Papers by Shafquat Yasmin
Pak. J. Bot, 2011
Three sugarcane clones viz., NIA-98, NIA-0819 and BL4 were used for induction of genetic variabil... more Three sugarcane clones viz., NIA-98, NIA-0819 and BL4 were used for induction of genetic variability through In-vitro mutagenesis. Apical meristametic region was used for callus induction on 4mg/l 2,4-D. Actively growing callus was treated with four different doses of gamma rays (10Gy, 20Gy, 30Gy and 40Gy). Maximum callus, proliferation and plantlets regeneration growth was observed in control and minimum at 40Gy. Maximum chlorophyll mutation frequency was recorded in 30 and 40 Gy. Maximum number of roots was observed in control followed by 10Gy. The maximum root length (10.3cm) was obtained in control at 2 mg/l IBA. The maximum number of tiller in the irradiated population was observed in 10Gy. The treatments 30Gy and 40Gy exhibited negative impact on the tillering potential of the plant.
Twelve sugarcane clones were used in this study. The analysis of variance revealed significant di... more Twelve sugarcane clones were used in this study. The analysis of variance revealed significant differences among genotypes for all the plant characters. Highest cane yield (144 t/ha) was recorded in clone NIA82-1026P5 and highest Pol % (20.82) and CCS % (16.45) were observed in CP84-1198. Correlation coefficient results indicated that cane yield was positively correlated with cane girth, weight per stool, sugar yield, tiller numbers and purity % whereas Pol % and CCS % showed negative correlation with cane yield. Sugar yield showed non significant correlation with cane girth.
Callus was induced on immature embryos of wheat (Triticum aestivum L.) cvs. Sarsabz, Khirman and ... more Callus was induced on immature embryos of wheat (Triticum aestivum L.) cvs. Sarsabz, Khirman and Soghat-90. The application of different phytohormone concentrations yielded differential behaviour of genotypes for callus induction, mainly due to the genotype and media interaction. Variety Khirman produced highest callus on media (M1). Callus proliferation was also influenced by genotype and media interaction. Best callus proliferation was observed in Khirman on media (M4). Data revealed that Soghat-90 showed weakest interaction with present media compositions used in this study. Similarly regeneration potential was also subjective to media and genotype interaction. Data on chlorophyll mutants were also collected to study the frequency of genetic variation produced by each variety due to media effect. Green plantlets were transplanted in the field after weaning for evaluation of genetic variability.
Pakistan Journal of Botany
Three sugarcane clones viz., NIA-98, NIA-0819 and BL4 were used for induction of genetic variabil... more Three sugarcane clones viz., NIA-98, NIA-0819 and BL4 were used for induction of genetic variability through In-vitro mutagenesis. Apical meristametic region was used for callus induction on 4mg/l 2,4-D. Actively growing callus was treated with four different doses of gamma rays (10Gy, 20Gy, 30Gy and 40Gy). Maximum callus, proliferation and plantlets regeneration growth was observed in control and minimum at 40Gy. Maximum chlorophyll mutation frequency was recorded in 30 and 40 Gy. Maximum number of roots was observed in control followed by 10Gy. The maximum root length (10.3cm) was obtained in control at 2 mg/l IBA. The maximum number of tiller in the irradiated population was observed in 10Gy. The treatments 30Gy and 40Gy exhibited negative impact on the tillering potential of the plant.
Pakistan Journal of Botany, 2010
Pakistan Journal of Botany
Twelve sugarcane clones were used in this study. The analysis of variance revealed significant di... more Twelve sugarcane clones were used in this study. The analysis of variance revealed significant differences among genotypes for all the plant characters. Highest cane yield (144 t/ha) was recorded in clone NIA82-1026P5 and highest Pol % (20.82) and CCS % (16.45) were observed in CP84-1198. Correlation coefficient results indicated that cane yield was positively correlated with cane girth, weight per stool, sugar yield, tiller numbers and purity % whereas Pol % and CCS % showed negative correlation with cane yield. Sugar yield showed non significant correlation with cane girth.
Pakistan Journal of Botany
Ten elite sugarcane clones were tested for genetic diversity through RAPD, sucrose synthase activ... more Ten elite sugarcane clones were tested for genetic diversity through RAPD, sucrose synthase activity was determined via TRAP and drought tolerance was examined with the help of STS techniques / field trial. RAPD study revealed that genetically most similar genotypes were Thatta-10 and AEC82-223 (80.4%) and most dissimilar genotypes were AEC71-2011 and NIA-2004 (49.8%). On the basis of dendrogram, the varieties could be divided into four clusters (A to D). Variety AEC82-223 produced a specific allele of 311bp with primer B-02. Primer sucrose synthase amplified three alleles which were polymorphic and allelic size were 561, 327 and 222bp. Of 10, seven varieties tagged the specific gene responsible for drought tolerance in the genome. L116 containing a different allele of 912bp amplified with DREB-2 showed the specificity of the variety. Maximum sugar recovery % (14.82) and cane yield (t/ha) (156 t/ha) were recorded in AEC81-0819.
Pak. J. Bot, 2011
Three sugarcane (Saccharum sp. Hybrid) varieties viz., AEC82-1026, NIA-98 and BL4 were used in th... more Three sugarcane (Saccharum sp. Hybrid) varieties viz., AEC82-1026, NIA-98 and BL4 were used in this study in order to compare their response to different concentration of phytohormones (Dicamba 2,4D and Picloram). Best callus induction was observed using dicamba ...
The rapid multiplication of early, mid and late maturing sugarcane clones were obtained under dif... more The rapid multiplication of early, mid and late maturing sugarcane clones were obtained under different concentrations of commercial sugar and plant growth regulators in this study. Late maturing sugarcane clone AEC82-223 did not produce any auxillary shoot at 6% commercial sugar concentration. In contrast multiplication rate was higher in early and mid maturing sugarcane clones NIA-2004, BL4 and NIA-98, respectively. Apical meristems were cultured on modified MS medium containing different concentrations of auxins and cytokinins. An optimal multiplication was observed on M4 (1.0 mg/1 IAA + 1.0 mg/1 BAP + 0.1mg/l Kinetin) and M5 (0.l mg/1 kinetin + 1.5mg/l BAP + 1.5mg/l IAA). Maximum numbers of shoot were observed in BL4 followed by NIA-2004 when 1.0 mg/l of BAP and IAA were applied with low concentration of kinetin (0.1 mg/l). Shoot elongation and multiplication was improved on media devoid of BAP (MS+ 2 mg/1 kinetin + 2 mg/1 IBA + 2 mg/1 IAA). Best rooting was observed on media containing MS+ 1 mg/1 IBA + 6% sucrose.
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Papers by Shafquat Yasmin