International Journal of Current Microbiology and Applied Sciences, 2020
The most direct benefit is the production of lysozyme, acidity (pH 4.5-5.9) which impart antifung... more The most direct benefit is the production of lysozyme, acidity (pH 4.5-5.9) which impart antifungal and antimicrobial properties, and defensins (HBD-2,HBD-3), antimicrobial peptide marker LL-37, HBD-2,HBD-3,lantibiotics, and other molecules that act to resist co-colonization by pathogens(
Soil saltiness is a noteworthy issue as it results in loss of profitability and development of ag... more Soil saltiness is a noteworthy issue as it results in loss of profitability and development of agrarian harvests and decline in soil health. Microorganisms associated with plants contribute to their growth promotion and salinity tolerance by employing a multitude of macromolecules and pathways. Plant growth promoting rhizobacteria (PGPR) have an immediate impact on improving profitability based on higher crop yield. Some PGPR produce 1-aminocyclopropane-1-carboxylic (ACC) deaminase (EC 4.1.99.4), which controls ethylene production by diverting ACC into α-ketobutyrate and ammonia. ACC deaminase enhances germination rate and growth parameters of root and shoot in different harvests with and without salt stress. Arbuscular mycorrhizal fungi (AMF) show a symbiotic relationship with plants, which helps in efficient uptake of mineral nutrients and water by the plants and also provide protection to the plants against pathogens and various abiotic stresses. The dual inoculation of PGPR and ...
Lipoxygenases by generating pro-inflammatory mediators called leukotriens or anti-inflammatory me... more Lipoxygenases by generating pro-inflammatory mediators called leukotriens or anti-inflammatory mediators called lipoxins are involved in the regulation of several inflammatory responses. They are knows as a group of oxidative enzymes along with a non-heme iron atom in their active sites. We were focused on expression pattern of arachidonic lipoxygenase genes to investigate which of the LOX gene in human plays most versatile role in different and tissues and cell lines so that consensus can be established about this protein family and more research can pay more attention. In this in-silico study we analyzed the different lipoxygenase gene expression patterns in different tissues and cell lines to address issues related to lipoxygenase in several inflammatory disease and cancer. Tissue and cell line datasets of for all lipoxygenase genes were retrieved from atlas and Human protein atlas databases. Further data were analyzed and visualized using R package. ALOX5 and ALOX5AP are highly ...
Agriculture is the best foundation for human livelihoods, and, in this respect, crop production h... more Agriculture is the best foundation for human livelihoods, and, in this respect, crop production has been forced to adopt sustainable farming practices. However, soil salinity severely affects crop growth, the degradation of soil quality, and fertility in many countries of the world. This results in the loss of profitability, the growth of agricultural yields, and the step-by-step decline of the soil nutrient content. Thus, researchers have focused on searching for halotolerant and plant growth-promoting bacteria (PGPB) to increase soil fertility and productivity. The beneficial bacteria are frequently connected with the plant rhizosphere and can alleviate plant growth under salinity stress through direct or indirect mechanisms. In this context, PGPB have attained a unique position. The responses include an increased rate of photosynthesis, high production of antioxidants, osmolyte accumulation, decreased Na+ ions, maintenance of the water balance, a high germination rate, and well-d...
Plant growth promoting bacteria (PGPB) play an important role in agricultural production and soil... more Plant growth promoting bacteria (PGPB) play an important role in agricultural production and soil fertility. Wheat is a highly consumed cereal food crop of the world population and sustainable wheat productivity achieved by the application of bacterium in combination with NPK is promising. In the present study bacterial isolate (PR6) from soil of organic farm was included. The isolate (PR6) was screened for its morphological, biochemical and plant growth promoting characteristics, sequenced by 16S rDNA method and submitted to NCBI for the confirmation of strain identification. Further, the inoculation effect of the bacterial isolate in combination with NPK on growth and yield parameters of wheat var. AAI-W6 were analysed. The isolate (PR6) was identified as Erwinia sp. with NCBI Accession No. KP226572. The organism possessed multiple plant growth promoting (MPGP) traits such as production of ammonia, siderophore, indole-3-acetic acid (IAA), ACC Deaminase (ACCD) and showed phosphate ...
This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in... more This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in lentil (Lens culinaris L.) crop, in the department of microbiology . In this experiment five treatment were taken as Un-inoculated (T1), Rhizobium (T2), PSB + Rhizobium (T3), VAM + Rhizobium (T4) and Rhizobium + PSB + VAM (T5). The experiment was carried out during Rabi 2010 in 2.0 m plot size at pot culture house. The result of this study revealed that the least nitrogen content in grain and straw was found in uninoculated micro plat (T1) whereas maximum nitrogen content was noticed in Rhizobium + PSB + VAM treated field (T5). Lowest phosphorous content in grain and straw was found in uninoculated micro plat (T1) whereas maximum nitrogen content was noticed in Rhizobium + PSB + VAM treated field (T5). Least nitrogen uptake in grain and straw was found in uninoculated micro plat (T1) whereas maximum nitrogen uptake was noticed in Rhizobium + PSB + VAM treated field (T5). The lowest phos...
Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture, 2019
Recent genome sequencing of Trichoderma species has opened the way for their diversity in the ass... more Recent genome sequencing of Trichoderma species has opened the way for their diversity in the assessment and comparative analysis of microsatellites. Here, we compared microsatellites of three taxonomically different Trichoderma species such as T. asperellum, T. citrinoviride, and T. longibrachiatum. Our results highlight abundance and diversity of microsatellite patterns in all the test genomes. The highest relative abundance (283.6) with a relative density (3424.7) of SSRs was identified in all three sequence sets of T. citrinoviride. Among all three sequence sets, selection of these isolates were based on maximum frequency of SSRs which was of trinucleotide repeats (79.8%), whereas the dinucleotide repeat represented <8%. With the help of bioinformatics tools, species-specific diagnostic primers were developed and validated by silica PCR. Out of 31 primer sets, only 18 pairs of primer indicated successful amplification among all the species tested. A total of 34 alleles were d...
International journal of ecology and environmental sciences, 2019
Increased industrialization and urbanization created ecological and toxicological problems from t... more Increased industrialization and urbanization created ecological and toxicological problems from the release of toxic contaminants into the environment. Conventional toxicity bioassays that use animals are time consuming and expensive. The V. fischeri bioassay in which the inhibition of light emission by V. fischeri is considered a rapid, sensitive and simple test for toxicity as compared to other biological methods and assays using algae, protozoa and fish which require 7-10 days. In the present study the suitability of V. fischeri bioassay for the evaluation of toxicity of municipal and industrial wastewater in Allahabad (India) was conducted. Twenty five municipal wastewater streams in city of Allahabad and industrial wastewater from electronic component manufacturing unit with electroplating were evaluated for toxicity using V. fischeri bioassay. The majority (88%) of tested municipal wastewater samples were found to be highly toxic (EC50) in summer as compared to monsoon season...
Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture, 2019
Plant growth-promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylate deaminase (ACC... more Plant growth-promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity facilitate the improvement of agricultural productivity and yield stability. The present work reports the efficiency of Enterobacter cloacae with ACCD activity isolated from an organic farm near SHUATS, Allahabad, India. This PGPR was screened for stimulation of seed germination and growth promotion of the rice var. Sahbhagi in the presence of ammonium sulphate as a substitute of 1-aminocyclopropane-1-carboxylate (ACC). Inoculation of rice var. Sahbhagi with the PGPR showed a significant (p < 0.01) increase in seed germination and enhancement in elongation of root and shoot compared to untreated control. Potential efficacy of E. cloacae (KP226569) to enhance the growth of rice will be discussed.
Skin is the semipermeable filtration system having many dynamic interactions with microbes that r... more Skin is the semipermeable filtration system having many dynamic interactions with microbes that reside on skin surface. Many experiments had revealed the mutual beneficial relationship of these bacteria with the host (Cogen et al., 2008) . The most direct benefit is the production of lysozyme, acidity (pH 4.5-5.9) which impart antifungal and antimicrobial properties, and defensins (HBD-2,HBD-3), antimicrobial peptide marker LL-37, HBD-2,HBD3,lantibiotics, and other molecules that act to resist co-colonization by pathogens(Cogen et al., 2010; Nakatsuji et al., 2013; Lai et al., International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 2 (2020) Journal homepage: http://www.ijcmas.com
During sugar and alcohol production, large amounts of waste and wastewater are produced. These ma... more During sugar and alcohol production, large amounts of waste and wastewater are produced. These may have a considerable environmental impact by polluting both water bodies and soil, by causing an adverse climatic effect and odour. Due to the high concentration of organic matter, both distillery waste and wastewater at the same time do have a great nutrient and energy potential that can be utilized for fertilizing or power generating purposes. The water can principally be reused for irrigation purposes. Waste also can be used as compost in agriculture.
Sustainable agriculture and environmental protection have the foremost importance in the welfare ... more Sustainable agriculture and environmental protection have the foremost importance in the welfare of human being. Annually, agro-wastes are generated at the millions of tonnes scale worldwide that must be degraded in terms of valuable products as well as the concept of sustainable agriculture can also be implemented through the bioconversion of agro residue into other resources without harming and depleting the natural ecosystem. Microbes are a crucial player for the conversion of agro-waste into valuable products, extraction of minerals, enhancement of agriculture, and agro-waste management. So the use of microorganisms with different biotechnological approaches is the most effective method to treat different wastes, in addition to being eco-friendly, cost-effective, and
This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in... more This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in lentil (Lens culinaris L.) crop, in the department of microbiology . In this experiment five treatment were taken as Un-inoculated (T1), Rhizobium (T2), PSB + Rhizobium (T3), VAM + Rhizobium (T4) and Rhizobium + PSB + VAM (T5). The experiment was carried out during Rabi 2010 in 2.0 m plot size at pot culture house of the Department of Soil Science and Agricultural Chemistry, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur. The result of this study revealed that the maximum active nodulation at 30, 60 and 90 days (8.80 13.50 and 5.00), test weight (32 g/1000 seeds), grain yield (23.8 g/ microplot) and straw yield (26.20 g/ microplot) was found in Rhizobium + PSB + VAM (T5) fallowed by PSB + Rhizobium (T3), VAM + Rhizobium (T4) and Rhizobium (T2) whereas minimum was noticed Uninoculated (T1) microplot. It was recommended that interactive use of Rhizobium + PSB + VAM ...
Soil saltiness is a noteworthy issue as it results in loss of profitability and development of ag... more Soil saltiness is a noteworthy issue as it results in loss of profitability and development of agrarian harvests and decline in soil health. Microorganisms associated with plants contribute to their growth promotion and salinity tolerance by employing a multitude of macromolecules and pathways. Plant growth promoting rhizobacteria (PGPR) have an immediate impact on improving profitability based on higher crop yield. Some PGPR produce 1-aminocyclopropane-1-carboxylic (ACC) deami- nase (EC 4.1.99.4), which controls ethylene production by diverting ACC into α-ketobutyrate and ammonia. ACC deaminase enhances germination rate and growth parameters of root and shoot in different harvests with and without salt stress. Arbuscular mycorrhizal fungi (AMF) show a symbiotic relationship with plants, which helps in efficient uptake of mineral nutrients and water by the plants and also provide protection to the plants against pathogens and various abiotic stresses. The dual inoculation of PGPR an...
International Journal of Current Microbiology and Applied Sciences, Dec 10, 2017
Organic farming is an eco-friendly agro ecosystem that helps maintain soil health in terms of bio... more Organic farming is an eco-friendly agro ecosystem that helps maintain soil health in terms of biological fertility and productivity and bacterial communities in soil play an essential role. A Model Organic Farm (MOF), developed in 2002 to demonstrate organic farming modules among the farming community, is utilized to study bacterial composition, diversity, seasonal variations, and plant growth promotion (PGP) traits of the soil. We observe rich bacterial diversity in MOF soil in terms of types and PGP traits. Rich bacterial diversity in MOF soil is represented by heterotrophs, coliforms, Pseudomonas spp., Azotobacter spp. and Rhizobium spp. and majority of these microorganisms display multiple PGP traits. Bacterial isolates are predominately positive to production of ammonia (NH3) (93.2%), indole acetic acid (IAA) (89.6), catalase (85.0), 1aminocyclopropane-1-carboxylate deaminase (ACCD) (78.6%) and siderophore (69.0%). Richness of their functional characteristics is further revealed by tolerance to salinity and wide range of pH. All the isolates are tolerant to > 5 % NaCl and wide range of pH. Furthermore, majority (96-97%) of the bacterial population of nitrogen fixers are Azotobacter spp. and Rhizobium spp. with multiple PGP traits, tolerance to salinity and wide range of pH. The present study demonstrates that organic farming enriches soil fertility and biodiversity, as well as, less dependent on external high inputs. Thus organic farming offers promise of achieving ecological, economic and social stability in food production system.
International Journal of Current Microbiology and Applied Sciences, 2020
The most direct benefit is the production of lysozyme, acidity (pH 4.5-5.9) which impart antifung... more The most direct benefit is the production of lysozyme, acidity (pH 4.5-5.9) which impart antifungal and antimicrobial properties, and defensins (HBD-2,HBD-3), antimicrobial peptide marker LL-37, HBD-2,HBD-3,lantibiotics, and other molecules that act to resist co-colonization by pathogens(
Soil saltiness is a noteworthy issue as it results in loss of profitability and development of ag... more Soil saltiness is a noteworthy issue as it results in loss of profitability and development of agrarian harvests and decline in soil health. Microorganisms associated with plants contribute to their growth promotion and salinity tolerance by employing a multitude of macromolecules and pathways. Plant growth promoting rhizobacteria (PGPR) have an immediate impact on improving profitability based on higher crop yield. Some PGPR produce 1-aminocyclopropane-1-carboxylic (ACC) deaminase (EC 4.1.99.4), which controls ethylene production by diverting ACC into α-ketobutyrate and ammonia. ACC deaminase enhances germination rate and growth parameters of root and shoot in different harvests with and without salt stress. Arbuscular mycorrhizal fungi (AMF) show a symbiotic relationship with plants, which helps in efficient uptake of mineral nutrients and water by the plants and also provide protection to the plants against pathogens and various abiotic stresses. The dual inoculation of PGPR and ...
Lipoxygenases by generating pro-inflammatory mediators called leukotriens or anti-inflammatory me... more Lipoxygenases by generating pro-inflammatory mediators called leukotriens or anti-inflammatory mediators called lipoxins are involved in the regulation of several inflammatory responses. They are knows as a group of oxidative enzymes along with a non-heme iron atom in their active sites. We were focused on expression pattern of arachidonic lipoxygenase genes to investigate which of the LOX gene in human plays most versatile role in different and tissues and cell lines so that consensus can be established about this protein family and more research can pay more attention. In this in-silico study we analyzed the different lipoxygenase gene expression patterns in different tissues and cell lines to address issues related to lipoxygenase in several inflammatory disease and cancer. Tissue and cell line datasets of for all lipoxygenase genes were retrieved from atlas and Human protein atlas databases. Further data were analyzed and visualized using R package. ALOX5 and ALOX5AP are highly ...
Agriculture is the best foundation for human livelihoods, and, in this respect, crop production h... more Agriculture is the best foundation for human livelihoods, and, in this respect, crop production has been forced to adopt sustainable farming practices. However, soil salinity severely affects crop growth, the degradation of soil quality, and fertility in many countries of the world. This results in the loss of profitability, the growth of agricultural yields, and the step-by-step decline of the soil nutrient content. Thus, researchers have focused on searching for halotolerant and plant growth-promoting bacteria (PGPB) to increase soil fertility and productivity. The beneficial bacteria are frequently connected with the plant rhizosphere and can alleviate plant growth under salinity stress through direct or indirect mechanisms. In this context, PGPB have attained a unique position. The responses include an increased rate of photosynthesis, high production of antioxidants, osmolyte accumulation, decreased Na+ ions, maintenance of the water balance, a high germination rate, and well-d...
Plant growth promoting bacteria (PGPB) play an important role in agricultural production and soil... more Plant growth promoting bacteria (PGPB) play an important role in agricultural production and soil fertility. Wheat is a highly consumed cereal food crop of the world population and sustainable wheat productivity achieved by the application of bacterium in combination with NPK is promising. In the present study bacterial isolate (PR6) from soil of organic farm was included. The isolate (PR6) was screened for its morphological, biochemical and plant growth promoting characteristics, sequenced by 16S rDNA method and submitted to NCBI for the confirmation of strain identification. Further, the inoculation effect of the bacterial isolate in combination with NPK on growth and yield parameters of wheat var. AAI-W6 were analysed. The isolate (PR6) was identified as Erwinia sp. with NCBI Accession No. KP226572. The organism possessed multiple plant growth promoting (MPGP) traits such as production of ammonia, siderophore, indole-3-acetic acid (IAA), ACC Deaminase (ACCD) and showed phosphate ...
This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in... more This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in lentil (Lens culinaris L.) crop, in the department of microbiology . In this experiment five treatment were taken as Un-inoculated (T1), Rhizobium (T2), PSB + Rhizobium (T3), VAM + Rhizobium (T4) and Rhizobium + PSB + VAM (T5). The experiment was carried out during Rabi 2010 in 2.0 m plot size at pot culture house. The result of this study revealed that the least nitrogen content in grain and straw was found in uninoculated micro plat (T1) whereas maximum nitrogen content was noticed in Rhizobium + PSB + VAM treated field (T5). Lowest phosphorous content in grain and straw was found in uninoculated micro plat (T1) whereas maximum nitrogen content was noticed in Rhizobium + PSB + VAM treated field (T5). Least nitrogen uptake in grain and straw was found in uninoculated micro plat (T1) whereas maximum nitrogen uptake was noticed in Rhizobium + PSB + VAM treated field (T5). The lowest phos...
Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture, 2019
Recent genome sequencing of Trichoderma species has opened the way for their diversity in the ass... more Recent genome sequencing of Trichoderma species has opened the way for their diversity in the assessment and comparative analysis of microsatellites. Here, we compared microsatellites of three taxonomically different Trichoderma species such as T. asperellum, T. citrinoviride, and T. longibrachiatum. Our results highlight abundance and diversity of microsatellite patterns in all the test genomes. The highest relative abundance (283.6) with a relative density (3424.7) of SSRs was identified in all three sequence sets of T. citrinoviride. Among all three sequence sets, selection of these isolates were based on maximum frequency of SSRs which was of trinucleotide repeats (79.8%), whereas the dinucleotide repeat represented <8%. With the help of bioinformatics tools, species-specific diagnostic primers were developed and validated by silica PCR. Out of 31 primer sets, only 18 pairs of primer indicated successful amplification among all the species tested. A total of 34 alleles were d...
International journal of ecology and environmental sciences, 2019
Increased industrialization and urbanization created ecological and toxicological problems from t... more Increased industrialization and urbanization created ecological and toxicological problems from the release of toxic contaminants into the environment. Conventional toxicity bioassays that use animals are time consuming and expensive. The V. fischeri bioassay in which the inhibition of light emission by V. fischeri is considered a rapid, sensitive and simple test for toxicity as compared to other biological methods and assays using algae, protozoa and fish which require 7-10 days. In the present study the suitability of V. fischeri bioassay for the evaluation of toxicity of municipal and industrial wastewater in Allahabad (India) was conducted. Twenty five municipal wastewater streams in city of Allahabad and industrial wastewater from electronic component manufacturing unit with electroplating were evaluated for toxicity using V. fischeri bioassay. The majority (88%) of tested municipal wastewater samples were found to be highly toxic (EC50) in summer as compared to monsoon season...
Plant Growth Promoting Rhizobacteria (PGPR): Prospects for Sustainable Agriculture, 2019
Plant growth-promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylate deaminase (ACC... more Plant growth-promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity facilitate the improvement of agricultural productivity and yield stability. The present work reports the efficiency of Enterobacter cloacae with ACCD activity isolated from an organic farm near SHUATS, Allahabad, India. This PGPR was screened for stimulation of seed germination and growth promotion of the rice var. Sahbhagi in the presence of ammonium sulphate as a substitute of 1-aminocyclopropane-1-carboxylate (ACC). Inoculation of rice var. Sahbhagi with the PGPR showed a significant (p < 0.01) increase in seed germination and enhancement in elongation of root and shoot compared to untreated control. Potential efficacy of E. cloacae (KP226569) to enhance the growth of rice will be discussed.
Skin is the semipermeable filtration system having many dynamic interactions with microbes that r... more Skin is the semipermeable filtration system having many dynamic interactions with microbes that reside on skin surface. Many experiments had revealed the mutual beneficial relationship of these bacteria with the host (Cogen et al., 2008) . The most direct benefit is the production of lysozyme, acidity (pH 4.5-5.9) which impart antifungal and antimicrobial properties, and defensins (HBD-2,HBD-3), antimicrobial peptide marker LL-37, HBD-2,HBD3,lantibiotics, and other molecules that act to resist co-colonization by pathogens(Cogen et al., 2010; Nakatsuji et al., 2013; Lai et al., International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 2 (2020) Journal homepage: http://www.ijcmas.com
During sugar and alcohol production, large amounts of waste and wastewater are produced. These ma... more During sugar and alcohol production, large amounts of waste and wastewater are produced. These may have a considerable environmental impact by polluting both water bodies and soil, by causing an adverse climatic effect and odour. Due to the high concentration of organic matter, both distillery waste and wastewater at the same time do have a great nutrient and energy potential that can be utilized for fertilizing or power generating purposes. The water can principally be reused for irrigation purposes. Waste also can be used as compost in agriculture.
Sustainable agriculture and environmental protection have the foremost importance in the welfare ... more Sustainable agriculture and environmental protection have the foremost importance in the welfare of human being. Annually, agro-wastes are generated at the millions of tonnes scale worldwide that must be degraded in terms of valuable products as well as the concept of sustainable agriculture can also be implemented through the bioconversion of agro residue into other resources without harming and depleting the natural ecosystem. Microbes are a crucial player for the conversion of agro-waste into valuable products, extraction of minerals, enhancement of agriculture, and agro-waste management. So the use of microorganisms with different biotechnological approaches is the most effective method to treat different wastes, in addition to being eco-friendly, cost-effective, and
This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in... more This study was conducted to evaluate the effect of biofertilizers viz. Rhizobium , PSB and VAM in lentil (Lens culinaris L.) crop, in the department of microbiology . In this experiment five treatment were taken as Un-inoculated (T1), Rhizobium (T2), PSB + Rhizobium (T3), VAM + Rhizobium (T4) and Rhizobium + PSB + VAM (T5). The experiment was carried out during Rabi 2010 in 2.0 m plot size at pot culture house of the Department of Soil Science and Agricultural Chemistry, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur. The result of this study revealed that the maximum active nodulation at 30, 60 and 90 days (8.80 13.50 and 5.00), test weight (32 g/1000 seeds), grain yield (23.8 g/ microplot) and straw yield (26.20 g/ microplot) was found in Rhizobium + PSB + VAM (T5) fallowed by PSB + Rhizobium (T3), VAM + Rhizobium (T4) and Rhizobium (T2) whereas minimum was noticed Uninoculated (T1) microplot. It was recommended that interactive use of Rhizobium + PSB + VAM ...
Soil saltiness is a noteworthy issue as it results in loss of profitability and development of ag... more Soil saltiness is a noteworthy issue as it results in loss of profitability and development of agrarian harvests and decline in soil health. Microorganisms associated with plants contribute to their growth promotion and salinity tolerance by employing a multitude of macromolecules and pathways. Plant growth promoting rhizobacteria (PGPR) have an immediate impact on improving profitability based on higher crop yield. Some PGPR produce 1-aminocyclopropane-1-carboxylic (ACC) deami- nase (EC 4.1.99.4), which controls ethylene production by diverting ACC into α-ketobutyrate and ammonia. ACC deaminase enhances germination rate and growth parameters of root and shoot in different harvests with and without salt stress. Arbuscular mycorrhizal fungi (AMF) show a symbiotic relationship with plants, which helps in efficient uptake of mineral nutrients and water by the plants and also provide protection to the plants against pathogens and various abiotic stresses. The dual inoculation of PGPR an...
International Journal of Current Microbiology and Applied Sciences, Dec 10, 2017
Organic farming is an eco-friendly agro ecosystem that helps maintain soil health in terms of bio... more Organic farming is an eco-friendly agro ecosystem that helps maintain soil health in terms of biological fertility and productivity and bacterial communities in soil play an essential role. A Model Organic Farm (MOF), developed in 2002 to demonstrate organic farming modules among the farming community, is utilized to study bacterial composition, diversity, seasonal variations, and plant growth promotion (PGP) traits of the soil. We observe rich bacterial diversity in MOF soil in terms of types and PGP traits. Rich bacterial diversity in MOF soil is represented by heterotrophs, coliforms, Pseudomonas spp., Azotobacter spp. and Rhizobium spp. and majority of these microorganisms display multiple PGP traits. Bacterial isolates are predominately positive to production of ammonia (NH3) (93.2%), indole acetic acid (IAA) (89.6), catalase (85.0), 1aminocyclopropane-1-carboxylate deaminase (ACCD) (78.6%) and siderophore (69.0%). Richness of their functional characteristics is further revealed by tolerance to salinity and wide range of pH. All the isolates are tolerant to > 5 % NaCl and wide range of pH. Furthermore, majority (96-97%) of the bacterial population of nitrogen fixers are Azotobacter spp. and Rhizobium spp. with multiple PGP traits, tolerance to salinity and wide range of pH. The present study demonstrates that organic farming enriches soil fertility and biodiversity, as well as, less dependent on external high inputs. Thus organic farming offers promise of achieving ecological, economic and social stability in food production system.
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Papers by Dr. Alka Sagar