With the recent advances in regenerative medicine, nanotechnology has created a niche for itself ... more With the recent advances in regenerative medicine, nanotechnology has created a niche for itself as a promising avenue in this field. Innumerable studies have been carried out by researchers using virus-based methodologies for the purpose of epigenetic reprogramming. Although this method is ostensibly safe, nonetheless, they are tagged with the risk of viral genome integration into the host genome or insertional mutagenesis. Transient transfection by the use of nanocarriers is the best way to overcome these problems. This review focuses on some of the significant works carried out by researchers utilizing nanocarrier systems that have shown promising results and thus created a landmark in the epigenetic reprogramming.
Recombinant human gelatins with defined molecular weights were modified with cholesterol to make ... more Recombinant human gelatins with defined molecular weights were modified with cholesterol to make them amphiphilic in nature. We investigated the feasibility of these modified human gelatins acting as a carrier of antigenic proteins for inducing cellular immunity. The aim of this study was to synthesize novel and effective compounds for vaccine delivery in vivo. Two types of cholesterol-modified gelatin micelles, anionic cholesterol-modified gelatin (aCMG) and cationic-cholesterol modified gelatin (cCMG), were synthesized using different cholesterol derivatives such as the cholesterol-isocyanate (Ch-I) for aCMG and amino-modified cholesterol for cCMG. One was anionic and the other cationic, and therefore they differed in terms of their zeta potential. The aCMG and cCMG were characterized for their size, zeta potential, and in their ability to form micelles. Cytotoxicity was also evaluated. The modified human gelatins were then investigated as a carrier of antigenic proteins for induc...
Materials 6-carboxy fluorescein (FAM)-labeled siRNA directed against the firefly luciferase (sens... more Materials 6-carboxy fluorescein (FAM)-labeled siRNA directed against the firefly luciferase (sense 5ʹ FAM-GUGCGCUGCUGGUGCCAACuu, antisense 5ʹ-GUUGGCACCAGCAGCGCACuu) was purchased from Eurofins Operon (Huntsville, AL). 4, 6-Diamidino-2-phenylindole (DAPI) vectashield mounting medium was obtained from Vector Laboratories (Burlingame, CA). Sodium acetate, glycogen, Dulbecco's modified Eagle's medium (DMEM) low-glucose with Lglutamine and 4% paraformaldehyde in phosphate-buffered saline (PBS) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Lipofectamine® 2000 was obtained from Invitrogen (Carlsbad, CA). Passive lysis buffer (PLB) and luciferase assay system was purchased from Promega (Madison, WI). Bicinchoninic acid (BCA) protein assay kit was obtained from
With the recent advances in regenerative medicine, nanotechnology has created a niche for itself ... more With the recent advances in regenerative medicine, nanotechnology has created a niche for itself as a promising avenue in this field. Innumerable studies have been carried out by researchers using virus-based methodologies for the purpose of epigenetic reprogramming. Although this method is ostensibly safe, nonetheless, they are tagged with the risk of viral genome integration into the host genome or insertional mutagenesis. Transient transfection by the use of nanocarriers is the best way to overcome these problems. This review focuses on some of the significant works carried out by researchers utilizing nanocarrier systems that have shown promising results and thus created a landmark in the epigenetic reprogramming.
Recombinant human gelatins with defined molecular weights were modified with cholesterol to make ... more Recombinant human gelatins with defined molecular weights were modified with cholesterol to make them amphiphilic in nature. We investigated the feasibility of these modified human gelatins acting as a carrier of antigenic proteins for inducing cellular immunity. The aim of this study was to synthesize novel and effective compounds for vaccine delivery in vivo. Two types of cholesterol-modified gelatin micelles, anionic cholesterol-modified gelatin (aCMG) and cationic-cholesterol modified gelatin (cCMG), were synthesized using different cholesterol derivatives such as the cholesterol-isocyanate (Ch-I) for aCMG and amino-modified cholesterol for cCMG. One was anionic and the other cationic, and therefore they differed in terms of their zeta potential. The aCMG and cCMG were characterized for their size, zeta potential, and in their ability to form micelles. Cytotoxicity was also evaluated. The modified human gelatins were then investigated as a carrier of antigenic proteins for induc...
Materials 6-carboxy fluorescein (FAM)-labeled siRNA directed against the firefly luciferase (sens... more Materials 6-carboxy fluorescein (FAM)-labeled siRNA directed against the firefly luciferase (sense 5ʹ FAM-GUGCGCUGCUGGUGCCAACuu, antisense 5ʹ-GUUGGCACCAGCAGCGCACuu) was purchased from Eurofins Operon (Huntsville, AL). 4, 6-Diamidino-2-phenylindole (DAPI) vectashield mounting medium was obtained from Vector Laboratories (Burlingame, CA). Sodium acetate, glycogen, Dulbecco's modified Eagle's medium (DMEM) low-glucose with Lglutamine and 4% paraformaldehyde in phosphate-buffered saline (PBS) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Lipofectamine® 2000 was obtained from Invitrogen (Carlsbad, CA). Passive lysis buffer (PLB) and luciferase assay system was purchased from Promega (Madison, WI). Bicinchoninic acid (BCA) protein assay kit was obtained from
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