Papers by Directive Publications
Annals of Urology, 2024
Objective : Men with multiple sclerosis (MS) tend to present with urinary symptoms potentially tr... more Objective : Men with multiple sclerosis (MS) tend to present with urinary symptoms potentially translating to significant findings on urodynamics (UDS). We sought to assess the reliability and prognostic value of the International Prostate Symptom Score (IPSS) and Quality of Life (QoL) in patients with MS. Methods : A prospective study between July 2014 and August 2016 included 40 consecutive men with MS and lower urinary tract symptoms (LUTs) referred for video UDS. All participants completed the IPSS and QoL assessments. Results were compared and correlated to UDS parameters. Results : Mean (±SD) age, IPSS, and QoL were 53.7 ± 13.5 years, 16.7 ± 8.9 and 4.1 ± 1.4, respectively. The most common presenting symptom and UDS finding were urinary frequency (79%) and detrusor overactivity (68%). 72% had moderate-severe symptoms base on the IPSS. Reduced bladder compliance on UDS was associated with intermittency and straining answers on IPSS (p = 0.02 and p = 0.03). QoL did not predict UDS parameters. Conclusion : QoL and total IPSS scores are likely insufficient for MS and LUTs patient assessment due to the variability of UDS findings. However, in these patients, IPSS storage symptom questions had significant correlation with UDS.
The Journal of Nephrology, 2024
Background : Peritonitis remains the most feared complication of End Stage Renal Disease (ESRD) p... more Background : Peritonitis remains the most feared complication of End Stage Renal Disease (ESRD) patients on peritoneal dialysis (PD), but cultures are often negative. Our study was aimed to determine if improving the collection techniques of peritoneal fluid would reduce the rates of Culture Negative Peritonitis (CNP) as recommended in the 2020 ISPD guidelines.
Journal of Dentistry and Oral Research, 2024
Objective : To study the effect of composite resin monomer BisGMA on human dental pulp stem cell ... more Objective : To study the effect of composite resin monomer BisGMA on human dental pulp stem cell (hDPSCs) odontoblasts differentiation, and explore the effect of composite resin filling teeth on dentin formation. Material and Methods : separate and culture hDPSCs, identify cell phenotype by flow cytometry, and induce staining to assess multidirectional differentiation potential. The hDPSCs was stimulated with different concentrations of monomeric BisGMA, and the odontoblast differentiation capacity of each group was determined by CCK-8 assay, alkaline phosphatase activity detection, and q-PCR. Results: CCK-8 showed that the addition of 0.5 mM-8 mM BisGMA affected the cell increment, but no significant difference; the concentration of 0.5 mM-8 mM BisGMA reduced the cellular alkaline phosphatase activity and further decreased the activity with increasing concentration.The q-PCR results also showed that monomeric BisGMA inhibited the dentin-related gene OC (osteocalcin), DSPP (dentin sialophosphoprotein)Expression, and with the concentration increases, the inhibition is more obvious of. Conclusion : Composite resin monomer BisGMA has a negative effect on odontogenic differentiation of human dental pulp stem cells.
Clinical Imaging and Case Reports, 2024
Purpose : To compare clinical outcomes among patients treated with comprehensive and non-comprehe... more Purpose : To compare clinical outcomes among patients treated with comprehensive and non-comprehensive radiation fields for squamous cell carcinoma of occult primary origin involving the cervical lymph nodes.
Journal of Nutrition and Food Science Research, 2024
Developing foods with boosted functionality to minimize the potential side effects of oxidative/n... more Developing foods with boosted functionality to minimize the potential side effects of oxidative/nitrosative stress and support the immune system while responding to consumers' needs and preferences is a challenging trend in the food industry. The use of Moringa oleifera leaves, characterized by their high nutritional value and multiple pharmacological properties, is emerging in food applications. The present study aimed at developing a functional ready-to-serve apple-leaf extract was found to be a suitable natural preservative and a functional component with health-promoting properties that could support the body's immune system.
Journal of Nutrition and Food Science Research, 2024
This study presents findings from a cross-sectional survey conducted among mothers and caregivers... more This study presents findings from a cross-sectional survey conducted among mothers and caregivers of children under 5 in Kaduna State, Nigeria. Out of the 1647 collected questionnaires, 77.5% were validated for analysis. Results reveal that 53.3% of the women were aged 20-29 years, with older adolescent mothers (18-19 years) comprising 7.8%. The majority were married (97.8%), and over two-thirds had at least primary education (69.5%). While 54.5% identified as housewives, 28.7% engaged in petty trading, and 12.3% pursued farming full-time. Table 2 in the appendix indicates good knowledge of exclusive breastfeeding (EBF) practices, with 88.4% knowing breastfeeding should start immediately after birth. However, EBF prevalence was only 33.3%, higher among women with more than secondary education (43.2%) and those aged 20-29 years (35.4%). Positive perceptions toward EBF were evident, but misconceptions persisted, impacting practice. Social norms influenced some contradictory behaviors, such as early introduction of water (51%). Self-efficacy emerged as a strong driver, with 86% confident in making feeding decisions. Barriers to EBF included age and social norms, while perception and self-efficacy were identified as drivers. The logistic regression model explained 50.3% of EBF variance, emphasizing the need for targeted interventions. Despite good knowledge, the suboptimal EBF prevalence underscores the importance of addressing multifaceted barriers to enhance breastfeeding practices.
The Journal of Nephrology, 2024
Background : Peritonitis remains the most feared complication of End Stage Renal Disease (ESRD) p... more Background : Peritonitis remains the most feared complication of End Stage Renal Disease (ESRD) patients on peritoneal dialysis (PD), but cultures are often negative. Our study was aimed to determine if improving the collection techniques of peritoneal fluid would reduce the rates of Culture Negative Peritonitis (CNP) as recommended in the 2020 ISPD guidelines.
Methods : We implemented a policy to standardize the collection techniques of PD effluent by introducing an additional step thatincreased the concentration of organisms in the PD effluent. This additional step consisted of culturing resuspended pellet obtained from 50 cc of centrifuged peritoneal fluid onto agar plates in addition to routinely culturing 5-10 cc of their peritoneal fluid in aerobic and anerobic blood culture bottles. We analyzed the differences in the rates of CNP from 01/01/2009 to 07/30/2013 representing patients prior to the new policy and compared it the CNP rates from 08/01/2013 to 12/31/2018 representing patients in the post policy period.
Results : We noticed a remarkable decline in the number of CNP rate from 0.40 (CI 95% 0.37-0.42) to 0.20 (CI 95% (0.19- 0.22) and a total decline in all cases of peritonitis from 0.87 (CI 95% 0.83-0.92) to 0.24 (CI 95% 0.22-0.25) in the post policy period even though patients in the post policy period had more comorbidities [60.9% versus 94.1%], p=0.02. In both the eras, most patients were black and of female gender.
Conclusion : Our study validated the effectiveness of the new PD fluid culture policy based on the 2010 ISPD guidelines.
Directive Publications, 2024
Background: Primary defects in innate immunity in chronic hepatitis-C and-B may shift towards dys... more Background: Primary defects in innate immunity in chronic hepatitis-C and-B may shift towards dysregulated adaptive immunity. Cytokines are critical immune regulators. Aim: To clarify cytokines and chemokines in chronic hepatitis-C and-B to investigate the innate and adaptive immunity. Methods: Twenty-seven serum cytokines were evaluated in 27 patients with chronic hepatitis-C (genotype 1 [n=20], genotype 2 [n=7], hepatitis-C virus. RNA 5.72 ± 3.17 Log IU/ mL),12 patients with chronic hepatitis-B (e-antigen [+] [n=5], e-Ag [-] [n=7], genotype B [n=2], genotype C [n=9], hepatitis-B virus. DNA 6.19 ± 1.31 Log-copies/mL) and controls (n=5). Results: Th1 and Th2 cytokines levels were significantly higher (p< 0.05) in chronic hepatitis-B than those in chronic hepatitis-C. Interleukin-12 and-15 levels were significantly higher (p < 0.05) in chronic hepatitis-B than those in chronic hepatitis C. CXCL8 and-10 were significantly higher (p < 0.05) in chronic hepatitis-C and-B than those in controls. Immune responses were stronger in patients with chronic hepatitis-C (genotype 2) than in those with chronic hepatitis-C (genotype1). The results showed a distinct state of immunity among chronic hepatitis-B and-C. Conclusions: Skewing immunity was observed in chronic hepatitis-B and-C as well as impairments to induce innate immunity linked to adaptive immunity. Augmenting immunological strategies are necessary for the treatment and management of chronic hepatitis-B and-C with a high viral load.
Directive Publications, 2023
Objective : To study the effect of composite resin monomer BisGMA on human dental pulp stem cell ... more Objective : To study the effect of composite resin monomer BisGMA on human dental pulp stem cell (hDPSCs) odontoblasts differentiation, and explore the effect of composite resin filling teeth on dentin formation. Material and Methods : separate and culture hDPSCs, identify cell phenotype by flow cytometry, and induce staining to assess multidirectional differentiation potential. The hDPSCs was stimulated with different concentrations of monomeric BisGMA, and the odontoblast differentiation capacity of each group was determined by CCK-8 assay, alkaline phosphatase activity detection, and q-PCR. Results: CCK-8 showed that the addition of 0.5 mM-8 mM BisGMA affected the cell increment, but no significant difference; the concentration of 0.5 mM-8 mM BisGMA reduced the cellular alkaline phosphatase activity and further decreased the activity with increasing concentration.The q-PCR results also showed that monomeric BisGMA inhibited the dentin-related gene OC (osteocalcin), DSPP (dentin sialophosphoprotein)Expression, and with the concentration increases, the inhibition is more obvious of. Conclusion : Composite resin monomer BisGMA has a negative effect on odontogenic differentiation of human dental pulp stem cells.
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Papers by Directive Publications
Methods : We implemented a policy to standardize the collection techniques of PD effluent by introducing an additional step thatincreased the concentration of organisms in the PD effluent. This additional step consisted of culturing resuspended pellet obtained from 50 cc of centrifuged peritoneal fluid onto agar plates in addition to routinely culturing 5-10 cc of their peritoneal fluid in aerobic and anerobic blood culture bottles. We analyzed the differences in the rates of CNP from 01/01/2009 to 07/30/2013 representing patients prior to the new policy and compared it the CNP rates from 08/01/2013 to 12/31/2018 representing patients in the post policy period.
Results : We noticed a remarkable decline in the number of CNP rate from 0.40 (CI 95% 0.37-0.42) to 0.20 (CI 95% (0.19- 0.22) and a total decline in all cases of peritonitis from 0.87 (CI 95% 0.83-0.92) to 0.24 (CI 95% 0.22-0.25) in the post policy period even though patients in the post policy period had more comorbidities [60.9% versus 94.1%], p=0.02. In both the eras, most patients were black and of female gender.
Conclusion : Our study validated the effectiveness of the new PD fluid culture policy based on the 2010 ISPD guidelines.
Methods : We implemented a policy to standardize the collection techniques of PD effluent by introducing an additional step thatincreased the concentration of organisms in the PD effluent. This additional step consisted of culturing resuspended pellet obtained from 50 cc of centrifuged peritoneal fluid onto agar plates in addition to routinely culturing 5-10 cc of their peritoneal fluid in aerobic and anerobic blood culture bottles. We analyzed the differences in the rates of CNP from 01/01/2009 to 07/30/2013 representing patients prior to the new policy and compared it the CNP rates from 08/01/2013 to 12/31/2018 representing patients in the post policy period.
Results : We noticed a remarkable decline in the number of CNP rate from 0.40 (CI 95% 0.37-0.42) to 0.20 (CI 95% (0.19- 0.22) and a total decline in all cases of peritonitis from 0.87 (CI 95% 0.83-0.92) to 0.24 (CI 95% 0.22-0.25) in the post policy period even though patients in the post policy period had more comorbidities [60.9% versus 94.1%], p=0.02. In both the eras, most patients were black and of female gender.
Conclusion : Our study validated the effectiveness of the new PD fluid culture policy based on the 2010 ISPD guidelines.