Papers by FERNANDO VIZCAINO
BMC Bioinformatics, 2009
The computational prediction of mycobacterial proteins' subcellular localization is of key import... more The computational prediction of mycobacterial proteins' subcellular localization is of key importance for proteome annotation and for the identification of new drug targets and vaccine candidates. Several subcellular localization classifiers have been developed over the past few years, which have comprised both general localization and feature-based classifiers. Here, we have validated the ability of different bioinformatics approaches, through the use of SignalP 2.0, TatP 1.0, LipoP 1.0, Phobius, PA-SUB 2.5, PSORTb v.2.0.4 and Gpos-PLoc, to predict secreted bacterial proteins. These computational tools were compared in terms of sensitivity, specificity and Matthew's correlation coefficient (MCC) using a set of mycobacterial proteins having less than 40% identity, none of which are included in the training data sets of the validated tools and whose subcellular localization have been experimentally confirmed. These proteins belong to the TBpred training data set, a computational tool specifically designed to predict mycobacterial proteins.
Ps-political Science & Politics, 2006
Se describe el material disponible de un dinosaurio estegosaurio recogido en la localidad de Moça... more Se describe el material disponible de un dinosaurio estegosaurio recogido en la localidad de Moçafaneira (Torres Vedras, Portugal). El individuo consiste en un esqueleto incompleto hallado en niveles pertenecientes al Grupo Lourinhã (Jurásico Superior de Portugal). La presencia de vértebras dorsales con centros masivos más anchos que largos y con marcadas concavidades laterales y, la existencia de un proceso prepúbico muy ancho, permiten asignar el ejemplar en estudio al estegosáurido primitivo Dacentrurus armatus. Además, se realiza una síntesis del material de Dacentrurus descrito en Europa.
Veterinary Microbiology, 2010
Diabetes Care, 2010
We used confirmatory factor analysis to test whether a single factor might explain the clustering... more We used confirmatory factor analysis to test whether a single factor might explain the clustering of the metabolic syndrome (MS) components in children. We studied 1,020 children aged 10-13 years from 20 schools in Cuenca, Spain. The single-factor model included: waist circumference (WC), fasting insulin, triglyceride to HDL cholesterol ratio (Triglyl/HDL-C), and mean arterial pressure (MAP). The standardized scores of the four variables in the model were used to develop a continuous MS index. Factor loadings were 0.67 for WC, 0.68 for fasting insulin, 0.57 for Triglyl/HDL-C, and 0.37 for MAP. The single-factor model also showed a good fit to the data. As compared with Adult Treatment Panel III criteria, the MS index showed strong validity in the diagnosis of MS (area under the receiver operating characteristic curve = 0.98, 95% CI 0.96-0.99). A single underlying factor has acceptable validity to represent MS in children.
Acta Paediatrica, 2007
Aim: To examine the association of body mass index (BMI), triceps skinfold thickness (TST) and pe... more Aim: To examine the association of body mass index (BMI), triceps skinfold thickness (TST) and percentage body fat (%BF) from bioelectrical impedance analysis (BIA) with blood lipids, systolic blood pressure (SBP) and diastolic blood pressure (DBP) in children.Methods: Cross-sectional study was conducted on 1280 schoolchildren aged 8–11 years from the Cuenca province (Spain). Data collection was conducted under standardized conditions, taking several measurements of each variable to enhance accuracy. Analyses were performed using age-adjusted correlation coefficients, and multiple linear regression adjusted for age, BMI, TST and %BF.Results: Correlations between %BF and apolipoprotein (apo) B, total cholesterol, low-density lipoprotein cholesterol (LDL-c), total cholesterol/HDL-c ratio and DBP were higher than those for BMI and TST. In contrast, the correlations between BMI, and apo A-I and SBP were higher than those for %BF and TST. The results were similar across the sexes. The correlations between each of the three measures of body fatness, and blood lipids and blood pressure were highest in children with greatest BMI and %BF. When analyses were adjusted for the three body fatness measures, %BF showed stronger associations than did BMI or TST with blood lipids and blood pressure, with the exception of apo A-I and SBP, which were more closely associated with BMI.Conclusion: %BF from BIA is more strongly associated than either BMI or TST with most of the blood lipid fractions in schoolchildren aged 8–11 years.
Journal of Pediatrics, 2010
To assess the impact of a 2-year recreational physical activity program in 1044 fourth- and fifth... more To assess the impact of a 2-year recreational physical activity program in 1044 fourth- and fifth-grade primary schoolchildren from the Province of Cuenca, Spain. Cluster-randomized controlled trial with 10 intervention and 10 control schools. The program consisted of 3 90-minute sessions of physical activity per week,during 28 weeks every year.Changes in endpoints between baseline (September 2004) and the end of follow-up (June 2006) were compared between the control and intervention group by using mixed regression models, with adjustment for the baseline endpoint value, age, and the school. Compared with control subjects, intervention girls reduced the frequency of overweight (odds ratio, 0.55; 95% CI, 0.39-0.78; P<.001). However, intervention was associated with an increase in the percentage of body fat in boys (0.97%; 95% CI, 0.14-1.81; P=.02). Girls in the intervention group had lower total cholesterol level (-6.86 mg/dL; 95% CI, -9.70--4.01; P<.001) and apolipoprotein B level (-3.61 mg/dL; 95% CI, -6.27--0.95; P=.008) than control subjects. Results were similar in boys. In 2 years, the physical activity program lowered the frequency of overweight in girls and reduced total cholesterol and apolipoprotein B in both girls and boys.
Research in Veterinary Science, 2011
Cetacean morbillivirus (CeMV) is considered the most pathogenic virus in cetaceans. Three strains... more Cetacean morbillivirus (CeMV) is considered the most pathogenic virus in cetaceans. Three strains have been already described: the dolphin morbillivirus (DMV), the porpoise morbillivirus (PMV) and the tentatively named pilot whale morbillivirus (PWMV).
Medical and Veterinary Entomology, 2010
During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elap... more During the 2003–2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.
Avian Pathology, 2010
Newcastle disease (ND) causes large economic losses in poultry production worldwide. Spain has re... more Newcastle disease (ND) causes large economic losses in poultry production worldwide. Spain has reported two ND epidemics in poultry farms since 1993, the most recent in 2009. The recent increase in the number of ND epidemics reported in Spain and in other European Union (EU) member countries along with the failure to identify the source of the Spanish epidemics caused concern over the vulnerability that Spain has to the disease. Some of the epidemics recently reported in EU member states were associated with legal introduction of live poultry; the large number of susceptible species annually imported by Spain from the EU suggests that legal imports of poultry may impose a risk for the introduction of virulent ND virus (v-NDV) into the country. This article presents the results of the first quantitative assessment of the risk for v-NDV introduction into an ND-free country via legal trade of live poultry. The geographical variation of the risk and the relative contribution of exporting countries and susceptible poultry species to the risk were also estimated. The model here estimated that if prevailing conditions persist, then it would be expected that ND epidemics caused by legal trade of live poultry will occur, on average, once every 196 years in Spain. These results suggest that the risk for ND epidemics in Spain, and probably the sources of recent epidemics reported in the country, were associated with routes of entry other than legal trade of poultry.
Risk Analysis, 2010
Highly pathogenic avian influenza (HPAI) is considered one of the most important diseases of poul... more Highly pathogenic avian influenza (HPAI) is considered one of the most important diseases of poultry. During the last 9 years, HPAI epidemics have been reported in Asia, the Americas, Africa, and in 18 countries of the European Union (EU). For that reason, it is possible that the risk for HPAI virus (HPAIV) introduction into Spain may have recently increased. Because of the EU free-trade policy and because legal trade of live poultry was considered an important route for HPAI spread in certain regions of the world, there are fears that Spain may become HPAIV-infected as a consequence of the legal introduction of live poultry. However, no quantitative assessment of the risk for HPAIV introduction into Spain or into any other EU member state via the trade of poultry has been published in the peer-reviewed literature. This article presents the results of the first quantitative assessment of the risk for HPAIV introduction into a free country via legal trade of live poultry, along with estimates of the geographical variation of the risk and of the relative contribution of exporting countries and susceptible poultry species to the risk. The annual mean risk for HPAI introduction into Spain was estimated to be as low as 1.36 × 10−3, suggesting that under prevailing conditions, introduction of HPAIV into Spain through the trade of live poultry is unlikely to occur. Moreover, these results support the hypothesis that legal trade of live poultry does not impose a significant risk for the spread of HPAI into EU member states.
Mientras sus hermanos estudiaban, se casaban, tenían hijos y dirigían el negocio de la familia, d... more Mientras sus hermanos estudiaban, se casaban, tenían hijos y dirigían el negocio de la familia, desaprovechando su vida junto con el resto de la humanidad anónima en las rutinas diarias de la subsistencia, la procreación y el ocio, él, como un Prometeo redivivo, se esforzaba por echar luz sobre el más oscuro e inaccesible rincón del conocimiento.
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Papers by FERNANDO VIZCAINO