The study was conducted to develop an efficient regeneration protocol in tomato through callus in... more The study was conducted to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where the germination rate was 78.4%. The leaves were used as explants. Different concentration and combination of plant growth regulators (PGRs) were added with MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Leaf explants cultured on MS medium fortified with 3 mg/L BAP gave the highest number of shoots (3.5) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (5.149 cm) of plantlets, number (5.5) of leaves and fresh weight (0.781 g) of plantlets with the leaf explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (25.25) of roots/plantlet, length (8.785 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the leaf expl...
analysis of the genotypes based on yield-contributing and yield data revealed different levels of... more analysis of the genotypes based on yield-contributing and yield data revealed different levels of salt tolerance. Notably, BINA-01 and-02, and BINA-04 were recognized as the highest and lowest salt-tolerant genotypes, respectively. Furthermore, exogenous GSH alleviated oxidative stress in the representative contrasting genotypes at the vegetative (V3) stage by decreasing salt-induced accumulation of malondialdehyde and hydrogen peroxide. Taken together, our findings revealed that exogenous GSH application can minimize oxidative stress and contribute to the improvement of yield-contributing parameters, leading to improved yield in soybean genotypes under salt stress. Further investigations on molecular aspects will enable us to gain an indepth understanding of how exogenous GSH can improve salinity tolerance in soybean, particularly at reproductive stage, to discover relevant pathways for biotechnological manipulation.
The experiment was constructed with Sulphur (S) and Boron (B) with three doses of each, i.e., S0:... more The experiment was constructed with Sulphur (S) and Boron (B) with three doses of each, i.e., S0: without Sulphur (control), S20: 20kg S/ha, S30: 30kg S/ha and B0: without Boron (control), B2: 2kg B/ha, B3: 3kg B/ha to study the effect on Boro rice (cv. BRRI dhan-28). In case of Sulphur, the treatment S20 obtained the maximum plant height (97.811cm), maximum tillers hill-1 (26.184) at harvest, highest panicle length (24.98cm), maximum grains panicle-1 (167.37), maximum 1000-grain weight (27.57 g), maximum grain yield (5.46t/ha) and straw yield (5.71t/ha), maximum biological yield (11.17t/ha) and HI (48.88%). Whereas, maximum filled grains panicle-1 (147.37) and minimum unfilled grains panicle-1 (17.89) were found in S30. In case of Boron, the highest plant height (96.378 cm) was found in B3. Highest number of total tiller hill-1 (25.149), longest panicle (24.17 cm), highest number of total grains panicle-1 (165.78), filled grains panicle-1 (144.04) and minimum unfilled grains panicl...
The field study was conducted in three areas of Rajshahi City Corporation during March to August,... more The field study was conducted in three areas of Rajshahi City Corporation during March to August, 2014 to investigate the vegetative growth potentials of parthenium on the basis of different plant species and land. Parthenium hysterophorus L. is a weed of Asteraceae family possesses both scopes and hazards. This weed is mostly known for its harmful roles as environmental, medical and agricultural hazards. Parthenium contributes many health benefits, bioremediation, aquatic weeds eradication, use as biofuel and biopesticide. In this study high coverage of parthenium was found at the perennial barren and waste land. At Talaimari region both Cassia tora and Euphorbia hirta were highest (96%) in abundance where Parthenium did not invade but 82% and 96% respectively at the invaded region. In case of the area of University of Rajshahi Imperata cylindrical (100%) at parthenium non invaded region and Euphorbia hirta (94%) after parthenium (100%). At Shyampur area Trifolia repens was highest (100%) in abundance at both parthenium invaded and noninvaded area and Euphorbia hirta, Imperata cylindrical and parthenium were abundant at100%. The study facilitates the beneficial use and hazard management as well as taking effective control measures of parthenium in Rajshahi City Corporation.
Abstract: A pot experiment was carried out at the grill house of the Department of Crop Botany, B... more Abstract: A pot experiment was carried out at the grill house of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh during the period from December 2013 to April 2014 to study the growth, morpho-physiological, yield and yield contributing characters of twenty sorghum genotypes. The experiment was consisted of twenty genotypes of sorghum viz., BD 700, BD 701, BD 702, BD703, BD704, BD705, BD706, BD707, BD708, BD709, BD710, BD712, BD720, BD721, BD722, BD725, BD726, BD727, BD728 and BD729. The experiment was laid out in a Completely Randomized Design with three replications. The collected data were analyzed statistically and the means were adjudged by Duncan's multiple range test at 1% level of probability. Among the genotypes, BD 725 showed significantly the best performance on growth, yield and morphophysiological characters compare to other genotypes at harvest, while BD728 was less efficient among those parameters. As a result, highest plant height (2...
Asian Journal of Medical and Biological Research, 2016
The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Labora... more The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali. The objective was to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where germination rate was 78.4%. The stems of in vitrocultured seedlings were used as explants. Different concentrations and combinations of growth regulators were added to MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Stem explants cultured on MS medium fortified with 2 mg/L BAPgave the highest number of shoots (3.0) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (4.064 cm) of plantlets, number (5.0) of leaves and fresh weight (0.663 g) of plantlets with the stem explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest num...
Abstract: An experiment was conducted in randomized complete block design (RCBD) with three repli... more Abstract: An experiment was conducted in randomized complete block design (RCBD) with three replications in the experimental field of Regional Horticulture Research Station (RHRS), Bangladesh Agriculture Research Institute (BARI), Lebukhali, Patuakhali during April, 2014 to October, 2014 for assessing the inbreeding depression, genetic parameters, gene action and segregation pattern of Okra [Abelmoschusesculentus (L.) Moench]. The experiment was comprised of five commercial hybrid Okra genotypes such as Tara sonali, Bimala, Juboraj, Suvo 1and Noor, their respective F 2 progenies along with a check variety named as BARI Dherosh 1. Results of the experiment indicated that there were considerable variability among the F 1 and their F 2 . The yield were in-between 14.81 to 7.92 Kg plot -1 in case of F 1 generation, which deteriorate to 10.32 to 5.32 Kg plot -1 in F 2 generation. Broad sense heritability computed through variance component method showed that all the quantitative traits w...
analysis of the genotypes based on yield-contributing and yield data revealed different levels of... more analysis of the genotypes based on yield-contributing and yield data revealed different levels of salt tolerance. Notably , BINA-01 and-02, and BINA-04 were recognized as the highest and lowest salt-tolerant genotypes, respectively. Furthermore, exogenous GSH alleviated oxidative stress in the representative contrasting genotypes at the vegeta-tive (V3) stage by decreasing salt-induced accumulation of malondialdehyde and hydrogen peroxide. Taken together, our findings revealed that exogenous GSH application can minimize oxidative stress and contribute to the improvement of yield-contributing parameters, leading to improved yield in soybean genotypes under salt stress. Further investigations on molecular aspects will enable us to gain an in-depth understanding of how exogenous GSH can improve salinity tolerance in soybean, particularly at reproductive stage, to discover relevant pathways for biotechnological manipulation.
The study was conducted to develop an efficient regeneration protocol in tomato through callus in... more The study was conducted to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where the germination rate was 78.4%. The leaves were used as explants. Different concentration and combination of plant growth regulators (PGRs) were added with MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Leaf explants cultured on MS medium fortified with 3 mg/L BAP gave the highest number of shoots (3.5) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (5.149 cm) of plantlets, number (5.5) of leaves and fresh weight (0.781 g) of plantlets with the leaf explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (25.25) of roots/plantlet, length (8.785 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the leaf expl...
analysis of the genotypes based on yield-contributing and yield data revealed different levels of... more analysis of the genotypes based on yield-contributing and yield data revealed different levels of salt tolerance. Notably, BINA-01 and-02, and BINA-04 were recognized as the highest and lowest salt-tolerant genotypes, respectively. Furthermore, exogenous GSH alleviated oxidative stress in the representative contrasting genotypes at the vegetative (V3) stage by decreasing salt-induced accumulation of malondialdehyde and hydrogen peroxide. Taken together, our findings revealed that exogenous GSH application can minimize oxidative stress and contribute to the improvement of yield-contributing parameters, leading to improved yield in soybean genotypes under salt stress. Further investigations on molecular aspects will enable us to gain an indepth understanding of how exogenous GSH can improve salinity tolerance in soybean, particularly at reproductive stage, to discover relevant pathways for biotechnological manipulation.
The experiment was constructed with Sulphur (S) and Boron (B) with three doses of each, i.e., S0:... more The experiment was constructed with Sulphur (S) and Boron (B) with three doses of each, i.e., S0: without Sulphur (control), S20: 20kg S/ha, S30: 30kg S/ha and B0: without Boron (control), B2: 2kg B/ha, B3: 3kg B/ha to study the effect on Boro rice (cv. BRRI dhan-28). In case of Sulphur, the treatment S20 obtained the maximum plant height (97.811cm), maximum tillers hill-1 (26.184) at harvest, highest panicle length (24.98cm), maximum grains panicle-1 (167.37), maximum 1000-grain weight (27.57 g), maximum grain yield (5.46t/ha) and straw yield (5.71t/ha), maximum biological yield (11.17t/ha) and HI (48.88%). Whereas, maximum filled grains panicle-1 (147.37) and minimum unfilled grains panicle-1 (17.89) were found in S30. In case of Boron, the highest plant height (96.378 cm) was found in B3. Highest number of total tiller hill-1 (25.149), longest panicle (24.17 cm), highest number of total grains panicle-1 (165.78), filled grains panicle-1 (144.04) and minimum unfilled grains panicl...
The field study was conducted in three areas of Rajshahi City Corporation during March to August,... more The field study was conducted in three areas of Rajshahi City Corporation during March to August, 2014 to investigate the vegetative growth potentials of parthenium on the basis of different plant species and land. Parthenium hysterophorus L. is a weed of Asteraceae family possesses both scopes and hazards. This weed is mostly known for its harmful roles as environmental, medical and agricultural hazards. Parthenium contributes many health benefits, bioremediation, aquatic weeds eradication, use as biofuel and biopesticide. In this study high coverage of parthenium was found at the perennial barren and waste land. At Talaimari region both Cassia tora and Euphorbia hirta were highest (96%) in abundance where Parthenium did not invade but 82% and 96% respectively at the invaded region. In case of the area of University of Rajshahi Imperata cylindrical (100%) at parthenium non invaded region and Euphorbia hirta (94%) after parthenium (100%). At Shyampur area Trifolia repens was highest (100%) in abundance at both parthenium invaded and noninvaded area and Euphorbia hirta, Imperata cylindrical and parthenium were abundant at100%. The study facilitates the beneficial use and hazard management as well as taking effective control measures of parthenium in Rajshahi City Corporation.
Abstract: A pot experiment was carried out at the grill house of the Department of Crop Botany, B... more Abstract: A pot experiment was carried out at the grill house of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh during the period from December 2013 to April 2014 to study the growth, morpho-physiological, yield and yield contributing characters of twenty sorghum genotypes. The experiment was consisted of twenty genotypes of sorghum viz., BD 700, BD 701, BD 702, BD703, BD704, BD705, BD706, BD707, BD708, BD709, BD710, BD712, BD720, BD721, BD722, BD725, BD726, BD727, BD728 and BD729. The experiment was laid out in a Completely Randomized Design with three replications. The collected data were analyzed statistically and the means were adjudged by Duncan's multiple range test at 1% level of probability. Among the genotypes, BD 725 showed significantly the best performance on growth, yield and morphophysiological characters compare to other genotypes at harvest, while BD728 was less efficient among those parameters. As a result, highest plant height (2...
Asian Journal of Medical and Biological Research, 2016
The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Labora... more The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali. The objective was to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where germination rate was 78.4%. The stems of in vitrocultured seedlings were used as explants. Different concentrations and combinations of growth regulators were added to MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Stem explants cultured on MS medium fortified with 2 mg/L BAPgave the highest number of shoots (3.0) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (4.064 cm) of plantlets, number (5.0) of leaves and fresh weight (0.663 g) of plantlets with the stem explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest num...
Abstract: An experiment was conducted in randomized complete block design (RCBD) with three repli... more Abstract: An experiment was conducted in randomized complete block design (RCBD) with three replications in the experimental field of Regional Horticulture Research Station (RHRS), Bangladesh Agriculture Research Institute (BARI), Lebukhali, Patuakhali during April, 2014 to October, 2014 for assessing the inbreeding depression, genetic parameters, gene action and segregation pattern of Okra [Abelmoschusesculentus (L.) Moench]. The experiment was comprised of five commercial hybrid Okra genotypes such as Tara sonali, Bimala, Juboraj, Suvo 1and Noor, their respective F 2 progenies along with a check variety named as BARI Dherosh 1. Results of the experiment indicated that there were considerable variability among the F 1 and their F 2 . The yield were in-between 14.81 to 7.92 Kg plot -1 in case of F 1 generation, which deteriorate to 10.32 to 5.32 Kg plot -1 in F 2 generation. Broad sense heritability computed through variance component method showed that all the quantitative traits w...
analysis of the genotypes based on yield-contributing and yield data revealed different levels of... more analysis of the genotypes based on yield-contributing and yield data revealed different levels of salt tolerance. Notably , BINA-01 and-02, and BINA-04 were recognized as the highest and lowest salt-tolerant genotypes, respectively. Furthermore, exogenous GSH alleviated oxidative stress in the representative contrasting genotypes at the vegeta-tive (V3) stage by decreasing salt-induced accumulation of malondialdehyde and hydrogen peroxide. Taken together, our findings revealed that exogenous GSH application can minimize oxidative stress and contribute to the improvement of yield-contributing parameters, leading to improved yield in soybean genotypes under salt stress. Further investigations on molecular aspects will enable us to gain an in-depth understanding of how exogenous GSH can improve salinity tolerance in soybean, particularly at reproductive stage, to discover relevant pathways for biotechnological manipulation.
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