An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet bee... more An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet been published. A single right arteria coronaria was found having an only left branch with a thin vena-like wall, descending on the anterior surface of the heart. The lumen of the sclerotic right arteria was occluded by a thrombus at the point of origin of ramus descendens posterior. The cause of death was an acute cardiac failure, in which authors prescribe an important part to the anomaly of coronary arteries. Areas of myocard getting blood supply from the occluded arteria were investigated by the method of fuchsinorrhagia. Authors believe, that this reaction is suitable for the demonstration of the acute, early ishaemic lesions of the myocard.
Journal of the American Society of Nephrology : JASN, Jan 7, 2016
Mechanisms of renal ischemia-reperfusion injury remain unresolved, and effective therapies are la... more Mechanisms of renal ischemia-reperfusion injury remain unresolved, and effective therapies are lacking. We previously showed that dehydroepiandrosterone protects against renal ischemia-reperfusion injury in male rats. Here, we investigated the potential role ofσ1-receptor activation in mediating this protection. In rats, pretreatment with either dehydroepiandrosterone or fluvoxamine, a high-affinityσ1-receptor agonist, improved survival, renal function and structure, and the inflammatory response after sublethal renal ischemia-reperfusion injury. In human proximal tubular epithelial cells, stimulation by fluvoxamine or oxidative stress caused theσ1-receptor to translocate from the endoplasmic reticulum to the cytosol and nucleus. Fluvoxamine stimulation in these cells also activated nitric oxide production that was blocked byσ1-receptor knockdown or Akt inhibition. Similarly, in the postischemic rat kidney,σ1-receptor activation by fluvoxamine triggered the Akt-nitric oxide synthase...
Several studies reported sexual dimorphism in the signaling mechanisms of renal ischemia/reperfus... more Several studies reported sexual dimorphism in the signaling mechanisms of renal ischemia/reperfusion (I/R). The anti-apoptotic serum and glucocorticoidregulated kinase-1 (SGK-1) is up-regulated and has a significant protective role in renal I/R. SGK-1 has several target molecules, and inhibition of the inducible nitric oxide synthase (iNOS) transcription is one of its effector mechanisms. The objective of the present study was to examine if there is a genderspecific expression and activation of SGK-1 during renal I/R injury. In vitro, treatment of HK-2 kidney proximal tubular cells with different concentrations of 17-beta estradiol had no effect, whereas testosterone increased SGK-1 abundance in a dose-dependent manner. In vivo, in a rat model of unilateral renal I/R injury, there was a higher SGK-1 expression and phosphorylation in males 2 and 24 h after ischemia paralleled by reduction in the mRNA expression of iNOS compared to females. Deprivation of testosterone by castration of males resulted in decreased SGK-1 protein level at all time-points and reduced phosphorylation 2 and 24 h after reperfusion. Our results suggest that testosterone up-regulates SGK-1 in the kidney contributing to sexual dimorphisms in the cell signalling machinery. The significance of the testosterone-regulated SGK-1 level and activity in the kidney needs further investigations.
An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet bee... more An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet been published. A single right arteria coronaria was found having an only left branch with a thin vena-like wall, descending on the anterior surface of the heart. The lumen of the sclerotic right arteria was occluded by a thrombus at the point of origin of ramus descendens posterior. The cause of death was an acute cardiac failure, in which authors prescribe an important part to the anomaly of coronary arteries. Areas of myocard getting blood supply from the occluded arteria were investigated by the method of fuchsinorrhagia. Authors believe, that this reaction is suitable for the demonstration of the acute, early ishaemic lesions of the myocard.
ACE gene insertion/deletion (I/D) polymorphism is a well-known risk factor of hypertension, cardi... more ACE gene insertion/deletion (I/D) polymorphism is a well-known risk factor of hypertension, cardiovascular diseases and progression of diabetic nephropathy. In carriers of allele D, serum level of angiotensin-II is higher, which can be associated with increased oxidative stress and subsequent endothelial damage. Albuminuria is a sensitive marker of endothelial damage, while serum activity of the enzyme gamma-glutamyl transferase--that plays important role in the antioxidant defense--may refer to the level of oxidative stress. The present paper reports on a cross-sectional clinical study, where authors have examined on the relation between ACE gene insertion/deletion polymorphism and carbohydrate metabolism, hypertension as well as albuminuria in type 2 diabetics (n = 145). In patients carrying allele D, fructosamine levels were significantly higher (p = 0.007) than in carriers of allele I. Patients with II + ID genotypes and those who were treated with insulin took more antihyperten...
The authors review the case of a 30 years old female patient presenting with a 48 hours-standing ... more The authors review the case of a 30 years old female patient presenting with a 48 hours-standing anuria, who permanently used products of grist of a virtuous plant, Guarana and occasionally used a parenteral non-steroid painkiller. The clinical history and laboratory results showed acute renal and hepatic failure. The histological picture of the renal biopsy specimen verified an acute tubular necrosis. After temporary dialysis treatment, her renal function recovered progressively with compensatory polyuria. The authors would like to draw the attention to the risks of the use of over-the-counter marketed paramedicinal products—per se or in combination with pharmaceutically registered products—sold in pharmacies and nutrition supplement stores.
An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet bee... more An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet been published. A single right arteria coronaria was found having an only left branch with a thin vena-like wall, descending on the anterior surface of the heart. The lumen of the sclerotic right arteria was occluded by a thrombus at the point of origin of ramus descendens posterior. The cause of death was an acute cardiac failure, in which authors prescribe an important part to the anomaly of coronary arteries. Areas of myocard getting blood supply from the occluded arteria were investigated by the method of fuchsinorrhagia. Authors believe, that this reaction is suitable for the demonstration of the acute, early ishaemic lesions of the myocard.
Journal of the American Society of Nephrology : JASN, Jan 7, 2016
Mechanisms of renal ischemia-reperfusion injury remain unresolved, and effective therapies are la... more Mechanisms of renal ischemia-reperfusion injury remain unresolved, and effective therapies are lacking. We previously showed that dehydroepiandrosterone protects against renal ischemia-reperfusion injury in male rats. Here, we investigated the potential role ofσ1-receptor activation in mediating this protection. In rats, pretreatment with either dehydroepiandrosterone or fluvoxamine, a high-affinityσ1-receptor agonist, improved survival, renal function and structure, and the inflammatory response after sublethal renal ischemia-reperfusion injury. In human proximal tubular epithelial cells, stimulation by fluvoxamine or oxidative stress caused theσ1-receptor to translocate from the endoplasmic reticulum to the cytosol and nucleus. Fluvoxamine stimulation in these cells also activated nitric oxide production that was blocked byσ1-receptor knockdown or Akt inhibition. Similarly, in the postischemic rat kidney,σ1-receptor activation by fluvoxamine triggered the Akt-nitric oxide synthase...
Several studies reported sexual dimorphism in the signaling mechanisms of renal ischemia/reperfus... more Several studies reported sexual dimorphism in the signaling mechanisms of renal ischemia/reperfusion (I/R). The anti-apoptotic serum and glucocorticoidregulated kinase-1 (SGK-1) is up-regulated and has a significant protective role in renal I/R. SGK-1 has several target molecules, and inhibition of the inducible nitric oxide synthase (iNOS) transcription is one of its effector mechanisms. The objective of the present study was to examine if there is a genderspecific expression and activation of SGK-1 during renal I/R injury. In vitro, treatment of HK-2 kidney proximal tubular cells with different concentrations of 17-beta estradiol had no effect, whereas testosterone increased SGK-1 abundance in a dose-dependent manner. In vivo, in a rat model of unilateral renal I/R injury, there was a higher SGK-1 expression and phosphorylation in males 2 and 24 h after ischemia paralleled by reduction in the mRNA expression of iNOS compared to females. Deprivation of testosterone by castration of males resulted in decreased SGK-1 protein level at all time-points and reduced phosphorylation 2 and 24 h after reperfusion. Our results suggest that testosterone up-regulates SGK-1 in the kidney contributing to sexual dimorphisms in the cell signalling machinery. The significance of the testosterone-regulated SGK-1 level and activity in the kidney needs further investigations.
An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet bee... more An uncommon form of anomaly of coronary arteries is reported, similar to which had not as yet been published. A single right arteria coronaria was found having an only left branch with a thin vena-like wall, descending on the anterior surface of the heart. The lumen of the sclerotic right arteria was occluded by a thrombus at the point of origin of ramus descendens posterior. The cause of death was an acute cardiac failure, in which authors prescribe an important part to the anomaly of coronary arteries. Areas of myocard getting blood supply from the occluded arteria were investigated by the method of fuchsinorrhagia. Authors believe, that this reaction is suitable for the demonstration of the acute, early ishaemic lesions of the myocard.
ACE gene insertion/deletion (I/D) polymorphism is a well-known risk factor of hypertension, cardi... more ACE gene insertion/deletion (I/D) polymorphism is a well-known risk factor of hypertension, cardiovascular diseases and progression of diabetic nephropathy. In carriers of allele D, serum level of angiotensin-II is higher, which can be associated with increased oxidative stress and subsequent endothelial damage. Albuminuria is a sensitive marker of endothelial damage, while serum activity of the enzyme gamma-glutamyl transferase--that plays important role in the antioxidant defense--may refer to the level of oxidative stress. The present paper reports on a cross-sectional clinical study, where authors have examined on the relation between ACE gene insertion/deletion polymorphism and carbohydrate metabolism, hypertension as well as albuminuria in type 2 diabetics (n = 145). In patients carrying allele D, fructosamine levels were significantly higher (p = 0.007) than in carriers of allele I. Patients with II + ID genotypes and those who were treated with insulin took more antihyperten...
The authors review the case of a 30 years old female patient presenting with a 48 hours-standing ... more The authors review the case of a 30 years old female patient presenting with a 48 hours-standing anuria, who permanently used products of grist of a virtuous plant, Guarana and occasionally used a parenteral non-steroid painkiller. The clinical history and laboratory results showed acute renal and hepatic failure. The histological picture of the renal biopsy specimen verified an acute tubular necrosis. After temporary dialysis treatment, her renal function recovered progressively with compensatory polyuria. The authors would like to draw the attention to the risks of the use of over-the-counter marketed paramedicinal products—per se or in combination with pharmaceutically registered products—sold in pharmacies and nutrition supplement stores.
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Papers by P. Degrell