Papers by Regine dos Fernandes Vieira
Arquivos de Ciências do Mar, Jul 1, 2014
A avaliação da qualidade da água das praias do litoral oeste de Fortaleza é realizada neste traba... more A avaliação da qualidade da água das praias do litoral oeste de Fortaleza é realizada neste trabalho com base em amostras coletadas em seis pontos críticos, em períodos bimestrais ao longo de 12 meses, entre abril de 2010 e fevereiro de 2011. Para análise desse atributo, foram feitas determinações da concentração de coliformes totais, coliformes termotolerantes e de Escherichia coli, indicativa da intensidade do impacto ambiental por esses grupos de bactérias. Os resultados indicam que os esgotos escoados através de galerias pluviais, como parte da rede de drenagem urbana, representam o principal vetor responsável pela contaminação e comprometimento das condições de balneabilidade dessas praias. Os valores obtidos para as concentrações de coliformes totais, termotolerantes e E. coli são preocupantes, sendo necessário um plano constante de monitoramento que possibilite a identificação e eliminação das principais fontes de poluição, preservando-se a qualidade ambiental da zona costeira de Fortaleza.
Revista do Instituto de Medicina Tropical de São Paulo, 2010
Antibacterial effects of aqueous and ethanolic extracts of seeds of moringa (Moringa oleifera) an... more Antibacterial effects of aqueous and ethanolic extracts of seeds of moringa (Moringa oleifera) and pods of soursop (Annona muricata) in the concentration of 1:5 and 1:10 in volumes 50, 100, 150 and 200 µL were examined against Staphylococcus aureus, Vibrio cholerae, Escherichia coli (isolated from the organism and the aquatic environment) and Salmonella Enteritidis. Antibacterial activity (inhibition halo > 13 mm) against S. aureus, V. cholerae and E. coli isolated from the whiteleg shrimp, Litopenaeus vannmaei, was detected in aqueous and ethanolic extracts of moringa. E. coli isolated from tilapiafish, Oreochromis niloticus, was sensitive to the ethanolic extract of moringa. The aqueous extracts of soursop showed an antibacterial effect against S. aureus and V. cholerae, but the antibacterial activity by the ethanol extracts of this plant was not demonstrated.
Boletim do Centro de Pesquisa de Processamento de Alimentos, 2009
International Microbiology, Sep 1, 1998
Bacteriological analyses of seawater from three main beaches in Fortaleza, Brazil were performed ... more Bacteriological analyses of seawater from three main beaches in Fortaleza, Brazil were performed during 1997. Thirty-six samples per beach were collected for a total of 108 samples. For Meireles Beach, 44% of the samples had MPN total coliforms values of at least 1100 or over 2400/100 ml, followed by Formosa and Diários beaches showing lower counts. For fecal coliforms the highest numbers were demonstrated for Formosa, followed by Meireles and Diários beaches in this descending order: 13.0%, 11.1% and 8.3%, respectively. Escherichia coli strains were identified in 76.8% of the 108 samples. Among 295 strains of E. coli, 21 belonged to serogroups O25, O26, O91, O112, O119, O158 and O164. Strains from serogroup O26 were tested using PCR, ELISA and Vero cells to detect Verotoxins VT1 and VT2 and all strains were negative. No LT and ST, as determined by ELISA and suckling mice assays, were detected among the 295 strains. All strains of E. coli were sensitive to ampicillin, cephalothin, gentamicin, tetracycline, sulfametox-trimethoprim, chloramphenicol and ciprofloxacin. Although the E. coli strains were not toxigenic, their presence in high numbers could be of public health significance.
Revista do Instituto de Medicina Tropical de São Paulo, 2006
Between April and October 2002, thirty fortnightly collections of oysters (Crassostrea rhizophore... more Between April and October 2002, thirty fortnightly collections of oysters (Crassostrea rhizophorea) from a natural oyster bed at the Cocó River estuary in the Sabiaguaba region (Fortaleza, Ceará, Brazil) were carried out, aiming to isolate Aeromonas spp. strains. Oyster samples were submitted to the direct plating (DP) and the presence/absence (P/A) methods. Aeromonas were identified in 15 (50%) samples analyzed by the DP method and in 13 (43%) analyzed by the P/A method. A. caviae, A. eucrenophila, A. media, A. sobria, A. trota, A. veronii bv. sobria, A. veronii bv. veronii and Aeromonas sp. were isolated. The predominant species was A. veronii (both biovars), which was identified in 13 (43%) samples, followed by A. media in 11 (37%) and A. caviae in seven (23%). From the 59 strains identified, 28 (48%) presented resistance to at least one of the eight antibiotics tested.
Brazilian Journal of Microbiology, 2010
Thirty water samples were collected, at two week intervals, from the estuary of the River Cocó. T... more Thirty water samples were collected, at two week intervals, from the estuary of the River Cocó. The aim was to characterize the presence, distribution and types of Aeromonas spp, in the estuary of the River Cocó, Ceara, Brazil (03°46'28.83''S e 38°26'36.52''S). Aeromonas were identified in 19 (63%) samples analyzed by plating and CFU counts. Presence/absence tests were positive for 11 (37%) of the samples resulting in the detection of Aeromonas in a total of 23 (77%) of samples. CFU counts varied from < 10 to 1.4 x 10 4 CFU mL-1. From the isolated strains seven species of Aeromonas were identified: A. caviae (29/69), A. veronii bv. sobria (13/69), A. veronii bv. veronii (8/69), A. trota (6/69), A. media (5/69), A. sobria (4/69) and A. hydrophila and Aeromonas sp. (2/69). Of the 38 strains tested, 23 (60%) showed resistance to at least one of the eight antimicrobials. Multiple resistance to antibiotics was observed in A. caviae, A. media, A. sóbria and A. veronii bv. sobria. Aeromonas caviae showed the highest multiple resistance, being resistant to four antibiotics. The presence of those microorganisms may contribute to the occurrence of gastroenteritis, mainly in children, since they are considered opportunists.
Current Microbiology, 2011
Between October 2008 and June 2009, 15 samples of 10 live oysters each (Crassostrea rhizophorae) ... more Between October 2008 and June 2009, 15 samples of 10 live oysters each (Crassostrea rhizophorae) measuring 8.31-10.71 cm were purchased from a restaurant on the seashore of Fortaleza, Brazil. The Vibrio count ranged from 75 (estimated) to 43,500 CFU/g. Fourteen species were identified among the 56 isolated Vibrio strains, with V. parahaemolyticus as the most prevalent. Two of the 17 V. parahaemolyticus strains were urease-positive and tdh- and trh-positive on multiplex PCR, but neither produced β-hemolysis halos in Wagatsuma agar. Thus, fresh oysters served in natura in Fortaleza, Brazil, were found to contain Vibrio strains known to cause gastroenteritis in humans.
Hig. aliment, 2002
... Id: 331636. Autor: Evangelista-Barreto, Norma Suely; Vieira, Regine Helena Silva dos Fernande... more ... Id: 331636. Autor: Evangelista-Barreto, Norma Suely; Vieira, Regine Helena Silva dos Fernandes. Título: Salmonella versus manipuladores de alimentos: um fator de risco para os consumidores / Salmonella versus food handless: a risk factor to the consumers. Fonte: Hig. ...
Acta Scientiarum. Biological Sciences, 2018
Some infections caused by pathogenic microorganisms might shows high prevalence in farmed shrimp ... more Some infections caused by pathogenic microorganisms might shows high prevalence in farmed shrimp environments, compromising production and causing economic losses. Therefore, the search for compounds with antibiotic activity has become intensive, following the record of new antimicrobial-resistant bacteria. The study of those bioactive compounds in marine macroalgae has produced satisfactory results, such as the discovery of antibacterial activity against multiresistant strains. Accordingly, this study aims to research antibiotic activity in macroalgae extracts of Chlorophyta, Phaeophyta and Rhodophyta found in the coast of Ceara and also to evaluate the cytotoxicity activity against bacterial strains ( Vibrio sp.) from shrimp farms ( Litopenaeus vannamei ). The extracts cytotoxicity was also evaluated. The results prove that there was antibacterial activity in ethanolic, acetonic, hexanic and methanolic extracts against bacterial strains of Vibrio with multiple resistance profile a...
The phenotypic characterization of 100 Vibrio strains isolated from the hemolymph of cultured Pac... more The phenotypic characterization of 100 Vibrio strains isolated from the hemolymph of cultured Pacific white shrimp, Litopenaeus vannamei, was performed. This process allowed the identification of 10 species: V. navarrensis (n = 53), V. brasiliensis (n = 15), V. parahaemolyticus (n = 10), V. xuii (n = 8), V. cholerae (n = 5), V. coralliilyticus (n = 4), V. neptunis (n = 2), V. alginolyticus (n = 1), V. diazotrophicus (n = 1), and V. vulnificus B3 (n = 1). These results suggest that penaeid hemolymph may be colonized by vibrios with no imposition or necessity of pathological compromising.
This research work deals with monitoring of the water off the construction area of the Pecem harb... more This research work deals with monitoring of the water off the construction area of the Pecem harbor, Ceara State, Brazil and its influence zone, carried out in 1998. Data on such hydrological parameters as temperature and salinity indicate a slight trend of vertical variation, what is a clear sign of the environmental homogeneity of the studied region. The surface values of dissolved oxygen varied within the saturation limits, but with a downward trend with depth. pH values were in the range of 7.94 – 8.54 (alkaline), indicating the absence of acid substances. Waters have low transparency and high index of light extinction on account of a high content of solids in suspension, but no oils or grease were detected. Contamination by fecal coliforms was practically non existent.
This research work was designed to evaluate the inactivation of Vibrio cholerae by the citric and... more This research work was designed to evaluate the inactivation of Vibrio cholerae by the citric and acetic acids. In the first phase, the resistance of pure, O1 Inaba V. cholerae strains cultivated in TBS 1% was tested in contact with watery solutions of the citric and acetic acids under 0.1% to 1.0% concentrations. No viable cells of V. cholerae were retrieved under any of those tested concentrations. In the second phase samples of marine shrimps with 107 cells per ml of that bacterium were contaminated. Later, 25-g of shrimp subsamples were treated with lemon juice, red wine vinegar, and watery solutions of 4% acetic acid and 7% citric acid. After the contact time with the V. cholerae culture (15 min.) expired, CPP tests on TCBS were made. As a general conclusion, it was found out that none of the used solutions were effective for inactivating V. cholerae as shrimp contaminant.
Arquivos do Instituto Biológico
RESUMO Este trabalho objetivou caracterizar a resistência antimicrobiana de 43 cepas de Escherich... more RESUMO Este trabalho objetivou caracterizar a resistência antimicrobiana de 43 cepas de Escherichia coli isoladas do açude Santo Anastácio (Fortaleza, CE), durante os meses de janeiro a abril de 2007. As cepas foram submetidas a testes de suscetibilidade a oito antimicrobianos, tendo 67,4% delas apresentado sensibilidade a todos. Observou-se resistência à tetraciclina (25,6%), ao sulfazotrin (18,6%), ao ácido nalidixíco (9,3%) e a ciprofloxacina (4,7%). Nenhuma resistência foi observada aos betalactâmicos. Nove (21%) cepas apresentaram multirresistência, com um percentual de 78% para resistência cromossômica e 56% para resistência plasmidial. A presença de bactérias resistentes pode estar relacionada ao lançamento de antimicrobianos nas águas através de esgotos, sendo que o uso inadequado dessas águas pode comprometer a saúde da população.
BioMed research international, 2017
Prospect of antibacterial agents may provide an alternative therapy for diseases caused by multid... more Prospect of antibacterial agents may provide an alternative therapy for diseases caused by multidrug-resistant bacteria. This study aimed to evaluate the in vitro bioactivity of Moringa oleifera seed extracts against 100 vibrios isolated from the marine shrimp Litopenaeus vannamei. Ethanol extracts at low (MOS-E) and hot (MOS-ES) temperature are shown to be bioactive against 92% and 90% of the strains, respectively. The most efficient Minimum Inhibitory Concentration (MIC) levels of MOS-E and MOS-ES against a high percentage of strains were 32 µg mL(-1). Bioguided screening of bioactive compounds showed that the ethyl acetate fraction from both extracts was the only one that showed antibacterial activity. Vibriocidal substances, niazirine and niazimicine, were isolated from the aforementioned fraction through chromatographic fractionation.
Revista de Ciências Ambientais, 2017
Este trabalho teve como objetivo avaliar a capacidade de bactérias marinhas, isoladas de região p... more Este trabalho teve como objetivo avaliar a capacidade de bactérias marinhas, isoladas de região portuária de Fortaleza, Ceará, em produzir compostos biossurfactantes utilizando querosene comercial como fonte de carbono. Foram utilizadas 22 cepas bacterianas, sendo 14 provenientes de amostras de água e 8 de sedimento. Os microrganismos foram crescidos por um período de 72 horas em meio de cultura Luria Bertani (LB) tendo como única fonte de carbono o querosene na concentração de 2%. Com o sobrenandante (líquido metabólico - LM) das culturas bacterianas foram realizados os seguintes testes de avaliação da produção de biossurfactantes: colapso da gota, atividade de emulsificação, capacidade de formação e estabilidade de emulsão. Foi avaliada a capacidade dos isolados em produzir hemólise em meio Ágar Sangue, indicativo da produção de biossurfacatntes. Do total de isolados, 36% obtiveram forte atividade no teste do colapso da gota, 50% produziram boas emulsões (acima de 40%), apresentan...
Orbital - The Electronic Journal of Chemistry, 2016
Pesticides play an important role in the increase of productivity in agro-industry and the extens... more Pesticides play an important role in the increase of productivity in agro-industry and the extensive use of these substances cause environmental, economic and social damage in time. Microbial activity is an essential part in the dynamics and the destination of pesticides in the environment. This research focuses in prospecting and characterizing bacterial strains which are potentially able to degrade/tolerate Atrazine, Chlorpyrifos, Methyl parathion and Picloram. Bacteria were isolated from water samples collected according to the degree of salinity along the Pacotí River's estuary (Ceará), located in the semi-arid region of northeastern Brazil. A total of 49 bacterial strains were isolated, all of which tolerated/ downgraded concentrations up to 200mg/L of picloram, atrazine and methyl parathion. Tested in pesticide mixtures, the percentage and tolerance level showed that 73% grew in concentrations up to 200mg/L, 17,4% tolerated/ downgraded up to 150ml/L and the remainder only grew in concentrations under 100ml/L. The strains which had the best performance against pesticides, by points, were P1 (13Db e 14D); P2 (10E); P3 (2M, 9M, 10M, 12Mb, 14M, 17M 18Mp 19M e 20M). A high percentage of isolates (67%) expressed luminescence when exposed to the pesticides atrazine and methyl parathion in concentrations between 150 and 200ml/L.
Hig Aliment, Feb 1, 2009
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