Papers by Danuta Kierek-Jaszczuk
Letters in Applied Microbiology, 1995
A s p e r g i h s ochraceus Wilhelm is a producer of highly toxic mycotoxin, ochratoxin A, and is... more A s p e r g i h s ochraceus Wilhelm is a producer of highly toxic mycotoxin, ochratoxin A, and is known to contribute to the damage of grain on storage. Rabbit antibodies were produced against the exoantigens of A. ochraceus, and ELISA and immunoblotting procedures were used to characterize their reactivity towards fungal exoantigens of four Aspergillus, seven Penicillium, three Fusarium, one Mucor and one Alternaria species. T h e antibodies were specific for A. ochraceus and essentially cross-reacted neither with other fungi nor with the water-soluble grain components. These antibodies, therefore, have the potential of being utilized for the discriminative monitoring of A. ochraceus in foodstuffs and feeds.
PubMed, 1982
The effect of heating, urea and L-homoarginine on the total activity of serum alkaline phosphatas... more The effect of heating, urea and L-homoarginine on the total activity of serum alkaline phosphatase was investigated in pigs of Akp B, Akp BC and Akp A phenotypes. Each of these factors decreased the activity of the enzyme, but under the experimental conditions applied the effect exerted by L-homoarginine was weakest. Statistically significant differences were demonstrated in the degree of action of each factor in different phenotypes of the animals. It was observed that urea as well as heating decreased the total activity of serum alkaline phosphatase in B-phenotype animals more than in animals of BC and AB phenotypes.
PubMed, Jul 1, 1980
Qualitative and quantitative investigations of serum alkaline phosphatase were carried out in mat... more Qualitative and quantitative investigations of serum alkaline phosphatase were carried out in maturing piglets. It was found that with progressing maturation of the animals the activity of the enzyme decreased and its phenotype changed. It has been demonstrated that presence of the fast fraction of alkaline phosphatase causes a significantly greater activity of the enzyme. It has been demonstrated also that in each age group differences in the activity of the enzyme observed between litters were significantly higher than those observed within litters.
PubMed, Sep 1, 1980
The activity of serum alkaline phosphatase in pigs changes in relation to the age of the animals ... more The activity of serum alkaline phosphatase in pigs changes in relation to the age of the animals [2, 4, 5, 7]. In chicken [3] it was observed that the activity of alkaline phosphatase showed cyclic fluctuations during the process of egg formation, but in the 24 hours day cyclic the activity of the enzyme was not increasing as far as the process of shell calcification was not taking place. In the presently reported work circadian fluctuations of alkaline phosphatase activity were investigated in the serum of piglets.
Journal of Food Protection, May 1, 1996
The study describes procedures that were used to develop a highly sensitive enzyme-linked immunos... more The study describes procedures that were used to develop a highly sensitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of a trichothecene mycotoxin, 3-acetyldeoxynivalenol (3-AcDON), in barley. Polyclonal antibodies were produced in rabbits immunized with a conjugate of 3-AcDON and human serum albumin linked by an ester linkage (hemisuccinate bridge). High anti-3-AcDON titers were obtained after multiple immunizations. However, only a negligible degree of inhibition was obtained with 3-AcDON in a competitive ELISA when the coating conjugate contained the same ester linkage group (hemiglutarate bridge) as the immunogen. The use of a conjugate containing a heterologous ether linkage (O-methylcarboxyl bridge) compared to that of the immunogen yielded an inhibition curve for 3-AcDON that was highly sensitive (ICso = 0.21 ng/ml) with essentially no interference from the bridging group. This conjugate was synthesized using iodoacetate and l,l'-carbonyldiimidazole chemistries. The assay showed low cross-reactivity with other trichothecenes including several analogs of deoxynivalenol (DON) with the exception of acetylated DON. The ELISA developed on the basis of this new conjugate was able to detect low concentrations of 3-AcDON (16 ppb) in spiked barley without any cleanup treatments.
Human antibodies, Sep 1, 1993
Severe combined immunodeficient (SCID) mice were reconstituted with normal human peripheral blood... more Severe combined immunodeficient (SCID) mice were reconstituted with normal human peripheral blood leukocytes (PBLs) and were shown to produce a human anti-mouse immunoglobulin antibody response on immunization with heat-treated murine monoclonal IgGJ antibody to ovalbumin, referred to as ha-Mab-2. The human anti-mouse antibody response was proportional to the number of B cells and mononuclear cells transferred from a given batch of PBL. However, pretreatment of hu-PBL-SCID mice with a tolerogenic covalent conjugate of monomethoxypolyethylene glycol (mPEG) and Mab-2 suppressed this response on subsequent injections of ha-Mab-2, and this suppression was shown to be antigen-specific, i.e., it did not suppress the antibody response to ovalbumin and did not affect the level of production of human immunoglobulin of hu-PBL-SCID mice. The suppression was due to the generation of human suppressor CD8+ T (Ts) cells, which down regulated CD4+ helper T cells in an antigen and HLA class I specific manner, i.e., these findings were in accord with the previously shown immunosuppressive effect of tolerogenic mPEG conjugates in normal mice.
Cellular Immunology, Aug 1, 1993
Immunology Letters, May 1, 1992
The effectiveness of therapeutically relevant xenogeneic monoclonal antibodies (MoAb) may be coun... more The effectiveness of therapeutically relevant xenogeneic monoclonal antibodies (MoAb) may be counteracted by their inherent immunogenicity. Since conjugates of diverse proteins with mono-methoxypolyethylene glycol (mPEG) were shown to induce Ag-specific tolerance in mice and rats, we used outbred rats in this study as an experimental model for establishing the tolerogenicity of mPEG conjugates of murine MoAb. The results demonstrate that: (i) murine anti-rat CD4 MoAb (W3/25) were more immunogenic than murine anti-human CD4 MoAb (MAX.16H5) in rats; (ii) W3/25 preferentially induced an anti-idiotypic (anti-id) antibody response; and (iii) antibodies to both common and idiotypic determinants could be suppressed in rats by treatment with W3/25(mPEG)28.
Intervirology, 1986
Common antigenic properties for p85 and p75 but a different antigenic character for p71 Aleutian ... more Common antigenic properties for p85 and p75 but a different antigenic character for p71 Aleutian disease virus (ADV) proteins were demonstrated by Western blot analysis with monoclonal antibodies. It was shown that four hybridomas (ADV-Hy 47, 66, 77 and 84) with specific reactivity for structural proteins p85 and p75 also recognized p25 but not the p71, nonstructural, protein. In turn, the monoclonal antibody ADV-Hy 2 recognized the p71 protein only. For further studies of their antigenic properties, the ADV proteins were subjected to enzymatic or chemical cleavage. The derived peptide fragments were analyzed by epitopic mapping. Depending on the cleavage reagent and monoclonal antibody applied, specific peptide maps were revealed. The maps of p85 and p75 were very similar, indicating that both proteins shared an extensive antigenic relationship. After cleavage with alpha-chymotrypsin and N-chlorosuccinimide and by using the ADV-Hy 84 monoclonal antibody, unique peptide fragments were identified with p85 which had no counterparts in p75 fragments.
Human Antibodies, 1993
Severe combined immunodeficient (SCID) mice were reconstituted with normal human peripheral blood... more Severe combined immunodeficient (SCID) mice were reconstituted with normal human peripheral blood leukocytes (PBLs) and were shown to produce a human anti-mouse immunoglobulin antibody response on immunization with heat-treated murine monoclonal IgGJ antibody to ovalbumin, referred to as ha-Mab-2. The human anti-mouse antibody response was proportional to the number of B cells and mononuclear cells transferred from a given batch of PBL. However, pretreatment of hu-PBL-SCID mice with a tolerogenic covalent conjugate of monomethoxypolyethylene glycol (mPEG) and Mab-2 suppressed this response on subsequent injections of ha-Mab-2, and this suppression was shown to be antigen-specific, i.e., it did not suppress the antibody response to ovalbumin and did not affect the level of production of human immunoglobulin of hu-PBL-SCID mice. The suppression was due to the generation of human suppressor CD8+ T (Ts) cells, which down regulated CD4+ helper T cells in an antigen and HLA class I specific manner, i.e., these findings were in accord with the previously shown immunosuppressive effect of tolerogenic mPEG conjugates in normal mice.
Journal of Food Protection, 1997
Anti- T-2 toxin (T-2) antibodies raised in chicken hosts were isolated as total IgY (chicken immu... more Anti- T-2 toxin (T-2) antibodies raised in chicken hosts were isolated as total IgY (chicken immunoglobulins of IgY isotype) from serum and the egg yolk. They were used for quantitation of T-2 toxin in an indirect competitive enzyme-linked immunosorbent assay (ELISA) which employed molecularly distinct toxin-carrier conjugates as solid-phase assay antigens. The sensitivities of the assays utilizing any of the purified IgY or unfractionated serum were similar but varied with respect to the type of conjugate used and could be improved by lowering the concentration of assay antigens or antibodies. Low concentrations of both immunoreactants resulted in assays of superb sensitivity, although the standard curves generated with some assay antigens decreased markedly in slopes and impractically long incubation times were required to develop sufficiently high read-out signals. However, high optical signals were obtained in a very short period of time when an amplified substrate system instea...
Journal of Food Protection, 1996
The study describes procedures that were used to develop a highly sensitive enzyme-linked immunos... more The study describes procedures that were used to develop a highly sensitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of a trichothecene mycotoxin, 3-acetyldeoxynivalenol (3-AcDON), in barley. Polyclonal antibodies were produced in rabbits immunized with a conjugate of 3-AcDON and human serum albumin linked by an ester linkage (hemisuccinate bridge). High anti-3-AcDON titers were obtained after multiple immunizations. However, only a negligible degree of inhibition was obtained with 3-AcDON in a competitive ELISA when the coating conjugate contained the same ester linkage group (hemiglutarate bridge) as the immunogen. The use of a conjugate containing a heterologous ether linkage (O-methylcarboxyl bridge) compared to that of the immunogen yielded an inhibition curve for 3-AcDON that was highly sensitive (IC50 = 0.21 ng/ml) with essentially no interference from the bridging group. This conjugate was synthesized using iodoacetate and 1,1′-carbonyldiimidazole chemi...
American clinical laboratory
Animal Blood Groups and Biochemical Genetics, 2009
SummaryGenetic variants of serum alkaline phosphatase were studied by the method of starch gel el... more SummaryGenetic variants of serum alkaline phosphatase were studied by the method of starch gel electrophoresis in the Zlotnicka Pstra breed of pigs. Two regions of alkaline phosphatase migration were observed. A single fraction in region I and four different phenotypes: AB, B, BC and BD in region II, were found. For AB, B and BC phenotypes the genetic control by three alleles AkpA, AkpB and AkpC in suggested. The observed segregation ratios in some cases deviated significantly from the expected ones.
Letters in Applied Microbiology, 1995
A s p e r g i h s ochraceus Wilhelm is a producer of highly toxic mycotoxin, ochratoxin A, and is... more A s p e r g i h s ochraceus Wilhelm is a producer of highly toxic mycotoxin, ochratoxin A, and is known to contribute to the damage of grain on storage. Rabbit antibodies were produced against the exoantigens of A. ochraceus, and ELISA and immunoblotting procedures were used to characterize their reactivity towards fungal exoantigens of four Aspergillus, seven Penicillium, three Fusarium, one Mucor and one Alternaria species. T h e antibodies were specific for A. ochraceus and essentially cross-reacted neither with other fungi nor with the water-soluble grain components. These antibodies, therefore, have the potential of being utilized for the discriminative monitoring of A. ochraceus in foodstuffs and feeds.
International Archives of Allergy and Immunology, 1993
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Papers by Danuta Kierek-Jaszczuk