Papers by Nazaninalsadat Seyed Khoei
Iranian Journal of Medical Hypotheses and Ideas, Sep 1, 2009
have equal contribution as first authors.
World Journal of Microbiology and Biotechnology, 2015
In natural environments, bacteria often exist in close association with surfaces and interfaces b... more In natural environments, bacteria often exist in close association with surfaces and interfaces by establishing biofilms. Here we report on the ability of Burkholderia fungorum strains DBT1 and to survive in high concentrations of hydrocarbons and compare growth as a biofilm
vs. planktonic cells. The compounds tested were dibenzothiophene (DBT) and a mixture of naphthalene, phenanthrene and pyrene (5:2:1) as representative compounds of thiophenes and polycyclic aromatic hydrocarbons (PAHs), respectively. The results showed that both strains were able to degrade dibenzothiophene and survive up to 2000 mg·L-1 of this compound both as a biofilm and as free;living cells. Moreover, B. fungorum DBT1 showed reduced tolerance towards the mixed PAHs (naphthalene 2000 mg·L-1, phenanthrene 800 mg·L-1
and pyrene 400 mg·L-1) both as a biofilm and as free;living cells. Conversely, biofilms of B. fungorum 95 enhanced resistance against these toxic compounds in comparison to planktonic cells (P < 0.05).
Visual observation through confocal laser scanning microscopy
showed that exposure of biofilms to DBT and PAHs altered their structure: high concentrations of DBT trigged an aggregation of biofilm cells. These findings provide new perspectives on the effectiveness of using DBT-degrading bacterial strains in bioremediation of hydrocarbons contaminated sites. DOI 10.1139/cjm-2016-0160.
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system. Among th... more Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system. Among the numerous proposed etiologies, Borrelia burgdorferi (a causative agent of Lyme disease) has been associated with MS. Although the current MS therapies decrease the quantity and severity of the attacks, most patients experience various neurologic symptoms obliging them to have recourse to one or more com-
The present study reports on a real case of contamination due to the chronic leakage of diesel fu... more The present study reports on a real case of contamination due to the chronic leakage of diesel fuel from an underground tank at a dismissed service station. Speciation of the microbial community according to both lateral and vertical gradients from the origin of the contaminant release was analyzed by means of the PCR–DGGE technique. Moreover, the effects of a landfarming treatment on both the microbial community structure and the abatement of contamination were analyzed. The concentration of total petrol hydrocarbons (TPHs) decreased along the horizontal gradient (from 7042.2 ± 521.9 to 112.2 ± 24.3 mg kg-1), while increased downwards from the position of the tank (from 502.6 ± 43.7 to 4972.5 ± 275.3 mg kg-1). PCR–DGGE analyses and further
statistical treatment of the data indicated a correlation between structure of the bacterial communities and amount of diesel fuel contamination. On the other hand, level of contamination, soil texture and depth were shown to affect the fungal community. Chloroflexi and Ascomycota were the most abundant microbes ascertained through cultureindependent procedures. Landfarming promoted 91.6 % reduction of TPHs in 75 days. Furthermore, PCR–DGGE analyses evidenced that both bacterial and fungal communities of the treated soil were restored to the pristine conditions of uncontaminated topsoil. The present study
demonstrated that bacterial and fungal communities were affected differently by soil factors such as level of hydrocarbon
contamination as well as soil depth and texture. This report shows that a well-planned landfarming treatment can drive the restoration of the soil in terms of both abatement of the contaminants and resilience of the microbial community structure.
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Papers by Nazaninalsadat Seyed Khoei
vs. planktonic cells. The compounds tested were dibenzothiophene (DBT) and a mixture of naphthalene, phenanthrene and pyrene (5:2:1) as representative compounds of thiophenes and polycyclic aromatic hydrocarbons (PAHs), respectively. The results showed that both strains were able to degrade dibenzothiophene and survive up to 2000 mg·L-1 of this compound both as a biofilm and as free;living cells. Moreover, B. fungorum DBT1 showed reduced tolerance towards the mixed PAHs (naphthalene 2000 mg·L-1, phenanthrene 800 mg·L-1
and pyrene 400 mg·L-1) both as a biofilm and as free;living cells. Conversely, biofilms of B. fungorum 95 enhanced resistance against these toxic compounds in comparison to planktonic cells (P < 0.05).
Visual observation through confocal laser scanning microscopy
showed that exposure of biofilms to DBT and PAHs altered their structure: high concentrations of DBT trigged an aggregation of biofilm cells. These findings provide new perspectives on the effectiveness of using DBT-degrading bacterial strains in bioremediation of hydrocarbons contaminated sites. DOI 10.1139/cjm-2016-0160.
statistical treatment of the data indicated a correlation between structure of the bacterial communities and amount of diesel fuel contamination. On the other hand, level of contamination, soil texture and depth were shown to affect the fungal community. Chloroflexi and Ascomycota were the most abundant microbes ascertained through cultureindependent procedures. Landfarming promoted 91.6 % reduction of TPHs in 75 days. Furthermore, PCR–DGGE analyses evidenced that both bacterial and fungal communities of the treated soil were restored to the pristine conditions of uncontaminated topsoil. The present study
demonstrated that bacterial and fungal communities were affected differently by soil factors such as level of hydrocarbon
contamination as well as soil depth and texture. This report shows that a well-planned landfarming treatment can drive the restoration of the soil in terms of both abatement of the contaminants and resilience of the microbial community structure.
vs. planktonic cells. The compounds tested were dibenzothiophene (DBT) and a mixture of naphthalene, phenanthrene and pyrene (5:2:1) as representative compounds of thiophenes and polycyclic aromatic hydrocarbons (PAHs), respectively. The results showed that both strains were able to degrade dibenzothiophene and survive up to 2000 mg·L-1 of this compound both as a biofilm and as free;living cells. Moreover, B. fungorum DBT1 showed reduced tolerance towards the mixed PAHs (naphthalene 2000 mg·L-1, phenanthrene 800 mg·L-1
and pyrene 400 mg·L-1) both as a biofilm and as free;living cells. Conversely, biofilms of B. fungorum 95 enhanced resistance against these toxic compounds in comparison to planktonic cells (P < 0.05).
Visual observation through confocal laser scanning microscopy
showed that exposure of biofilms to DBT and PAHs altered their structure: high concentrations of DBT trigged an aggregation of biofilm cells. These findings provide new perspectives on the effectiveness of using DBT-degrading bacterial strains in bioremediation of hydrocarbons contaminated sites. DOI 10.1139/cjm-2016-0160.
statistical treatment of the data indicated a correlation between structure of the bacterial communities and amount of diesel fuel contamination. On the other hand, level of contamination, soil texture and depth were shown to affect the fungal community. Chloroflexi and Ascomycota were the most abundant microbes ascertained through cultureindependent procedures. Landfarming promoted 91.6 % reduction of TPHs in 75 days. Furthermore, PCR–DGGE analyses evidenced that both bacterial and fungal communities of the treated soil were restored to the pristine conditions of uncontaminated topsoil. The present study
demonstrated that bacterial and fungal communities were affected differently by soil factors such as level of hydrocarbon
contamination as well as soil depth and texture. This report shows that a well-planned landfarming treatment can drive the restoration of the soil in terms of both abatement of the contaminants and resilience of the microbial community structure.