Papers by Mohamed Aboubaker
Freshwater Science, 2014
ABSTRACT on evolution of molecular diversity and the natural distribution of wild stocks of the g... more ABSTRACT on evolution of molecular diversity and the natural distribution of wild stocks of the giant freshwater prawn (Macrobrachium rosenbergii) Author(s):about/policies/terms.jsp . JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact [email protected]. .
Life Science Journal
The effects of four environmental stresses of heat, drought, wounding and cell culture, on the tr... more The effects of four environmental stresses of heat, drought, wounding and cell culture, on the transcriptional reactivation of seven long terminal repeat retrotransposons (LTRs) of barley (Hordeum vulgare) genome, were investigated. These LTRs included two Copia-type (Bare and Maximus); and five Gypsy-type (Erika, Jeli, Sabrina, Sukkula1 and Sukkula3) LTRs. RT-qPCR analyses revealed that Erika1 LTR was highly reactivated under heat, drought, and wounding with 28.1, 9.9 and 9.4 fold increments, respectively. Sabrina LTR was 6.2 fold reactivated under cell culture. Bare1 LTR was reactivated by drought (4.1 fold) and cell culture (3.4 fold). Transcription activity of Jeli LTR was increased by 3.4 fold under cell culture. Sukkula1, Sukkula3 and Maximus LTRs were slightly reactivated under drought, wounding and cell culture. These changes in the reactivation pattern of LTRs provide fingerprints for tracking the molecular changes occurred in barley genome upon exposure to environmental st...
Journal of Genetic Engineering and Biotechnology, 2015
Potato (Solanum tuberosum L.) is widely used for many industrial and food applications. Nine pota... more Potato (Solanum tuberosum L.) is widely used for many industrial and food applications. Nine potato cultivars were planted and collected from a private farm in new Salihiyyah city, Sharkia governorate, Egypt to compare between them at morphological, molecular, biochemical and anatomical levels. Our results indicated that the Inova cultivar was better, however the Bafana cultivar was worse in relation to yield parameters. Inter simple sequence repeat (ISSR) molecular marker has been used to determine the genetic diversity between these nine cultivars. Through using ten primers we obtained 98 bands, 85 of which were polymorphic by 87%. The highest similarity value (0.827) was found between Caruso and Alliance as the closest but the lowest value (0.418) was found between Charlotte and Bafana as the most distant. Everest tuber contained great amounts of total phenolic and peroxidase activity, while the Bafana tuber contained small amounts of it compared to other cultivars. The phellem layer of the Everest tuber had more thickness than others and the number of phellem rows was the highest. However, the Bafana cultivar listed the lowest value compared to other cultivars. Lower values from both of total bacterial and total fungi were recorded on the tuber of the Everest cultivar. However, Bafana cultivar was recorded to have a higher value of both compared to other cultivars. We suggest that the ISSR marker is a suitable procedure to examine the potato's genetic diversity at the DNA level. The Everest cultivar is considering the best cultivar to planting and breeding in Egypt.
Life Science Journal
The effects of four environmental stresses of heat, drought, wounding and cell culture, on the tr... more The effects of four environmental stresses of heat, drought, wounding and cell culture, on the transcriptional reactivation of seven long terminal repeat retrotransposons (LTRs) of barley (Hordeum vulgare) genome, were investigated. These LTRs included two Copia-type (Bare and Maximus); and five Gypsy-type (Erika, Jeli, Sabrina, Sukkula1 and Sukkula3) LTRs. RT-qPCR analyses revealed that Erika1 LTR was highly reactivated under heat, drought, and wounding with 28.1, 9.9 and 9.4 fold increments, respectively. Sabrina LTR was 6.2 fold reactivated under cell culture. Bare1 LTR was reactivated by drought (4.1 fold) and cell culture (3.4 fold). Transcription activity of Jeli LTR was increased by 3.4 fold under cell culture. Sukkula1, Sukkula3 and Maximus LTRs were slightly reactivated under drought, wounding and cell culture. These changes in the reactivation pattern of LTRs provide fingerprints for tracking the molecular changes occurred in barley genome upon exposure to environmental st...
Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence r... more Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence repeats (ISSRs) markers were performed to detect the genetic diversity among 6 new rice lines and 4 cultivars with different responses to drought tolerance and establish specific DNA markers associated with drought tolerance. Among 16 RAPD primers tested, only 5 produced bands polymorphic between lines with an average of 5.2 bands per primer (ranging from approximately 252 to 1232 bp) and 73.02 % were polymorphic. Among the tested ISSR primers, only five amplified polymorphic ISSR loci with an average number of 4.4 bands per primer (ranging from approximately 80 to 813 bp) and the mean percentage of ISSR polymorphism was 90.91. Based on band polymorphisms generated by RAPD-PCR and ISSR after using the primers, the highest similarity value (0.93) was found between P-5-3-b line and P-5-3-a line and the lowest value (0.44) was found between P-5-3-b line and Giza 172. The dendrogram separated all cultivars and new lines into two clusters and indicated that the cross of tolerant line (P-5-3-b) and susceptible cultivar (Giza 172) is suggested as the most suitable cross for drought tolerance analysis studies as they have the lowest similarity value (0.44) and also grouped in distinct cluster. Since two fragments of about (315 and 505 bp) were visualized using HP15 primer in the genomic DNA of the drought tolerant lines while were absent in the sensitive cultivars, they can be considered as positive drought tolerant markers. [Journal of American Science 2010;6(12):355-363]. (ISSN: 1545-1003).
Potato (Solanum tuberosum L.) is widely used for many industrial and food applications. Nine pota... more Potato (Solanum tuberosum L.) is widely used for many industrial and food applications. Nine potato cultivars were planted and collected from a private farm in new Salihiyyah city, Shar-kia governorate, Egypt to compare between them at morphological, molecular, biochemical and anatomical levels. Our results indicated that the Inova cultivar was better, however the Bafana cul-tivar was worse in relation to yield parameters. Inter simple sequence repeat (ISSR) molecular marker has been used to determine the genetic diversity between these nine cultivars. Through using ten primers we obtained 98 bands, 85 of which were polymorphic by 87%. The highest similarity value (0.827) was found between Caruso and Alliance as the closest but the lowest value (0.418) was found between Charlotte and Bafana as the most distant. Everest tuber contained great amounts of total phenolic and peroxidase activity, while the Bafana tuber contained small amounts of it compared to other cultivars. The phellem layer of the Everest tuber had more thickness than others and the number of phellem rows was the highest. However, the Bafana cultivar listed the lowest value compared to other cultivars. Lower values from both of total bacterial and total fungi were recorded on the tuber of the Everest cultivar. However, Bafana cultivar was recorded to have a higher value of both compared to other cultivars. We suggest that the ISSR marker is a suitable procedure to examine the potato's genetic diversity at the DNA level. The Everest cultivar is considering the best cultivar to planting and breeding in Egypt.
The effects of four environmental stresses of heat, drought, wounding and cell culture, on the tr... more The effects of four environmental stresses of heat, drought, wounding and cell culture, on the transcriptional reactivation of seven long terminal repeat retrotransposons (LTRs) of barley (Hordeum vulgare)
Uploads
Papers by Mohamed Aboubaker