The Journal of Plant Science Research, Feb 10, 2023
Rhododendron campanulatum D.Don belongs to family Ericaceae, is an ornamental and medicinal plant... more Rhododendron campanulatum D.Don belongs to family Ericaceae, is an ornamental and medicinal plant which is commonly known as ‘Nichnai’ in Kashmir. During the present study, in vitro propagation strategies were used for micropropagation of Rhododendron campanulatum. Seeds of R. campanulatum were inoculated on Anderson Rhododendron basal medium (ARM) and also ARM supplemented with various auxins and cytokinins in different combinations and concentrations. Seed germination was observed on Anderson Rhododendron basal medium (ARM) and ARM + BAP (3mg/l) within 35.34 and 30.42 days of culture respectively. Seed germination was also observed on basal WPM as well as on WPM +2,4-D (4mg/l), WPM+BAP (1mg/l), WPM+BAP (2mg/l) respectively. In addition, callus production as well as shoot formation was observed on ARM supplemented with 2,4-D (3 and 4 mg/l) within 56.41 and 70.16 days respectively. Callus obtained from seed explants was subcultured on WPM supplemented with different cytokinins having different combinations. Among different cytokinin-cytokinin combinations used, WPM+2ip(2mg/l)+TDZ (4mg/l) induced maximum mean number 4.6±0.3 of shoots. The in vitro raised plantlets were acclimatized using cocopeat & perlite having different ratios as well as in garden soil under greenhouse conditions.
Journal of scientific and innovative research, Dec 25, 2014
Bunium persicum, family Apiaceae is a native of west Asia and has a limited distribution. During ... more Bunium persicum, family Apiaceae is a native of west Asia and has a limited distribution. During the present study an efficient in vitro protocol has been standardized viz, callus production from cotyledon, hypocotyl and nodal explants. Callus was obtained from these explants on MS medium fortified with different growth regulators both auxins (IAA, IBA, NAA, 2,4-D) and cytokinins (BAP and Kn). From cotyledon explants best callus production was achieved on MS medium supplemented with BAP (2 mg/l) +IAA (2 mg/l) within 10 days. For hypocotyls explants MS medium fortified with BAP (2 mg/l) resulted in best callus production within 24 days. MS medium supplemented with BAP (1 mg/l) +IAA (3 mg/l) proved to be most effective in callus production from nodal explants within 19 days.
Journal of scientific and innovative research, Oct 25, 2014
Rheum webbianum Royle is an important medicinal plant of family Polygonaceae growing in Kashmir H... more Rheum webbianum Royle is an important medicinal plant of family Polygonaceae growing in Kashmir Himalaya. It possesses hepatoprotective, spasmolytic, anticholesterolaemic, antitumor, antiseptic, antifungal, anti-microbial, anti-Parkinson's, anti-proliferative, immuno-enhancing, antiviral and antioxidant properties. During the present study an attempt has been made to standardize a protocol for large scale propagation of this threatened Himalayan plant species using rhizome cuttings as explants. Callus was obtained when the rhizome explants were inoculated on MS medium containing different concentrations of auxins either alone or in combination, 2, 4-D at a concentration of 0.5 mg/l was found to be most effective in callus production in a time period of 30 days. Callus derived from rhizome explants differentiated into shoots on MS medium supplemented with different concentrations of auxins (IAA, IBA) and (BAP). Maximum number of shoots (2.8±0.2) was regenerated on MS medium supplemented with BAP (5.0 mg/l) and IAA (2.0 mg/l) after 16 days.
Climate change is one of the main drivers for species redistribution and biodiversity loss, espec... more Climate change is one of the main drivers for species redistribution and biodiversity loss, especially for endemic and medicinally important plant species with a restricted distributional range. For that reason, it is vital to comprehend "how" and "where" priority medicinal and aromatic plants (MAPs) might be effectively used to address conservation-related issues under rapid climate change. In the current study, we used an ensemble modelling approach to investigate the present and future potential distribution coupled with the range dynamics of the Aquilegia fragrans-a medicinally important endangered plant species in the entire spectrum of the Himalayan biodiversity hotspot. The ndings of the current study revealed that, under current climatic conditions, the northwest states of India (Jammu and Kashmir, Himachal Pradesh, and the northern part of Uttarakhand); the eastern and southern parts of Pakistan Himalaya have highly suitable and optimal climatic conditions for the growth of A. fragrans. The ensemble model exhibited high forecast accuracy, with temperature seasonality (BIO4) and precipitation seasonality (BIO15) as the main climatic variables responsible for the distribution in the biodiversity hotspot. Furthermore, the study predicted that future climate change scenarios will diminish habitat suitability for the species by-46.922% under RCP4.5 2050 and − 55.052% under RCP4.5 2070. Likewise, under RCP8.5 the habitat suitability will decrease by-51.760% in 2050 and-94.351% in 2070. The current study also revealed that the western Himalayan area will show the most habitat loss. Some regions that are currently unsuitable such as the northern Himalayan regions of Pakistan will become more suitable under climate change scenarios. Hopefully, the current approach will provide a robust technique and showcases a model with learnings for predicting cultivation hotspots and devising scienti cally sound conservation plans for this endangered medicinal plant in the Himalayan biodiversity hotspot.
Journal of Pharmacognosy and Phytotherapy, Oct 31, 2013
Genus Origanum is important medicinally as it has antimicrobial, antifungal, antioxidant, antibac... more Genus Origanum is important medicinally as it has antimicrobial, antifungal, antioxidant, antibacterial, antithrombin, antimutagenic, angiogenic, antiparasetic and antihyperglycaemic activities. Phytochemical investigations of the species of this genus have resulted in the extraction of a number of important bioactive compounds. This emphasizes on the need of extensive study for reporting the additional information on the medicinal importance of other unattended species of genus Origanum.
Orchidaceae is a monocot family of herbaceous perennials that include terrestrial, saprophytic, l... more Orchidaceae is a monocot family of herbaceous perennials that include terrestrial, saprophytic, lithophytic and epiphytic species. It is one of the largest families among angiosperms with 800 genera and about 25, 000 species, distributed worldwide. Orchids comprise the highly evolved and economically important plants bearing the most beautiful and attractive flowers in the plant kingdom. From last 120 years a number of plant explorers have reported the occurrence of orchids from Kashmir Himalaya. In the present study different habitats of Kashmir Himalaya were evaluated for the occurrence of orchids. Ten species (Cephalanthera
Aconitum chasmanthum Stapf ex Holmes, an essential and critically endangered medicinal plant from... more Aconitum chasmanthum Stapf ex Holmes, an essential and critically endangered medicinal plant from Kashmir Himalayas, was studied for its antioxidant and antifungal properties. The shade-dried powdered rhizome was extracted sequentially with hexane, ethyl acetate, and methanol. These subsequent fractions were evaluated for total phenolic content (TPC); total flavonoid content (TFC); antioxidant assays, such as 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH); ferric-reducing antioxidant power (FRAP); superoxide radical scavenging (SOR); hydroxyl radical scavenging (OH) and antifungal activity using the poisoned food technique. Highest TPC (5.26 ± 0.01 mg/g) and TFC (2.92 ± 0.04 mg/g) were reported from methanolic extracts. The highest values of radical scavenging activities were also observed in methanolic extracts with IC50 values of 163.71 ± 2.69 μg/mL in DPPH, 173.69 ± 4.91 μg/mL in SOR and 159.64 ± 2.43 μg/mL in OH. The chemical profile of ethyl acetate extract was tested using HR-LCMS. M...
The Journal of Plant Science Research, Feb 10, 2023
Rhododendron campanulatum D.Don belongs to family Ericaceae, is an ornamental and medicinal plant... more Rhododendron campanulatum D.Don belongs to family Ericaceae, is an ornamental and medicinal plant which is commonly known as ‘Nichnai’ in Kashmir. During the present study, in vitro propagation strategies were used for micropropagation of Rhododendron campanulatum. Seeds of R. campanulatum were inoculated on Anderson Rhododendron basal medium (ARM) and also ARM supplemented with various auxins and cytokinins in different combinations and concentrations. Seed germination was observed on Anderson Rhododendron basal medium (ARM) and ARM + BAP (3mg/l) within 35.34 and 30.42 days of culture respectively. Seed germination was also observed on basal WPM as well as on WPM +2,4-D (4mg/l), WPM+BAP (1mg/l), WPM+BAP (2mg/l) respectively. In addition, callus production as well as shoot formation was observed on ARM supplemented with 2,4-D (3 and 4 mg/l) within 56.41 and 70.16 days respectively. Callus obtained from seed explants was subcultured on WPM supplemented with different cytokinins having different combinations. Among different cytokinin-cytokinin combinations used, WPM+2ip(2mg/l)+TDZ (4mg/l) induced maximum mean number 4.6±0.3 of shoots. The in vitro raised plantlets were acclimatized using cocopeat & perlite having different ratios as well as in garden soil under greenhouse conditions.
Journal of scientific and innovative research, Dec 25, 2014
Bunium persicum, family Apiaceae is a native of west Asia and has a limited distribution. During ... more Bunium persicum, family Apiaceae is a native of west Asia and has a limited distribution. During the present study an efficient in vitro protocol has been standardized viz, callus production from cotyledon, hypocotyl and nodal explants. Callus was obtained from these explants on MS medium fortified with different growth regulators both auxins (IAA, IBA, NAA, 2,4-D) and cytokinins (BAP and Kn). From cotyledon explants best callus production was achieved on MS medium supplemented with BAP (2 mg/l) +IAA (2 mg/l) within 10 days. For hypocotyls explants MS medium fortified with BAP (2 mg/l) resulted in best callus production within 24 days. MS medium supplemented with BAP (1 mg/l) +IAA (3 mg/l) proved to be most effective in callus production from nodal explants within 19 days.
Journal of scientific and innovative research, Oct 25, 2014
Rheum webbianum Royle is an important medicinal plant of family Polygonaceae growing in Kashmir H... more Rheum webbianum Royle is an important medicinal plant of family Polygonaceae growing in Kashmir Himalaya. It possesses hepatoprotective, spasmolytic, anticholesterolaemic, antitumor, antiseptic, antifungal, anti-microbial, anti-Parkinson's, anti-proliferative, immuno-enhancing, antiviral and antioxidant properties. During the present study an attempt has been made to standardize a protocol for large scale propagation of this threatened Himalayan plant species using rhizome cuttings as explants. Callus was obtained when the rhizome explants were inoculated on MS medium containing different concentrations of auxins either alone or in combination, 2, 4-D at a concentration of 0.5 mg/l was found to be most effective in callus production in a time period of 30 days. Callus derived from rhizome explants differentiated into shoots on MS medium supplemented with different concentrations of auxins (IAA, IBA) and (BAP). Maximum number of shoots (2.8±0.2) was regenerated on MS medium supplemented with BAP (5.0 mg/l) and IAA (2.0 mg/l) after 16 days.
Climate change is one of the main drivers for species redistribution and biodiversity loss, espec... more Climate change is one of the main drivers for species redistribution and biodiversity loss, especially for endemic and medicinally important plant species with a restricted distributional range. For that reason, it is vital to comprehend "how" and "where" priority medicinal and aromatic plants (MAPs) might be effectively used to address conservation-related issues under rapid climate change. In the current study, we used an ensemble modelling approach to investigate the present and future potential distribution coupled with the range dynamics of the Aquilegia fragrans-a medicinally important endangered plant species in the entire spectrum of the Himalayan biodiversity hotspot. The ndings of the current study revealed that, under current climatic conditions, the northwest states of India (Jammu and Kashmir, Himachal Pradesh, and the northern part of Uttarakhand); the eastern and southern parts of Pakistan Himalaya have highly suitable and optimal climatic conditions for the growth of A. fragrans. The ensemble model exhibited high forecast accuracy, with temperature seasonality (BIO4) and precipitation seasonality (BIO15) as the main climatic variables responsible for the distribution in the biodiversity hotspot. Furthermore, the study predicted that future climate change scenarios will diminish habitat suitability for the species by-46.922% under RCP4.5 2050 and − 55.052% under RCP4.5 2070. Likewise, under RCP8.5 the habitat suitability will decrease by-51.760% in 2050 and-94.351% in 2070. The current study also revealed that the western Himalayan area will show the most habitat loss. Some regions that are currently unsuitable such as the northern Himalayan regions of Pakistan will become more suitable under climate change scenarios. Hopefully, the current approach will provide a robust technique and showcases a model with learnings for predicting cultivation hotspots and devising scienti cally sound conservation plans for this endangered medicinal plant in the Himalayan biodiversity hotspot.
Journal of Pharmacognosy and Phytotherapy, Oct 31, 2013
Genus Origanum is important medicinally as it has antimicrobial, antifungal, antioxidant, antibac... more Genus Origanum is important medicinally as it has antimicrobial, antifungal, antioxidant, antibacterial, antithrombin, antimutagenic, angiogenic, antiparasetic and antihyperglycaemic activities. Phytochemical investigations of the species of this genus have resulted in the extraction of a number of important bioactive compounds. This emphasizes on the need of extensive study for reporting the additional information on the medicinal importance of other unattended species of genus Origanum.
Orchidaceae is a monocot family of herbaceous perennials that include terrestrial, saprophytic, l... more Orchidaceae is a monocot family of herbaceous perennials that include terrestrial, saprophytic, lithophytic and epiphytic species. It is one of the largest families among angiosperms with 800 genera and about 25, 000 species, distributed worldwide. Orchids comprise the highly evolved and economically important plants bearing the most beautiful and attractive flowers in the plant kingdom. From last 120 years a number of plant explorers have reported the occurrence of orchids from Kashmir Himalaya. In the present study different habitats of Kashmir Himalaya were evaluated for the occurrence of orchids. Ten species (Cephalanthera
Aconitum chasmanthum Stapf ex Holmes, an essential and critically endangered medicinal plant from... more Aconitum chasmanthum Stapf ex Holmes, an essential and critically endangered medicinal plant from Kashmir Himalayas, was studied for its antioxidant and antifungal properties. The shade-dried powdered rhizome was extracted sequentially with hexane, ethyl acetate, and methanol. These subsequent fractions were evaluated for total phenolic content (TPC); total flavonoid content (TFC); antioxidant assays, such as 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH); ferric-reducing antioxidant power (FRAP); superoxide radical scavenging (SOR); hydroxyl radical scavenging (OH) and antifungal activity using the poisoned food technique. Highest TPC (5.26 ± 0.01 mg/g) and TFC (2.92 ± 0.04 mg/g) were reported from methanolic extracts. The highest values of radical scavenging activities were also observed in methanolic extracts with IC50 values of 163.71 ± 2.69 μg/mL in DPPH, 173.69 ± 4.91 μg/mL in SOR and 159.64 ± 2.43 μg/mL in OH. The chemical profile of ethyl acetate extract was tested using HR-LCMS. M...
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