Comparative biochemistry and physiology. A, Comparative physiology, 1986
Electrical parameters and transepithelial Na+ and galactose transport were determined in vitro ac... more Electrical parameters and transepithelial Na+ and galactose transport were determined in vitro across isolated duodenum of Lacerta galloti lizard. Electrical potential difference (PD) and short-circuit current (Isc) were dependent on the presence of Na+ in the bathing solutions. PD and Isc were affected by addition of galactose to the mucosal solution. Isotopic flux of Na+ measurements across short-circuited duodenum showed a net active Na+ absorption. The net flux of Na+ (JNa+net) accounted for the observed Isc. Both (JNa+net) and Isc were increased by the addition of galactose 5 mM to the mucosal solution. Isotopic flux galactose measurements in open-circuit conditions showed a net active galactose absorption. The net transport of galactose was decreased to zero in the absence of Na+ in mucosal and serosal reservoirs. Galactose has been used to induce changes in short-circuit current (delta Isc) across intestine. delta Isc was a hyperbolic function of galactose concentration chara...
Comparative Biochemistry and Physiology Part A: Physiology, 1989
Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the elec... more Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the electrophysiology and Na-Cl-transport in upper and lower intestine of the cultured fish, Sparus aurafa, were measured.
A feeding experiment was conducted to investigate the influence of eicosapentaenoic acid (20:5n-3... more A feeding experiment was conducted to investigate the influence of eicosapentaenoic acid (20:5n-3; EPA) to docosahexaenoic acid (22:6n-3; DHA) ratio (EPA/DHA) of dietary lipids on growth and fatty acid composition of gilthead seabream juveniles. Fish were fed three diets containing the same amount of total n-3 highly unsaturated fatty acids (n-3 HUFA) but different EPA/DHA ratios. No mortality was observed during the feeding trial, but significant differences were found in growth rate. The best growth rate and lowest hepatosomatic index was obtained in fish fed a diet containing an EPA/DHA ratio of 2/l. The dietary lipid treatment had no effect on the EPA/DHA ratio in neutral lipid of all the tissues studied. However, marked differences in EPA/DHA ratio were found in polar lipids of the liver, muscle and gills. It is concluded that, under the conditions employed, an EPA/DHA ratio of 2/l in the diet improves the condition of juvenile gilthead seabream. 0 1997 Elsevier Science B.V.
The fatty acid composition of ovaries from wild white sea bream and ovaries and eggs of captive w... more The fatty acid composition of ovaries from wild white sea bream and ovaries and eggs of captive white sea bream were investigated to estimate the fatty acid requirements of this species. The total lipid (TL) content in wild fish ovaries was similar to that found in ovaries and eggs ...
Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the elec... more Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the electrophysiology and Na-Cl-transport in upper and lower intestine of the cultured fish, Sparus aurafa, were measured.
Comparative biochemistry and physiology. A, Comparative physiology, 1986
Electrical parameters and transepithelial Na+ and galactose transport were determined in vitro ac... more Electrical parameters and transepithelial Na+ and galactose transport were determined in vitro across isolated duodenum of Lacerta galloti lizard. Electrical potential difference (PD) and short-circuit current (Isc) were dependent on the presence of Na+ in the bathing solutions. PD and Isc were affected by addition of galactose to the mucosal solution. Isotopic flux of Na+ measurements across short-circuited duodenum showed a net active Na+ absorption. The net flux of Na+ (JNa+net) accounted for the observed Isc. Both (JNa+net) and Isc were increased by the addition of galactose 5 mM to the mucosal solution. Isotopic flux galactose measurements in open-circuit conditions showed a net active galactose absorption. The net transport of galactose was decreased to zero in the absence of Na+ in mucosal and serosal reservoirs. Galactose has been used to induce changes in short-circuit current (delta Isc) across intestine. delta Isc was a hyperbolic function of galactose concentration chara...
Comparative Biochemistry and Physiology Part A: Physiology, 1989
Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the elec... more Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the electrophysiology and Na-Cl-transport in upper and lower intestine of the cultured fish, Sparus aurafa, were measured.
A feeding experiment was conducted to investigate the influence of eicosapentaenoic acid (20:5n-3... more A feeding experiment was conducted to investigate the influence of eicosapentaenoic acid (20:5n-3; EPA) to docosahexaenoic acid (22:6n-3; DHA) ratio (EPA/DHA) of dietary lipids on growth and fatty acid composition of gilthead seabream juveniles. Fish were fed three diets containing the same amount of total n-3 highly unsaturated fatty acids (n-3 HUFA) but different EPA/DHA ratios. No mortality was observed during the feeding trial, but significant differences were found in growth rate. The best growth rate and lowest hepatosomatic index was obtained in fish fed a diet containing an EPA/DHA ratio of 2/l. The dietary lipid treatment had no effect on the EPA/DHA ratio in neutral lipid of all the tissues studied. However, marked differences in EPA/DHA ratio were found in polar lipids of the liver, muscle and gills. It is concluded that, under the conditions employed, an EPA/DHA ratio of 2/l in the diet improves the condition of juvenile gilthead seabream. 0 1997 Elsevier Science B.V.
The fatty acid composition of ovaries from wild white sea bream and ovaries and eggs of captive w... more The fatty acid composition of ovaries from wild white sea bream and ovaries and eggs of captive white sea bream were investigated to estimate the fatty acid requirements of this species. The total lipid (TL) content in wild fish ovaries was similar to that found in ovaries and eggs ...
Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the elec... more Ahatract-1 . The influx and transepithelial movements of L-methionine and its effects on the electrophysiology and Na-Cl-transport in upper and lower intestine of the cultured fish, Sparus aurafa, were measured.
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