Papers by Kofi Edirisah Aidoo
Food additives and contaminants, Jun 1, 2004
Forty-nine samples of raw cow's milk and 20 sampl... more Forty-nine samples of raw cow's milk and 20 samples of fresh white soft cheese were collected directly from 20 local dairy factories in the north-west of Libya and analysed for the presence of aflatoxin M1 (AFM1). The samples were analysed using a high-performance liquid chromatography technique for toxin detection and quantification. Thirty-five of the 49 milk samples (71.4%) showed AFM1
Journal of Hospital Infection, May 1, 1988
Three of the most commonly used delivery systems in enteral feeding were evaluated for potential ... more Three of the most commonly used delivery systems in enteral feeding were evaluated for potential routes of contamination during assembly and delivery of feeds. Assembly of systems wearing sterile gloves gave no contamination in the feeds but all systems were contaminated when assembled either with bare unprotected hands or with hands experimentally contaminated with bacterial cells. Delivery of contamination-free feed was only possible with the use of sterile gloves.
Clinical Nutrition, Jun 1, 1990
The techniques of opening and decanting ready-to-use enteral feeds packaged in bottles (crown-cap... more The techniques of opening and decanting ready-to-use enteral feeds packaged in bottles (crown-cap and screw-cap), cans and tetrapaks were evaluated as potential routes for the contamination of these feeds. It was found that the outsides of the feed containers, bottle openers, scissors and the experimenters' hands all acted as sources of contamination during the transfer of feeds to the nutrient container. The main source of contamination appeared to be the experimenters' hands with counts up to 10(2) cfu ml(-1) being recorded for feeds that had been decanted from screw-cap bottles, cans and tetrapaks by experimenters with either unprotected bare hands or hands experimentally contaminated with K. aerogenes. Levels of contamination and the number of samples contaminated after opening and decanting were consistently higher for cans and tetrapaks than for crown-cap or screw-cap bottles. Disinfection of feed containers followed by the use of sterile gloves and/or disinfected openers yielded bacteria-free feed from all the types of feed container studied.
International Journal of Environmental Health Research, Sep 1, 1995
... The potential problems of air-borne contamination of intravenous infusions and enterai tube f... more ... The potential problems of air-borne contamination of intravenous infusions and enterai tube feeds have been highlighted by Todd et al. (1990) and Aidoo and Anderton (1990). Thus, Goodley et al. ... 1984, McCray et al. 1986, Johnston et al. ...
Journal of Human Nutrition and Dietetics, Jun 1, 1988
Page 1. Journal of Human Nutrition and Dietetics (19881, 1, 197-203 The effect of handling proced... more Page 1. Journal of Human Nutrition and Dietetics (19881, 1, 197-203 The effect of handling procedures on bacterial contamination of enteral feeds-importance of design of enteral feeding sets Kofi E. Aidoo and Annette Anderton ...
Fems Microbiology Letters, 2005
Strains of Bacillus cereus can produce a heat-stable toxin (cereulide). In this study, 101 Bacill... more Strains of Bacillus cereus can produce a heat-stable toxin (cereulide). In this study, 101 Bacillus strains representing 7 Bacillus species were tested for production of heat-stable toxins. Strains of B. megaterium, B. firmus and B. simplex were found to produce novel heat-stable toxins, which showed varying levels of toxicity. B. cereus strains (18 out of 54) were positive for toxin production. Thirteen were of serovar H1, and it was of interest that some were of clinical origin. Two were of serovars 17B and 20, which are not usually implicated in the emetic syndrome. Partial purification of the novel B. megaterium, B. simplex and B. firmus toxins showed they had similar physical characteristics to the B. cereus emetic toxin, cereulide.
International Biodeterioration & Biodegradation, 1993
ABSTRACT On a world-wide basis, post-harvest losses of durable crops are estimated at 10%, but in... more ABSTRACT On a world-wide basis, post-harvest losses of durable crops are estimated at 10%, but in Africa, Asia and Latin America, losses of 20% are frequently encountered. Attention is now being focused on improving farm-level post-harvest systems in many developing countries for all types of crops, namely staple foods, export crops, secondary food products and animal feeds. The humid tropical climate promotes microbial proliferation on food crops and feeds, often resulting in formation of mycotoxins. Improvements in the traditional post-harvest crop-handling and storage techniques would minimize or eliminate losses caused by biodeteriorative agents. The post-harvest storage of some tropical crops and traditional methods of food preservation are described.
World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences, 2015
Biotechnology Techniques
A technique for the detection of aflatoxins in pistachio and cashew nuts using immunoaffinity col... more A technique for the detection of aflatoxins in pistachio and cashew nuts using immunoaffinity column clean-up with HPLC and fluorescent detection is presented. Recoveries were in the range of 79–99% for pistachio samples artificially contaminated with 10g total aflatoxins kg–1 of food sample. For cashew samples recoveries ranged from 80–106%. This method is proposed as an accurate technique for aflatoxin detection in the range of g aflatoxins kg–1 nuts.
Journal of Food Science and Nutrition
Aflatoxins, are one of over 200 known mycotoxins produced by filamentous fungi. They are toxic, c... more Aflatoxins, are one of over 200 known mycotoxins produced by filamentous fungi. They are toxic, carcinogenic, and mutagenic and may be present in many raw and processed food commodities including cereals and milk used as ingre-dients in infant food products. Consumption of these commodities may pose a potential risk to the health of infants. The mycoflora of 84 samples of baby food commercially available in North Africa was determined. The feeds were screened for mycotoxins using thin layer chromatography (TLC) and further analyses were carried for the total aflatoxins by high performance liquid chromatography (HPLC) using a Kobra cell to derivatise the aflatoxins (B 1 , G 1 , B 2 and G 2). Twenty-four fungal isolates were recovered from the samples consisting of 5 Aspergillus. spp, 13 Penicillum. spp, 5 Mucor. spp and an unidentified species Of these, 45.8% of the isolates were found to be mycotoxigenic however only 2.4% of the samples contained aflatoxins (19 to 70 µg·kg –1) and t...
Mycotoxin Research, 2000
A total of 27 maize-based products destined for human consumption were collected from retail outl... more A total of 27 maize-based products destined for human consumption were collected from retail outlets within the city of Glasgow in the UK and were analysed for the presence of aflatoxins using immunoaftinity column chromatography with fluorescence detection and for fumonisins by competitive ELISA. Aflatoxins were detected at a trace level below 4 in eight (30%) of the 27 samples tested, no sample contained aflatoxins at a high level although one sample of sweetcorn did contain aflatoxins at a level of 5-10 Fumonisins were detected in eight (30%) of the samples at levels from 1 to 8mgkg(-1) and a further eight samples contained fumonisin at a level below 1 mgkg(-1) but above the detectable level. The highest concentration of fumonisins was found in a sample of fine corn meal at 8-12mgkg(-1).
Advances in Applied Microbiology Volume 28, 1982
Publisher Summary The solid-state fermentation refers to any fermentation that takes place on sol... more Publisher Summary The solid-state fermentation refers to any fermentation that takes place on solid or semisolid substrate or that occurs in a nutritionally inert solid support, which provides some advantage to the microorganism with respect to access to nutrients. In a solid-state fermentation, several factors must be taken into consideration to achieve high efficiency of the overall process. Some of the general characteristics include: the solid substrate must be in a form to allow free circulation of air, usually the only other medium component required is water, and control of temperature is sometimes critical. Moisture content of the solid substrate is an important factor during microbial growth in a solid-state fermentation process. There are many advantages of the solid-state fermentation processes over the conventional stirred tank system on both laboratory and large scale. Some of the advantages for work involving fungi are—the medium is relatively simple, the conditions under which the fungus grows are more like the conditions in its natural habitat, and the desired product may be readily extracted from the vessel by addition of solvent directly.
Pharmaceutical Biology, 2006
... In contrast, MAM700, QBL, and MMD contain 8 to 11 plant ingredients but were not cytotoxic. .... more ... In contrast, MAM700, QBL, and MMD contain 8 to 11 plant ingredients but were not cytotoxic. ... Previous section. Aziz NH, Youssef YA, El-Fouly MZ, Moussa LA (1998): Contamination of some common medicinal plant samples and spices by fungi and their mycotoxins. ...
Mycopathologia, 2008
Twenty commercial mixed herbal drugs were examined for mycological profile. Aspergillus species w... more Twenty commercial mixed herbal drugs were examined for mycological profile. Aspergillus species were the predominant fungi found in the drugs. Other fungi harboured in the drugs with less frequency were Paecilomyces species, Eurotium species, Monascus species, Acremonium species, Penicillium species, Cladosporium species, Scopulariopsis species, Phialophora species and Fonseceae species. Fungal count was between 1.0 log(10) CFU and 2.4 log(10) CFU per gram of sample. When the drugs were incubated in 85% humidity at 25 degrees C, fungal colonies grew on only two of the drugs. The mixed herbal drugs were extracted with water and the extracts were used to grow Aspergillus parasiticus. All extracts reduced aflatoxin B(1) and aflatoxin G(1) production by 62-97%. All but two of the extracts reduced aflatoxin B(2) and aflatoxin G(2) production by 39-95%. It can be concluded that the commercial powdered mixed herbal drugs contained low number of endogenous fungi, and these drugs are inhibitory to the growth of its endogenous fungi and aflatoxins production by aflatoxigenic fungi.
Journal of the Science of Food and Agriculture, 2002
Sixteen varieties of date fruit (Phoenix dactylifera) at three stages of maturation (Kimri, Rutab... more Sixteen varieties of date fruit (Phoenix dactylifera) at three stages of maturation (Kimri, Rutab and Tamr) were examined for the presence of fungi and analysed for aflatoxins B1, B2, G1 and G2 and sterigmatocystin. Single samples of each variety were used in the study. Samples as received were initially examined for mycoflora and toxin levels and then stored at 98% relative humidity and 30 °C for 14 days to investigate the effects of possible adverse storage conditions on mycoflora and, in particular, aflatoxin formation. All samples showed an absence of aflatoxins and their precusor, sterigmatocystin, after adverse storage for 14 days, although aflatoxin‐producing Aspergillus flavus isolates were identified in 10 varieties at the first stage of maturation (Kimri). High fungal counts were associated with the Rutab stage and low counts with the Tamr stage. The counts of A flavus ranged from 5.00 to 8.16 log10(cfu g−1) under simulated storage conditions, and three varieties contained...
Journal of the Science of Food and Agriculture, 2004
Journal of Human Nutrition and Dietetics, 1988
Page 1. Journal of Human Nutrition and Dietetics (19881, 1, 197-203 The effect of handling proced... more Page 1. Journal of Human Nutrition and Dietetics (19881, 1, 197-203 The effect of handling procedures on bacterial contamination of enteral feeds-importance of design of enteral feeding sets Kofi E. Aidoo and Annette Anderton ...
Journal of Hospital Infection, 1991
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Papers by Kofi Edirisah Aidoo