Papers by Stefano La Malfa
HortScience, 1998
Plant regeneration was obtained from adventitious buds induced in isolated cotyledons of Italian ... more Plant regeneration was obtained from adventitious buds induced in isolated cotyledons of Italian stone pine (Pinus pinea L.). The best results for bud induction were obtained by using half-strength LePoivre medium with 4.5 μM 6-benzyladenine for 30 days. Shoot elongation was achieved in the same medium without growth regulators but with the addition of 0.5% activated charcoal. The induction medium was the best also for shoot multiplication, but it was necessary to include subcultures on elongation medium. The slow elongation rate of adventitious shoots remains the greatest obstacle to multiplication. Root formation (15%) after 5 months was observed when shoots were cultured on elongation medium for long periods.
Physiological and Molecular Plant Pathology, 2001
Agrobacterium tumefaciens strain A281 carrying pTiBo542 was described as able to induce large and... more Agrobacterium tumefaciens strain A281 carrying pTiBo542 was described as able to induce large and earlyappearing tumours on a wider range of plants than other Agrobacterium strains. Here we show a strict correlation between the supervirulence of A281 and super-transformation ability of its disarmed derivative EHA105/p35SGUSINT. This strain produced much higher transformation than the disarmed derivative of the common strain C58, called C58(pMP90)/p35SGUSINT. Transformation frequency increased from six to 22-fold, depending on the citrus genotype. This increase permitted to regenerate for the ®rst time, whole transgenic plants from lemon, alemow and Cleopatra mandarin. Since the chromosomal background from C58 as well as the T-DNA from p35SGUSINT are identical for both bacterial strains, the super-transformation ability of EHA105/p35SGUSINT in citrus may be attributed to the vir region of pTiBo542. In addition, introduction of extra copies of virG from pTiBo542 into either EHA105/ p35SGUSINT or C58(pMP90)/p35SGUSINT further increased transformation, demonstrating the importance of vir induction, and mainly virG activation, in the super-transformation ability of this Ti plasmid in citrus.
Horticulturae
Together with lime, lemon is recognized as the third most extensively cultivated citrus species w... more Together with lime, lemon is recognized as the third most extensively cultivated citrus species worldwide, trailing only behind orange and mandarin. The enhancement in the economic value of lemons as a valuable resource in Southern Italy depends by the competitiveness of local lemon production in both domestic and international markets. This necessitates conducting a comprehensive study that thoroughly explores the available indigenous germplasm at the local and national levels. This study aims to identify the most promising Sicilian and Campanian cultivars that are well suited for both fresh fruit consumption and the production of processed goods. This re-study assumes even greater importance considering the ongoing climate changes, as environmental stresses significantly impact the ripening process and the timing of fruit development. Our study has highlighted a notable diversity among the 18 investigated lemon cultivars, particularly highlighting specific cultivars that possess d...
Rivista di frutticoltura e di ortofloricoltura, 2007
... (Depositato il 16 Giugno 2008); Antonacci, Donato e Liuni, Serana e Caputo, Angelo Raffaele e... more ... (Depositato il 16 Giugno 2008); Antonacci, Donato e Liuni, Serana e Caputo, Angelo Raffaele e Storelli, Leonardo e Lamacchia, Giuseppe e Zecca, Francesco . ... (Depositato il 16 Giugno 2008); Dimartino Aleppo, Emanuela e Perri, Francesco e Battiato, Angelo e Calandra ...
... The toxin activation of these genes was also detected in other Citrus genotypes and in leaf t... more ... The toxin activation of these genes was also detected in other Citrus genotypes and in leaf tissues ... Applicando le tecniche DDRT-PCR e PCR-Selected cDNA subtraction al binomio limone - Phoma tracheiphila, e' stato possibile identificare 15 geni diversi che sono stati attivati in ...
Tree Genetics & Genomes, Nov 9, 2021
Lemon (Citrus limon (L.) Burm. f.) is an evergreen tree belonging to the genus Citrus. The fruits... more Lemon (Citrus limon (L.) Burm. f.) is an evergreen tree belonging to the genus Citrus. The fruits are particularly prized for their organoleptic and nutraceutical properties of the juice. Herein we report, for the first time, the release of a high-quality reference genome of the two haplotypes of lemon. The sequencing has been carried out coupling Illumina short reads and Oxford Nanopore data leading to the definition of a primary and an alternative assembly characterized by a genome size of 312.8 Mb and 324.74 Mb respectively. The analysis of the long terminal repeat (LTR) allowed the identification of 1921 regions on the primary and 1911 on the alternative assembly distributed across the nine chromosomes. Furthermore, an in-silico analysis of the microRNA genes was carried out using 246 mature miRNA and the respective pre-miRNA hairpin sequences of C. sinensis. Such analysis highlighted a high conservation between the two species with 233 mature miRNAs and 51 pre-miRNA stem-loops aligning with perfect match on the lemon genome. In parallel, total RNA was extracted from fruit, flower, leaf and root enabling the detection of 38,205 and 37,753 predicted transcripts on primary and alternative assemblies respectively. Among those, the highest and lowest number of tissue-specific transcripts were detected in flower (2.73% and 2.71% in primary and alternative assemblies respectively) and leaf (0.7% and 0.68%) while gene ontology analysis enables a more precise characterization of the expressed genes based on their function. The availability of a reference genome is an important prerequisite both for the set-up of high-throughput genotyping analysis and for functional genomic approaches toward the characterization of the genetic determinism of traits of agronomic interest.
Scientia Horticulturae, Dec 1, 2015
Abstract The genus Prunus taxonomy has been widely studied using different molecular markers. Amo... more Abstract The genus Prunus taxonomy has been widely studied using different molecular markers. Among the several markers used for studying the genetic variability on Prunus, extranuclear DNA analysis is a useful tool for population and evolutionary biology studies. High resolution melting (HRM), considered a powerful method to easily detect genetic differences among sequences, was used to analyse a group of 22 genotypes belonging to 10 Prunus species using twelve newly designed chloroplastic single nucleotide variants (SNVs), ten chloroplastic SSRs, and seven universal mitochondrial markers already available in literature. The analysis allowed the investigation of the predicted and newly discovered SNVs and the detection of more alleles because of the variability found in the sequences flanking cpSSR repeat motifs. Overall, five newly discovered chloroplastic markers, four cpSSRs, and three mitochondrial markers, referring to nine loci, allowed the identification of two indels, 37 SNVs, and three repeat motif variation. The results allowed the unambiguous separation of Prunus genotypes at the species as well as at the cultivar level, confirming the discrimination power of the HRM derived markers and previous results on the phylogenetic relationships within the Prunus genus. The developed panel of SNVs and SSR cytoplasmic markers can be employed to identify different varieties and hybrids using the HRM approach without the requirement of the post-PCR procedures required in traditional molecular markers analysis.
Journal of Agricultural and Food Chemistry, Feb 25, 2020
The development of GM crops speeds up the obtainment of novel varieties with improved agronomic c... more The development of GM crops speeds up the obtainment of novel varieties with improved agronomic characteristics. However, risk evaluation of the use of GMs is mandatory before their release in the market. In this paper, an untargeted and comprehensive NMR-based metabolomic study was carried out on peel and flesh of a transgenic lemon clone (E23) expressing chit42 gene and exhibiting an increased tolerance to some pathogenic fungi, and on its wild type. Results highlighted a substantial equivalence of the metabolomics profile of the transgenic clone compared to the wild type. In addition, an enhanced response of the E23 clone towards fungal pathogens affecting post-harvest management in lemon was evidenced. These results confirm the potential of genetic engineering for the punctual modification of specific agronomic traits without altering the whole pattern of metabolites and open new perspectives for a more sustainable and effective management of specific postharvest diseases in citrus.
[6. Scientific meeting of Italian Horticultural Society], Spoleto, Perugia (Italy), 23-25 Apr 2002, 2002
[6. Scientific meeting of Italian Horticultural Society], Spoleto, Perugia (Italy), 23-25 Apr 2002, 2002
Rivista di frutticoltura e di ortofloricoltura, 2011
Rivista di Frutticoltura e di Ortofloricoltura (Italy), 2004
Informatore Fitopatologico, 2005
Rivista di frutticoltura e di ortofloricoltura, 2007
... STEFANO LA MALFA - MASSIMILIANO BRUGALETTA - MARCO CARUSO - ALESSANDRA GENTILE Dipartimento d... more ... STEFANO LA MALFA - MASSIMILIANO BRUGALETTA - MARCO CARUSO - ALESSANDRA GENTILE Dipartimento di OrtoFloroArboricoltura e Tecnologie Agroalimentari – Università di ... al-cune cultivar femminili ed er-mafrodite di antica diffusione nel territorio siciliano ed altre ...
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Papers by Stefano La Malfa