Papers by Gregory Gregoriadis
Vaccine, 2000
The recombinant Schistosoma mansoni 62 kDa myosin fragment, rIrV-5, is highly protective in exper... more The recombinant Schistosoma mansoni 62 kDa myosin fragment, rIrV-5, is highly protective in experimental animals, however, vaccination of mice and rats with the recombinant Schistosoma japonicum homologue, rSj62, did not induce significant resistance against S. japonicum infection. To explore alternative ways of presenting this antigen, we further constructed a plasmid (VRSj62) which encodes Sj62 using the VR1020 vector and tested it in vaccination experiments. Four immunisations with 10 microg VRSj62 DNA alone were sufficient to induce high and progressively increasing levels of IgG antibodies against rSj62 with increasing numbers of injections in CBA/Ca mice (IgG titre > or =1:25000), and three injections with 50 microg VRSj62 DNA alone induced significant IgG responses in C57Bl/6 mice (IgG titre, 1:1600). However, vaccination with plasmid DNA entrapped in cationic liposomes or together with pUC19 DNA as a source of CpG motifs, both of which have been reported to enhance immune responses, did not enhance specific antibody production. In spite of the stimulation of specific antibodies against rSj62 with the naked DNA construct no resistance to challenge was demonstrated.
Clinical Immunology Newsletter, 1981
Journal of Liposome Research, 1995
Journal of Liposome Research, 1999
Drug Develop Ind Pharm, 1987
Methods in Enzymology, 2005
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1997
Journal of Biological Chemistry, Nov 10, 1970
When desialylated ceruloplasmin (ASCPN), labeled with 64Cu and with 3H in its galactose residues,... more When desialylated ceruloplasmin (ASCPN), labeled with 64Cu and with 3H in its galactose residues, is injected intravenously into a rat, more than half of the radioactivity can be recovered within minutes in ASCPN precipitated immunochemically from liver homogenates.
The Journal of Biological Chemistry, Mar 10, 1971
Evidence is presented to indicate a generalized role for the terminal sialic acid residues of cir... more Evidence is presented to indicate a generalized role for the terminal sialic acid residues of circulating glycoproteins. Upon injection into rats, all of the desialylated plasma proteins tested, with the exception of transfer& were promptly removed from the circulation and were recovered from the liver. The materials examined included orosomucoid, fetuin, ceruloplasmin, haptoglobin, arz-macroglobulin, thyroglobulin, lactoferrin, and the two gonadotropic hormones, human chorionic gonadotropin and follicle-stimulating hormone.
Life Sciences, 1977
... ATTEMPTS TO IMPROVE LOCALIZATION IN TUMOUR TISSUES Gregory Gregoriadis, Diane E. Neerunjun an... more ... ATTEMPTS TO IMPROVE LOCALIZATION IN TUMOUR TISSUES Gregory Gregoriadis, Diane E. Neerunjun and Ruth Hunt Clinical Research Centre, Watford Road, Harrow, Middx., II.K. (Received in final form June 27 ... L. KRUPP, AV CHOBANIAN and PI BRECHER, Biochem. ...
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 2016
Previously, we showed that CD206-targeted liposomal delivery of co-encapsulated immunodominant my... more Previously, we showed that CD206-targeted liposomal delivery of co-encapsulated immunodominant myelin basic protein (MBP) sequences MBP46-62, MBP124-139 and MBP147-170 (Xemys) suppressed experimental autoimmune encephalomyelitis in dark Agouti rats. The objective of this study was to assess the safety of Xemys in the treatment of patients with relapsing-remitting multiple sclerosis (MS) and secondary progressive MS, who failed to achieve a sustained response to first-line disease-modifying therapies. In this phase I, open-label, dose-escalating, proof-of-concept study, 20 patients with relapsing-remitting or secondary progressive MS received weekly subcutaneously injections with ascending doses of Xemys up to a total dose of 2.675 mg. Clinical examinations, including Expanded Disability Status Scale score, magnetic resonance imaging results, and serum cytokine concentrations, were assessed before the first injection and for up to 17 weeks after the final injection. Xemys was safe an...
Methods in Enzymology, Feb 1, 1976
J Pharmaceut Biomed Anal, 1997
Haloperidol (Hal), a highly hydrophobic drug, was complexed with two β-cyclodextrin (β-CD) deriva... more Haloperidol (Hal), a highly hydrophobic drug, was complexed with two β-cyclodextrin (β-CD) derivatives. Hal solubility was increased 20-fold in the presence of a 10-fold excess of methyl β-CD (Meβ-CD) and 12-fold in the presence of a 10-fold excess of 2-hydroxypropyl β-CD (HPβ-CD). The stoichiometries and stability constants of Hal–Meβ-CD (1:1 and 2345 M−1 at 27°C) and Hal–HPβ-CD (1:1 and 2112 M−1 at 27°C) complexes were calculated by the continuous variation and phase solubility methods respectively. Differential scanning calorimetry and 1H-NMR were used to confirm the formation of inclusion complexes. Moreover, the enthalpy and entropy of the complexation process were calculated for both complexes in order to obtain such information as the main `driving force' and whether or not complex formation is thermodynamically favoured. This was achieved by monitoring the isothermic solubility lines at various temperatures.
Sub Cellular Biochemistry, Feb 1, 1989
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Papers by Gregory Gregoriadis