R.J. Knight1, S.J. Patel2, K. Achkar3, G. Land4, S. Dilioglou5, J. Hamilton6, P. Barretto7, A.O. ... more R.J. Knight1, S.J. Patel2, K. Achkar3, G. Land4, S. Dilioglou5, J. Hamilton6, P. Barretto7, A.O. Gaber1 1Department Of Surgery, The Methodist Hospital, Houston/TX/ UNITED STATES OF AMERICA, 2, The Methodist Hospital, Houston/TX/UNITED STATES OF AMERICA, 3Internal Medicine, The Methodist Hospital, Houston/UNITED STATES OF AMERICA, 4Pathology, The Methodist Hospital, Houston/UNITED STATES OF AMERICA, 5Pathology, The Methodist Hospital, Houston/TX/UNITED STATES OF AMERICA, 6Transplant Center, The Methodist Hospital, Houston/TX/UNITED STATES OF AMERICA, 7Transplant Center, The Methodist Hospital, Houston/UNITED STATES OF AMERICA
Background: It is reported that the genetic alteration of p53 is associated with neovascularizati... more Background: It is reported that the genetic alteration of p53 is associated with neovascularization during the progression of glioma to its more malignant form, glioblastoma. BAIl which was identified as a p53-target gene, is specifically expressed in the brain, however is not expressed in gioblastoma cell lines, suggesting that BAIl plays a significant role in angiogenesis inhibitor as a mediator of p53. We already demonstrated that BAIl transfected glioblastoma failed to form tumor neovascularization. Purpose: In an attempt to gain insight into this effect, we checked the expression of this gene in pancreatic cancer cell lines. Furthermore, we transfected BAIl gene to a pancreatic cancer cell line using an adenoviral vector and monitored the in vitro and in vivo growth in severe combined immune-deficient (SCID) mice. Materials and Methods: RT-PCR analysis revealed that BAIl gene was not expressed in any of the eight pancreatic cancer cell lines. After p53 transfection using an adenoviral vector, BAIl expression was induced in pancreatic cancer cell line (Pane1) as well as colon cancer cell lines (T84 and HT29). We transfected BAIl gene into a pancreatic cancer cell line (PancIBAIl) using an adenoviral vector and monitored both in vitro growth and in vivo growth using scm mice. LacZ
Pulmonary alveolar proteinosis (PAP) has been associated with the immunosuppressant sirolimus in ... more Pulmonary alveolar proteinosis (PAP) has been associated with the immunosuppressant sirolimus in transplant patients. PAP is a progressive lung disease characterized by the accumulation of surfactant-like material in the lungs leading to decreased pulmonary function with shortness of breath and cough as common symptoms. We report a rare case of sirolimus-associated PAP in a kidney transplant recipient with a history of end-stage renal disease secondary to haemolytic uraemic syndrome (HUS) and review of the literature. Discontinuation of sirolimus and initiation of tacrolimus led to resolution of PAP without recurrence of HUS.
Heart views : the official journal of the Gulf Heart Association, 2013
Contrast-induced nephropathy (CIN) is a serious complication of angiographic procedures resulting... more Contrast-induced nephropathy (CIN) is a serious complication of angiographic procedures resulting from the administration of contrast media (CM). It is the third most common cause of hospital acquired acute renal injury and represents about 12% of the cases. CIN is defined as an elevation of serum creatinine (Scr) of more than 25% or ≥0.5 mg/dl (44 μmol/l) from baseline within 48 h. More sensitive markers of renal injury are desired, therefore, several biomarkers of tubular injury are under evaluation. Multiple risk factors may contribute to the development of CIN; these factors are divided into patient- and procedure-related factors. Treatment of CIN is mainly supportive, consisting mainly of careful fluid and electrolyte management, although dialysis may be required in some cases. The available treatment option makes prevention the corner stone of management. This article will review the recent evidence concerning CIN incidence, diagnosis, and prevention strategies as well as its ...
R.J. Knight1, S.J. Patel2, K. Achkar3, G. Land4, S. Dilioglou5, J. Hamilton6, P. Barretto7, A.O. ... more R.J. Knight1, S.J. Patel2, K. Achkar3, G. Land4, S. Dilioglou5, J. Hamilton6, P. Barretto7, A.O. Gaber1 1Department Of Surgery, The Methodist Hospital, Houston/TX/ UNITED STATES OF AMERICA, 2, The Methodist Hospital, Houston/TX/UNITED STATES OF AMERICA, 3Internal Medicine, The Methodist Hospital, Houston/UNITED STATES OF AMERICA, 4Pathology, The Methodist Hospital, Houston/UNITED STATES OF AMERICA, 5Pathology, The Methodist Hospital, Houston/TX/UNITED STATES OF AMERICA, 6Transplant Center, The Methodist Hospital, Houston/TX/UNITED STATES OF AMERICA, 7Transplant Center, The Methodist Hospital, Houston/UNITED STATES OF AMERICA
Background: It is reported that the genetic alteration of p53 is associated with neovascularizati... more Background: It is reported that the genetic alteration of p53 is associated with neovascularization during the progression of glioma to its more malignant form, glioblastoma. BAIl which was identified as a p53-target gene, is specifically expressed in the brain, however is not expressed in gioblastoma cell lines, suggesting that BAIl plays a significant role in angiogenesis inhibitor as a mediator of p53. We already demonstrated that BAIl transfected glioblastoma failed to form tumor neovascularization. Purpose: In an attempt to gain insight into this effect, we checked the expression of this gene in pancreatic cancer cell lines. Furthermore, we transfected BAIl gene to a pancreatic cancer cell line using an adenoviral vector and monitored the in vitro and in vivo growth in severe combined immune-deficient (SCID) mice. Materials and Methods: RT-PCR analysis revealed that BAIl gene was not expressed in any of the eight pancreatic cancer cell lines. After p53 transfection using an adenoviral vector, BAIl expression was induced in pancreatic cancer cell line (Pane1) as well as colon cancer cell lines (T84 and HT29). We transfected BAIl gene into a pancreatic cancer cell line (PancIBAIl) using an adenoviral vector and monitored both in vitro growth and in vivo growth using scm mice. LacZ
Pulmonary alveolar proteinosis (PAP) has been associated with the immunosuppressant sirolimus in ... more Pulmonary alveolar proteinosis (PAP) has been associated with the immunosuppressant sirolimus in transplant patients. PAP is a progressive lung disease characterized by the accumulation of surfactant-like material in the lungs leading to decreased pulmonary function with shortness of breath and cough as common symptoms. We report a rare case of sirolimus-associated PAP in a kidney transplant recipient with a history of end-stage renal disease secondary to haemolytic uraemic syndrome (HUS) and review of the literature. Discontinuation of sirolimus and initiation of tacrolimus led to resolution of PAP without recurrence of HUS.
Heart views : the official journal of the Gulf Heart Association, 2013
Contrast-induced nephropathy (CIN) is a serious complication of angiographic procedures resulting... more Contrast-induced nephropathy (CIN) is a serious complication of angiographic procedures resulting from the administration of contrast media (CM). It is the third most common cause of hospital acquired acute renal injury and represents about 12% of the cases. CIN is defined as an elevation of serum creatinine (Scr) of more than 25% or ≥0.5 mg/dl (44 μmol/l) from baseline within 48 h. More sensitive markers of renal injury are desired, therefore, several biomarkers of tubular injury are under evaluation. Multiple risk factors may contribute to the development of CIN; these factors are divided into patient- and procedure-related factors. Treatment of CIN is mainly supportive, consisting mainly of careful fluid and electrolyte management, although dialysis may be required in some cases. The available treatment option makes prevention the corner stone of management. This article will review the recent evidence concerning CIN incidence, diagnosis, and prevention strategies as well as its ...
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Papers by K. Achkar