Papers by Danijela Pecarski
Antioxidants
This study aimed to examine the effects of a 14-day treatment with lady’s bedstraw methanol extra... more This study aimed to examine the effects of a 14-day treatment with lady’s bedstraw methanol extract on doxorubicin-induced cardiotoxicity through functional, biochemical and histological examinations. We used 24 male Wistar albino rats divided into the following groups: control (CTRL), doxorubicin (DOX), and DOX + GVE (Galium verum extract). GVE was administered orally at a dose of 50 mg/kg per day for 14 days, while a single dose of doxorubicin was injected into the DOX groups. After accomplishing treatment with GVE, cardiac function was assessed, which determined the redox state. During the autoregulation protocol on the Langendorff apparatus, ex vivo cardiodynamic parameters were measured. Our results demonstrated that the consumption of GVE effectively suppressed the disturbed response of the heart to changes in perfusion pressures caused by administration of DOX. Intake of GVE was associated with a reduction in most of the measured prooxidants in comparison to the DOX group. Mo...
Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Bulletin
Purpose This study aimed in exploitation of lignocellulosic wastes for the evaluation of the newl... more Purpose This study aimed in exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential, covered by the circular economy frame.Methods A standard microbiological methods for the isolation of the white-rot fungal mycelia were used, followed by DNA extraction, PCR amplification, and the API ZYM test. The determination of laccase activity was based on the oxidation of guaiacol while the DNS method was used for the hydrolase determination.Results The isolates, belonged to Basidiomycetes, Fomes fomentraius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for Carboxymethyl cellulase and Avicelase production. The strain TMF1 was able to produce...
E-business technologies conference proceedings, Sep 24, 2021
Macedonian Pharmaceutical Bulletin, 2020
Biomass Conversion and Biorefinery, 2022
SAAs (Surface Active Agents), surfactants or tensides, are amphiphilic substances of organic natu... more SAAs (Surface Active Agents), surfactants or tensides, are amphiphilic substances of organic nature, which have the ability to be absorbed on the surface of particles, changing their surface tension. Thanks to their amphiphilic, polar-nonpolar dual nature, one part of their molecule is soluble in a particular liquid (the lyophilic part), whereas the other part is not soluble (the lyophobic part). The polar part of a molecule is called the head and is hydrophilic, whereas the nonpolar part is the hydrophobic tail soluble in fats. SAAs are substances present in washing agents, face and body care products, medications and parapharmaceuticals, food products, as well as textile, paint and oil production, and in many other products. Those SAAs have different roles: moisturizers, emulsifiers, solubilizers, foamers/antifoamers, rheologic modifiers, antistatic agents, lubricants (Schramm et al., 2003) so, they have a role of a detergent in cosmetic preparation (shampoos, liquid soaps); in co...
Journal of the Serbian Chemical Society, 2021
A recent trend in sustainable bioethanol production is the use of agricultural waste or food wast... more A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24?72 h) and waste brewer?s yeast inoculum (1?4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hy...
Environmental Technology & Innovation, 2021
Abstract During the last few decades, energy demand is increasing rapidly. Concerning this, the u... more Abstract During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables — lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste — molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h ( ∼ 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h ( ∼ 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer’s yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production.
Acta Poloniae Pharmaceutica - Drug Research
Aging of the skin is a complex and multifactorial biological process, affected by different intri... more Aging of the skin is a complex and multifactorial biological process, affected by different intrinsic and extrinsic factors. Exogenous factors affecting the process of skin aging, together with nutrition, are topics which currently draw considerable attention. Although it is difficult to accurately define what presents a healthy diet for maintenance of youthful skin, considering the evident lack of clinical data and studies in humans, there are numerous evidence suggesting that the food we eat affects our skin aging and its’ appearance. Well-documented is a fact that the modern diet is a large source of advanced glycation end products and reactive oxygen species – compounds formed during certain food preparation methods which accumulation is associated with different disease and recently, skin aging as well. This review gives the current state of knowledge related to the role which advanced glycation end products and reactive oxygen species, formed during the certain food preparatio...
Hemijska Industrija, 2014
Acta periodica technologica, 2014
Carbohydrate Polymers, 2004
Vojnosanitetski pregled, 2016
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Papers by Danijela Pecarski