Papers by Teruyoshi Yanagita
Lipids in Health and Disease, 2011
Background: Soy protein and soy peptides have attracted considerable attention because of their p... more Background: Soy protein and soy peptides have attracted considerable attention because of their potentially beneficial biological properties, including antihypertensive, anticarcinogenic, and hypolipidemic effects. Although soy protein isolate contains several bioactive peptides that have distinct physiological activities in lipid metabolism, it is not clear which peptide sequences are responsible for the triglyceride (TG)-lowering effects. In the present study, we investigated the effects of soy protein-derived peptides on lipid metabolism, especially TG metabolism, in HepG2 cells and obese Otsuka Long-Evans Tokushima fatty (OLETF) rats. Results: In the first experiment, we found that soy crude peptide (SCP)-LD3, which was prepared by hydrolyze of soy protein isolate with endo-type protease, showed hypolipidemic effects in HepG2 cells and OLETF rats. In the second experiment, we found that hydrophilic fraction, separated from SCP-LD3 with hydrophobic synthetic absorbent, revealed lipid-lowering effects in HepG2 cells and OLETF rats. In the third experiment, we found that Fraction-C (Frc-C) peptides, fractionated from hydrophilic peptides by gel permeation chromatography-high performance liquid chromatography, significantly reduced TG synthesis and apolipoprotein B (apoB) secretion in HepG2 cells. In the fourth experiment, we found that the fraction with 0.1% trifluoroacetic acid, isolated from Frc-C peptides by octadecylsilyl column chromatography, showed hypolipidemic effects in HepG2 cells. In the final experiment, we found that 3 di-peptides, Lys-Ala, Val-Lys, and Ser-Tyr, reduced TG synthesis, and Ser-Tyr additionally reduced apoB secretion in HepG2 cells.
The Journal of Nutritional Biochemistry, 2006
Journal of Oleo Science, 2020
Introduction The metabolic syndrome is known to increase cardiovascular morbidity and mortality, ... more Introduction The metabolic syndrome is known to increase cardiovascular morbidity and mortality, and central obesity is the key component of its development 1 3. Although the pathogenesis of metabolic syndrome is complicated, lipid abnormality is proposed as a feature of metabolic syndrome 1 3. Many studies suggested that plant proteins could be important modulators of the risks associated with this syndrome 4, 5. For example, several studies have shown that dietary soy protein reduces cholesterol and triglyceride TG levels in animals and humans 4 8. Moreover soy β-conglycinin βCG , a soybean storage protein, has been reported to exert physiological effects such as promoting lipid lowering effects and preventing obesity in several animal models 9 13. However, physiological functions of βCG in non-high-fat or non-high-cholesterol diet-fed rodents have not been fully evalutated, and almost all of previous studies have been conducted with diets in which all dietary protein was replaced by βCG even though its es
JOURNAL OF THE BREWING SOCIETY OF JAPAN
Dietary glycosylceramide has several benefits including reducing transepidermal water loss and im... more Dietary glycosylceramide has several benefits including reducing transepidermal water loss and improving intestinal microbial flora. We have elucidated that koji, the base for Japanese traditional cuisines, contains abundant glycosylceramide. To investigate the content of koji glycosylceramide contained in Japanese foods made with koji, a quantitative method of koji glycosylceramide using TLC-orcinol sulphate staining was investigated. First, the existence of ions having m/z of 4,8-sphingadienine and 9-methyl-4,8sphingadienine, major molecules comprising koji glycosylceramide, was confirmed by ESI-MS. Using an OPA derivatization-HPLC method, the validity of a TLC-orcinol sulphate staining method as a glyocsylceramide-quantitative method was verified. Using a TLC-orcinol sulphate staining method, it was elucidated that unfiltered sake, amazake, and salted koji contained abundant amounts of koji glygosylceramide of up to 4.15 mg glycosylceramide per serving. From the information elucidated in this study, it was estimated that a Japanese meal containing koji-based foods could contain 8.69 mg of glycosylceramide. This is the first study to elucidate the content of glycosylceramide contained in Japanese foods made with koji.
International Journal of Molecular Sciences
Glucosylceramide is present in many foods, such as crops and fermented foods. Most glucosylcerami... more Glucosylceramide is present in many foods, such as crops and fermented foods. Most glucosylceramides are not degraded or absorbed in the small intestine and pass through the large intestine. Glucosylceramide exerts versatile effects on colon tumorigenesis, skin moisture, cholesterol metabolism and improvement of intestinal microbes in vivo. However, the mechanism of action has not yet been fully elucidated. To gain insight into the effect of glucosylceramide on intestinal microbes, glucosylceramide was anaerobically incubated with the dominant intestinal microbe, Blautia coccoides, and model intestinal microbes. The metabolites of the cultured broth supplemented with glucosylceramide were significantly different from those of broth not treated with glucosylceramide. The number of Gram-positive bacteria was significantly increased upon the addition of glucosylceramide compared to that in the control. Glucosylceramide endows intestinal microbes with tolerance to secondary bile acid. T...
Metabolites, 2021
Dietary sterols are catabolized into various substances in the intestinal tract. Dietary 3-oxo de... more Dietary sterols are catabolized into various substances in the intestinal tract. Dietary 3-oxo derivatives of cholesterol and plant sterols (e.g., cholest-4-en-3-one and campest-5-en-3-one) have been shown to have anti-obesity effects. In this study, we tested whether feeding cholest-5-en-3-one (5-cholestenone), a cholesterol metabolite, to db/db mice protects them from obesity-associated metabolic disorders. In db/db mice, dietary 5-cholestenone significantly alleviated hepatomegaly and elevated serum triglyceride levels; however, the effect was not sufficient to improve hepatic steatosis and obesity. On the other hand, hyperglycemia and severe hyperinsulinemia in control db/db mice were markedly attenuated in 5-cholestenone-fed db/db mice. The production of inflammatory cytokines, such as monocyte chemoattractant protein-1, interleukin-6, and tumor necrosis factor-alpha (TNFα), was decreased, suggesting that the suppressive actions of 5-cholestenone were attributable to the allevi...
Journal of the agricultural chemical society of Japan, 1973
Agricultural and Biological Chemistry, 1976
Journal of nutritional science and vitaminology, 2015
Metabolic syndrome is a cluster of metabolic disorders that contribute to increased cardiovascula... more Metabolic syndrome is a cluster of metabolic disorders that contribute to increased cardiovascular morbidity and mortality. Although the pathogenesis of metabolic syndrome is complicated, dietary lipids have been recognized as contributory factors in the development and the prevention of cardiovascular risk clustering. We investigated the physiological functions and molecular actions of functional lipids, especially omega3-polyunsaturated fatty acid (PUFA)-containing lipids, in the development of metabolic syndrome using obese model animals. Feeding of omega3-PUFA-containing lipids, such as eicosapentaenoic acid, docosapentaenoic acid, docosahexaenoic acid, tetracosahexaenoic acid, and omega3-phosphatidylcholine, alleviated hepatic lipid accumulation through the suppression of lipogenic gene expression in the liver. Additionally, dietary omega3-PUFA-containing lipids increased serum adiponectin levels in obese animal models. Their molecular actions in the prevention and alleviation ...
Fermentation, 2016
Koji, rice fermented with Aspergillus, is used for saccharification of starch contained in crops ... more Koji, rice fermented with Aspergillus, is used for saccharification of starch contained in crops during the manufacturing of many of Japanese traditional foods and drinks. Japanese people have long eaten koji, and many beneficial substances have been reported to be contained in koji. However, there has been no report on the existence or content of galactosylceramide in koji. To address this issue, we analyzed the chemical composition of the sugar moiety of monohexosylceramide contained in koji, and elucidate that 30.3% of yellow koji is galactosylceramide, 69.7% of that is glucosylceramide, 19.2% of white koji is galactosylceramide, and 80.8% of that is glucosylceramide. This is the first report of the existence and content of galactosylceramide in koji.
Bioscience, biotechnology, and biochemistry, Jan 18, 2016
We investigated whether fatty liver preceded insulin resistance or vice versa using a long-term o... more We investigated whether fatty liver preceded insulin resistance or vice versa using a long-term orotic acid (OA)-induced nonalcoholic fatty liver disease (NAFLD) model without the confounding effects of obesity and hyperlipidemia and explored the role of the liver in insulin resistance. Male Wistar rats were fed with or without OA supplementation for 30, 60, and 90 days. The NAFLD group showed increased liver lipid at 30, 60, and 90 days; glucose intolerance was noted at 60 and 90 days. Furthermore, partial liver proteins and gene expressions related to upstream signaling of insulin were decreased. However, the liver glycogen content was elevated, and gluconeogenesis genes expressions were obviously decreased at 90 days. The occurrence of fatty liver preceded insulin resistance in OA-induced NAFLD without the interference of obesity and hyperlipidemia, and hepatic insulin resistance may not play a conclusive role in insulin resistance in this model.
Agricultural and Biological Chemistry, 1986
Administration of di-(2-ethylhexyl)phthalate (DEHP), the most commonlyused plasticizer for polyvi... more Administration of di-(2-ethylhexyl)phthalate (DEHP), the most commonlyused plasticizer for polyvinylchloride products, to rats had been reported1~5) to cause the alteration of hepatic lipid metabolism and hepatic peroxisomal proliferation, which was accompanied by an increased in the peroxisome-associated enzymes catalase
Agricultural and Biological Chemistry, 1984
International Food Research Journal
This study was conducted to ascertain the cytotoxicity effect of oil palm (Elaeis guineensis) ker... more This study was conducted to ascertain the cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates (OPKHs) produced from its protein isolate. A modified microplate titer WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay was used to investigate the cytotoxicity of hydrolysates produced from protease and pepsin-pancreatin hydrolysis at various concentrations (0.1, 1, 10, 100 μg/ml and 1 mg/ml) using HepG2 cell model. Additionally, peptide stimulation test using OPKHs at 1 mg/ml was carried out to investigate whether OPKHs could serve as growth factor for HepG2 cells other than affecting its viability. As a result, oleic acid appeared to normalize the WST-1 readings of HepG2 cells treated with both hydrolysates at 1 mg/ml. The presence of amino acids in OPKHs could stimulate the growth and prolongs the viability of HepG2 cells. Both OPKHs were non-cytotoxic to HepG2 cells at all tested concentrations even at high concentrations...
Journal of Oleo Science, 2002
Hydrogenation is the most frequently used method for processing of fats and oils and during which... more Hydrogenation is the most frequently used method for processing of fats and oils and during which, some double bonds of fatty acids are saturated while cis double bonds in some cases may be isomerized to the trans form (1). Trans fatty acid leads to physical and chemical properties not seen in natural oils (2,3). Partially hydrogenated fats and oils containing trans fatty acid are thus widely used as edible fats and oils in foods. Considerable consumption of trans fatty acid may increase low-density lipoprotein cholesterol (LDL-C)
The Japanese Journal of Nutrition and Dietetics, 1994
Bioscience, biotechnology, and biochemistry, 2014
Many animal studies on improvement of lipid metabolism, using dietary components, fast the animal... more Many animal studies on improvement of lipid metabolism, using dietary components, fast the animals on the final day of the feeding. Although fasting has a significant impact on lipid metabolism, its time-dependent influence is not fully understood. We examined the effects of several fasting times on lipid metabolism. Rats fed with a semisynthetic diet for 2 wk were killed after 0 (9:00 am), 6 (7:00 am-1:00 pm), 9 (0:00 am-9:00 am), and 13 h (8:00 pm-9:00 am) of fasting. Compared to the 0 h group, marked reduction of liver weight and hepatic triacylglycerol content was observed in the 9 and 13 h groups. Activities of hepatic enzymes involved in fatty acid synthesis gradually decreased during fasting. In contrast, drastic time-dependent reduction of gene expression, of the enzymes, was observed. Expression of carnitine palmitoyltransferase mRNA was higher in the fasting groups than in the 0 h group. Our study showed that fasting has a significant impact on several parameters related t...
Journal of Oleo Science, 2001
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Papers by Teruyoshi Yanagita