This study aimed to evaluate the impact of Basella rubra fruit extract (BR-FE) on cryopreserved r... more This study aimed to evaluate the impact of Basella rubra fruit extract (BR-FE) on cryopreserved ram sperm's motility, velocity, and membrane integrity. Thirty ejaculates collected from 3 fertile rams (10 from each) were diluted with semen dilution extender (SDE) in a ratio (1:2) and centrifuged to remove 50% supernatant. The remaining sample was mixed with semen cryopreservation extender (SCE) in 1:4 ratio. Then 1.2 mL of SCE diluted sample was divided in four aliquots (0.3 mL each) that were further extended with [(1) control group (0.7 mL of SCE), (2) BR-FE-0.6% group (0.7 mL of SCE supplemented with 0.6% BR-FE), (3) BR-FE-0.8% group (0.7 mL of SCE supplemented with 0.8% BR-FE), and (4) BR-FE-1.6% group (0.7 mL SCE supplemented with 1.6% BR-FE)]. All extended samples were cooled gradually from 25°C to 4°C in half an hour. The 0.1 mL sample from all aliquots was analyzed for precryopreservation sperm parameters and the remaining sample was loaded in 0.5 mL plastic semen straws, cooled gradually to-20°C, and then dipped in liquid nitrogen. After 24 hours of cryopreservation, the straws were thawed for postcryopreservation sperm evaluations. The results (analysis of variance based) showed significantly enhanced percentage of post-thaw sperm membrane integrity, progressive motility, and velocity in BR-FE-0.6% group at both pre-and postcryopreservation stages as compared with all other groups. However, analysis of covariance revealed concentration-dependent cryoprotective effect of BR-FE with maximum percentage of sperm membrane integrity in the 1.6% group. According to these results, BR-FE supplementation adds enormous sperm protective potential to ram sperm cryopreservation medium.
The ameliorative prospective of Jambul (Syzygium cumini) fruit pulp extract was studied against P... more The ameliorative prospective of Jambul (Syzygium cumini) fruit pulp extract was studied against Pb-based hepato-histological and micrometric changes in albino laboratory mice (Mus musculus). There were 3 experimental groups: (i) Control group (untreated) (ii) Pb group (15 days 50ppm Pb ions from Lead acetate in drinking water (iii) Pb+Jm group (Pb ions treatment as in Pb group followed by 0.2ml/per day jambul fruit pulp extract through gavage and plain water for the next 5 days). Animals were euthanized on the 21st day to recover liver for histopathological and micrometric studies. The misalignment of the hepatic cord with narrowed and chocked sinusoidal spaces and some lesions in hepatic tissue were seen as persistent histopathological signs of lead exposure. Micrometric observations indicate that the mean cross-sectional area of the hepatocytes in the Pb group (483.5µ2) increased significantly than the control group (420.1µ2), similarly, the mean cross-sectional area of the hepato...
This study aimed to evaluate the impact of Basella rubra fruit extract (BR-FE) on cryopreserved r... more This study aimed to evaluate the impact of Basella rubra fruit extract (BR-FE) on cryopreserved ram sperm's motility, velocity, and membrane integrity. Thirty ejaculates collected from 3 fertile rams (10 from each) were diluted with semen dilution extender (SDE) in a ratio (1:2) and centrifuged to remove 50% supernatant. The remaining sample was mixed with semen cryopreservation extender (SCE) in 1:4 ratio. Then 1.2 mL of SCE diluted sample was divided in four aliquots (0.3 mL each) that were further extended with [(1) control group (0.7 mL of SCE), (2) BR-FE-0.6% group (0.7 mL of SCE supplemented with 0.6% BR-FE), (3) BR-FE-0.8% group (0.7 mL of SCE supplemented with 0.8% BR-FE), and (4) BR-FE-1.6% group (0.7 mL SCE supplemented with 1.6% BR-FE)]. All extended samples were cooled gradually from 25°C to 4°C in half an hour. The 0.1 mL sample from all aliquots was analyzed for precryopreservation sperm parameters and the remaining sample was loaded in 0.5 mL plastic semen straws, cooled gradually to-20°C, and then dipped in liquid nitrogen. After 24 hours of cryopreservation, the straws were thawed for postcryopreservation sperm evaluations. The results (analysis of variance based) showed significantly enhanced percentage of post-thaw sperm membrane integrity, progressive motility, and velocity in BR-FE-0.6% group at both pre-and postcryopreservation stages as compared with all other groups. However, analysis of covariance revealed concentration-dependent cryoprotective effect of BR-FE with maximum percentage of sperm membrane integrity in the 1.6% group. According to these results, BR-FE supplementation adds enormous sperm protective potential to ram sperm cryopreservation medium.
The ameliorative prospective of Jambul (Syzygium cumini) fruit pulp extract was studied against P... more The ameliorative prospective of Jambul (Syzygium cumini) fruit pulp extract was studied against Pb-based hepato-histological and micrometric changes in albino laboratory mice (Mus musculus). There were 3 experimental groups: (i) Control group (untreated) (ii) Pb group (15 days 50ppm Pb ions from Lead acetate in drinking water (iii) Pb+Jm group (Pb ions treatment as in Pb group followed by 0.2ml/per day jambul fruit pulp extract through gavage and plain water for the next 5 days). Animals were euthanized on the 21st day to recover liver for histopathological and micrometric studies. The misalignment of the hepatic cord with narrowed and chocked sinusoidal spaces and some lesions in hepatic tissue were seen as persistent histopathological signs of lead exposure. Micrometric observations indicate that the mean cross-sectional area of the hepatocytes in the Pb group (483.5µ2) increased significantly than the control group (420.1µ2), similarly, the mean cross-sectional area of the hepato...
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Papers by Saima Matloob