Chrysanthemum blossoms, renowned for their aesthetic appeal, have gained recognition as potential... more Chrysanthemum blossoms, renowned for their aesthetic appeal, have gained recognition as potential contributors to nutraceuticals, cosmeceuticals, and pharmaceuticals, thereby impacting human health. Phytochemical composition and antioxidative properties of 22 distinct Indian chrysanthemum cultivars was studied. The cultivars viz., Bidhan Protima, Mauve Sarah, Silk Brocate, Diamond Jubilee, and Mahatma Gandhi displayed remarkable levels of phenolic compounds, tannins, saponins, and carotenoids, emphasizing their superior antioxidant capacity. This study also presents the rst quantitative determination of total saponin content in Chrysanthemum morifolium. Hierarchical analysis categorized cultivars into three groups based on their phytochemical pro les. Correlation analysis underscored the importance of anthocyanins and phenolic compounds in antioxidative attributes. Additionally, variations in carotenoid content unveiled astaxanthin, zeaxanthin, mutatochrome, canthaxanthin, neoxanthin, violaxanthin, and auroxanthin as potential biomarkers in differentiating cultivars. The research elucidates the rich phytochemical diversity of Indian chrysanthemum cultivars, positioning them as invaluable resources for nutraceuticals and pharmaceuticals, with potential bene ts for human well-being.
bioRxiv (Cold Spring Harbor Laboratory), Dec 21, 2023
The Glycine max L. has been affected by more than 100 diseases, including Macrophomina phaseolina... more The Glycine max L. has been affected by more than 100 diseases, including Macrophomina phaseolina producing charcoal rot disease, which reduces production by 70%. In this investigation, RNA-Seq analysis is used for the first time to explore role of silicon in preventing soybean charcoal rot. The study explores the molecular mechanism underlying soybeans' resilience to charcoal rot when treated with potassium silicon. It was meticulously investigated how Macrophomina phaseolina entered the roots. The SEM, which showed a strong link between potassium silicate accumulation and disease resistance. Further investigation indicates that a potassium silicate concentration of 1.7mM lowers disease incidence. Using Illumina HiSeq NGS data, we present a transcriptome analysis revealing genes associated with charcoal rot resistance, highlighting 3,106 genes with distinct expression patterns. The strong enrichment of pathways including "Biosynthesis of ansamycins" and "Flavone and flavonol biosynthesis," which contribute to resistance against charcoal rot, is highlighted by KEGG enrichment analysis. The ERF transcription factor and NB leucine-rich repeats stands out among the differentially expressed genes as being particularly connected to resistance. The crucial functions that many other important transcription factors, including as MYB, NAC, and proteins from the FAR1 family, play in enhancing soybeans' resistance to charcoal rot are also noted. This newly discovered information could help in developing tactics to strengthen soybean's resistance to Macrophomina phaseolina.
RNA interference (RNAi) is the process in which short interfering RNA (siRNA) act to inactivate t... more RNA interference (RNAi) is the process in which short interfering RNA (siRNA) act to inactivate the expression of target genes. The tremendous work done by many research groups around the globe have contributed in deciphering the RNAi pathway. Understanding the role of siRNA and machinery involved in RNAi pathway led to application of this pathway as technique in therapeutic applications and crop improvement. The specificity of siRNA in interacting the target sequence helped to understand the complex pathways and role of major genes involved. Here we have reviewed the journey involved in understanding RNAi pathway and in vitro use of dsRNA to induce RNAi machinery against the target gene. It explains the advances achieved in vector construction from simple RNA construct to high-throughput ihpRNA constructs for higher efficiency in target-specific gene silencing for crop improvement.
The plant pathogen interaction was studied in relation to phenylpropanoid pathway at biochemical ... more The plant pathogen interaction was studied in relation to phenylpropanoid pathway at biochemical level in the resistant and susceptible genotypes of castor at 0 h after infection (h.a.i), 24, 48 and 72 h.a.i, respectively, in wilt pathogen infected and non-infected genotypes. The phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), Cinnamate 4-hydroxylase (C4H), p-Coumaric acid-3-hydroxylase (C3H) activities were studied in both resistant and susceptible varieties. Higher activities of these enzymes were noted in resistant genotypes at interval of 24 h.a.i for PAL, 48 h.a.i. for PPO and C4H while 0 h.a.i for C3H. The presence of one constitutive isoform and an induced isoform was noted for PPO. Isoform PPO-2 was absent in infected and non-infected susceptible genotypes at 48 h.a.i. Thus, overall results indicated the involvement of several of the phenolics and quinones in the processes of resistance.
Chrysanthemum blossoms, renowned for their aesthetic appeal, have gained recognition as potential... more Chrysanthemum blossoms, renowned for their aesthetic appeal, have gained recognition as potential contributors to nutraceuticals, cosmeceuticals, and pharmaceuticals, thereby impacting human health. Phytochemical composition and antioxidative properties of 22 distinct Indian chrysanthemum cultivars was studied. The cultivars viz., Bidhan Protima, Mauve Sarah, Silk Brocate, Diamond Jubilee, and Mahatma Gandhi displayed remarkable levels of phenolic compounds, tannins, saponins, and carotenoids, emphasizing their superior antioxidant capacity. This study also presents the rst quantitative determination of total saponin content in Chrysanthemum morifolium. Hierarchical analysis categorized cultivars into three groups based on their phytochemical pro les. Correlation analysis underscored the importance of anthocyanins and phenolic compounds in antioxidative attributes. Additionally, variations in carotenoid content unveiled astaxanthin, zeaxanthin, mutatochrome, canthaxanthin, neoxanthin, violaxanthin, and auroxanthin as potential biomarkers in differentiating cultivars. The research elucidates the rich phytochemical diversity of Indian chrysanthemum cultivars, positioning them as invaluable resources for nutraceuticals and pharmaceuticals, with potential bene ts for human well-being.
bioRxiv (Cold Spring Harbor Laboratory), Dec 21, 2023
The Glycine max L. has been affected by more than 100 diseases, including Macrophomina phaseolina... more The Glycine max L. has been affected by more than 100 diseases, including Macrophomina phaseolina producing charcoal rot disease, which reduces production by 70%. In this investigation, RNA-Seq analysis is used for the first time to explore role of silicon in preventing soybean charcoal rot. The study explores the molecular mechanism underlying soybeans' resilience to charcoal rot when treated with potassium silicon. It was meticulously investigated how Macrophomina phaseolina entered the roots. The SEM, which showed a strong link between potassium silicate accumulation and disease resistance. Further investigation indicates that a potassium silicate concentration of 1.7mM lowers disease incidence. Using Illumina HiSeq NGS data, we present a transcriptome analysis revealing genes associated with charcoal rot resistance, highlighting 3,106 genes with distinct expression patterns. The strong enrichment of pathways including "Biosynthesis of ansamycins" and "Flavone and flavonol biosynthesis," which contribute to resistance against charcoal rot, is highlighted by KEGG enrichment analysis. The ERF transcription factor and NB leucine-rich repeats stands out among the differentially expressed genes as being particularly connected to resistance. The crucial functions that many other important transcription factors, including as MYB, NAC, and proteins from the FAR1 family, play in enhancing soybeans' resistance to charcoal rot are also noted. This newly discovered information could help in developing tactics to strengthen soybean's resistance to Macrophomina phaseolina.
RNA interference (RNAi) is the process in which short interfering RNA (siRNA) act to inactivate t... more RNA interference (RNAi) is the process in which short interfering RNA (siRNA) act to inactivate the expression of target genes. The tremendous work done by many research groups around the globe have contributed in deciphering the RNAi pathway. Understanding the role of siRNA and machinery involved in RNAi pathway led to application of this pathway as technique in therapeutic applications and crop improvement. The specificity of siRNA in interacting the target sequence helped to understand the complex pathways and role of major genes involved. Here we have reviewed the journey involved in understanding RNAi pathway and in vitro use of dsRNA to induce RNAi machinery against the target gene. It explains the advances achieved in vector construction from simple RNA construct to high-throughput ihpRNA constructs for higher efficiency in target-specific gene silencing for crop improvement.
The plant pathogen interaction was studied in relation to phenylpropanoid pathway at biochemical ... more The plant pathogen interaction was studied in relation to phenylpropanoid pathway at biochemical level in the resistant and susceptible genotypes of castor at 0 h after infection (h.a.i), 24, 48 and 72 h.a.i, respectively, in wilt pathogen infected and non-infected genotypes. The phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), Cinnamate 4-hydroxylase (C4H), p-Coumaric acid-3-hydroxylase (C3H) activities were studied in both resistant and susceptible varieties. Higher activities of these enzymes were noted in resistant genotypes at interval of 24 h.a.i for PAL, 48 h.a.i. for PPO and C4H while 0 h.a.i for C3H. The presence of one constitutive isoform and an induced isoform was noted for PPO. Isoform PPO-2 was absent in infected and non-infected susceptible genotypes at 48 h.a.i. Thus, overall results indicated the involvement of several of the phenolics and quinones in the processes of resistance.
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Papers by Pritam Jadhav