSE: PET scans are accepted as valuable aides in the diagnosis and staging of neoplastic dise... more PURPOSE: PET scans are accepted as valuable aides in the diagnosis and staging of neoplastic diseases. However, FDG avidity is common also in granulomatous diseases and PET scanning has been reported to be positive in a growing number of patients with sarcoidosis. METHODS: We have performed total body PET scans in 148 patients with biopsy proven sarcoidosis. RESULTS: 98 had at least one site which was positive as evidenced by increased FDG uptake, with SUV's form 2-8. The following organs with PET positivity were identified: lungs 38, mediastinal lymph nodes 46, peripheral lymph nodes 36, abdominal lymph nodes 27, lacrimal and parotid glands 26, bones 14, liver and spleen 11, miscellaneous 16. CONCLUSION: Many of the total body PET scans were performed at the time of Rb-80 and FDG scans seeking evidence of cardiac sarcoidosis. Total body PET scan positivity was common in patients with other clinical evidence of active granulomatous lesions while the scans tended to be negative or low SUV in patients with radiographic stable or fibrotic pulmonary sarcoidosis. There was no correlation with serum angiotensin converting enzyme levels. CLINICAL IMPLICATIONS: Total body FDG PET scans for sarcoidosis were most valuable in: 1.identifying possible diagnostic biopsy sites2.as a guide to therapy in patients with evidence of pulmonary fibrosis (stage IV radiographs).
Biochimica et Biophysica Acta (BBA) - General Subjects, 2013
Background: Protein tyrosine nitration is a post-translational modification (PTM) mediated by nit... more Background: Protein tyrosine nitration is a post-translational modification (PTM) mediated by nitric oxide-derived molecules. Peroxisomes are oxidative organelles in which the presence of nitric oxide (NO) has been reported. Methods: We studied peroxisomal nitroproteome of pea leaves by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) and proteomic approaches. Results: Proteomic analysis of peroxisomes from pea leaves detected a total of four nitro-tyrosine immunopositive proteins by using an antibody against nitrotyrosine. One of these proteins was found to be the NADH-dependent hydroxypyruvate reductase (HPR). The in vitro nitration of peroxisomal samples caused a 65% inhibition of HPR activity. Analysis of recombinant peroxisomal NADH-dependent HPR1 activity from Arabidopsis in the presence of H 2 O 2 , NO, GSH and peroxynitrite showed that the ONOO − molecule caused the highest inhibition of activity (51% at 5 mM SIN-1), with 5 mM H 2 O 2 having no inhibitory effect. Mass spectrometric analysis of the nitrated recombinant HPR1 enabled us to determine that, among the eleven tyrosine present in this enzyme, only Tyr-97, Tyr-108 and Tyr-198 were exclusively nitrated to 3-nitrotyrosine by peroxynitrite. Site-directed mutagenesis confirmed Tyr198 as the primary site of nitration responsible for the inhibition on the enzymatic activity by peroxynitrite. Conclusion: These findings suggest that peroxisomal HPR is a target of peroxynitrite which provokes a loss of function. General significance: This is the first report demonstrating the peroxisomal NADH-dependent HPR activity involved in the photorespiration pathway is regulated by tyrosine nitration, indicating that peroxisomal NO metabolism may contribute to the regulation of physiological processes under no-stress conditions.
American Journal of Therapeutics, 2007
Diuretics, which are primarily used to modify the volume and the composition of body fluids, are ... more Diuretics, which are primarily used to modify the volume and the composition of body fluids, are widely used to treat hypertension. The diuretics include a) the thiazides and thiazide-like agents, which are the most common drugs used to treat high blood pressure (these drugs inhibit sodium reabsorption in the early distal convoluted tubule); b) loop diuretics, such as furosemide, block chloride and sodium reabsorption by inhibition of the Na + /K + /2Cl 2 cotransport system in the thick ascending limb of the loop of Henle; and c) potassium-sparing (retaining) diuretics, including aldosterone receptor blockers (such as spironolactone and eplerenone) and epithelial sodium channel blockers (such as amiloride and triamterene, which interfere with the reabsorption of sodium and excretion of potassium and hydrogen that takes place in the late distal tubule, the connecting tubule, and the cortical collecting duct). Hydrochlorothiazide 12.5 mg once daily or equivalent low dosages of other similar agents reduce blood pressure in approximately one-half to two-thirds of patients who are responsive to this class of drugs; higher doses add little to the effect on blood pressure and also increase side effects. Some combinations of very small doses of thiazide diuretics-for example, 6.25 mg hydrochlorothiazide or 0.625 mg indapamide, with a low dose of an antihypertensive drug of a different class-have average antihypertensive efficacy when used once daily. Furosemide is used in patients with renal failure or severe heart failure and is best given by continuous intravenous infusion. The potassium-sparing diuretics are generally used in combination with thiazide diuretics to treat hypertension.Side effects occur at about the same frequency and severity with equipotent doses of all diuretics. The incidence of side effects is dose-dependent and also increases as a function of the duration of the renal excretory and antihypertensive actions. However, longer-acting diuretics provide better 24-hour control of blood pressure and increase compliance and adherence to the treatment regimen.
American Heart Journal, 1991
The risk of complications caused by hypertension depends on several factors, including systolic (... more The risk of complications caused by hypertension depends on several factors, including systolic (SBP) and diastolic blood pressure (DBP) levels, blood lipid levels, hyperglycemia, smoking, and others. Platelet and endothelial functions are also involved in the pathophysiology of ischemic heart disease,lV3 and several studies have reported a very high platelet aggregability in hypertensive patients.4s 5 By treating hypertension pharmacologically, we can reduce the incidence of cerebrovascular accidents and death, as well as preventing heart and renal failure. The Framingham Heart Study has shown that in general antihypertensive drug therapy reduces cardiovascular and cerebrovascular mortality end points in patients with varying degrees of hypertension and also reduces cardiovascular complications significantly.6-8 However, antihypertensive treatment modifies coronary heart disease (CHD) morbidity and mortality rates only minimally.l, g
American Heart Journal, 1991
An in vitro assay was used to investigate the effects of doxarosin on the platelet aggregation in... more An in vitro assay was used to investigate the effects of doxarosin on the platelet aggregation induced by epinephrine, collagen, and adenosine diphosphate. Platelet-rich plasma from normotensive subjects and patients with hypertension was compared. Doxatosin produced a concentration-dependent inhibition of platelet aggregation in both groups, but significantly lower concentrations were required to inhibit platelet aggregation in plasma taken from patients with hypertension. The concentrations of doxarosin that inhibited platelet aggregation in vitro were similar to those that are used clinically to control blood pressure in patients with hypertension.
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Papers by maria yamairin acuña padilla