International Journal of Systematic and Evolutionary Microbiology, 2014
Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded... more Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average blast nucleotide identity (ANIb) of less than 85 % to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria ; (i) a clade representing Listeria monocytogenes , L. marthii , L. innocua , L. welshimeri , L. seeligeri and L. ivanovii , which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188T = DSM 26686T = LMG 28120T = BEI NR-42633T) and Listeria floridensis sp. nov. (type stra...
Salmonella accounts for approximately 50% of produce-associated outbreaks in the United States, s... more Salmonella accounts for approximately 50% of produce-associated outbreaks in the United States, several of which have been traced back to contamination in the produce production environment. To quantify Salmonella diversity and aid in identification of Salmonella contamination sources, we characterized Salmonella isolates from two geographically diverse produce-growing regions in the United States. Initially, we characterized the Salmonella serotype and subtype diversity associated with 1,677 samples collected from 33 produce farms in New York State (NYS). Among these 1,677 samples, 74 were Salmonella positive, yielding 80 unique isolates (from 147 total isolates), which represented 14 serovars and 23 different pulsed-field gel electrophoresis (PFGE) types. To explore regional Salmonella diversity associated with production environments, we collected a smaller set of samples (n = 65) from South Florida (SFL) production environments and compared the Salmonella diversity associated wi...
ABSTRACTProduce-related outbreaks have been traced back to the preharvest environment. A longitud... more ABSTRACTProduce-related outbreaks have been traced back to the preharvest environment. A longitudinal study was conducted on five farms in New York State to characterize the prevalence, persistence, and diversity of food-borne pathogens in fresh produce fields and to determine landscape and meteorological factors that predict their presence. Produce fields were sampled four times per year for 2 years. A total of 588 samples were analyzed forListeria monocytogenes,Salmonella, and Shiga toxin-producingEscherichia coli(STEC). The prevalence measures ofL. monocytogenes,Salmonella, and STEC were 15.0, 4.6, and 2.7%, respectively.L. monocytogenesandSalmonellawere detected more frequently in water samples, while STEC was detected with equal frequency across all sample types (soil, water, feces, and drag swabs).L. monocytogenes sigBgene allelic types 57, 58, and 61 andSalmonella entericaserovar Cerro were repeatedly isolated from water samples. Soil available water storage (AWS), temperatur...
Five new species of Listeria (L. floridensis sp. nov, L. aquatica sp. nov., L. cornellensis sp. n... more Five new species of Listeria (L. floridensis sp. nov, L. aquatica sp. nov., L. cornellensis sp. nov. L. riparia sp. nov., and L. grandensis sp. nov.) from agricultural and natural environments in the United States
International Journal of Systematic and Evolutionary Microbiology, 2014
Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded... more Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average blast nucleotide identity (ANIb) of less than 85 % to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria ; (i) a clade representing Listeria monocytogenes , L. marthii , L. innocua , L. welshimeri , L. seeligeri and L. ivanovii , which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188T = DSM 26686T = LMG 28120T = BEI NR-42633T) and Listeria floridensis sp. nov. (type stra...
Salmonella accounts for approximately 50% of produce-associated outbreaks in the United States, s... more Salmonella accounts for approximately 50% of produce-associated outbreaks in the United States, several of which have been traced back to contamination in the produce production environment. To quantify Salmonella diversity and aid in identification of Salmonella contamination sources, we characterized Salmonella isolates from two geographically diverse produce-growing regions in the United States. Initially, we characterized the Salmonella serotype and subtype diversity associated with 1,677 samples collected from 33 produce farms in New York State (NYS). Among these 1,677 samples, 74 were Salmonella positive, yielding 80 unique isolates (from 147 total isolates), which represented 14 serovars and 23 different pulsed-field gel electrophoresis (PFGE) types. To explore regional Salmonella diversity associated with production environments, we collected a smaller set of samples (n = 65) from South Florida (SFL) production environments and compared the Salmonella diversity associated wi...
ABSTRACTProduce-related outbreaks have been traced back to the preharvest environment. A longitud... more ABSTRACTProduce-related outbreaks have been traced back to the preharvest environment. A longitudinal study was conducted on five farms in New York State to characterize the prevalence, persistence, and diversity of food-borne pathogens in fresh produce fields and to determine landscape and meteorological factors that predict their presence. Produce fields were sampled four times per year for 2 years. A total of 588 samples were analyzed forListeria monocytogenes,Salmonella, and Shiga toxin-producingEscherichia coli(STEC). The prevalence measures ofL. monocytogenes,Salmonella, and STEC were 15.0, 4.6, and 2.7%, respectively.L. monocytogenesandSalmonellawere detected more frequently in water samples, while STEC was detected with equal frequency across all sample types (soil, water, feces, and drag swabs).L. monocytogenes sigBgene allelic types 57, 58, and 61 andSalmonella entericaserovar Cerro were repeatedly isolated from water samples. Soil available water storage (AWS), temperatur...
Five new species of Listeria (L. floridensis sp. nov, L. aquatica sp. nov., L. cornellensis sp. n... more Five new species of Listeria (L. floridensis sp. nov, L. aquatica sp. nov., L. cornellensis sp. nov. L. riparia sp. nov., and L. grandensis sp. nov.) from agricultural and natural environments in the United States
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