Papers by Irina Nakashidze
Journal of perinatology : official journal of the California Perinatal Association, Jan 18, 2015
To evaluate the effects of gestational subclinical hypothyroidism (SCH) on early neurodevelopment... more To evaluate the effects of gestational subclinical hypothyroidism (SCH) on early neurodevelopment of offspring. A prospective study included 106 infants born to mothers with gestational SCH and 106 infants born to mothers who were euthyroid during pregnancy. The neurodevelopment of 12 to 24-month-old infants was assessed and compared using the Gesell developmental test (revised version). Infants born to mothers with gestational SCH and those born to euthyroid mothers had similar scores on the Gesell development test. No correlations were observed between maternal TSH concentration and Gesell developmental test scores of offspring. Infants born to mothers who had gestational SCH during the first trimester specifically and those born to euthyroid mothers had similar scores on the Gesell development test. No significant correlations were detected between maternal TSH concentration during the first trimester and offspring neurodevelopment. No detectable neurodevelopment deficit was obse...
IJEB Vol.59(05) [May 2021], May 1, 2021
Prostate cancer is one of the most common cancers in men worldwide. Therefore, identification of ... more Prostate cancer is one of the most common cancers in men worldwide. Therefore, identification of specific biomarkers for early dignosis are crucial prerequisites for efficient treatment of patients. We investigated alterations in the physicochemical characteristics of blood plasma proteins from men with prostate tumors, and its diagnostic significance with the identified alterations. Blood plasma of patients with benign hyperplasia of the prostate (BHP), BHP with high-grade prostatic intraepithelial neoplasia (HGPIN) regions and Cancer of Prostate (CaP) were used. Spectroscopic and SDS-PAGE methodologies were utilized for examining alterations in the physicochemical characteristics of blood plasma proteins. Relationship between alterations of the first-peak fluorescence intensities and prostate tumor progression was observed. Also, increased intensity of the second-peak corresponding to nicotinamide co-enzymes (NADH and NADPH) was noted in BHP-with-HGPIN and CaP specimens. The main peak maximum (Td) was observed at 66-67°C in BHP plasma and at 63-64°C in BHP-with-HGPIN regions and CaP. In BHP and BHP-with-HGPIN regions, an arm was noted at 70-71°С. The portions of thermostable proteins (acute phase proteins) were increased and modified proteins were formed in plasmas during the malignant transformation. Recorded fluorescence spectra allowed to differentiate prostate tumors and determine the disease progression. Differential scanning calorimetry markedly differentiated benign BHP-with-HGPIN regions and CaP, which shows the diagnostic importance of the method.
Obstetrics, Gynecology and Reproduction, 2022
Introduction. Autoimmune thyroiditis (AIT) is the most prevalent among the autoimmune pathologies... more Introduction. Autoimmune thyroiditis (AIT) is the most prevalent among the autoimmune pathologies and multifactorial thyroid diseases; accordingly, remaining a pressing issue for investigation.Aim: to study AIT profile in different age groups of the female population in the western region of Georgia – Adzhariya.Materials and Methods. A prospective comparative study in parallel groups was carried out. There were examined 405 samples of venous blood obtained at «Health» Clinic from women of two groups: group 1 – 159 patients with AIT, group 2 (control) – 246 women without AIT. Blood samples were collected between the years 2017 and 2020. There were analyzed serum antibodies against thyroperoxidase (TPOAb), thyroid stimulating hormone (TSH) as well as level of free thyroxine (FT4) by using enzyme immunoassay.Results. Patients with AIT from different age groups vs. control group were found to have decreased level of FT4, but increased amount of TSH and TPOAb. At the reproductive age (18...
Critical Reviews™ in Oncogenesis, 2020
Colon cancer (CC) belongs to a widespread malignant progression from the digestive tract. Moreove... more Colon cancer (CC) belongs to a widespread malignant progression from the digestive tract. Moreover, CC is the leading cause of cancer-related mortality worldwide. It was suggested that there are several causative risk factors, among which are genetic factors that play an important role in the predisposition and development of CC. Unfortunately, despite continuous vigorous research going back more than two decades regarding molecular genetics and epigenetics on the issues of CC, there were no suitable prognostic and predictive molecular biomarkers identified until now. There are several susceptible genes for CC. Researchers are still unsure about the mechanisms regarding the genetic predisposition and single nucleotide polymorphisms (SNPs). The SNPs are considered to be clinically important aspotential diagnostics and therapeutic biomarkers in the future. Thus, in this review, we revise some gene SNPs involved in inducing CC.
Gynecologic Oncology, 2020
Journal of Biotechnology, 2019
Journal of Biotechnology, 2019
Journal of Biotechnology, 2019
The genus Bunium L. is represented by approximately 30 species distributed in West and South Asia... more The genus Bunium L. is represented by approximately 30 species distributed in West and South Asia, Europe and North Africa. In Turkey, the genus contains 12 species and 18 taxa. Bunium elegans (Fenzl) Freyn has considerable variation in bract, bracteole, pedicel, fruit and habit. This served as an justification for identifying many of its forms, some of which were described as independent taxa or species. This situation also caused the independent species to be included in B. elegans. The latter species was divided into five varieties, var. elegans, var. brevipes Freyn & Sint., var. latilobum Saya, var. pachypus Saya and var. involucratum Saya, in Turkey. The aim of the present study was to determine phylogenetic relationship among the taxa of B. elegans and related species using nrDNA-ITS sequence. Our results show that Bunium elegans var. latilobum should be raised to the species rank. Bunium elegans var. involucratum is strictly related to B. simplex (K. Koch) Kljuykov. Our molecular investigation provides further support for regarding Bunium simplex and B. elegans as separate species. Bunium elegans var. pachypus is closely related to B. verruculosum Towns. In conclusion, the nrDNA-ITS data has been provided valuable contributions to the taxonomy of the Bunium elegans complex.
Journal of Biotechnology, 2019
E-Poster viewings, 2019
Results Among 150 HGSC samples, we identified 44 samples (29.3%) with reportable variants with va... more Results Among 150 HGSC samples, we identified 44 samples (29.3%) with reportable variants with variant allele frequencies from 10.3%-99.4%. These included 35 point mutations/ insertions/deletions, 7 exon/whole gene deletions, and 2 BRCA1 exon 13 duplications. A subset (26) of these variants were then confirmed by targeted assays using Sanger and MLPA. Conclusions Utilizing NGS technology, we reliably identified BRCA mutations in FFPE tumor samples. A validated NGS pipeline provides a valuable clinical tool to conduct Traceback initiatives to the families of deceased ovarian cancer patients never tested for germline mutations.
E-Poster viewings, 2019
Objectives Mozambique has a high prevalence of gynecologic cancers and has no trained gynecologic... more Objectives Mozambique has a high prevalence of gynecologic cancers and has no trained gynecologic oncologist or specialized training program. There are challenges associated with creating a training program. Methods The International Gynecologic Cancer Society (IGCS) Gynecologic Oncology Global Curriculum & Mentorship Program, a two-year program to train gynecologists in gynecologic oncology in countries without training programs,
International Journal of Health Services Research and Policy, 2019
Frailty is defined as "medical syndrome with multiple causes and contributors that is characteriz... more Frailty is defined as "medical syndrome with multiple causes and contributors that is characterized by diminished strength, endurance, and reduced physiologic function that increases an individual's vulnerability for developing increased dependency and/or death. An assessment of Frailty in various populations, especially in the elderly population, has medical outcomes. The present work deals with the study of frailty in the beneficiaries of Batumi St. Catherine Nursing Home of. In total 70 beneficiaries of both sexes, were over 60 years of age, were investigated. The study was conducted in two years 2016-2017. Frailty phenotypes were assessed within five criteria: weight loss, self-reported exhaustion, weakness, slowness, and low physical activity. The existence of three and more criteria from these listed criteria are assessed as frail phenotype; the existence of one or two criteria-prefrail, and if a person does not have any criteria, it is assessed as robust. 25.7% of the elder beneficiaries turned out to be frailty phenotype, 12,9 %-prefrail phenotype, 61.4%-healthy or sustainable. Total frailty phenotype was identified in 18 samples, including 11 women and 7 men. Hard cognitive disorders from the 18 frailty phenotypes were found in only 3 individuals. Among the three most commonly researched populations in the frailty criteria are low physical activity, weakness, and slowness. In the group of people older than 80 years, the syndrome of frailty was higher (35.7%) than in the group of people older than 60 years. (19%). The gender difference was not observed. As the research has shown frailty syndrome is revealed in beneficiaries in both investigated groups. It is important to pay special attention to assess frailty syndrome in the nursing community, to be used timely medical, social or other activities.
Gynecologic Oncology, 2019
Objective: AMP-activated protein kinase (AMPK) is a highly conserved serine/threonine protein kin... more Objective: AMP-activated protein kinase (AMPK) is a highly conserved serine/threonine protein kinase and an essential mediator in maintaining cellular energy homeostasis. Recent studies suggested that AMPK is crucial for the activation of autophagy, which is an emerging target for the prevention and treatment of various cancers. The aim of this study was to explore the antitumor effects of AMPK-inducing agents in human cervical cancer cells. Method: Human cervical cancer cell lines HeLa and SiHa were cultured. Autophagic cell death, intrinsic apoptosis and total apoptosis, and survival fraction were investigated after treatment with AMPK inducer. Results: We found that AMPK-inducing agents including metformin, fenofibrate, or 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) decreased the cell viability of HeLa cells in a dose-and time-dependent manner. Treatment of AICAR or fenofibrate significantly activated the AMPK-signaling pathway, leading to subsequent induction of autophagy. However, pretreatment of autophagy-specific inhibitors markedly abrogated AICAR-or fenofibrate-mediated cytotoxic effects. Conclusion: Collectively, our results suggested that the application of AMPK-inducing agents is a promising strategy for the prevention and treatment of cervical cancer and the activation of autophagy.
Gynecologic Oncology, Oct 1, 2020
Clinical and Applied Thrombosis-Hemostasis, Feb 13, 2018
Methylenetetrahydrofolate reductase (MTHFR) gene polymorphism (C677T)] is a well-recognized genet... more Methylenetetrahydrofolate reductase (MTHFR) gene polymorphism (C677T)] is a well-recognized genetic risk factor for venous thrombosis; however, its association with arterial thrombosis is still under debate. Herein, we evaluated the prevalence of MTHFR C677T polymorphism in Georgian patients in comparison with healthy individuals and its association with arterial thrombosis. We enrolled 214 participants: 101 with arterial thrombosis (71.3% males; mean age: 66.3 + 12.1 years) and 113 controls (67.3% males; mean age: 56.6 + 11.3 years). Genomic DNA was extracted from dry blood spot on Whatman filter paper. Polymerase chain reaction was performed to determine MTHFR C677T polymorphism. Frequency of C677T allele polymorphism in controls was 21.2%, which corresponded to heterozygous and homozygous stage frequencies of 35.4% and 3.5%, respectively. In patient group, an allelic frequency of 33.2% was found, which corresponded to the presence of 48.5% of heterozygous and 8.9% of homozygous individuals. Comparing the frequency of mutated alleles between the 2 groups, a significantly high frequency of mutated alleles was found in patient group (P < .05). In conclusion, high frequency of MTHFR C677T polymorphism found in arterial thrombosis patient group suggests that this polymorphism might increase the risk of arterial thrombosis in Georgian patients.
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Papers by Irina Nakashidze