Sob uma vertente metodológica jurídico-crítico-descritiva, explana-se sob a matriz teórica de Pau... more Sob uma vertente metodológica jurídico-crítico-descritiva, explana-se sob a matriz teórica de Paulo Freire, a “Pedagogia da Autonomia”, para mostrar a relação do professor-aluno no contexto educação. Logo após, aduz dados sobre a violência contra a população trans e sua marginalização. Em seguida, mostra-se como que o projeto artístico pedagógico “Transvest” cumpre essa proposta de educação transformadora dentro da realidade da população trans com suas bases e objetivos específicos. Conclui-se sobre a viabilidade da teoria apresentada e da necessidade de expansão de projetos/ONGs/cursinho como o descrito no texto.
The identification of laboratory markers which predict the outcome of COVID-19 patients is a grea... more The identification of laboratory markers which predict the outcome of COVID-19 patients is a great concern. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) has been used to confirm the clinical diagnosis. The aim of this study is to evaluate laboratory parameters of COVID-19 patients as well as to evaluate the RT-PCR crossing point (Cp) value and correlate blood test abnormalities and the Cp value with patients survival. Two hundred thirty patients with positive RT-PCR of SARS-CoV-2 were included in the study. Molecular diagnosis of SARS-CoV-2 was performed by RT-PCR (LightMix, TibMolbiol, Germany). Clinical information, biochemical parameters and Cp values were collected in an anonymized database and variables were analyzed with SPSS v25.0 (IBM Corporation, Armonk, NY, USA). No-survivors were significantly older (>65 years old) than survivors (p=0.007). A higher prevalence of cardiovascular comorbidities in patients who died than in those who survived was foun...
T he new coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 (severe acute respiratory syndr... more T he new coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) emerged in China in October-November 2019 (ref. 1) and by the end of March 2020 it was present in most countries of the world. The World Health Organization (WHO) declared the new disease as a pandemic on 11 March 2020. Spain suffered a severe epidemic, with the first case reported on 29 January 2020 (ref. 2), and an accumulated number of 249,659 cases by 1 July 2020, including 28,363 fatalities 3. Furthermore, a nationwide seroprevalence study showed that only one in ten cases of infection by SARS-CoV-2 were diagnosed and reported in that period 4 , suggesting that the total number of infections has been vastly underestimated. Spain ordered a series of nonpharmaceutical intervention measures, including a general lockdown on
Implications of gray zone results for routine hepatitis C virus screening with the ARCHITECT HCV-... more Implications of gray zone results for routine hepatitis C virus screening with the ARCHITECT HCV-Ag assay Abbreviated running headline: HCV-Ag gray zone results importance.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Jan 6, 2018
To analyse the antimicrobial phenotype, carbapenem mechanisms, integrons, virulence factors and m... more To analyse the antimicrobial phenotype, carbapenem mechanisms, integrons, virulence factors and molecular typing of 164 Pseudomonas aeruginosa isolates recovered from lower tract respiratory samples in a Spanish hospital (1 year) as well as the patients' clinical data. Susceptibility testing to 12 antipseudomonal agents was determined by microdilution and metallo-beta-lactamase (MBL) phenotype by double disc method. The oprD gene was studied by PCR, sequencing and comparison with P. aeruginosa PAO1 sequence. Detection and characterisation of MBLs, class 1, 2 and 3 integrons, and virulence genes were studied by PCR and sequencing. The prevalence of carbapenem-resistant P. aeruginosa (CRPA) was 26.8%. MBL phenotype was detected in 52.3% CRPA, and all of them were disseminated throughout the intensive care unit. Most of the MBL-carrying patients presented respiratory disease, mechanical ventilation, tracheostomy, bacteraemia, ≥ 30 hospitalisation days and previous treatment with ca...
is a major opportunistic human pathogen, responsible for nosocomial infections and infections in ... more is a major opportunistic human pathogen, responsible for nosocomial infections and infections in patients with impaired immune systems. Little data exist about the faecal colonisation by isolates in healthy humans. The occurrence, antimicrobial resistance phenotype, virulence genotype, and genetic lineages of from faecal samples of children from two different Spanish regions were characterised. Seventy-two were isolated from 1,443 faecal samples. Low antimicrobial resistance levels were detected: ceftazidime (8%), cefepime (7%), aztreonam (7%), gentamicin (3%), ciprofloxacin (1%), and imipenem (1%); susceptibility to meropenem, amikacin, tobramycin, levofloxacin, and colistin. Four multidrug-resistant strains were found. Important differences were detected between both geographical regions. Forty-one sequence types were detected among the 48 tested strains. Virulence and quorum sensing genes were analysed and 13 virulotypes were detected, being 26 -positive strains. Alteration in pr...
Enfermedades Infecciosas y Microbiología Clínica, 2021
OBJECTIVE Serratia marcescens is a Gram-negative bacterium that is found in hospital environments... more OBJECTIVE Serratia marcescens is a Gram-negative bacterium that is found in hospital environments and commonly associated with outbreaks in neonatal units. One S. marcescens isolate was detected from a bloodstream culture from a neonate in our hospital that was followed by an outbreak. The aim of this study was to describe the molecular epidemiology of a S. marcescens outbreak in the neonatal unit. METHODS In order to investigate the outbreak, weekly surveillance rectal swabs were submitted for culture from all patients admitted in this unit from August to September 2018. Environmental samples were obtained from potential sources in September 2018. Typing of isolates was performed by pulsed field gel electrophoresis (PFGE). In addition, we studied the in vitro activity of chlorhexidine against S. marcescens. RESULTS During this period, 146 infants were hospitalised in our neonatal unit, of which 16 patients had a S. marcescens-positive sample. A total of 36 environmental surveillance samples were collected, and one sample from a stethoscope from an incubator of a colonized baby was positive for S. marcescens. All the 18 isolates, including the isolate from the stethoscope, belonged to a single PFGE cluster. We found that very low concentrations of chlorhexidine, even with application times close to 0 achieved significant reductions in the amount of S. marcescens. CONCLUSION A unique clone of S. marcescens caused this outbreak, including isolates from patients and from one stethoscope. The outbreak was controlled with the early implementation of specific control measures.
Sob uma vertente metodológica jurídico-crítico-descritiva, explana-se sob a matriz teórica de Pau... more Sob uma vertente metodológica jurídico-crítico-descritiva, explana-se sob a matriz teórica de Paulo Freire, a “Pedagogia da Autonomia”, para mostrar a relação do professor-aluno no contexto educação. Logo após, aduz dados sobre a violência contra a população trans e sua marginalização. Em seguida, mostra-se como que o projeto artístico pedagógico “Transvest” cumpre essa proposta de educação transformadora dentro da realidade da população trans com suas bases e objetivos específicos. Conclui-se sobre a viabilidade da teoria apresentada e da necessidade de expansão de projetos/ONGs/cursinho como o descrito no texto.
The identification of laboratory markers which predict the outcome of COVID-19 patients is a grea... more The identification of laboratory markers which predict the outcome of COVID-19 patients is a great concern. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) has been used to confirm the clinical diagnosis. The aim of this study is to evaluate laboratory parameters of COVID-19 patients as well as to evaluate the RT-PCR crossing point (Cp) value and correlate blood test abnormalities and the Cp value with patients survival. Two hundred thirty patients with positive RT-PCR of SARS-CoV-2 were included in the study. Molecular diagnosis of SARS-CoV-2 was performed by RT-PCR (LightMix, TibMolbiol, Germany). Clinical information, biochemical parameters and Cp values were collected in an anonymized database and variables were analyzed with SPSS v25.0 (IBM Corporation, Armonk, NY, USA). No-survivors were significantly older (>65 years old) than survivors (p=0.007). A higher prevalence of cardiovascular comorbidities in patients who died than in those who survived was foun...
T he new coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 (severe acute respiratory syndr... more T he new coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) emerged in China in October-November 2019 (ref. 1) and by the end of March 2020 it was present in most countries of the world. The World Health Organization (WHO) declared the new disease as a pandemic on 11 March 2020. Spain suffered a severe epidemic, with the first case reported on 29 January 2020 (ref. 2), and an accumulated number of 249,659 cases by 1 July 2020, including 28,363 fatalities 3. Furthermore, a nationwide seroprevalence study showed that only one in ten cases of infection by SARS-CoV-2 were diagnosed and reported in that period 4 , suggesting that the total number of infections has been vastly underestimated. Spain ordered a series of nonpharmaceutical intervention measures, including a general lockdown on
Implications of gray zone results for routine hepatitis C virus screening with the ARCHITECT HCV-... more Implications of gray zone results for routine hepatitis C virus screening with the ARCHITECT HCV-Ag assay Abbreviated running headline: HCV-Ag gray zone results importance.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Jan 6, 2018
To analyse the antimicrobial phenotype, carbapenem mechanisms, integrons, virulence factors and m... more To analyse the antimicrobial phenotype, carbapenem mechanisms, integrons, virulence factors and molecular typing of 164 Pseudomonas aeruginosa isolates recovered from lower tract respiratory samples in a Spanish hospital (1 year) as well as the patients' clinical data. Susceptibility testing to 12 antipseudomonal agents was determined by microdilution and metallo-beta-lactamase (MBL) phenotype by double disc method. The oprD gene was studied by PCR, sequencing and comparison with P. aeruginosa PAO1 sequence. Detection and characterisation of MBLs, class 1, 2 and 3 integrons, and virulence genes were studied by PCR and sequencing. The prevalence of carbapenem-resistant P. aeruginosa (CRPA) was 26.8%. MBL phenotype was detected in 52.3% CRPA, and all of them were disseminated throughout the intensive care unit. Most of the MBL-carrying patients presented respiratory disease, mechanical ventilation, tracheostomy, bacteraemia, ≥ 30 hospitalisation days and previous treatment with ca...
is a major opportunistic human pathogen, responsible for nosocomial infections and infections in ... more is a major opportunistic human pathogen, responsible for nosocomial infections and infections in patients with impaired immune systems. Little data exist about the faecal colonisation by isolates in healthy humans. The occurrence, antimicrobial resistance phenotype, virulence genotype, and genetic lineages of from faecal samples of children from two different Spanish regions were characterised. Seventy-two were isolated from 1,443 faecal samples. Low antimicrobial resistance levels were detected: ceftazidime (8%), cefepime (7%), aztreonam (7%), gentamicin (3%), ciprofloxacin (1%), and imipenem (1%); susceptibility to meropenem, amikacin, tobramycin, levofloxacin, and colistin. Four multidrug-resistant strains were found. Important differences were detected between both geographical regions. Forty-one sequence types were detected among the 48 tested strains. Virulence and quorum sensing genes were analysed and 13 virulotypes were detected, being 26 -positive strains. Alteration in pr...
Enfermedades Infecciosas y Microbiología Clínica, 2021
OBJECTIVE Serratia marcescens is a Gram-negative bacterium that is found in hospital environments... more OBJECTIVE Serratia marcescens is a Gram-negative bacterium that is found in hospital environments and commonly associated with outbreaks in neonatal units. One S. marcescens isolate was detected from a bloodstream culture from a neonate in our hospital that was followed by an outbreak. The aim of this study was to describe the molecular epidemiology of a S. marcescens outbreak in the neonatal unit. METHODS In order to investigate the outbreak, weekly surveillance rectal swabs were submitted for culture from all patients admitted in this unit from August to September 2018. Environmental samples were obtained from potential sources in September 2018. Typing of isolates was performed by pulsed field gel electrophoresis (PFGE). In addition, we studied the in vitro activity of chlorhexidine against S. marcescens. RESULTS During this period, 146 infants were hospitalised in our neonatal unit, of which 16 patients had a S. marcescens-positive sample. A total of 36 environmental surveillance samples were collected, and one sample from a stethoscope from an incubator of a colonized baby was positive for S. marcescens. All the 18 isolates, including the isolate from the stethoscope, belonged to a single PFGE cluster. We found that very low concentrations of chlorhexidine, even with application times close to 0 achieved significant reductions in the amount of S. marcescens. CONCLUSION A unique clone of S. marcescens caused this outbreak, including isolates from patients and from one stethoscope. The outbreak was controlled with the early implementation of specific control measures.
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Papers by Jessica Bueno