Izhar khan

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Hi Everyone I'm Izhar I belong to Pakistan, Doing MSc In sociology From International Islamic University Islamabad Pakistan. Interest in Sociology, Sociology of ethnicity. Sociology of Health, Sociology of Gender Studies.
Phone: +923115000668

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Papers by Izhar khan

Identification and further characterization of Streptococcus uberis and Streptococcus parauberis isolated from bovine milk samples

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Quantitative Campylobacter spp., antibiotic resistance genes, and veterinary antibiotics in surface and ground water following manure application: Influence of tile drainage control

The Science of the total environment, Jan 8, 2015

This work investigated chlortetracycline, tylosin, and tetracycline (plus transformation products... more This work investigated chlortetracycline, tylosin, and tetracycline (plus transformation products), and DNA-based quantitative Campylobacter spp. and Campylobacter tetracycline antibiotic resistant genes (tet(O)) in tile drainage, groundwater, and soil before and following a liquid swine manure (LSM) application on clay loam plots under controlled (CD) and free (FD) tile drainage. Chlortetracycline/tetracycline was strongly bound to manure solids while tylosin dominated in the liquid portion of manure. The chlortetracycline transformation product isochlortetracycline was the most persistent analyte in water. Rhodamine WT (RWT) tracer was mixed with manure and monitored in tile and groundwater. RWT and veterinary antibiotic (VA) concentrations were strongly correlated in water which supported the use of RWT as a surrogate tracer. While CD reduced tile discharge and eliminated application-induced VA movement (via tile) to surface water, total VA mass loading to surface water was not a...

Arcobacter lanthieri sp. nov., isolated from pig and dairy cattle manure

by Zaky Adam and Izhar khan

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2015

A study was undertaken to determine the prevalence and diversity of Arcobacter species in pig and... more A study was undertaken to determine the prevalence and diversity of Arcobacter species in pig and dairy cattle manure that led to the identification of the strains AF1440T, AF1430 and AF1581, respectively. Initially identified as Arcobacter butzleri based on colony morphology and initial PCR-confirmation tests, analyses of 16S rRNA gene sequences of these strains belonged to the genus Arcobacter and were different from all known Arcobacter species. The isolates formed a distinct group within the genus Arcobacter based on their 16S rRNA, gyrB, rpoB, cpn60, gyrA and atpA gene sequences and fatty acid profiles. The unique species status was further supported by physiological properties and DNA-DNA hybridization that allowed phenotypic and genotypic differentiation of strains from other Arcobacter spp. The isolates were found to be oxidase, catalase and esterase positive, and urease negative; they grew well at 30 °C under microaerophilic condition and produced NO2 and Acetoin, respectively. Based on the common origin and various physiological properties it is proposed that the isolates are classified as members of a novel species with the name Arcobacter lanthieri sp. nov., with the strains AF1440, AF1430, AF1581; type strain AF1440T (=LMG 28516T; CCUG 66485T), AF1430 (=LMG 28515; CCUG 66486) and AF1581 (=LMG 28517; CCUG 66487), respectively.

The distribution of Salmonella enterica serovars and subtypes in surface water from five agricultural regions across Canada

Water research, Jan 3, 2015

Serovar prevalence of the zoonotic pathogen, Salmonella enterica, was compared among 1624 surface... more Serovar prevalence of the zoonotic pathogen, Salmonella enterica, was compared among 1624 surface water samples collected previously from five different Canadian agricultural watersheds over multiple years. Phagetyping, pulsed field gel electrophoresis (PFGE), and antimicrobial resistance subtyping assays were performed on serovars Enteritidis, Typhimurium, and Heidelberg. Serovars and subtypes from surface water were compared with those from animal feces, human sewage, and serovars reported to cause salmonellosis in Canadians. Sixty-five different serovars were identified in surface water; only 32% of these were isolated from multiple watersheds. Eleven of the 13 serovars most commonly reported to cause salmonellosis in Canadians were identified in surface water; isolates of these serovars constituted >40% of the total isolates. Common phagetypes and PFGE subtypes of serovars associated with illness in humans such as S. Enteritidis and S. Typhimurium were also isolated from surf...

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Development of a single-tube, cell lysis-based, genus-specific PCR method for rapid identification of mycobacteria: optimization of cell lysis, PCR primers and conditions, and restriction pattern analysis

Journal of clinical microbiology, 2004

A single-tube PCR method was developed for efficient identification of nontuberculous mycobacteri... more A single-tube PCR method was developed for efficient identification of nontuberculous mycobacteria (NTM) and their environmental isolates in about 3 h without conventional DNA isolation. The following three steps were optimized or developed: (i). a simple, 6-min direct cell lysis protocol as a PCR prestep for generation of DNA-template, (ii). an improved Mycobacterium-specific PCR amplification protocol with a broader species specificity using newly designed primers targeting a 228-bp region of the 65-kDa heat shock protein (hsp) gene and optimal PCR amplification conditions, and (iii). a genus-specific restriction analysis of the PCR product for conclusive identification of the unknown NTM isolates.

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Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods

Journal of veterinary science, 2003

In the present study 130 S. uberis strains and one S. parauberis strain isolated from bovine milk... more In the present study 130 S. uberis strains and one S. parauberis strain isolated from bovine milk samples of 58 different farms of various locations in Hesse, Germany, as well as two reference strains of each species were comparatively investigated for cultural, biochemical, serological and molecular properties. All S. uberis strains produced the enzyme beta-D-glucuronidase, while the S. parauberis strains were negative. The S. uberis and S. parauberis 16S rRNA genes were amplified by polymerase chain reaction and subsequently digested with the restriction enzymes RsaI and AvaII yielding species-specific restriction patterns. Both species were additionally identified by amplifying species-specific parts of the genes encoding the 16S rRNA, the 23S rRNA and the 16S-23S rDNA intergenic spacer region, respectively. The CAMP factor gene cfu, a potential virulence factor of S. uberis, was amplified, corresponding to a phenotypically positive CAMP-reaction, using cfu-specific oligonucleoti...

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Comparative assessment of next-generation sequencing, denaturing gradient gel electrophoresis, clonal restriction fragment length polymorphism and cloning-sequencing as methods for characterizing commercial microbial consortia

Journal of Microbiological Methods, 2015

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Progress Report on Investigation of Waterborne Pathogen Occurrence in Source Water of Lake Ontario Drinking Water Treatment Plants near the Credit River (2007-2008)

Paper: IDENTIFICATION OF STREPTOCOCCUS DYSGALACTIAE STRAINS OF LANCEFIELD’S GROUP C, G AND L BY POLYMERASE CHAIN REACTION

Mycobacteria in Metalworking Fluids: Development of DNA-based Methods for Exposure Assessment

Enhanced recovery and real-time PCR based quantification of mycobacteria from metalworking fluids

Seroprevalence of brucellosis in animals in district kohat NWFP and comparison of two serological tests

Identification of Streptococcus dysgalactiae strains of Lancefield's group C, G and L by polymerase chain reaction

Streptococcus dysgalactiae serogroup C, G and L strains were investigated by polymerase chain rea... more Streptococcus dysgalactiae serogroup C, G and L strains were investigated by polymerase chain reaction (PCR) using oligonucleotide primers designed according to species-specific parts of the 16S-23S rDNA intergenic spacer region. The oligonucleotide primers with specificity for the 16S-23S rDNA intergenic spacer region allowed a correct identification of all S. dysgalactiae serogroups C, G and L strains investigated. No cross-reactivities could be observed with any of the control strains indicating the usefulness of PCR-technology to identify the serologically heterogeneous species S. dysgalactiae.

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Susceptibility of Mycobacterium immunogenum and Pseudomonas fluorescens to Formaldehyde and Non-Formaldehyde Biocides in Semi-Synthetic Metalworking Fluids

International Journal of Molecular Sciences, 2011

Mycobacterium immunogenum, a newly identified member of the Mycobacterium chelonae_M. abscessus c... more Mycobacterium immunogenum, a newly identified member of the Mycobacterium chelonae_M. abscessus complex is considered a potential etiological agent for hypersensitivity pneumonitis (HP) in machine workers exposed to contaminated metalworking fluid (MWF). This study investigated the biocidal efficacy of the frequently applied commercial formaldehyde-releasing (HCHO) biocides Grotan and Bioban CS 1135 and non-HCHO type biocides Kathon 886 MW (isothiazolone) and Preventol CMK 40 (phenolic) toward this emerging mycobacterial species (M. immunogenum) in HP-linked MWFs, alone and in presence of a representative of the Gram-negative bacterial contaminants, Pseudomonas fluorescens, using two semi-synthetic MWF matrices (designated Fluid A and Fluid B). Relative biocide susceptibility analysis indicated M immunogenum to be comparatively more resistant (2-1600 fold) than P. fluorescens to the tested biocides under the varied test conditions. In terms of minimum inhibitory concentration, Kathon was the most effective biocide against M. immunogenum. Fluid

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Investigation of the prevalence of thermophilic Campylobacter species at Lake Simcoe recreational beaches

Inland Waters, 2013

Thermophilic Campylobacter species have been implicated in human gastrointestinal infections and ... more Thermophilic Campylobacter species have been implicated in human gastrointestinal infections and can occur in agricultural run off, sewage discharges, and the feces of domestic and wild animals including birds. A 2-year study was designed to investigate the occurrence of the primary thermophilic Campylobacter species (C. jejuni, coli, and lari) associated with human disease at 5 recreational beaches on Lake Simcoe, Ontario, Canada. A biweekly sampling program involved collecting water samples across 3 depth zones (sand pore water and ankle-and chest-depth waters). To identify the potential sources of contamination, samples were also collected from 4 neighboring rivers corresponding to selected beaches, a few fresh seagull and Canada geese fecal droppings on beaches, and a stormwater outfall. Water and fecal samples were processed for Campylobacter spp. isolation and detection using a minimum probable number culture enrichment protocol. Thermophilic Campylobacter spp. generally occurred infrequently and at low concentrations (≤30 cells L −1 ) at all sampling locations; they were detected in 12% of water samples from beaches (n = 289) compared to 14% from rivers (n = 100). C. jejuni and C. lari were the species most commonly detected. Nine isolates identified as unknown Campylobacter spp. were further sequenced and shown to be more closely related to Arcobacter spp. At beaches, thermophilic Campylobacter spp. were generally detected more often in sand pore water than in ankle-or chest-depth water. The study suggests that sand, rivers, and bird droppings could be potential sources of Campylobacter spp. contamination at Lake Simcoe recreational beaches.

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Draft Genome Sequences of Three Arcobacter Strains of Pig and Dairy Cattle Manure Origin

Genome Announcements, 2014

The genus Arcobacter has been associated with human illness and fecal contamination by humans and... more The genus Arcobacter has been associated with human illness and fecal contamination by humans and animals. Here, we announce the draft genome sequences of three strains of Arcobacter species cultured from pig and dairy cattle manure tanks. This information will assist in the characterization of features related to host specificities and identify potential pathogenic health risks to humans and animals.

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Draft Genome Sequence of Pantoea ananatis Strain LMG 2665T, a Bacterial Pathogen of Pineapple Fruitlets

Genome Announcements, 2014

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Draft Genome Sequence of Arcobacter cibarius Strain LMG21996T, Isolated from Broiler Carcasses

Genome Announcements, 2014

The draft genome sequence of Arcobacter cibarius strain LMG21996(T), isolated from chicken carcas... more

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Draft Genome Sequences of Two Arcobacter Strains Isolated from Human Feces

Genome Announcements, 2014

Arcobacter species are members of the family Campylobacteraceae and are considered emerging enter... more Arcobacter species are members of the family Campylobacteraceae and are considered emerging enteropathogens and potential zoonotic agents. Here, we report the draft genome sequences of two Arcobacter strains isolated from human feces in an effort to provide further genetic resources for understanding the pathogenic dynamics and diversity of this important genus.

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A National Investigation of the Prevalence and Diversity of Thermophilic Campylobacter Species in Agricultural Watersheds in Canada

Water Research, 2014

The occurrence and diversity of thermophilic Campylobacter species (C. jejuni, coli, and lari) we... more The occurrence and diversity of thermophilic Campylobacter species (C. jejuni, coli, and lari) were studied in water samples from four river basins located across Canada. These basins located in Quebec (Bras d&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Henri), Alberta (Oldman), Ontario (South Nation), and British Columbia (Sumas) represented some of the most intensive farming areas in Canada for hog, beef cattle, dairy cattle, and poultry, respectively. This study analyzed 769 water samples collected from 23 monitoring sites with agricultural influence, and four reference sites with limited or no agricultural influence. Water samples were collected bi-weekly over two years and analyzed for Campylobacter using a semi-quantitative minimum probable number (MPN) enrichment protocol. Putative isolates were confirmed by genus- and species-specific multiplex polymerase chain reaction (PCR) assays. A total of 377 (49%) water samples were positive for campylobacters with 355 samples having a cell density ranging from 4 to 4000 MPN L(-1). Campylobacters were more common at agricultural than reference sites in each river basin, although this difference was not significant in the Oldman and South Nation (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05). Campylobacter was significantly more common in the Bras d&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Henri and Sumas (63%) compared to the South Nation (45%) and Oldman (33%) River basins (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05). C. jejuni, C. coli and C. lari were detected in each river basin, and these species occurred in 45% (n = 168), 34% (n = 128) and 19% (n = 73), of all Campylobacter positive samples, respectively. The remaining Campylobacter positive water samples without these three species (n = 67; 18%) were identified as other Campylobacter species. C. jejuni was the predominant species occurring in the Sumas, Oldman and South Nation River basins. However, in the Bras d&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;Henri River basin with intensive hog production, C. coli was the predominant species. This study found campylobacters to be common in some agricultural systems with intensive livestock farming activities, and different river basins could have strikingly different profiles of either C. jejuni or C. coli as the predominant waterborne thermophilic Campylobacter species.

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Identification and further characterization of Streptococcus uberis and Streptococcus parauberis isolated from bovine milk samples

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Quantitative Campylobacter spp., antibiotic resistance genes, and veterinary antibiotics in surface and ground water following manure application: Influence of tile drainage control

The Science of the total environment, Jan 8, 2015