Papers by Priyanka Pachpande
IP archives of cytology and histopathology research, Oct 14, 2023
Oral carcinogenesis is complex and multi-step process, which results from various deleterious hab... more Oral carcinogenesis is complex and multi-step process, which results from various deleterious habits, multiple environmental factors and genetic susceptibility. CLDN-1 expression is regulated oncogenic Wnt/B-catenin transduction pathway. They recruit matrix metalloproteinases (MMPs) on the cell surface to achieve elevated focal concentrations and eventual activations of proMMP2. These collagenases are responsible for the breakdown of extracellular matrix proteins and thus facilitate invasion and spread of malignant cells. Reduced cell-cell adhesion is associated with loss of contact inhibition of proliferation. This allows escape from growth control signals and triggers carcinogenesis. Significant correlation was observed between histopathological grade of the tumor with the localization and immunostaining intensity of CLDN-1. Determination of localization of CLDN-1 for a particular patient may be important to decide site (cytoplasmic or nuclear) for targeting CLDN-1. This targeted drug therapy for CLDN-1 may prevent worsening of the disease in the patient, resulting in better prognosis. This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
This article aimed to collectively present the demographic, clinical, radiographic and histopatho... more This article aimed to collectively present the demographic, clinical, radiographic and histopathological features as well as the treatment performed along with its outcome for all the cases of non-calcifying Langerhans
Indian Journal of Pathology and Oncology
The detection of a white and red lesion on the oral mucosa can bring a number of obstacles to the... more The detection of a white and red lesion on the oral mucosa can bring a number of obstacles to the practitioner, since they can range from benign to malignant processes. An important group of ailments characterized by white and red patches of the oral mucosa include infections, traumatic lesions, systemic and local immune-mediated lesions, possibly cancerous disorders, and neoplasms. The oral mucosa is frequently affected with benign white lesions that don't need to be treated. These include birth defects or developmental disorders including Fordyce granules, hereditary benign intraepithelial dyskeratosis, pachyonychia congenita, and white sponge nevus.While many of the infective and inflammatory conditions for which causes are known can be treated in primary care, some persistent white and red patches, which have the potential to transform to malignancy, require a specialist's attention.Clinical diagnostic skills and good judgment forms the key to successful management of wh...
IP Archives of Cytology and Histopathology Research
OSCC is multifactorial and is a multistep process. It may arise de-novo or may be preceded by ora... more OSCC is multifactorial and is a multistep process. It may arise de-novo or may be preceded by oral potentially malignant disorders(OPMD). During malignant transformation certain molecular changes are evident at cellular level even in the absence of phenotypic changes in the tissue. With the recent advances in molecular understanding of cancers, immune checkpoint inhibitors have been recognized as perpetrators or supporters in the pathophysiology of various cancers. One of the main immunosuppressive pathways is the programmed death 1 (PD-1)/programmed death-ligand 1 (PD-L1) in which there is an interaction between T-cell PD-1 receptor and PD-L1 on cancer cells. Upregulation of PD-L1 is associated with disease progression.To assess and compare the PD-L1 expression in OPMD and OSCC.64 Paraffin embedded tissue sections of histopathologically diagnosed cases of 32 OSCC and 32 OPMD were immunohistochemically stained with PD-L1 & its membranous expression was evaluated. Descriptive statist...
Imaging Science in Dentistry, 2011
Central odontogenic fibroma is a rare odontogenic neoplasm that originates from odontogenic ectom... more Central odontogenic fibroma is a rare odontogenic neoplasm that originates from odontogenic ectomesenchyme. Here, a case of central odontogenic fibroma in a 17-year-old male is reported. Since the present case showed a multilocular radiolucency with partially ill-defined border between the right mandibular condyle and the distal root of the right mandibular third molar, differential diagnosis involved a wide range of pathosis from benign lesions like ameoloblastic fibroma and odontogenic myxoma to more aggressive lesions such as desmoplastic fibroma, juvenile aggressive fibromatosis, or fibrosarcoma. (Imaging Sci Dent 2011; 41 : 85-8
International Journal of Histopathological Interpretation, Mar 27, 2023
MM Publishers, 2023
The incidence of invasive infections caused by fungi has been increasing for the last few decades... more The incidence of invasive infections caused by fungi has been increasing for the last few decades. This is primarily due to the significant increases in the populations of at-risk patients; this includes those receiving immunosuppressive chemotherapy for various malignancies, solid organ transplant recipients and those receiving prolonged steroid treatment. New diagnostic approaches have been developed based on non-culture-based methods, which may allow early diagnosis and treatment of fungal infections. Laboratory procedures in diagnostic mycology are directed mainly toward the direct demonstration of the pathogenic fungi in clinical specimens by microscopy along with successful isolation of pathogenic fungi by using various culture techniques. They also help in the prediction of possible therapeutic outcome by determining antifungal susceptibility and can also be used in epidemiological studies by tracing the source of infection. The purpose of our review is to provide the reader with comprehensive and up-to-date information on diagnostic methods.
International Journal of Medical Science and Current Research , 2020
Adenoid cystic carcinoma (ADCC) is rare malignant tumor of secretory gland. It accounts for 10% o... more Adenoid cystic carcinoma (ADCC) is rare malignant tumor of secretory gland. It accounts for 10% of all salivary gland tumors. Minor salivary glands, particularly palate involve in about 75% cases with 25% in major salivary glands mainly the Parotid gland. The tumor is most often clinically deceptive due to its small size and slow growth, which actually overlies its extensive subclinical invasion, multiple recurrence and delayed onset of distant metastasis making the prognosis questionable, aptly referred as "wolf in sheep's clothing." Metastasis is common to regional lymph node, lung, liver and kidney. Wide local excision, with adjuvant radiotherapy, offers the best diagnostic modality. The aim of the present case series is to focus on its clinical presentation and its differential diagnosis.
International Journal of Histopathological Interpretation
We report a case of unicystic ameloblastoma with mucous cell differentiation in the anterior maxi... more We report a case of unicystic ameloblastoma with mucous cell differentiation in the anterior maxilla who suffered from painful swelling for 2 months. A radiograph showed a irregular radiolucent lesion between the roots of 21, 22 and 23. Microscopic examination revealed the cystic lesion was lined with ameloblastic epithelium and goblet cells in the epithelium. The mucous cells reacted positively to PAS stain. The possible pathogenic mechanism of this case reflects the pluripotential character of the odontogenic epithelium. The prognosis is probably that expected for conventional unicystic ameloblastoma. Significance of identifying such case of mucous metaplasia in unicystic ameloblastoma is that it should be differentiated from mucous cell containing lesions like glandular odontogenic cyst and mucoepidermoid carcinoma.
Journal of Oral Medicine, Oral Surgery, Oral Pathology and Oral Radiology
Diagnosis of initial epithelial pathology maybe difficult in Oral Squamous Cell Carcinoma (OSCC),... more Diagnosis of initial epithelial pathology maybe difficult in Oral Squamous Cell Carcinoma (OSCC), early invasive squamous cell carcinoma and other atypical epithelial malignancies, under routine Haematoxylin and Eosin (H and E) stain. In this context, an appropriate differential stain capable of distinguishing epithelial cells in the connective tissue may well prove to be a valuable aid. In the present study, we endeavor to use Modified Cajal's trichrome stain for making diagnosis easier without resorting to expensive diagnostic aids. Aim of present study is to assess both the epithelial and connective tissue elements in early invasive carcinoma, verrucous carcinoma and oral squamous cell carcinoma in tissue sections stained with modified Cajal's trichrome stain (MCTS) and to compare it with H and E-stained sections. Formalin-fixed, paraffin-embedded tissue blocks of early invasive SCC (n = 10) Verrucous carcinoma (n = 10) OSCC (n = 10) were stained with MCTS and H&E. The se...
International Journal of Histopathological Interpretation
We report a case of unicystic ameloblastoma with mucous cell differentiation in the anterior maxi... more We report a case of unicystic ameloblastoma with mucous cell differentiation in the anterior maxilla who suffered from painful swelling for 2 months. A radiograph showed a irregular radiolucent lesion between the roots of 21, 22 and 23. Microscopic examination revealed the cystic lesion was lined with ameloblastic epithelium and goblet cells in the epithelium. The mucous cells reacted positively to PAS stain. The possible pathogenic mechanism of this case reflects the pluripotential character of the odontogenic epithelium. The prognosis is probably that expected for conventional unicystic ameloblastoma. Significance of identifying such case of mucous metaplasia in unicystic ameloblastoma is that it should be differentiated from mucous cell containing lesions like glandular odontogenic cyst and mucoepidermoid carcinoma.
articles by Priyanka Pachpande
Journal of Oral Medicine, Oral Surgery, Oral Pathology and Oral Radiology, 2023
Introduction: Diagnosis of initial epithelial pathology maybe difficult in Oral Squamous Cell Car... more Introduction: Diagnosis of initial epithelial pathology maybe difficult in Oral Squamous Cell Carcinoma (OSCC), early invasive squamous cell carcinoma and other atypical epithelial malignancies, under routine Haematoxylin and Eosin (H and E) stain. In this context, an appropriate differential stain capable of distinguishing epithelial cells in the connective tissue may well prove to be a valuable aid. In the present study, we endeavor to use Modified Cajal's trichrome stain for making diagnosis easier without resorting to expensive diagnostic aids. Aim of present study is to assess both the epithelial and connective tissue elements in early invasive carcinoma, verrucous carcinoma and oral squamous cell carcinoma in tissue sections stained with modified Cajal's trichrome stain (MCTS) and to compare it with H and E-stained sections.
Materials and Methods: Formalin-fixed, paraffin-embedded tissue blocks of early invasive SCC (n = 10) Verrucous carcinoma (n = 10) OSCC (n = 10) were stained with MCTS and H&E. The sections were compared based on set histopathological criteria.
Results: In SCC cases stained with MCTS, invasion into connective tissue and keratin pearls were strikingly evident. Depth of invasion could be more accurately determined. Thus, CTS is a good differential stain, clearly delineating the epithelial elements from the connective tissue elements visually.
Keywords: Modified Cajal's stain, Microinvasion, H&E Stain.
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Papers by Priyanka Pachpande
articles by Priyanka Pachpande
Materials and Methods: Formalin-fixed, paraffin-embedded tissue blocks of early invasive SCC (n = 10) Verrucous carcinoma (n = 10) OSCC (n = 10) were stained with MCTS and H&E. The sections were compared based on set histopathological criteria.
Results: In SCC cases stained with MCTS, invasion into connective tissue and keratin pearls were strikingly evident. Depth of invasion could be more accurately determined. Thus, CTS is a good differential stain, clearly delineating the epithelial elements from the connective tissue elements visually.
Keywords: Modified Cajal's stain, Microinvasion, H&E Stain.
Materials and Methods: Formalin-fixed, paraffin-embedded tissue blocks of early invasive SCC (n = 10) Verrucous carcinoma (n = 10) OSCC (n = 10) were stained with MCTS and H&E. The sections were compared based on set histopathological criteria.
Results: In SCC cases stained with MCTS, invasion into connective tissue and keratin pearls were strikingly evident. Depth of invasion could be more accurately determined. Thus, CTS is a good differential stain, clearly delineating the epithelial elements from the connective tissue elements visually.
Keywords: Modified Cajal's stain, Microinvasion, H&E Stain.