This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
The consortium of thermotolerant petroleum-oxidizing bacteria containing strains Gordonia sp. 1D ... more The consortium of thermotolerant petroleum-oxidizing bacteria containing strains Gordonia sp. 1D VKM Ac-2720 D, Rhodococcus sp. Par7 VKM Ac-2722 D, and R. pyridinivorans L5A-BSU VKM Ac-2721 for destruction of oil and oil products in hot climates was developed for the first time. The consortium was effective in soils and liquid media at temperature as high as 50°C, at salinity up to 7%, and soil moisture of about 10%. The efficiency of petroleum destruction for 21 days was 70 and 59% at 24 and 45°C, respectively. The consortium of thermotolerant petroleum-destructing strains could be used as basis for the biopreparation for remediation of petroleum-contaminated soils and waters in hot climates.
Polycyclic aromatic hydrocarbons (PAHs) are chemically recalcitrant carcinogenic and mutagenic co... more Polycyclic aromatic hydrocarbons (PAHs) are chemically recalcitrant carcinogenic and mutagenic compounds with primarily anthropogenic origin. The investigation of the effects of emissions from energy enterprises on soil microbiomes is of a high priority for modern soil science. In this study, metagenomic profiling of technogenic contaminated soils was carried out based on bioinformatic analysis of shotgun metagenome data with PAH-degrading genes identification. The use of prokaryotic consortia has been often used as one of the bio-remediation approaches to degrade PAHs with different molecular weight. Since the process of PAH degradation predominantly includes non-culturable or yet-to-be cultured species, metagenomic approaches are highly recommended for studying the composition and metabolic abilities of microbial communities. In this study, whole metagenome shotgun sequencing of DNA from two soils with varying PAH levels was performed. In the control site, the total content of 12 ...
Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and... more Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and the Antarctic; 13 of 86 strains proved to be thermotolerant. These bacteria utilized crude oil at 45-50°C; their growth optimum (35-37°С) and range (20-53°С) differ from those of mesophilic bacteria. Thermotolerant strains were identified as representatives of the genera Rhodococcus and Gordonia. It was shown that their ability to degrade petroleum products does not differ at 24 and 45°С. The strains Rhodococcus sp. Par7 and Gordonia sp. 1D utilized 14 and 20% of the oil, respectively, in 14 days at 45°С. All of the isolated thermotolerant bacteria grew in a medium containing 3% NaCl; the medium for the strains Gordonia amicalis 1B and Gordonia sp. 1D contained up to 10% NaCl. The bacteria G. amicalis and Rhodococcus erythropolis were able to utilize crude oil and individual hydrocarbons at higher (up to 50°С) temperatures.
Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s... more Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s putida BS3701 (pBS1141, pBS1142), Pseudomonas putida BS3745 (pBS216), and Burkholderia sp. BS3702 (pBS1143) were studied in model soil systems. The differences in accumulation and uptake rate of phenanthrene intermediates between the strains under study have been shown. Accumulation of 1-hydroxy-2-naphthoic acid in soil in the course of
Introduction to Enhanced Oil Recovery (EOR) Processes and Bioremediation of Oil-Contaminated Sites, 2012
In Russia, this ecological problem is of a special significance because of the scale of the oil s... more In Russia, this ecological problem is of a special significance because of the scale of the oil spills when soil excavation and restoration ex-situ are impossible. Bioremediation, which consists on the microorganisms' ability of utilizing and transforming oil hydrocarbons, plays the main role during in-situ remediation. Bioremediation provides an economically
The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was ... more The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was studied in laboratory soil microcosms. Conjugation transfer of catabolic plasmids was demonstrated in naphthalene-contaminated soil. Both indigenous microorganisms and an introduced laboratory strain BS394 (pNF142::TnMod-OTc) served as donors of these plasmids. The indigenous bacterial degraders of naphthalene isolated from soil were identified as Pseudomonas putida and Pseudomonas fluorescens.
Journal of Chemical Technology & Biotechnology, 2006
The kinetic parameters of the naphthalene degradation process performed by indigenous and introdu... more The kinetic parameters of the naphthalene degradation process performed by indigenous and introduced microorganisms were studied in soil model systems. The introduction of active naphthalenedegrading strains Pseudomonas putida BS3701 and G7 in soil model systems accelerated the naphthalene destruction process by a factor of three to four. Moreover, the addition of salicylate (0.1 mg g −1 dry soil) to the systems containing the introduced microbial strains again doubled the rate of the naphthalene degradation process. To provide a quantitative assessment of the naphthalene biodegradation process, a mathematical model describing the bacterial growth, the consumption of the naphthalene, the production and subsequent consumption of naphthalene cleavage products, and the consumption of organic soil substances in soil model systems was developed. An approach for assessment of the degradation efficiency of low solubility polycyclic aromatic hydrocarbon provided by bacteria of genera Pseudomonas and Burkholderia in soil was suggested. The approach will enable comparison and selection of the most active degraders, which have the potential for application in biotechnologies for cleaning of soils contaminated by polycyclic aromatic hydrocarbons.
A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442... more A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442(pNF142:: TnMod-OTc), has been constructed. The presence of the gfp gene (which codes for green fluorescent protein) and the kanamycin and rifampicin resistance genes in the chromosome of this strain allows the strain’s fate in model soil systems to be monitored, whereas a minitransposon, inserted into naphthalene biodegradation plasmid pNF142 and containing
The problem of eliminating petroleum pollution and its consequences is currently very relevant fo... more The problem of eliminating petroleum pollution and its consequences is currently very relevant for Kazakhstan, which is among the ten largest oil-producing countries. The specifics of natural conditions—the sharply continental arid climate—necessitate the development and application of adequate technologies for the restoration of oil-contaminated territories and the Caspian seashore. The key factors (temperature, moisture, alkalinity, salinity, low mineral and organic matter content) affect the self-purification processes and microbiological status of oil-contaminated soils of Kazakhstan. The assessment of taxonomic diversity and characteristics of oil-degrading microorganisms isolated from samples of soils and reservoirs contaminated with hydrocarbons are given. The review of biopreparations and biotechnologies developed and used in Kazakhstan for cleaning environments from oil pollution is made, and their effectiveness is shown. The analysis of the current state of research in the...
The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its mi... more The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its microbial degradation were studied for the first time. The tagged naphthalene-degrading strains bearing labeled biodegradation plasmids were used for the monitoring of horizontal plasmid transfer in open soil. The population kinetics of microorganisms, the survival rate and competitiveness of introduced strains, and the transfer of biodegradation plasmids
Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s... more Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s putida BS3701 (pBS1141, pBS1142), Pseudomonas putida BS3745 (pBS216), and Burkholderia sp. BS3702 (pBS1143) were studied in model soil systems. The differences in accumulation and uptake rate of phenanthrene intermediates between the strains under study have been shown. Accumulation of 1-hydroxy-2-naphthoic acid in soil in the course of
Introduction to Enhanced Oil Recovery (EOR) Processes and Bioremediation of Oil-Contaminated Sites, 2012
... Andrey Filonov1, Anastasia Ovchinnikova1, Anna Vetrova1, Irina Puntus1, Irina Nechaeva2, Kiri... more ... Andrey Filonov1, Anastasia Ovchinnikova1, Anna Vetrova1, Irina Puntus1, Irina Nechaeva2, Kirill Petrikov2, Elena Vlasova2, Lenar Akhmetov1 ... with hydrocarbons Pilot testing of the biopreparation was performed in the territory of JSC “Tulskaya Toplivno-Energeticheskaya ...
A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442... more A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442(pNF142:: TnMod-OTc), has been constructed. The presence of the gfp gene (which codes for green fluorescent protein) and the kanamycin and rifampicin resistance genes in the chromosome of this strain allows the strain’s fate in model soil systems to be monitored, whereas a minitransposon, inserted into naphthalene biodegradation plasmid pNF142 and containing
The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its mi... more The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its microbial degradation were studied for the first time. The tagged naphthalene-degrading strains bearing labeled biodegradation plasmids were used for the monitoring of horizontal plasmid transfer in open soil. The population kinetics of microorganisms, the survival rate and competitiveness of introduced strains, and the transfer of biodegradation plasmids
The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was ... more The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was studied in laboratory soil microcosms. Conjugation transfer of catabolic plasmids was demonstrated in naphthalene-contaminated soil. Both indigenous microorganisms and an introduced laboratory strain BS394 (pNF142::TnMod-OTc) served as donors of these plasmids. The indigenous bacterial degraders of naphthalene isolated from soil were identified as Pseudomonas putida and Pseudomonas fluorescens.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
The consortium of thermotolerant petroleum-oxidizing bacteria containing strains Gordonia sp. 1D ... more The consortium of thermotolerant petroleum-oxidizing bacteria containing strains Gordonia sp. 1D VKM Ac-2720 D, Rhodococcus sp. Par7 VKM Ac-2722 D, and R. pyridinivorans L5A-BSU VKM Ac-2721 for destruction of oil and oil products in hot climates was developed for the first time. The consortium was effective in soils and liquid media at temperature as high as 50°C, at salinity up to 7%, and soil moisture of about 10%. The efficiency of petroleum destruction for 21 days was 70 and 59% at 24 and 45°C, respectively. The consortium of thermotolerant petroleum-destructing strains could be used as basis for the biopreparation for remediation of petroleum-contaminated soils and waters in hot climates.
Polycyclic aromatic hydrocarbons (PAHs) are chemically recalcitrant carcinogenic and mutagenic co... more Polycyclic aromatic hydrocarbons (PAHs) are chemically recalcitrant carcinogenic and mutagenic compounds with primarily anthropogenic origin. The investigation of the effects of emissions from energy enterprises on soil microbiomes is of a high priority for modern soil science. In this study, metagenomic profiling of technogenic contaminated soils was carried out based on bioinformatic analysis of shotgun metagenome data with PAH-degrading genes identification. The use of prokaryotic consortia has been often used as one of the bio-remediation approaches to degrade PAHs with different molecular weight. Since the process of PAH degradation predominantly includes non-culturable or yet-to-be cultured species, metagenomic approaches are highly recommended for studying the composition and metabolic abilities of microbial communities. In this study, whole metagenome shotgun sequencing of DNA from two soils with varying PAH levels was performed. In the control site, the total content of 12 ...
Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and... more Oil-degrading bacteria were isolated from soil and water samples taken in Russia, Kazakhstan, and the Antarctic; 13 of 86 strains proved to be thermotolerant. These bacteria utilized crude oil at 45-50°C; their growth optimum (35-37°С) and range (20-53°С) differ from those of mesophilic bacteria. Thermotolerant strains were identified as representatives of the genera Rhodococcus and Gordonia. It was shown that their ability to degrade petroleum products does not differ at 24 and 45°С. The strains Rhodococcus sp. Par7 and Gordonia sp. 1D utilized 14 and 20% of the oil, respectively, in 14 days at 45°С. All of the isolated thermotolerant bacteria grew in a medium containing 3% NaCl; the medium for the strains Gordonia amicalis 1B and Gordonia sp. 1D contained up to 10% NaCl. The bacteria G. amicalis and Rhodococcus erythropolis were able to utilize crude oil and individual hydrocarbons at higher (up to 50°С) temperatures.
Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s... more Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s putida BS3701 (pBS1141, pBS1142), Pseudomonas putida BS3745 (pBS216), and Burkholderia sp. BS3702 (pBS1143) were studied in model soil systems. The differences in accumulation and uptake rate of phenanthrene intermediates between the strains under study have been shown. Accumulation of 1-hydroxy-2-naphthoic acid in soil in the course of
Introduction to Enhanced Oil Recovery (EOR) Processes and Bioremediation of Oil-Contaminated Sites, 2012
In Russia, this ecological problem is of a special significance because of the scale of the oil s... more In Russia, this ecological problem is of a special significance because of the scale of the oil spills when soil excavation and restoration ex-situ are impossible. Bioremediation, which consists on the microorganisms' ability of utilizing and transforming oil hydrocarbons, plays the main role during in-situ remediation. Bioremediation provides an economically
The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was ... more The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was studied in laboratory soil microcosms. Conjugation transfer of catabolic plasmids was demonstrated in naphthalene-contaminated soil. Both indigenous microorganisms and an introduced laboratory strain BS394 (pNF142::TnMod-OTc) served as donors of these plasmids. The indigenous bacterial degraders of naphthalene isolated from soil were identified as Pseudomonas putida and Pseudomonas fluorescens.
Journal of Chemical Technology & Biotechnology, 2006
The kinetic parameters of the naphthalene degradation process performed by indigenous and introdu... more The kinetic parameters of the naphthalene degradation process performed by indigenous and introduced microorganisms were studied in soil model systems. The introduction of active naphthalenedegrading strains Pseudomonas putida BS3701 and G7 in soil model systems accelerated the naphthalene destruction process by a factor of three to four. Moreover, the addition of salicylate (0.1 mg g −1 dry soil) to the systems containing the introduced microbial strains again doubled the rate of the naphthalene degradation process. To provide a quantitative assessment of the naphthalene biodegradation process, a mathematical model describing the bacterial growth, the consumption of the naphthalene, the production and subsequent consumption of naphthalene cleavage products, and the consumption of organic soil substances in soil model systems was developed. An approach for assessment of the degradation efficiency of low solubility polycyclic aromatic hydrocarbon provided by bacteria of genera Pseudomonas and Burkholderia in soil was suggested. The approach will enable comparison and selection of the most active degraders, which have the potential for application in biotechnologies for cleaning of soils contaminated by polycyclic aromatic hydrocarbons.
A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442... more A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442(pNF142:: TnMod-OTc), has been constructed. The presence of the gfp gene (which codes for green fluorescent protein) and the kanamycin and rifampicin resistance genes in the chromosome of this strain allows the strain’s fate in model soil systems to be monitored, whereas a minitransposon, inserted into naphthalene biodegradation plasmid pNF142 and containing
The problem of eliminating petroleum pollution and its consequences is currently very relevant fo... more The problem of eliminating petroleum pollution and its consequences is currently very relevant for Kazakhstan, which is among the ten largest oil-producing countries. The specifics of natural conditions—the sharply continental arid climate—necessitate the development and application of adequate technologies for the restoration of oil-contaminated territories and the Caspian seashore. The key factors (temperature, moisture, alkalinity, salinity, low mineral and organic matter content) affect the self-purification processes and microbiological status of oil-contaminated soils of Kazakhstan. The assessment of taxonomic diversity and characteristics of oil-degrading microorganisms isolated from samples of soils and reservoirs contaminated with hydrocarbons are given. The review of biopreparations and biotechnologies developed and used in Kazakhstan for cleaning environments from oil pollution is made, and their effectiveness is shown. The analysis of the current state of research in the...
The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its mi... more The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its microbial degradation were studied for the first time. The tagged naphthalene-degrading strains bearing labeled biodegradation plasmids were used for the monitoring of horizontal plasmid transfer in open soil. The population kinetics of microorganisms, the survival rate and competitiveness of introduced strains, and the transfer of biodegradation plasmids
Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s... more Degradation of phenanthrene by strains Pseudomona, Moscow, KMK, 2004simova, I.A. and Chernov, I.s putida BS3701 (pBS1141, pBS1142), Pseudomonas putida BS3745 (pBS216), and Burkholderia sp. BS3702 (pBS1143) were studied in model soil systems. The differences in accumulation and uptake rate of phenanthrene intermediates between the strains under study have been shown. Accumulation of 1-hydroxy-2-naphthoic acid in soil in the course of
Introduction to Enhanced Oil Recovery (EOR) Processes and Bioremediation of Oil-Contaminated Sites, 2012
... Andrey Filonov1, Anastasia Ovchinnikova1, Anna Vetrova1, Irina Puntus1, Irina Nechaeva2, Kiri... more ... Andrey Filonov1, Anastasia Ovchinnikova1, Anna Vetrova1, Irina Puntus1, Irina Nechaeva2, Kirill Petrikov2, Elena Vlasova2, Lenar Akhmetov1 ... with hydrocarbons Pilot testing of the biopreparation was performed in the territory of JSC “Tulskaya Toplivno-Energeticheskaya ...
A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442... more A genetically tagged, plasmid-containing, naphthalene-degrading strain, Pseudomonas putida KT2442(pNF142:: TnMod-OTc), has been constructed. The presence of the gfp gene (which codes for green fluorescent protein) and the kanamycin and rifampicin resistance genes in the chromosome of this strain allows the strain’s fate in model soil systems to be monitored, whereas a minitransposon, inserted into naphthalene biodegradation plasmid pNF142 and containing
The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its mi... more The horizontal transfer of naphthalene biodegradation plasmids and the parallel process of its microbial degradation were studied for the first time. The tagged naphthalene-degrading strains bearing labeled biodegradation plasmids were used for the monitoring of horizontal plasmid transfer in open soil. The population kinetics of microorganisms, the survival rate and competitiveness of introduced strains, and the transfer of biodegradation plasmids
The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was ... more The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was studied in laboratory soil microcosms. Conjugation transfer of catabolic plasmids was demonstrated in naphthalene-contaminated soil. Both indigenous microorganisms and an introduced laboratory strain BS394 (pNF142::TnMod-OTc) served as donors of these plasmids. The indigenous bacterial degraders of naphthalene isolated from soil were identified as Pseudomonas putida and Pseudomonas fluorescens.
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