... 10. Mert A, Tabak F, Dumankar A, Eroğlu C, Öztürk R, Aktuğlu Y, 1997. Dört Marsilya humması o... more ... 10. Mert A, Tabak F, Dumankar A, Eroğlu C, Öztürk R, Aktuğlu Y, 1997. Dört Marsilya humması olgusu. Klimik Derg, 10:146-8. 11. Mert A, Özaras R, Tabak F, Bilir M, Öztürk R, Aktuğlu Y, 2003. Akdeniz benekli ateşi: 14 olgunun değerlendirmesi. Flora, 8:158-62. 12. ...
Corneal transplantation is an important treatment method for various corneal disorders. 1 It is t... more Corneal transplantation is an important treatment method for various corneal disorders. 1 It is the most widely used clinical transplantation method. 2 Fungal infections of the Candida, Fusarium, and Aspergillus spp. are the frequently isolated organisms in fungal keratitis. 3 Candida species, especially Candida albicans, are responsible for the majority of reported cases of post-keratoplasty fungal keratitis. 4 Cladosporium spp. usually cause allergic rhinitis or supericial lesions. 5 It is also an opportunistic pathogen in humans. 6
Journal of Antimicrobial Chemotherapy, Apr 21, 2022
Objectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fum... more Objectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.
Flora the Journal of Infectious Diseases and Clinical Microbiology
çıkan ve tüm dünyaya yayılan COVID-19 pandemisi ile birlikte birçok merkez tarafından hızla aşı ç... more çıkan ve tüm dünyaya yayılan COVID-19 pandemisi ile birlikte birçok merkez tarafından hızla aşı çalışmalarına başlanmıştır. Bunlardan biri de Sinovac firmasının inaktif CoronaVac aşısıdır. Türkiye CoronaVac aşısına acil kullanım onayı vermiştir. Bu çalışmada üniversitemizde COVID-19 açısından yüksek riskli bölümlerde görev yapan ve CoronaVac ile aşılanan sağlık çalışanlarında aşıya antikor yanıtlarının araştırılması hedeflenmiştir. Çalışmaya katılan sağlık personeli aşı grubu ve kontrol grubu olarak ayrılmıştır. Çalışmamıza aşı grubundan 41 kişi, kontrol grubundan 12 kişi gönüllü olmuştur. Birinci doz aşıdan 14 gün sonra alınan aşı ve kontrol grubu numuneleri anti-SARS-CoV-2 IgG (Euroimmun, Almanya) ile çalışılmıştır. Aşı grubundaki dört kişinin istifa etme ve izinli olma gibi nedenlerle ikinci doz aşı sonrası antikor seviyelerine bakılamamıştır. İkinci doz aşı sonrası kontrol grubunda antikor seviyesi bakılmamıştır. Birinci doz aşıdan sonraki antikor seviyesi aşı grubunda ve kontrol grubunda sırasıyla %9.8 ve %0 olarak bulunmuştur. İkinci doz aşıdan sonra ise aşı grubunda bu oran %94.6 olarak saptanmıştır. Mevcut bulgularla tek doz aşının çalışma grubunda istenilen oranda antikor yanıtı oluşturamadığı görülmüştür. Aşı programı tamamlansa ve koruyucu antikor oluşsa da toplumsal bağışıklık istenen oranlara gelene kadar kişisel korunma kurallarına uymak COVID-19 hastalığından korunmada elzemdir.
Introduction Francisella tularensis is a Gram-negative aerobic bacillus and is the causative agen... more Introduction Francisella tularensis is a Gram-negative aerobic bacillus and is the causative agent of tularemia. It was discovered by Edward Francis and named after Tulare County, where it was discovered. Tularemia, also known as rabbit fever or hunter's disease, is a disease mostly seen in rural areas. Transmission occurs through gastrointestinal system, respiratory system, or direct skin contact with rabbits, rodents, and blood-sucking arthropods. Infection can occur in typhoidal, pneumonic, oculoglandular, oropharyngeal, ulceroglandular, and glandular forms. Although the incubation period varies between 1 and 21 days, it usually lasts 3-5 days. Symptoms vary according to the form of the infection [1]. According to Egyptian records, tularemia emerged in the Canaan Region (Lebanon, Syria, Jordan, and Israel), Background/aim: According to Egyptian records, tularemia emerged in the Canaan region, where it was first identified and spread to Anatolia over the Euphrates. It was used as an active biological weapon for the first time in the Hittite-Arzawa War in 1320-1318 BC. This study aimed to investigate the seroprevalence of tularemia in the Inner Aegean Region, which is thought to be the region where this war was fought 3300 years ago. Materials and methods: Tularemia seropositivity in humans was investigated in 27 villages/neighborhoods in 3 districts in each of Manisa, Kütahya, and Uşak provinces. Before the study, the participants were informed about the disease via posters, and their blood samples were taken following filling out the questionnaire. Microagglutination tests were performed using in-house tularemia antigen and V plate for serological experiments. Rose-Bengal test was also performed on seropositive sera. Results: Of the total of 410 people, 226 (55.12%) were male. The mean age of the volunteers was 43.72 years. The highest participation was from Kütahya Province. According to the results of the tularemia microagglutination test, seropositivity was detected in 6 cases. It was determined that all of the seropositive volunteers were in Kütahya. When the tularemia antibody titers were examined, seropositivity was determined at 1/20-1/160 titers. No positivity was detected in the Rose-Bengal test for cross-reaction. Conclusion: Kütahya has been identified as a risky region in terms of tularemia in the Inner Aegean Region. In order to use the resources in the country economically, first of all, the risk areas in terms of tularemia should be determined by serological studies in all regions. In order to increase awareness about the disease, physicians and filiation teams should be trained in risky areas. Surveillance studies should be conducted to identify and monitor possible sources in areas identified as risky.
To investigate the features of a new tularemia outbreak that occurred in the Th race region. Mate... more To investigate the features of a new tularemia outbreak that occurred in the Th race region. Materials and methods: Th e research team visited the village aft er the identifi cation of the index case. Serum and throat samples were taken from 41 villagers who were examined, and environmental samples were taken in order to identify the source of the outbreak. Culture, serology, and molecular methods were used to search for Francisella tularensis in these samples. Results: A total of 8 children were diagnosed with tularemia. Th e adults and all of the other children were seronegative for tularemia. All of the patients had a history of swimming in a pool fi lled with water from a local stream, and contact with stream water was calculated to increase the risk of developing the disease 9.3-fold. Polymerase chain reaction analysis was positive in a lymph node aspirate of the index case and in the home tap water of 3 patients as well as in the spring water and stream water in the village. Francisella tularensis could not be isolated from any culture of samples. Interestingly, the waterborne tularemia outbreak aff ected only children. Conclusion: Although tularemia has been not reported from Tekirdağ Province for 74 years, the disease reemerged in the region due to the removal of hygienic measures. Th ese clues may signify that the agent had maintained its presence in the region for many years.
Koronavirüs hastalığı-2019 (COVID-19) salgını ilk olarak Çin'de başlamış olup tüm dünyaya yayılmı... more Koronavirüs hastalığı-2019 (COVID-19) salgını ilk olarak Çin'de başlamış olup tüm dünyaya yayılmıştır. Ülkemizde salgın yönetimi Sağlık Bakanlığı koordinesinde yapılmaktadır. İlimizdeki olgu artış hızının aylar içinde değişmesiyle birlikte hastanemizde oluşan ihtiyaçlara tıbbi mikrobiyoloji laboratuvarı olarak eksiksiz cevap verilmiştir. Bu çalışmada bu deneyimlerimizi aktarmak ve pandeminin ilk yedi ayında koronavirüs laboratuvarına gelen örneklerin retrospektif analizini yapmak amaçlanmıştır. Gereç ve Yöntem: Pandemi sürecinde güvenli bir çalışma ortamı oluşturmak ve iş akışını organize etmek için laboratuvar içinde birtakım değişiklikler yapılıp rehber haline getirilmiştir. Ayrıca 21.03.2020-21.10.2020 tarihleri arasında koronavirüs laboratuvarına gelen Şiddetli akut solunum sendromu koronavirüs-2 (SARS-CoV-2) polimeraz zincir reaksiyonu (PZR) ve anti-SARS-CoV-2 antikor testleri retrospektif olarak taranmıştır. Bulgular: Laboratuvarımızda toplam 73.773 SARS-CoV-2 PZR testi, 1.170 anti SARS-CoV-2 antikor testi çalışılmıştır. Toplam PZR pozitifliğinin %2,7, antikor pozitifliğinin ise %1,9 olduğu tespit edilmiştir. Sonuç: PZR pozitiflik oranları incelendiğinde alınan önlemler ile ilk aydan sonra dramatik bir düşük gözlenmekte ve bu düşüş beşinci aya kadar devam etmektedir. Önlemlerin gevşetilmesi ile altıncı ve yedinci aylarda PZR pozitifliğinin tekrar yükseldiği görülmektedir. Pandeminin ilk aylarında elde ettiğimiz deneyimlerimizin diğer laboratuvarlar için değerli bilgiler sağlayabileceği düşünülmektedir.
Amac: Bu calismada Edirne Merkez Ilkogretim Okullari ogrencilerinde pedikulus humanus kapitis ve ... more Amac: Bu calismada Edirne Merkez Ilkogretim Okullari ogrencilerinde pedikulus humanus kapitis ve tinea kapitisin gorulme sikligi ve tinea kapitis yapan etkenlerin saptanmasi amaclandi. Olgular ve Yontemler: 2003 yili Nisan ve Mayis aylarinda Edirne merkezindeki 34 ilkogretim okulunda 12.868 ogrenci tarandi. Sac ve sacli deride lezyon gorulenlerin lezyonlu bolgelerinden, sac teli ve sacli derilerinden kazinti ornegi alindi. Alinan orneklerden KOH ile direkt mikroskobik inceleme ve sikloheksimidli Sabouraud dekstroz agarda mantar kulturu yapildi. Bulgular: Toplam 698 (%5.4) ogrencinin basinda pedikulusun sirke, nimf veya olgun sekli saptandi. Kiz ogrencilerdeki pozitiflik (%10.3) erkek ogrencilerdekinden (%0.9) daha yuksekti (p < 0.001). Enfestasyon orani en az yedi yas altinda (%3.4) bulundu (p < 0.001). Sosyo-ekonomik duzeyi dusuk ailelerin bulundugu bolgelerde bitlenme orani daha yuksekti (p < 0.001). Hicbir ogrencide tinea kapitise rastlanmadi. Sonuc: Pedikulus humanus ka...
Lysinibacillus massiliensis is an aerobic, endospore-forming, gram-negative staining bacterium wi... more Lysinibacillus massiliensis is an aerobic, endospore-forming, gram-negative staining bacterium with peritrichous flagella belonging to the Bacillaceae family. A few cases of L. massiliensis isolated from the cerebrospinal fluid and tissue have been reported. In this study, we aimed to describe a case of L. massiliensis isolated from the synovial fluid. The synovial fluid from a 74-year-old female patient was inoculated into blood culture bottle. Gram-negative rods were observed in a gram-stained smear from a positive blood culture bottle. The bacterium was identified as Lysinibacillus sphaericus/Lysinibacillus fusiformis, with a probability of 89% using an automated bacterial identification system (VITEK2; Biomerieux, France). Subsequently, 16S rRNA gene sequencing was performed, and the sequence was analyzed using the Basic Local Alignment Search Tool. The sequence had 99.9% (1426/1427) identity with the strain L. massiliensis (GenBank ID: NR_043092.1). To our knowledge, this is th...
Candida tropicalis is the fourth leading cause of candidemia in Turkey. Although C. tropicalis is... more Candida tropicalis is the fourth leading cause of candidemia in Turkey. Although C. tropicalis isolates from 1997 to 2017 were characterized as fully susceptible to antifungals, the increasing global prevalence of azole-non-susceptible (ANS) C. tropicalis and the association between high fluconazole tolerance (HFT) and fluconazole therapeutic failure (FTF) prompted us to re-evaluate azole susceptibility of C. tropicalis in Turkey. In this study, 161 C. tropicalis blood isolates from seven clinical centers were identified by ITS rDNA sequencing, genotyped by multilocus microsatellite typing, and tested for susceptibility to five azoles, two echinocandins, and amphotericin B (AMB); antifungal resistance mechanisms were assessed by sequencing of ERG11 and FKS1 genes. The results indicated that C. tropicalis isolates, which belonged to 125 genotypes grouped into 11 clusters, were fully susceptible to echinocandins and AMB; however, 18.6% of them had the ANS phenotype but only two carried the ANS-conferring mutation (Y132F). HFT was recorded in 52 isolates, 10 of which were also ANS. Large proportions of patients infected with ANS and HFT isolates (89 and 40.7%, respectively) showed FTF. Patients infected with azole-susceptible or ANS isolates did not differ in
Colistin is a polymyxin antibiotic which is considered as one of the last line agents against inf... more Colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmid-mediated colistin resistance gene mcr-2 in 2016. The rapid and interspecies dissemination of plasmid-mediated resistance mechanisms through horizontal gene transfer, have made these genes considerably threatening. After the first reports, although mcr-1/mcr-2 producing Enterobacteriaceae isolates have been reported from many countries, there have been no reports from Turkey. Thus, the aim of this study was to investigate the presence of mcr-1/mcr-2 in clinical Enterobacteriaceae ...
Objectives: It was aimed to investigate anaplasmosis seropositivity in people exposured to tick b... more Objectives: It was aimed to investigate anaplasmosis seropositivity in people exposured to tick bite. Materials and Methods: A total of 116 individuals (89 males, 27 females; mean age 43; range 6 to 88 years) with tick bite history in rural areas of Thrace Region were included in this study. Possible risk factors were evaluated by a questionnaire. Sera obtained from volunteers were preserved in andash;70˚C deep freezer until the study is done. Antibodies against Anaplasma phagocytophilum were investigated by indirect fluorescent antibody (IFA) test in the sera. Results: Antibodies against A. phagocytophilum were positive for 29 individuals (25%). Contact with horse/donkey is defined as a risk factor for anaplasmosis seropositivity. Conclusion: Antibodies against A. phagocytophilum were found at high rates in people exposured to tick bite in rural areas of Thrace Region. Amac: Kene isirigi oykusu olan kisilerde anaplazmoz seropozitifliginin arastirilmasi amaclandi. Gerecler ve Yontemler: Trakya Bolgesi kirsal alanlarinda kene isirigi oykusu olan 116 kisi (89 erkek, 27 kadin; ort. yas 43; dagilim 6-88) calismaya alindi. Bir anket formu ile risk faktoru olabilecek durumlar sorgulandi. Gonullulerden alinan serumlar calisma yapilincaya kadar andash;70˚Ca#39;lik derin dondurucuda saklandi. Serumlarda indirekt floresan antikor (IFA) yontemi ile Anaplasma phagocytophilum antikorlari arastirildi. Bulgular: A. phagocytophilum antikorlari 29 kiside (%25) pozitif bulundu. Anaplazmoz seropozitifligi icin at/esek temasi bir risk faktoru olarak belirlendi. Sonuc: Trakya Bolgesia#39;nin kirsal alanlarinda yasayan, kene ile isirilmis insanlarda A. phagocytophiluma#39;a karsi gelisen antikorlar yuksek oranlarda saptanmistir.
Tularemia is caused by Francisella tularensis which is a zoonosis frequently fatal in animals suc... more Tularemia is caused by Francisella tularensis which is a zoonosis frequently fatal in animals such as rats, guinea pigs, rabbits etc., but rarely fatal in humans (1). This disease may occur as an epidemic or can be seen sporadically. The biggest epidemic defined in Turkey started in the vicinity of Bursa in 1988, then other cases were reported from the Black Sea Region, Ankara, and more recently from Kocaeli and Konya (2). The first outbreak of tularemia occurred in the European part of Turkey (3, 4). This outbreak area included 34 villages of Kırklareli and Tekirda¤ Provinces in 1936 as well as Lüleburgaz, a district of Kırklareli. A second outbreak reemerged also in Lüleburgaz in 1945. A total of 150 and 18 patients were reported in the first and second outbreaks, respectively (3-5). After that time, tularemia has not been reported in this region. We present here the first case of tularemia diagnosed in 60 years.
Induced molting is a management tool to prepare laying hens for a second reproduction period. In ... more Induced molting is a management tool to prepare laying hens for a second reproduction period. In commercial layer flocks, aged and unprofitable hens are either molted to obtain heavier, high-quality, marketable eggs [1] or production is continued by pullet replacement,
... 10. Mert A, Tabak F, Dumankar A, Eroğlu C, Öztürk R, Aktuğlu Y, 1997. Dört Marsilya humması o... more ... 10. Mert A, Tabak F, Dumankar A, Eroğlu C, Öztürk R, Aktuğlu Y, 1997. Dört Marsilya humması olgusu. Klimik Derg, 10:146-8. 11. Mert A, Özaras R, Tabak F, Bilir M, Öztürk R, Aktuğlu Y, 2003. Akdeniz benekli ateşi: 14 olgunun değerlendirmesi. Flora, 8:158-62. 12. ...
Corneal transplantation is an important treatment method for various corneal disorders. 1 It is t... more Corneal transplantation is an important treatment method for various corneal disorders. 1 It is the most widely used clinical transplantation method. 2 Fungal infections of the Candida, Fusarium, and Aspergillus spp. are the frequently isolated organisms in fungal keratitis. 3 Candida species, especially Candida albicans, are responsible for the majority of reported cases of post-keratoplasty fungal keratitis. 4 Cladosporium spp. usually cause allergic rhinitis or supericial lesions. 5 It is also an opportunistic pathogen in humans. 6
Journal of Antimicrobial Chemotherapy, Apr 21, 2022
Objectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fum... more Objectives Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.
Flora the Journal of Infectious Diseases and Clinical Microbiology
çıkan ve tüm dünyaya yayılan COVID-19 pandemisi ile birlikte birçok merkez tarafından hızla aşı ç... more çıkan ve tüm dünyaya yayılan COVID-19 pandemisi ile birlikte birçok merkez tarafından hızla aşı çalışmalarına başlanmıştır. Bunlardan biri de Sinovac firmasının inaktif CoronaVac aşısıdır. Türkiye CoronaVac aşısına acil kullanım onayı vermiştir. Bu çalışmada üniversitemizde COVID-19 açısından yüksek riskli bölümlerde görev yapan ve CoronaVac ile aşılanan sağlık çalışanlarında aşıya antikor yanıtlarının araştırılması hedeflenmiştir. Çalışmaya katılan sağlık personeli aşı grubu ve kontrol grubu olarak ayrılmıştır. Çalışmamıza aşı grubundan 41 kişi, kontrol grubundan 12 kişi gönüllü olmuştur. Birinci doz aşıdan 14 gün sonra alınan aşı ve kontrol grubu numuneleri anti-SARS-CoV-2 IgG (Euroimmun, Almanya) ile çalışılmıştır. Aşı grubundaki dört kişinin istifa etme ve izinli olma gibi nedenlerle ikinci doz aşı sonrası antikor seviyelerine bakılamamıştır. İkinci doz aşı sonrası kontrol grubunda antikor seviyesi bakılmamıştır. Birinci doz aşıdan sonraki antikor seviyesi aşı grubunda ve kontrol grubunda sırasıyla %9.8 ve %0 olarak bulunmuştur. İkinci doz aşıdan sonra ise aşı grubunda bu oran %94.6 olarak saptanmıştır. Mevcut bulgularla tek doz aşının çalışma grubunda istenilen oranda antikor yanıtı oluşturamadığı görülmüştür. Aşı programı tamamlansa ve koruyucu antikor oluşsa da toplumsal bağışıklık istenen oranlara gelene kadar kişisel korunma kurallarına uymak COVID-19 hastalığından korunmada elzemdir.
Introduction Francisella tularensis is a Gram-negative aerobic bacillus and is the causative agen... more Introduction Francisella tularensis is a Gram-negative aerobic bacillus and is the causative agent of tularemia. It was discovered by Edward Francis and named after Tulare County, where it was discovered. Tularemia, also known as rabbit fever or hunter's disease, is a disease mostly seen in rural areas. Transmission occurs through gastrointestinal system, respiratory system, or direct skin contact with rabbits, rodents, and blood-sucking arthropods. Infection can occur in typhoidal, pneumonic, oculoglandular, oropharyngeal, ulceroglandular, and glandular forms. Although the incubation period varies between 1 and 21 days, it usually lasts 3-5 days. Symptoms vary according to the form of the infection [1]. According to Egyptian records, tularemia emerged in the Canaan Region (Lebanon, Syria, Jordan, and Israel), Background/aim: According to Egyptian records, tularemia emerged in the Canaan region, where it was first identified and spread to Anatolia over the Euphrates. It was used as an active biological weapon for the first time in the Hittite-Arzawa War in 1320-1318 BC. This study aimed to investigate the seroprevalence of tularemia in the Inner Aegean Region, which is thought to be the region where this war was fought 3300 years ago. Materials and methods: Tularemia seropositivity in humans was investigated in 27 villages/neighborhoods in 3 districts in each of Manisa, Kütahya, and Uşak provinces. Before the study, the participants were informed about the disease via posters, and their blood samples were taken following filling out the questionnaire. Microagglutination tests were performed using in-house tularemia antigen and V plate for serological experiments. Rose-Bengal test was also performed on seropositive sera. Results: Of the total of 410 people, 226 (55.12%) were male. The mean age of the volunteers was 43.72 years. The highest participation was from Kütahya Province. According to the results of the tularemia microagglutination test, seropositivity was detected in 6 cases. It was determined that all of the seropositive volunteers were in Kütahya. When the tularemia antibody titers were examined, seropositivity was determined at 1/20-1/160 titers. No positivity was detected in the Rose-Bengal test for cross-reaction. Conclusion: Kütahya has been identified as a risky region in terms of tularemia in the Inner Aegean Region. In order to use the resources in the country economically, first of all, the risk areas in terms of tularemia should be determined by serological studies in all regions. In order to increase awareness about the disease, physicians and filiation teams should be trained in risky areas. Surveillance studies should be conducted to identify and monitor possible sources in areas identified as risky.
To investigate the features of a new tularemia outbreak that occurred in the Th race region. Mate... more To investigate the features of a new tularemia outbreak that occurred in the Th race region. Materials and methods: Th e research team visited the village aft er the identifi cation of the index case. Serum and throat samples were taken from 41 villagers who were examined, and environmental samples were taken in order to identify the source of the outbreak. Culture, serology, and molecular methods were used to search for Francisella tularensis in these samples. Results: A total of 8 children were diagnosed with tularemia. Th e adults and all of the other children were seronegative for tularemia. All of the patients had a history of swimming in a pool fi lled with water from a local stream, and contact with stream water was calculated to increase the risk of developing the disease 9.3-fold. Polymerase chain reaction analysis was positive in a lymph node aspirate of the index case and in the home tap water of 3 patients as well as in the spring water and stream water in the village. Francisella tularensis could not be isolated from any culture of samples. Interestingly, the waterborne tularemia outbreak aff ected only children. Conclusion: Although tularemia has been not reported from Tekirdağ Province for 74 years, the disease reemerged in the region due to the removal of hygienic measures. Th ese clues may signify that the agent had maintained its presence in the region for many years.
Koronavirüs hastalığı-2019 (COVID-19) salgını ilk olarak Çin'de başlamış olup tüm dünyaya yayılmı... more Koronavirüs hastalığı-2019 (COVID-19) salgını ilk olarak Çin'de başlamış olup tüm dünyaya yayılmıştır. Ülkemizde salgın yönetimi Sağlık Bakanlığı koordinesinde yapılmaktadır. İlimizdeki olgu artış hızının aylar içinde değişmesiyle birlikte hastanemizde oluşan ihtiyaçlara tıbbi mikrobiyoloji laboratuvarı olarak eksiksiz cevap verilmiştir. Bu çalışmada bu deneyimlerimizi aktarmak ve pandeminin ilk yedi ayında koronavirüs laboratuvarına gelen örneklerin retrospektif analizini yapmak amaçlanmıştır. Gereç ve Yöntem: Pandemi sürecinde güvenli bir çalışma ortamı oluşturmak ve iş akışını organize etmek için laboratuvar içinde birtakım değişiklikler yapılıp rehber haline getirilmiştir. Ayrıca 21.03.2020-21.10.2020 tarihleri arasında koronavirüs laboratuvarına gelen Şiddetli akut solunum sendromu koronavirüs-2 (SARS-CoV-2) polimeraz zincir reaksiyonu (PZR) ve anti-SARS-CoV-2 antikor testleri retrospektif olarak taranmıştır. Bulgular: Laboratuvarımızda toplam 73.773 SARS-CoV-2 PZR testi, 1.170 anti SARS-CoV-2 antikor testi çalışılmıştır. Toplam PZR pozitifliğinin %2,7, antikor pozitifliğinin ise %1,9 olduğu tespit edilmiştir. Sonuç: PZR pozitiflik oranları incelendiğinde alınan önlemler ile ilk aydan sonra dramatik bir düşük gözlenmekte ve bu düşüş beşinci aya kadar devam etmektedir. Önlemlerin gevşetilmesi ile altıncı ve yedinci aylarda PZR pozitifliğinin tekrar yükseldiği görülmektedir. Pandeminin ilk aylarında elde ettiğimiz deneyimlerimizin diğer laboratuvarlar için değerli bilgiler sağlayabileceği düşünülmektedir.
Amac: Bu calismada Edirne Merkez Ilkogretim Okullari ogrencilerinde pedikulus humanus kapitis ve ... more Amac: Bu calismada Edirne Merkez Ilkogretim Okullari ogrencilerinde pedikulus humanus kapitis ve tinea kapitisin gorulme sikligi ve tinea kapitis yapan etkenlerin saptanmasi amaclandi. Olgular ve Yontemler: 2003 yili Nisan ve Mayis aylarinda Edirne merkezindeki 34 ilkogretim okulunda 12.868 ogrenci tarandi. Sac ve sacli deride lezyon gorulenlerin lezyonlu bolgelerinden, sac teli ve sacli derilerinden kazinti ornegi alindi. Alinan orneklerden KOH ile direkt mikroskobik inceleme ve sikloheksimidli Sabouraud dekstroz agarda mantar kulturu yapildi. Bulgular: Toplam 698 (%5.4) ogrencinin basinda pedikulusun sirke, nimf veya olgun sekli saptandi. Kiz ogrencilerdeki pozitiflik (%10.3) erkek ogrencilerdekinden (%0.9) daha yuksekti (p < 0.001). Enfestasyon orani en az yedi yas altinda (%3.4) bulundu (p < 0.001). Sosyo-ekonomik duzeyi dusuk ailelerin bulundugu bolgelerde bitlenme orani daha yuksekti (p < 0.001). Hicbir ogrencide tinea kapitise rastlanmadi. Sonuc: Pedikulus humanus ka...
Lysinibacillus massiliensis is an aerobic, endospore-forming, gram-negative staining bacterium wi... more Lysinibacillus massiliensis is an aerobic, endospore-forming, gram-negative staining bacterium with peritrichous flagella belonging to the Bacillaceae family. A few cases of L. massiliensis isolated from the cerebrospinal fluid and tissue have been reported. In this study, we aimed to describe a case of L. massiliensis isolated from the synovial fluid. The synovial fluid from a 74-year-old female patient was inoculated into blood culture bottle. Gram-negative rods were observed in a gram-stained smear from a positive blood culture bottle. The bacterium was identified as Lysinibacillus sphaericus/Lysinibacillus fusiformis, with a probability of 89% using an automated bacterial identification system (VITEK2; Biomerieux, France). Subsequently, 16S rRNA gene sequencing was performed, and the sequence was analyzed using the Basic Local Alignment Search Tool. The sequence had 99.9% (1426/1427) identity with the strain L. massiliensis (GenBank ID: NR_043092.1). To our knowledge, this is th...
Candida tropicalis is the fourth leading cause of candidemia in Turkey. Although C. tropicalis is... more Candida tropicalis is the fourth leading cause of candidemia in Turkey. Although C. tropicalis isolates from 1997 to 2017 were characterized as fully susceptible to antifungals, the increasing global prevalence of azole-non-susceptible (ANS) C. tropicalis and the association between high fluconazole tolerance (HFT) and fluconazole therapeutic failure (FTF) prompted us to re-evaluate azole susceptibility of C. tropicalis in Turkey. In this study, 161 C. tropicalis blood isolates from seven clinical centers were identified by ITS rDNA sequencing, genotyped by multilocus microsatellite typing, and tested for susceptibility to five azoles, two echinocandins, and amphotericin B (AMB); antifungal resistance mechanisms were assessed by sequencing of ERG11 and FKS1 genes. The results indicated that C. tropicalis isolates, which belonged to 125 genotypes grouped into 11 clusters, were fully susceptible to echinocandins and AMB; however, 18.6% of them had the ANS phenotype but only two carried the ANS-conferring mutation (Y132F). HFT was recorded in 52 isolates, 10 of which were also ANS. Large proportions of patients infected with ANS and HFT isolates (89 and 40.7%, respectively) showed FTF. Patients infected with azole-susceptible or ANS isolates did not differ in
Colistin is a polymyxin antibiotic which is considered as one of the last line agents against inf... more Colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmid-mediated colistin resistance gene mcr-2 in 2016. The rapid and interspecies dissemination of plasmid-mediated resistance mechanisms through horizontal gene transfer, have made these genes considerably threatening. After the first reports, although mcr-1/mcr-2 producing Enterobacteriaceae isolates have been reported from many countries, there have been no reports from Turkey. Thus, the aim of this study was to investigate the presence of mcr-1/mcr-2 in clinical Enterobacteriaceae ...
Objectives: It was aimed to investigate anaplasmosis seropositivity in people exposured to tick b... more Objectives: It was aimed to investigate anaplasmosis seropositivity in people exposured to tick bite. Materials and Methods: A total of 116 individuals (89 males, 27 females; mean age 43; range 6 to 88 years) with tick bite history in rural areas of Thrace Region were included in this study. Possible risk factors were evaluated by a questionnaire. Sera obtained from volunteers were preserved in andash;70˚C deep freezer until the study is done. Antibodies against Anaplasma phagocytophilum were investigated by indirect fluorescent antibody (IFA) test in the sera. Results: Antibodies against A. phagocytophilum were positive for 29 individuals (25%). Contact with horse/donkey is defined as a risk factor for anaplasmosis seropositivity. Conclusion: Antibodies against A. phagocytophilum were found at high rates in people exposured to tick bite in rural areas of Thrace Region. Amac: Kene isirigi oykusu olan kisilerde anaplazmoz seropozitifliginin arastirilmasi amaclandi. Gerecler ve Yontemler: Trakya Bolgesi kirsal alanlarinda kene isirigi oykusu olan 116 kisi (89 erkek, 27 kadin; ort. yas 43; dagilim 6-88) calismaya alindi. Bir anket formu ile risk faktoru olabilecek durumlar sorgulandi. Gonullulerden alinan serumlar calisma yapilincaya kadar andash;70˚Ca#39;lik derin dondurucuda saklandi. Serumlarda indirekt floresan antikor (IFA) yontemi ile Anaplasma phagocytophilum antikorlari arastirildi. Bulgular: A. phagocytophilum antikorlari 29 kiside (%25) pozitif bulundu. Anaplazmoz seropozitifligi icin at/esek temasi bir risk faktoru olarak belirlendi. Sonuc: Trakya Bolgesia#39;nin kirsal alanlarinda yasayan, kene ile isirilmis insanlarda A. phagocytophiluma#39;a karsi gelisen antikorlar yuksek oranlarda saptanmistir.
Tularemia is caused by Francisella tularensis which is a zoonosis frequently fatal in animals suc... more Tularemia is caused by Francisella tularensis which is a zoonosis frequently fatal in animals such as rats, guinea pigs, rabbits etc., but rarely fatal in humans (1). This disease may occur as an epidemic or can be seen sporadically. The biggest epidemic defined in Turkey started in the vicinity of Bursa in 1988, then other cases were reported from the Black Sea Region, Ankara, and more recently from Kocaeli and Konya (2). The first outbreak of tularemia occurred in the European part of Turkey (3, 4). This outbreak area included 34 villages of Kırklareli and Tekirda¤ Provinces in 1936 as well as Lüleburgaz, a district of Kırklareli. A second outbreak reemerged also in Lüleburgaz in 1945. A total of 150 and 18 patients were reported in the first and second outbreaks, respectively (3-5). After that time, tularemia has not been reported in this region. We present here the first case of tularemia diagnosed in 60 years.
Induced molting is a management tool to prepare laying hens for a second reproduction period. In ... more Induced molting is a management tool to prepare laying hens for a second reproduction period. In commercial layer flocks, aged and unprofitable hens are either molted to obtain heavier, high-quality, marketable eggs [1] or production is continued by pullet replacement,
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