Papers by Tania de Andrade Rodrigues
Brazilian Journal of Otorhinolaryngology, 2015
The most common pathogen in bacteria lpharyngotonsillitis is group A β-hemolytic streptococcus, a... more The most common pathogen in bacteria lpharyngotonsillitis is group A β-hemolytic streptococcus, although groups B, C, F,and G have also been associated with pharyngotonsillitis. To assess the levels of the cytokines TNF-α, IL-6,IL-4, and IL-10 in bacterial pharyngotonsillitis caused by group A and non-A (groups B, C, F and G) β-hemolytic streptococcus. The study was conducted at a pediatric emergency care unit. The sample comprised children (5-9 years old) with acute bacterial pharyngotonsillitis diagnosed between December of 2011 and May of 2012. The research involved collection of blood samples from the patients, enzyme-linked immunosorbent assay detection of TNF-α, IL-6,IL-4, and IL-10, and collection of two oropharyngeal swabs for bacterial isolation. Additionally, the medical history of the study participants was also collected. In the studied group (mean age: 5.93 years), higher pharyngotonsillitis incidence was observed in the female gender (64.76%). Higher incidence of tonsillar exudates was observed with groups A and C. No statistically significant differences in cytokine levels were observed among groups. However, the group A and the control group showed a difference in the IL-6 level (p=0.0016). The Groups A and C showed higher cytokine levels than the Groups B and control, suggesting similar immunological patterns.
Revista brasileira de cirurgia cardiovascular : órgão oficial da Sociedade Brasileira de Cirurgia Cardiovascular, 2012
Rheumatic fever is a highly prevalent disease in Brazil, and it poses a major public health probl... more Rheumatic fever is a highly prevalent disease in Brazil, and it poses a major public health problem. It is the leading cause of acquired heart disease in childhood and adolescence. The aim of this study was to evaluate the gene expression of ET-3 and its receptors, in replaced rheumatic mitral valves. We studied the gene expression of endothelin-3 (ET-3) and its receptors, endothelin receptor A and endothelin receptor B (ETr-A and ETr-B), in the rheumatic mitral valves of 17 patients who underwent valve replacement surgery. The samples also underwent a histological analysis. Our data showed that almost all patients, regardless of individual characteristics such as gender or age, expressed the endothelin receptor genes, but did not express the genes for ET-3. In quantitative analysis, the ETr-A/GAPDH mean ratio was 33.04 ± 18.09%; while the ETr-B/GAPDH mean ratio was 114.58 ± 42.30%. Regarding histopathological individual features, the frequency of fibrosis is 100%, 88.23% of mononuc...
Revista Brasileira de Cirurgia Cardiovascular, 2014
Objetivo: Avaliar o perfil imunológico e a expressão gênica de endotelina-1 em valvas mitrais de ... more Objetivo: Avaliar o perfil imunológico e a expressão gênica de endotelina-1 em valvas mitrais de pacientes com febre reumática, originados de um serviço de referência em cirurgia cardiovascular. Métodos: Este foi um estudo quantitativo, observacional e transversal. Trinta e cinco indivíduos (divididos em quatro grupos) participaram do estudo, 25 deles com doença cardíaca reumática crônica, além de 10 controles. A média de idade da amostra estudada foi de 34,5 anos. Dezessete (48,58%) dos indivíduos eram homens, e 18 (51,42%) eram mulheres. Foram medidas algumas citocinas inflamatórias (TNF-α, IL-4 e IL-10) e coletadas 10 valvas mitrais de pacientes que se submeteram a primeira troca valvar para determinação da expressão gênica de endotelina-1 pelo PCR real-time. Resultados: Entre os grupos estudados (pacientes e controles), observou-se diferença estatisticamente significante em rela-Rev Bras Cir Cardiovasc | Braz J Cardiovasc Surg Rev Bras Cir Cardiovasc 2014;29(1):25-30 Leão SC, et al. -IL-10 and ET-1 as biomarkers of rheumatic valve disease Rev Bras Cir Cardiovasc | Braz J Cardiovasc Surg Rev Bras Cir Cardiovasc 2014;29(1):25-30 Leão SC, et al. -IL-10 and ET-1 as biomarkers of rheumatic valve disease Rev Bras Cir Cardiovasc | Braz J Cardiovasc Surg Rev Bras Cir Cardiovasc 2014;29(1):25-30 Leão SC, et al. -IL-10 and ET-1 as biomarkers of rheumatic valve disease
Revista Brasileira de Cirurgia Cardiovascular, 2015
Rheumatic Fever represents a serious public health problem in developing countries, with thousand... more Rheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A. The aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves. Immunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples. ET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis. The strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.
Histopathology, 2012
... be graded and a comment added that if there is any residual gross lesion, a repeat biopsy ma... more ... be graded and a comment added that if there is any residual gross lesion, a repeat biopsy may be warranted and, if not, then close follow-up is recommended. There are 8 histologic variants of squamous cell carcinoma: conventional, verrucous, spindle cell, papillary, basaloid ...
Life Sciences, 2013
entered as independent predictors of aortic pulse wave velocity (PWV, a traditional index of arte... more entered as independent predictors of aortic pulse wave velocity (PWV, a traditional index of arterial stiffness) into the stepwise regression analysis of sex, antihypertensive medication use, body mass index, blood test results, resting blood pressure, daily physical activity and diastolic blood pressure and HR during the exercise (r 2 = 0.634, P b 0.001). In subgroup analyses according to average SBP during the exercises, aortic PWV was higher in the high SBP group than the low SBP group independent of resting SBP (P b 0.001). We concluded that SBP during resistance exercise is independently correlated with arterial stiffness. The following steps are to elucidate whether excessive blood pressure elevation during resistance exercise increases arterials stiffness by prospective study and to investigate its relationship to ET-1 system.
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Papers by Tania de Andrade Rodrigues