To prospectively explore the role of carcinoembryonic antigen (CEA) in gallbladder bile in patien... more To prospectively explore the role of carcinoembryonic antigen (CEA) in gallbladder bile in patients with colorectal carcinoma and the morphological and clinical features of neoplasia and the occurrence of hepatic metastases.
Crop Breeding and Applied Biotechnology (Online), 2011
The Amazonian Tucumã palm (Astrocaryum aculeatum) produces edible fruit, traditionally consumed i... more The Amazonian Tucumã palm (Astrocaryum aculeatum) produces edible fruit, traditionally consumed in indigenous communities and increasingly in urban centers. The species is incipiently domesticated and little studied, despite its growing economic importance for smallholder farmers and gatherers. Studies on the mating system are required for the conservation and use of the species' genetic resources. Our objective was to estimate mating system parameters of the Tucumã palm using microsatellite markers. Plants of 11 progenies of a spontaneous population were genotyped with eight microsatellite loci and the mating system parameters estimated. The population outcrossing rate was estimated at 0.978, and ranged from 0.774 to 1at the family level. The estimates of the correlation of paternity (0.176 and 0.205) suggest a low probability of full-sibs within progenies. Tucumã palm is a predominantly allogamous species and the open-pollinated progenies consist predominantly of half-sibs.
A sumaúma (Ceiba pentandra L. Gaertn) é uma espécie arbórea de grande importância para o setor fl... more A sumaúma (Ceiba pentandra L. Gaertn) é uma espécie arbórea de grande importância para o setor florestal da Região Norte do Brasil. Apesar disso, ainda não existem relatos sobre o cultivo in vitro desta espécie. O presente trabalho teve como objetivo estabelecer um protocolo eficiente para assepsia e cultivo in vitro de ápices caulinares de sumaúma. Para isto foram avaliados os efeitos de diferentes agentes químicos (NaOCl, HgCl 2 e AgNO 3 ) para assepsia e estabelecimento in vitro de ápices caulinares. Diferentes tipos e concentrações de citocininas (BAP, CIN e TDZ) foram testados durante o enraizamento, alongamento e indução de brotos. Apesar das dificuldades usualmente associadas ao estabelecimento e cultivo in vitro de espécies arbóreas, os resultados obtidos não evidenciaram problemas na manipulação in vitro de sumaúma.
We isolated a cDNA from a soybean library, which encodes sucrose binding protein (SBP) homologue,... more We isolated a cDNA from a soybean library, which encodes sucrose binding protein (SBP) homologue, designated S-64. To analyze the function of the SBP homologue, transgenic tobacco plants were obtained by introducing chimeric genes containing the s-64 coding region linked to the 35S CaMV promoter, either in the sense or antisense orientation, via Agrobacterium tumefaciens-mediated transformation. The accumulation of the SBP homologue was increased in transgenic plants expressing the heterologous sbp gene, whereas those expressing the antisense construct had reduced levels of the protein. The antisense transgenic plants developed symptoms characteristic of an inhibition of sucrose translocation and displayed a reduction in plant growth and development. In contrast, overexpression of the protein accelerated plant growth and the onset of flowering induction. The overall developmental performance of the transgenic plants was correlated with their photosynthetic rate under normal conditions. While photosynthesis in the antisense lines was decreased, in the sense lines photosynthetic rates were increased. Furthermore, both antisense repression and overexpression of the SBP homologue in transgenic lines altered carbohydrate partitioning in mature leaves. Taken together, these results indicate that S-64 protein is functionally analogous to SBP, representing an important component of the sucrose translocation pathway in plants.
We have obtained Nicotiana tabacum transgenic cell lines expressing a sucrose binding protein (sb... more We have obtained Nicotiana tabacum transgenic cell lines expressing a sucrose binding protein (sbp) homologue gene from soybean (Glycine max L.), designated s-64, either in the sense or antisense orientation. Sense cell lines over-accumulated the S-64 protein, whereas the antisense cell lines had reduced levels of the endogenous homologue protein.
The sucrose binding protein (SBP) from soybean has been implicated as an important component of t... more The sucrose binding protein (SBP) from soybean has been implicated as an important component of the sucrose uptake system. Two SBP genomic clones, gsS641.1 and gsS641.2, which correspond to allelic forms of the GmSBP2/S64 gene, have been isolated and characterized. As a member of the seed storage protein superfamily, it has been shown that the SBP gene structure is similar to vicilin genes with intron/ exon boundaries at conserved positions. Fluorescence in situ hybridization (FISH) suggested that the soybean SBP gene family is represented by at least two non-allelic genes corresponding to the previously isolated GmSBP1 and GmSBP2/S64 cDNAs. These two cDNAs share extensive sequence similarity but are located at different loci in the soybean genome. To investigate transcriptional activation of the GmSBP2 gene, 2 kb 5¢-¯anking sequences of gsS641.1 and gsS641.2 were fused to the b-glucuronidase (GUS) reporter gene and to the green¯uorescent protein (GFP) reporter gene and independently introduced into Nicotiana tabacum by Agrobacterium tumefaciens-mediated transformation. The SBP2 promoter directed expression of both GUS and GFP reporter genes with high speci®city to the phloem of leaves, stems and roots. Thus, the overall pattern of SBP±GUS or SBP±GFP expression is consistent with the involvement of SBP in sucrose translocation-dependent physiological processes.
... Luis Antônio Serrão Contim I ; Carlos Roberto de Carvalho II ; Franciele Alline Martins II ; ... more ... Luis Antônio Serrão Contim I ; Carlos Roberto de Carvalho II ; Franciele Alline Martins II ; Danival Vieira de Freitas III. ... The authors thank Ms. Reginaldo AF Buzelli (Purdue University, USA) and Dr. Charles R. Clement (INPA, Brazil) for helpful discussions and critical reading of ...
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2006
The Glycine max sucrose binding protein (GmSBP2) promoter directs phloem-specific expression of r... more The Glycine max sucrose binding protein (GmSBP2) promoter directs phloem-specific expression of reporter genes in transgenic tobacco. Here, we identified cis-regulatory domains (CRD) that contribute with positive and negative regulation for the tissue-specific pattern of the GmSPB2 promoter. Negative regulatory elements in the distal CRD-A (-2000 to -700) sequences suppressed expression from the GmSBP2 promoter in tissues other than seed tissues and vascular tissues of vegetative organs. Deletion of this region relieved repression resulting in a constitutive promoter highly active in all tissues analyzed. Further deletions from the strong constitutive -700GmSBP2 promoter delimited several intercalating enhancer-like and repressing domains that function in a context-dependent manner. Histochemical examination revealed that the CRD-C (-445 to -367) harbors both negative and positive elements. This region abolished promoter expression in roots and in all tissues of stems except for the inner phloem. In contrast, it restores root meristem expression when fused to the -132pSBP2-GUS construct, which contains root meristem expression-repressing determinants mapped to the 44-bp CRD-G (-136 to -92). Thus, the GmSBP2 promoter is functionally organized into a proximal region with the combinatorial modular configuration of plant promoters and a distal domain, which restricts gene expression to the vascular tissues in vegetative organs.
To prospectively explore the role of carcinoembryonic antigen (CEA) in gallbladder bile in patien... more To prospectively explore the role of carcinoembryonic antigen (CEA) in gallbladder bile in patients with colorectal carcinoma and the morphological and clinical features of neoplasia and the occurrence of hepatic metastases.
Crop Breeding and Applied Biotechnology (Online), 2011
The Amazonian Tucumã palm (Astrocaryum aculeatum) produces edible fruit, traditionally consumed i... more The Amazonian Tucumã palm (Astrocaryum aculeatum) produces edible fruit, traditionally consumed in indigenous communities and increasingly in urban centers. The species is incipiently domesticated and little studied, despite its growing economic importance for smallholder farmers and gatherers. Studies on the mating system are required for the conservation and use of the species' genetic resources. Our objective was to estimate mating system parameters of the Tucumã palm using microsatellite markers. Plants of 11 progenies of a spontaneous population were genotyped with eight microsatellite loci and the mating system parameters estimated. The population outcrossing rate was estimated at 0.978, and ranged from 0.774 to 1at the family level. The estimates of the correlation of paternity (0.176 and 0.205) suggest a low probability of full-sibs within progenies. Tucumã palm is a predominantly allogamous species and the open-pollinated progenies consist predominantly of half-sibs.
A sumaúma (Ceiba pentandra L. Gaertn) é uma espécie arbórea de grande importância para o setor fl... more A sumaúma (Ceiba pentandra L. Gaertn) é uma espécie arbórea de grande importância para o setor florestal da Região Norte do Brasil. Apesar disso, ainda não existem relatos sobre o cultivo in vitro desta espécie. O presente trabalho teve como objetivo estabelecer um protocolo eficiente para assepsia e cultivo in vitro de ápices caulinares de sumaúma. Para isto foram avaliados os efeitos de diferentes agentes químicos (NaOCl, HgCl 2 e AgNO 3 ) para assepsia e estabelecimento in vitro de ápices caulinares. Diferentes tipos e concentrações de citocininas (BAP, CIN e TDZ) foram testados durante o enraizamento, alongamento e indução de brotos. Apesar das dificuldades usualmente associadas ao estabelecimento e cultivo in vitro de espécies arbóreas, os resultados obtidos não evidenciaram problemas na manipulação in vitro de sumaúma.
We isolated a cDNA from a soybean library, which encodes sucrose binding protein (SBP) homologue,... more We isolated a cDNA from a soybean library, which encodes sucrose binding protein (SBP) homologue, designated S-64. To analyze the function of the SBP homologue, transgenic tobacco plants were obtained by introducing chimeric genes containing the s-64 coding region linked to the 35S CaMV promoter, either in the sense or antisense orientation, via Agrobacterium tumefaciens-mediated transformation. The accumulation of the SBP homologue was increased in transgenic plants expressing the heterologous sbp gene, whereas those expressing the antisense construct had reduced levels of the protein. The antisense transgenic plants developed symptoms characteristic of an inhibition of sucrose translocation and displayed a reduction in plant growth and development. In contrast, overexpression of the protein accelerated plant growth and the onset of flowering induction. The overall developmental performance of the transgenic plants was correlated with their photosynthetic rate under normal conditions. While photosynthesis in the antisense lines was decreased, in the sense lines photosynthetic rates were increased. Furthermore, both antisense repression and overexpression of the SBP homologue in transgenic lines altered carbohydrate partitioning in mature leaves. Taken together, these results indicate that S-64 protein is functionally analogous to SBP, representing an important component of the sucrose translocation pathway in plants.
We have obtained Nicotiana tabacum transgenic cell lines expressing a sucrose binding protein (sb... more We have obtained Nicotiana tabacum transgenic cell lines expressing a sucrose binding protein (sbp) homologue gene from soybean (Glycine max L.), designated s-64, either in the sense or antisense orientation. Sense cell lines over-accumulated the S-64 protein, whereas the antisense cell lines had reduced levels of the endogenous homologue protein.
The sucrose binding protein (SBP) from soybean has been implicated as an important component of t... more The sucrose binding protein (SBP) from soybean has been implicated as an important component of the sucrose uptake system. Two SBP genomic clones, gsS641.1 and gsS641.2, which correspond to allelic forms of the GmSBP2/S64 gene, have been isolated and characterized. As a member of the seed storage protein superfamily, it has been shown that the SBP gene structure is similar to vicilin genes with intron/ exon boundaries at conserved positions. Fluorescence in situ hybridization (FISH) suggested that the soybean SBP gene family is represented by at least two non-allelic genes corresponding to the previously isolated GmSBP1 and GmSBP2/S64 cDNAs. These two cDNAs share extensive sequence similarity but are located at different loci in the soybean genome. To investigate transcriptional activation of the GmSBP2 gene, 2 kb 5¢-¯anking sequences of gsS641.1 and gsS641.2 were fused to the b-glucuronidase (GUS) reporter gene and to the green¯uorescent protein (GFP) reporter gene and independently introduced into Nicotiana tabacum by Agrobacterium tumefaciens-mediated transformation. The SBP2 promoter directed expression of both GUS and GFP reporter genes with high speci®city to the phloem of leaves, stems and roots. Thus, the overall pattern of SBP±GUS or SBP±GFP expression is consistent with the involvement of SBP in sucrose translocation-dependent physiological processes.
... Luis Antônio Serrão Contim I ; Carlos Roberto de Carvalho II ; Franciele Alline Martins II ; ... more ... Luis Antônio Serrão Contim I ; Carlos Roberto de Carvalho II ; Franciele Alline Martins II ; Danival Vieira de Freitas III. ... The authors thank Ms. Reginaldo AF Buzelli (Purdue University, USA) and Dr. Charles R. Clement (INPA, Brazil) for helpful discussions and critical reading of ...
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2006
The Glycine max sucrose binding protein (GmSBP2) promoter directs phloem-specific expression of r... more The Glycine max sucrose binding protein (GmSBP2) promoter directs phloem-specific expression of reporter genes in transgenic tobacco. Here, we identified cis-regulatory domains (CRD) that contribute with positive and negative regulation for the tissue-specific pattern of the GmSPB2 promoter. Negative regulatory elements in the distal CRD-A (-2000 to -700) sequences suppressed expression from the GmSBP2 promoter in tissues other than seed tissues and vascular tissues of vegetative organs. Deletion of this region relieved repression resulting in a constitutive promoter highly active in all tissues analyzed. Further deletions from the strong constitutive -700GmSBP2 promoter delimited several intercalating enhancer-like and repressing domains that function in a context-dependent manner. Histochemical examination revealed that the CRD-C (-445 to -367) harbors both negative and positive elements. This region abolished promoter expression in roots and in all tissues of stems except for the inner phloem. In contrast, it restores root meristem expression when fused to the -132pSBP2-GUS construct, which contains root meristem expression-repressing determinants mapped to the 44-bp CRD-G (-136 to -92). Thus, the GmSBP2 promoter is functionally organized into a proximal region with the combinatorial modular configuration of plant promoters and a distal domain, which restricts gene expression to the vascular tissues in vegetative organs.
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