Papers by alejandro azaola
Journal of Industrial Microbiology & Biotechnology, Nov 1, 2002
Oligosaccharide (OS) production, cell concentration (2Â Â Â Â Â Â Â10 9 colony-forming unit / ml)... more Oligosaccharide (OS) production, cell concentration (2Â Â Â Â Â Â Â10 9 colony-forming unit / ml), lactose concentration (25% wt / vol), reaction time (6 h), and temperature (508 8 8 8C) were chosen as the central condition of the central composite design (CCD) for optimizing the production process using Bifidobacterium infantis RW-8120 in skim milk. Statistical analysis (P < 0.01) revealed that the most relevant variable concerning OS production and yield was the lactose concentration. The coefficient of determination (R 2) is good for the second-order OS production model (0.92) and fairly good for the second-order nonlinear OS yield model (0.816). An increase of lactose concentration and temperature resulted in a higher OS production. The optimal values for OS production appear to be near the area associated with the central points of the modeling design except for the lactose concentration, which was 40% (wt / vol) of the final volume.
International Journal of Food Microbiology, 2003
Revista Mexicana de Ciencias Farmaceuticas
Resumen La obesidad es el resultado de un desequilibrio entre ingesta y gasto de energía, más la ... more Resumen La obesidad es el resultado de un desequilibrio entre ingesta y gasto de energía, más la información genética y el estilo de vida se genera un incremento en el peso y la grasa corporal. México ocupa el primer lugar en obesidad con una prevalencia de 33 %. La atención médica para un individuo obeso representa el doble de gasto económico que para una persona sana. Esta patología se encuentra relacionada con enfermedades cardiovasculares, hipertensión, cáncer y diabetes mellitus tipo 2. Últimamente, la obesidad se ha relacionado con la microbiota intestinal y una proporción alterada de dos grupos microbianos dominantes; los Firmicutes y los Bacteroidetes. Este desbalance a favor de los Firmicutes, considerados como extractores de energía por sus actividades enzimáticas y junto a la alta ingesta de alimentos, se considera como factores que in uyen en la obesidad de los individuos.
Introducción. Las bifidobacterias son habitantes normales del tracto intestinal humano, en los úl... more Introducción. Las bifidobacterias son habitantes normales del tracto intestinal humano, en los últimos 20 años se ha incrementado el interés comercial y científico en el género Bifidobacterium por el gran número de efectos benéficos que se le ha atribuido ya que estas bacterias, combaten enfermedades diarreicas, mejoran la intolerancia a la lactosa, ayudan a combatir infecciones intestinales, colitis ulcerativa, estreñimiento, estimulan el sistema inmune, reducen colesterol en sangre e inclusive juegan un papel muy importante en la prevención del cáncer de colon (1). Estos microorganismos utilizan una gran variedad de carbohidratos como fuente de carbono, algunos como lactosa, sacarosa, glucosa, galactosa y fructosa son utilizados por prácticamente todas las especies de bifidobacterias, otros como arabinosa, xilosa y manosa son utilizados sólo por algunas especies Es importante resaltar que ciertos carbohidratos como oligo-fructosa, inulina y rafinosa son promotores específicos del ...
Revista Brasileira de Zootecnia, 2013
Sixty New Zealand rabbits aged 40 days were divided into 4 groups of 15 animals. The control grou... more Sixty New Zealand rabbits aged 40 days were divided into 4 groups of 15 animals. The control group received a free diet of antibiotic growth promoter (AGP) and inulin. The second group was supplemented with 2.5 g/kg of inulin. The third was administered AGP with 0.1 g/kg of flavomycin. Finally, the fourth group received a 2.5 and 0.1 g/kg inulin/AGP diet. Body weight gain was higher in the control group. Rabbits supplemented with inulin had lower values of triglycerides compared with the control and AGP groups, and their glucose level was significantly lower than those treated with AGP. Additionally, serum calcium and magnesium concentrations were higher than the other groups, particularly with regard to AGP. The bone content with regard to calcium, phosphorus and magnesium in the groups treated with inulin was higher compared with the control; moreover, phosphorus and magnesium were higher than in the AGP group. The thickness of the mucosa and crypt depth in the caecum were significantly higher in rabbits treated with inulin than in the other groups, but especially compared with the AGP group. Also, triglyceride values were lower for rabbits treated with inulin/AGP than for those treated with AGP and the bone magnesium concentration was significantly higher compared with the control group. In addition, inulin was shown to have positive effects on the rabbit, promoting increase in bone and serum calcium, magnesium and phosphorus, decrease in triglyceride levels, and improvement in the caecum (changes in morphology, crypt depth and mucosal thickness).
Biotechnology Techniques, 1999
An optimized medium containing Trypticasein, phytone, yeast extract and glucose is proposed to im... more An optimized medium containing Trypticasein, phytone, yeast extract and glucose is proposed to improve growth of bifidobacteria with high yeast extract concentration and decreased amounts of Trypticasein and phytone. These new growth media overcome nutritional limitations by the type and amount of amino acids contained in these sources and produced an increase from 1.8 to more than 4 g cell mass
Process Biochemistry, 2001
A suspension of Kluyveromyces marxianus cells (256 mg ml−1) with inulinase activity were immobili... more A suspension of Kluyveromyces marxianus cells (256 mg ml−1) with inulinase activity were immobilized in barium alginate treated with glutaraldehyde. After five runs of inulin hydrolysis, the immobilized cells system had up to 85% residual activity. When beads were heat treated at 65°C for 5 min, the hydrolysis rate of inulin was improved without affecting the stability of the system
Journal of Industrial Microbiology and Biotechnology, 2002
International Journal of Food Microbiology, 2003
Microencapsulated cells of Bifidobacterium longum B6 and Bifidobacterium infantis CCRC 14633 were... more Microencapsulated cells of Bifidobacterium longum B6 and Bifidobacterium infantis CCRC 14633 were prepared by spray drying the cell suspension containing the test organism and 10% (w/w) of the carrier material of either gelatin, soluble starch, skim milk or gum arabic. Survival of these microencapsulated and free cells of bifidobacteria in simulated gastric juice (pH 2.0 and 3.0) and bile solution (0.5% and 2.0%) was then examined. B. infantis CCRC 14633 was more susceptible than B. longum B6 to the simulated gastric environment and bile solution tested. Microencapsulated bifidobacteria exhibited a lower population reduction than free cells during exposure to simulated gastric environment and bile solution. This phenomenon was most pronounced when the test organism was exposed to gastric juice at pH 2.0 or 2.0% bile solution. Moreover, it was also observed that the protective effect exerted by encapsulation with spray drying varied with the carriers used and the strains of bifidobacteria.
Uploads
Papers by alejandro azaola