Papers by Tasanee Panichakul
PROTEOMICS, 2004
Cholangiocarcinoma (CCA), a malignant tumor derived from bile duct epithelium, occurs with a high... more Cholangiocarcinoma (CCA), a malignant tumor derived from bile duct epithelium, occurs with a higher incidence in tropical countries, such as Thailand. Distinguishing CCA from hepatocellular carcinoma (HCC) of the liver often requires the use of histochemistry, so molecular markers for diagnosis and prognosis are still required. In this study, the two‐dimensional (2‐D) protein map of a Thai human bile duct epithelial carcinoma cell line (HuCCA‐1) has been compared to human hepatocellular carcinoma cell lines (HepG2 and HCC‐S102) and a human breast epithelial cancer cell line (MCF‐7). Our results show that HuCCA‐1 expressed a unique pattern of proteins. Forty‐three major proteins were identified by matching to the map of MCF‐7, and by matrix assisted laser desorption/ionization‐time of flight‐mass spectrometry (MALDI‐TOF‐MS) and electrospray ionization‐tandem MS (ESI‐MS/MS). Cytokeratins CK8 and CK18 were overexpressed in both HuCCA‐1 and HCC, while CK7 and CK19 were only expressed in...
Reports of severe anemia due to P. vivax infection increase [1]. P. vivax is considered to infect... more Reports of severe anemia due to P. vivax infection increase [1]. P. vivax is considered to infect reticulocytes and parasitemia is generally low, this suggests that in addition to the simple destruction of infected red cells there is another mechanism to induce anemia in vivax. A report of vivax malaria in bone marrow from severely anemia patients exhibited dyserythropoiesis [2]. Our study demonstrated that P. vivax could infect erythroblasts and erythroblast loss was at least partially attributed to direct killing by parasite invasion [3]. However, the mechanism involving in induction of anemia in vivax malaria is still unclear. Here, hematopoietic stem cells (HSCs)/CD34 + cells from normal human cord blood were subjected to study the suppression of erythropoiesis by P. vivax infection. Erythroid cells derived from HSCs were cultured in serum-free medium supplemented with growth factors and cytokines. Intact or lysed P. vivax-infected erythrocytes (PV-IE) isolated from patient bloo...
Journal of Medical Microbiology, 2000
A monoclonal antibody-based latex agglutination (MAb-LA) test was employed for the rapid identi®c... more A monoclonal antibody-based latex agglutination (MAb-LA) test was employed for the rapid identi®cation of Burkholderia pseudomallei in blood culture¯uid from patients with community-acquired septicaemia. These patients were admitted to 12 hospitals in the northeastern part of Thailand which is a region known to be endemic for melioidosis. Blood samples were collected and immediately added to the blood culture bottles which were incubated in either automated (®ve hospitals) or manual (seven hospitals) culture systems. Of a total of 1369 culture-positive specimens, 204 specimens were culture-positive for B. pseudomallei. Of those, 194 (95%) were positive by MAb-LA and the type of blood culture system did not affect positivity rates. The performance of the MAb-LA test on these specimens was highly satisfactory compared with culture detection and con®rmation by biochemical test, with 95.1% sensitivity, 99.7% speci®city and 98.8% and 99.2% for positive and negative predictive values, respectively. The method described is highly reproducible, simple to perform even by inexperienced laboratory personnel and does not require expensive or elaborate equipment.
Pharmaceuticals
Artocarpus lakoocha (Al) and Glycyrrhiza glabra (Gg) extracts have been reported to show tyrosina... more Artocarpus lakoocha (Al) and Glycyrrhiza glabra (Gg) extracts have been reported to show tyrosinase inhibitory activity and melanin pigment reduction. This is the first study to assess the combination of Al and Gg extracts in enhancing inhibition of tyrosinase and reduction of melanin pigments. Al and Gg extracted by maceration in 70% and 95% ethanol were analyzed for oxyresveratrol and glabridin using Ultra High Performance Liquid Chromatography. Extracts of Al and Gg singly and combinations of Al95 and Gg95 were tested for cytotoxicity, tyrosinase inhibitory activity, and reduction of melanin pigments in melanoma B16 cells. Al95 had higher antioxidant, tyrosinase inhibitory activity and reduced more melanin pigments in B16 cells compared to Al70, and exhibited higher levels of oxyresveratrol. Gg95 inhibited oxidative stress and mushroom tyrosinase better than Gg70, and exhibited higher levels of glabridin. Combinations of Al95 and Gg95 at various ratios (concentration of 0.1 mg/mL...
ScienceAsia, 2021
Rhyncholaeliocattleya Haw Yuan Beauty (Rlc. Haw Yuan Beauty) is a traditional orchid in tropical ... more Rhyncholaeliocattleya Haw Yuan Beauty (Rlc. Haw Yuan Beauty) is a traditional orchid in tropical and subtropical Asian regions, which has attracted attention for its fragrant flowers with diverse colors. This study aimed to investigate the effects of different extraction methods on the phenolic profile, total flavonoid and cyanidin-3-Oglucoside contents, and anti-aging properties of Rlc. Haw Yuan Beauty crude extracts. The crude extracts were obtained using solvent, ultrasonic, and supercritical carbon dioxide fluid extraction methods. The major phenolic compounds including gallic, chlorogenic, and caffeic acids were present in all the extracts. The extract obtained with solvent extraction showed the highest total flavonoid content, whereas that obtained with supercritical fluid extraction yielded the highest cyanidin-3-O-glucoside content. Additionally, the different extraction methods had a significant influence on the antioxidant, anti-elastase, and anti-collagenase activities (p < 0.05) of Rlc. Haw Yuan Beauty extracts. The crude extracts derived from the ultrasonic and supercritical carbon dioxide fluid extraction methods were not cytotoxic to human skin fibroblasts at any of the tested concentrations (31-500 µg/ml). Thus, the crude extracts from Rlc. Haw Yuan Beauty could be a safe and active ingredient in the production of skincare products in the cosmetic industry for human use.
Clinical hemorheology and microcirculation, 2004
A persistent proteinuria is commonly observed in nephrotic patient with focal segmental glomerulo... more A persistent proteinuria is commonly observed in nephrotic patient with focal segmental glomerulosclerosis (FSGS) under treatment with prednisolone+/-cyclophosphamide or with vasodilators (ACEI+AII receptor antagonist, calcium channel blocker and antiplatelet agent). Fourteen such patients with persistent proteinuria were subject to be treated with Ganoderma lucidum. Initial study revealed an enhanced endothelial cell cytotoxicity induced by patient's serum, and an altered immunocirculatory balance with predominant proinflammatory cytokine TNF alpha activity in the presence of defective anti-inflammatory cytokine interleukin-10. Treatment with Ganoderma lucidum suppressed endothelial cell cytotoxicity, restored immunocirculatory balance and successfully suppressed proteinuria in all of these 14 patients.
Anticancer research
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L) promotes apoptosis by bindi... more Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L) promotes apoptosis by binding to transmembrane receptors. It is known to induce apoptosis in a wide variety of cancer cells, but TRAIL-resistant cancers have also been documented. In this study, the relative resistance of human cholangiocarcinoma (CCA) cell lines against TRAIL-induced apoptosis is reported and the possible potential synergistic effect with triptolide, a diterpene triepoxide extracted from the Chinese herb Tripterygium wilfordii, in killing TRAIL-resistant CCA cells is investigated. Six human CCA cell lines were treated with various concentrations of TRAIL and the resistant cells were identified and subsequently tested for their sensitivity to a combination of TRAIL and triptolide. The susceptibility and resistance of the cells were based on analysis of cytotoxic and apoptotic induction and expression of anti-apoptotic factors (Mcl-1 and cFLIP). The treatment of TRAIL induced a dose-dependent decre...
Pharmaceuticals
Collagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phy... more Collagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phytochemical substances are desirable for the prevention of skin aging and the formation of wrinkles. Ipomoea pes-caprae (IPC) has been utilized for nutritional and therapeutic purposes, and its extract contains collagenase inhibitory activity while causing no cytotoxicity. The purpose of this study was to examine the impact of IPC extracts on cell proliferation and collagen production in human fibroblasts (CCD-986sk cells). IPC leaves were macerated in 70% and 95% ethanol and the chemical composition of the resulting extracts (IPC70 and IPC95) were determined using high performance liquid chromatography (HPLC). The bioactivity of IPC extracts was examined in CCD-986sk cells, including antioxidant capacity, inhibition of collagenase, effects on cell proliferation and collagen production, as well as wound healing using an in vitro scratch test. Changes in expression of collagen type I (COL1...
Journal of Translational Medicine, 2011
Background Hemoglobin E/β-thalassemia is particularly common in Southeast Asia and has variable s... more Background Hemoglobin E/β-thalassemia is particularly common in Southeast Asia and has variable symptoms ranging from mild to severe anemia. Previous investigations demonstrated the remarkable symptoms of β-thalassemia in terms of the acceleration of apoptotic cell death. Ineffective erythropoiesis has been studied in human hematopoietic stem cells, however the distinct apoptotic mechanism was unclear. Methods The phosphoproteome of bone marrow HSCs/CD34+ cells from HbE/β-thalassemic patients was analyzed using IMAC phosphoprotein isolation followed by LC-MS/MS detection. Decyder MS software was used to quantitate differentially expressed proteins in 3 patients and 2 normal donors. The differentially expressed proteins from HSCs/CD34+ cells were compared with HbE/β-thalassemia and normal HSCs. Results A significant change in abundance of 229 phosphoproteins was demonstrated. Importantly, the analysis of the candidate proteins revealed a high abundance of proteins that are commonly f...
Plasmodium vivax invasion of human erythrocytes inhibited by antibodies directed against the duff... more Plasmodium vivax invasion of human erythrocytes inhibited by antibodies directed against the duffy binding protein. PLoS Med 4(12): e337.
Journal of Public Health วารสารสาธารณสุขศาสตร์, Apr 6, 2010
Advances in Microbiology, 2015
EXCLI journal, 2015
Hematopoietic stem cells (HSC) from cord blood are potentially high sources for transplantation d... more Hematopoietic stem cells (HSC) from cord blood are potentially high sources for transplantation due to their low immunogenicity and the presence of the multipotent cells. These cells are capable of differentiating to produce various lineages of blood cells under specific conditions. We have enriched highly purified CD34(+) cells from cord blood, determined in vitro growth of the cells in culture systems in the absence (condition A) or presence of GM-CSF and G-CSF (condition B), and determined the profile of immune cells during the period of cultivation by using flow cytometry. PhytohemagglutininA (PHA) was used as a mitogen to stimulate T lymphocytes derived from hematopoietic stem cells. GM-CSF and G-CSF prolonged the survival of the growing cells and also maintained expansion of cells in blastic stage. By day 12 of cultivation, when cell numbers peaked, various types of immune cells had appeared (CD14(+) cells, CD40(+)HLA-DR(+) cells, CD3(+)CD56(+) cells, CD19(+) cells, CD3(+)CD4(...
A dysfunctioning glomerular endothelium was demonstrated in chronic kidney disease (CKD) patients... more A dysfunctioning glomerular endothelium was demonstrated in chronic kidney disease (CKD) patients by means of in vitro endothelial cell cytotoxicity test and of in vivo intrarenal hemodynamic study. An enhanced endothelial cell cytotoxicity in CKD patients was 26.5 +/- 12% as compared to 0.4 +/- 1% of control. An altered intrarenal hemodynamics revealed 1) a reduction in renal plasma flow, 190 +/- 67 mL/min/1.73 m2 versus control 595 +/- 45 mL/min/1.73 m2, and in peritubular capillary flow, 149 +/- 55 mL/min/1.73 m2 versus control 479 +/- 46 mL/min/1.73 m2, 2) an elevated intraglomerular hydrostatic pressure, 55 +/- 2 mmHg versus control 51 mmHg, elevated afferent arteriolar resistance, 13184 dyne x s x cm(-5) versus control 2443 +/- 154 dyne x s x cm(5), and elevated efferent arteriolar resistance, 13591 +/- 7591 dyne x s x cm(-5) versus control 3062 +/- 177 dyne x s x cm(-5). Both enhanced endothelial cell cytotoxicity and altered intrarenal hemodynamics reflect glomerular endothelial dysfunction which is likely responsible for the renal disease progression in CKD.
Clinical Hemorheology and Microcirculation, 2003
Background: The underlying causes of severe malarial anaemia are multifactorial. In previously re... more Background: The underlying causes of severe malarial anaemia are multifactorial. In previously reports, Plasmodium vivax was found to be able to directly inhibited erythroid cell proliferation and differentiation. The molecular mechanisms underlying the suppression of erythropoiesis by P. vivax are remarkably complex and remain unclear. In this study, a phosphoproteomic approach was performed to dissect the molecular mechanism of phosphoprotein regulation, which is involved in the inhibitory effect of parasites on erythroid cell development.
Malaria Journal, 2015
The underlying causes of severe malarial anaemia are multifactorial. In previously reports, Plasm... more The underlying causes of severe malarial anaemia are multifactorial. In previously reports, Plasmodium vivax was found to be able to directly inhibited erythroid cell proliferation and differentiation. The molecular mechanisms underlying the suppression of erythropoiesis by P. vivax are remarkably complex and remain unclear. In this study, a phosphoproteomic approach was performed to dissect the molecular mechanism of phosphoprotein regulation, which is involved in the inhibitory effect of parasites on erythroid cell development. This study describes the first comparative phosphoproteome analysis of growing erythroid cells (gECs), derived from human haematopoietic stem cells, exposed to lysates of infected erythrocytes (IE)/uninfected erythrocytes (UE) for 24, 48 and 72 h. This study utilized IMAC phosphoprotein isolation directly coupled with LC MS/MS analysis. Lysed IE significantly inhibited gEC growth at 48 and 72 h and cell division resulting in the accumulation of cells in G0 phase. The relative levels of forty four phosphoproteins were determined from gECs exposed to IE/UE for 24-72 h and compared with the media control using the label-free quantitation technique. Interestingly, the levels of three phosphoproteins: ezrin, alpha actinin-1, and Rho kinase were significantly (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) altered. These proteins display interactions and are involved in the regulation of the cellular cytoskeleton. Particularly affected was ezrin (phosphorylated at Thr567), which is normally localized to gEC cell extension peripheral processes. Following exposure to IE, for 48-72 h, the ezrin signal intensity was weak or absent. This result suggests that phospho-ezrin is important for actin cytoskeleton regulation during erythroid cell growth and division. These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. A better understanding of the mechanisms of ineffective erythropoiesis may be beneficial in the development of therapeutic strategies to prevent severe malarial anaemia.
Clinical hemorheology and microcirculation, 2004
A persistent proteinuria is commonly observed in nephrotic patient with focal segmental glomerulo... more A persistent proteinuria is commonly observed in nephrotic patient with focal segmental glomerulosclerosis (FSGS) under treatment with prednisolone+/-cyclophosphamide or with vasodilators (ACEI+AII receptor antagonist, calcium channel blocker and antiplatelet agent). Fourteen such patients with persistent proteinuria were subject to be treated with Ganoderma lucidum. Initial study revealed an enhanced endothelial cell cytotoxicity induced by patient's serum, and an altered immunocirculatory balance with predominant proinflammatory cytokine TNF alpha activity in the presence of defective anti-inflammatory cytokine interleukin-10. Treatment with Ganoderma lucidum suppressed endothelial cell cytotoxicity, restored immunocirculatory balance and successfully suppressed proteinuria in all of these 14 patients.
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Papers by Tasanee Panichakul