Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to... more Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to consider that that many cationic drugs (i.e. those characterized by the presence, at physiological pH value, of one or more cationic groups, both primary, secondary, tertiary and guanidinic aminic groups) could have any effect in impairing SARS-CoV2 entry in the host cell. This could open to new therapeutic chances against COVID-19.
A method for standardizing the antigenic activity of allergen extracts is proposed, making use of... more A method for standardizing the antigenic activity of allergen extracts is proposed, making use of a rabbit antiserum. A dilution of the latter is selected which is inhibited by the allergen extract. The antigenic activity is then evaluated by radioimmunoassay with 125I-labelled protein A from Staphylococcus aureus as tracer.
Journal of Agricultural and Food Chemistry, Aug 5, 2004
The importance of including antioxidant compounds in the diet is well recognized. These compounds... more The importance of including antioxidant compounds in the diet is well recognized. These compounds remediate the detrimental activity on animal cells of the so-called reactive oxygen substances (ROS). Many papers have reported on the determination of both hydrophilic and hydrophobic antioxidant compounds present in a large number of vegetables, and all methods involve the extraction from the matrix of the compounds to be determined. Because some problems may arise, such as the completeness of the extraction and the stability of the extracted compound during the extraction steps, the possibility of analyzing these compounds in the native matrix would be useful. Here is reported the application of near-infrared spectroscopy (NIRS) to the determination of the content of carotenoids in maize, comparing the obtained data with those derived from high-performance liquid chromatography (HPLC) determination of the extract obtained from the same samples. Equations for predicting carotenoid content in maize were derived using scores from modified partial least-squares (MPLS) as independent variables. Cross-validation procedures indicated good correlations between HPLC values and NIRS estimates. The results show that NIRS, a well-established and widely applied technique, can be applied to determine the maize carotenoids and that samples are readily analyzed in minutes, the only required step being their grinding.
... for the determination of resveratrol content in red wines / OV Brenna - In: Polyphenols Commu... more ... for the determination of resveratrol content in red wines / OV Brenna - In: Polyphenols Communications 2002[sl] : Ismail El Hadrami ... Browser supportati Firefox 3+, Internet Explorer 8+, Google Chrome, Safari ICT Support, development & maintenance are provided by the AePIC ...
Publikationsansicht. 41641379. Rice malting to obtain a gluten-free beer (2008). ... Abstract. Ri... more Publikationsansicht. 41641379. Rice malting to obtain a gluten-free beer (2008). ... Abstract. Rice malting to obtain a gluten-free beer / ELM Ceppi, OV Brenna. ((Intervento presentato al 1. International Symposium for young Scientists ...
A crude preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mucosa was purified... more A crude preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mucosa was purified by affinity chromatography on Sepharose-bound derivatives of arsanilic acid, which was found to be a competitive inhibitor of the enzyme. Three biospecific adsorbents were prepared for the chromatography, and the best results were obtained with a tyraminyl-Sepharose derivative coupled with the diazonium salt derived from 4-(p-aminophenylazo)phenylarsonic acid. Alkaline phosphatase was the only enzyme retained by the affinity column in the absence of Pi. The enzyme eluted by phosphate buffer had a specific activity of about 1200 units per mg of protein at pH 10.0, with 5.5mM-p-nitrophenyl phosphate as the substrate.
Bollettino dell'Istituto sieroterapico milanese, Jan 31, 1977
Serum C1 esterase inhibitor was determined in 138 members of 18 italian families with hereditary ... more Serum C1 esterase inhibitor was determined in 138 members of 18 italian families with hereditary angioedema by immunochemical and enzymatic assays. On the basis of quantitative and functional findings, the type A of hereditary angioedema was diagnosed in 44 subjects, and the type B in 15. Some technical devices concerning serum sample handling were identified. The influence of heparin, EDTA, and citrate on C1 esterase inhibitor activity of normal plasma was also investigated.
International Journal of Immunopathology and Pharmacology, 2020
Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to... more Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to consider that that many cationic drugs (i.e. those characterized by the presence, at physiological pH value, of one or more cationic groups, both primary, secondary, tertiary and guanidinic aminic groups) could have any effect in impairing SARS-CoV2 entry in the host cell. This could open to new therapeutic chances against COVID-19.
Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to... more Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to consider that that many cationic drugs (i.e. those characterized by the presence, at physiological pH value, of one or more cationic groups, both primary, secondary, tertiary and guanidinic aminic groups) could have any effect in impairing SARS-CoV2 entry in the host cell. This could open to new therapeutic chances against COVID-19.
A method for standardizing the antigenic activity of allergen extracts is proposed, making use of... more A method for standardizing the antigenic activity of allergen extracts is proposed, making use of a rabbit antiserum. A dilution of the latter is selected which is inhibited by the allergen extract. The antigenic activity is then evaluated by radioimmunoassay with 125I-labelled protein A from Staphylococcus aureus as tracer.
Journal of Agricultural and Food Chemistry, Aug 5, 2004
The importance of including antioxidant compounds in the diet is well recognized. These compounds... more The importance of including antioxidant compounds in the diet is well recognized. These compounds remediate the detrimental activity on animal cells of the so-called reactive oxygen substances (ROS). Many papers have reported on the determination of both hydrophilic and hydrophobic antioxidant compounds present in a large number of vegetables, and all methods involve the extraction from the matrix of the compounds to be determined. Because some problems may arise, such as the completeness of the extraction and the stability of the extracted compound during the extraction steps, the possibility of analyzing these compounds in the native matrix would be useful. Here is reported the application of near-infrared spectroscopy (NIRS) to the determination of the content of carotenoids in maize, comparing the obtained data with those derived from high-performance liquid chromatography (HPLC) determination of the extract obtained from the same samples. Equations for predicting carotenoid content in maize were derived using scores from modified partial least-squares (MPLS) as independent variables. Cross-validation procedures indicated good correlations between HPLC values and NIRS estimates. The results show that NIRS, a well-established and widely applied technique, can be applied to determine the maize carotenoids and that samples are readily analyzed in minutes, the only required step being their grinding.
... for the determination of resveratrol content in red wines / OV Brenna - In: Polyphenols Commu... more ... for the determination of resveratrol content in red wines / OV Brenna - In: Polyphenols Communications 2002[sl] : Ismail El Hadrami ... Browser supportati Firefox 3+, Internet Explorer 8+, Google Chrome, Safari ICT Support, development & maintenance are provided by the AePIC ...
Publikationsansicht. 41641379. Rice malting to obtain a gluten-free beer (2008). ... Abstract. Ri... more Publikationsansicht. 41641379. Rice malting to obtain a gluten-free beer (2008). ... Abstract. Rice malting to obtain a gluten-free beer / ELM Ceppi, OV Brenna. ((Intervento presentato al 1. International Symposium for young Scientists ...
A crude preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mucosa was purified... more A crude preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mucosa was purified by affinity chromatography on Sepharose-bound derivatives of arsanilic acid, which was found to be a competitive inhibitor of the enzyme. Three biospecific adsorbents were prepared for the chromatography, and the best results were obtained with a tyraminyl-Sepharose derivative coupled with the diazonium salt derived from 4-(p-aminophenylazo)phenylarsonic acid. Alkaline phosphatase was the only enzyme retained by the affinity column in the absence of Pi. The enzyme eluted by phosphate buffer had a specific activity of about 1200 units per mg of protein at pH 10.0, with 5.5mM-p-nitrophenyl phosphate as the substrate.
Bollettino dell'Istituto sieroterapico milanese, Jan 31, 1977
Serum C1 esterase inhibitor was determined in 138 members of 18 italian families with hereditary ... more Serum C1 esterase inhibitor was determined in 138 members of 18 italian families with hereditary angioedema by immunochemical and enzymatic assays. On the basis of quantitative and functional findings, the type A of hereditary angioedema was diagnosed in 44 subjects, and the type B in 15. Some technical devices concerning serum sample handling were identified. The influence of heparin, EDTA, and citrate on C1 esterase inhibitor activity of normal plasma was also investigated.
International Journal of Immunopathology and Pharmacology, 2020
Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to... more Given the sharp spreading of COVID-19 pandemic all around the world, our attention was brought to consider that that many cationic drugs (i.e. those characterized by the presence, at physiological pH value, of one or more cationic groups, both primary, secondary, tertiary and guanidinic aminic groups) could have any effect in impairing SARS-CoV2 entry in the host cell. This could open to new therapeutic chances against COVID-19.
Uploads
Papers by Oreste Brenna