Objective: To explore the feasibility of intra-operative cholangiography via the division line of... more Objective: To explore the feasibility of intra-operative cholangiography via the division line of the IV segment bile duct in gallbladder-sparing left lateral hepatic lobectomy for pediatric living-donor liver transplantation. Methods: To perform the gallbladder-sparing left lateral hepatic lobectomy in pediatric living-donor liver transplantation, the liver parenchyma was dissected in the right of falciform ligament, the relative thick IV segment bile duct was dissected and cut off, and the real-time cholangiography was performed through intubation via the proximal bile duct division line. From April 2012 to December 2013, 52 cases of donor were implemented left lateral hepatic lobectomy with this method. According to the result of chloangiography, the type of bile duct and bile duct division line were determined to observe the changes of the donor maximum gallbladder emptying rate. Results: 52 cases of donor intraoperative cholangiography images were clear, with no operation compl...
Background Ischemia and reperfusion injury (IRI) is an ineluctable immune-related pathophysiologi... more Background Ischemia and reperfusion injury (IRI) is an ineluctable immune-related pathophysiological process during organ transplantation, which not only causes a shortage of donor organs, but also has long-term and short-term negative consequences on patients. Severe IRI-induced cell death leads to the release of endogenous substances, which bind specifically to receptors on immune cells to initiate an immune response. Although innate and adaptive immunity have been discovered to play essential roles in IRI in the context of organ transplantation, the pathway and precise involvement of the immune response at various stages has not yet to be elucidated. Methods We combined “IRI” and “organ transplantation” with keywords, respectively such as immune cells, danger signal molecules, macrophages, neutrophils, natural killer cells, complement cascade, T cells or B cells in PubMed and the Web of Science to search for relevant literatures. Conclusion Comprehension of the immune mechanisms ...
Background Denervation is an inevitable pathological situation of renal graft. This study was to ... more Background Denervation is an inevitable pathological situation of renal graft. This study was to explore the change of clock gene rhythm under renal denervation (RDN) and its effect on renal function and oxidative stress during renal ischemia-reperfusion (IR) injury. Method C57/BL6 mice were randomly divided into 4 groups at daytime 7AM (zeitgeber time [ZT] 0) or at nighttime 7PM (ZT12) in respectively: Sham (S) group, RDN group, IR group and RDN+ IR (DIR) group. Renal pathological and functional changes were assessed by H&E staining, and serum creatinine, urea nitrogen and neutrophil gelatinase-associated lipocalin levels. Renal oxidative stress was detected by SOD and MDA levels, and renal inflammation was measured by IL-6, IL-17AF and TNF-ɑ levels. BMAL1, CLOCK, Nrf2 and HO-1 mRNA and protein expressions were tested by qPCR and Western Blot. Result Compared with S groups, the rhythm of BMAL1 and CLOCK genes in the kidney was disordered in RDN groups, while renal pathological and ...
Well-preserved coprolites (fossil faecal pellets) were found from lignite seams of the Lower Cret... more Well-preserved coprolites (fossil faecal pellets) were found from lignite seams of the Lower Cretaceous Huolinhe Formation at the Huolinhe Basin in eastern Inner Mongolia, Northeast China. These coprolites provide a combination of following features: oval to cylindrical shaped with six longitudinal ridges, hexagonal to elliptical cross-sections, and one blunt end and the other pointed end. According to these distinct features and their size range, the producers of these coprolites are attributed to termites. Termites were estimated to have originated in the earliest Cretaceous with an evolutionary radiation in the Early Cretaceous. The presence of wood debris in the coprolites indicate that the Early Cretaceous termites from the Huolinhe Basin had wood-feeding habits; and anatomical features displaying on the wood debris further suggest their feeding preference was coniferous wood. Besides, the results of a k-means clustering analysis performed for these coprolites indicate that thr...
Journal of Materials Science: Materials in Electronics, 2022
In this study, the effects of reflow temperature, reflow time, and substrates (polycrystalline an... more In this study, the effects of reflow temperature, reflow time, and substrates (polycrystalline and (001/110/111) monocrystalline Cu substrates) on the growth of Cu3Sn at the Cu/Sn3Ag interface were investigated. Results show that the growth of Cu3Sn on the interface shows grain boundary diffusion regardless of the orientation of Cu substrates. The formation of Cu3Sn is considered as the reaction between Cu atoms and Cu6Sn5 at Cu6Sn5/Cu3Sn interface due to the similar interfacial outline to Cu substrate. In addition, the growth of interfacial Cu3Sn is positively correlated with reflow temperature and time. And the preferred plane orientation of Cu substrate has a significant effect on the growth rate of Cu3Sn during reflowing. In this experiment, the growth of Cu3Sn shows grain boundary diffusion. The growth of Cu3Sn on polycrystalline Cu substrate is faster because it has a lot grain boundaries. And the growth rate on (110)Cu single crystals is the fastest among single-crystal Cu substrates because the high surface free energy of single-crystal oriented (110)Cu substrates promotes the growth of Cu3Sn and the diffusion distance of Cu atoms is the shortest in this direction.
Objective Hepatic artery thrombosis(HAT) is one of serious complications after liver transplantat... more Objective Hepatic artery thrombosis(HAT) is one of serious complications after liver transplantation. This study was intended to explore the effect of HAT on survival rate of patients/grafts and biliary complications early after liver transplantation with controlled donation after circulatory death (CDCD) donors in children. Methods The clinical data of 236 children with CDCD donor liver transplantation (donor age <14 years) were retrospectively analyzed. Among them, 37 patients developed HAT early postoperatively. The survival rate of patients/grafts and the occurrence of biliary complications were compared between two groups. Results The median follow-up period was 23 months. For HAT (n=37) and non-HAT (n=199) group, the median follow-up period was 27 and 22 months respectively. The 1-year and 3-year survival rates of grafts were 88.2%, 88.2% and 93.2%, 92.4% respectively. And no inter-group statistical difference existed (P=0.373). The 1-year and 3-year graft survival rates we...
Objective To establish a stable model of reduced-size liver transplantations with rejection in ra... more Objective To establish a stable model of reduced-size liver transplantations with rejection in rats. Methods Inbred adult male Lewis rats (weighing 220-250 g, 8-10 weeks old) served as donors in liver transplantation, and inbred adult male Brown Norway (BN) rats (weighing 220-250 g, 8-10 weeks old) served as recipients. Orthotopic group, 50% reduced-size liver transplantation rejection group (50% group) and 30% reduced-size liver transplantation rejection group (30% group) were developed. The 1-, 3-, 7- and 14-survival rate was observed. Besides, the levels of ALT, AST and TBIL in the peripheral blood plasma were determined. The liver tissues were obtained, and HE staining was used to examine the pathological changes and rejection severity of the liver specimens. Results No significant difference in the operation-related indicators was found among the three groups. The 1-, 3-, 7- and 14-day survival rate in orthotopic group was as follows: 91.20%, 86.50%, 81.10% and 75.70%; 85.00%, ...
2020 21st International Conference on Electronic Packaging Technology (ICEPT), 2020
The intermetallic compounds between copper and Sn-based alloy, Cu6Sn5, has a very close relations... more The intermetallic compounds between copper and Sn-based alloy, Cu6Sn5, has a very close relationship with the reliability of solder joints. Due to the trend of miniaturization of electronic products, packaging form began to change from 2D to 3D in the electronics industry. What’ more, The size of the solder joint continues to shrink even to the micron level. As we all know, the total Cu atom and temperature of the center and edge of the spherical joint is different. This difference becomes even greater when the size of the solder joints continues to decrease. In this article, the microstructures of Cu6Sn5 formed at Sn-0.5Cu/(001)Cu interface were investigated. The deviations of morphology, average grain size, and length-width ratio among the center, transition, and edge area revealed that the edge effect is quite significant in Sn-0.5Cu/(001)Cu solder joint during the cooling stage. Therefore, finite element simulation was employed to obtain the distribution of copper concentration ...
Objective To investigate the feasibility and safety of liver graft from little-month-old donors i... more Objective To investigate the feasibility and safety of liver graft from little-month-old donors in pediatric liver transplantation. Methods Clinical data of 101 donors and recipients undergoing liver transplantation from January 2014 to December 2016 in Tianjin First Central Hospital were retrospectively analyzed. 46 recipients were male and 55 female, aged 5.8-40.5 months with a median age of 9.0 months. According to the age of donors, all recipients were divided into the extremely little-month-old group (n=26), little-month-old group (n=22) and normal-month-old group (n=53). The informed consents of all parents of donors were obtained and the local ethical committee approval was received. The perioperative conditions, postoperative complications and survival rate of grafts and recipients were statistically compared and analyzed. The rate comparison was conducted by Chi-square test or Fisher's exact probability test. The survival analysis was performed by Log-rank test. Results...
Objective To investigate and analyze the applications of liver grafts from donation after death c... more Objective To investigate and analyze the applications of liver grafts from donation after death children (≤5 kg) in pediatric liver transplantation. Methods A retrospective analysis was conducted on 18 cases of pediatric recipients using liver grafts from donation after death children (≤5 kg) during January 2013 to December 2015.The survival rate of patients, recovery of liver function, incidence of lung infection, vascular complications and biliary complications were observed, and the causes of deaths were analyzed. Results The median follow-up time of all the patients was 28.4 months (0.06-52.2 months). The 1- and 3-year cumulative survival rate was the same 88.9%, the incidence of lung infection was 22.2%, of vessel complications was 33.3%, and biliary complication rate was 11.1%.Two cases died after liver transplantation. Conclusion The applications of liver donation after death children (≤5 kg) were satisfactory, but there is still relatively limited experience, and further obs...
Objective To evaluate the feasibility and safety of using pediatric donation after brain death do... more Objective To evaluate the feasibility and safety of using pediatric donation after brain death donors during split liver transplantation. Methods The clinical data were retrospectively reviewed for 8 pediatric recipients undergoing split liver transplantation with a donor age of 2.7-7 years. The clinical characteristics of donors/recipients, perioperative course, postoperative recovery and complications along with graft and recipient survival rate were analyzed. Results The split procedure was performed ex situ (n=3) and in situ (n=1), all liver grafts were split into left lateral lobes and extended right lobes. The recipients were children aged 4.7-105.5 months. The mean follow-up period was (8.1±0.6) months and the graft/recipient survival rates approached 100%. Graft functions remained normal in all recipients at the end of follow-ups. Two recipients undergoing liver grafting with long cold ischemia time exhibited slower recovery of graft function. Pathological examination of gra...
Objective: To explore the feasibility of intra-operative cholangiography via the division line of... more Objective: To explore the feasibility of intra-operative cholangiography via the division line of the IV segment bile duct in gallbladder-sparing left lateral hepatic lobectomy for pediatric living-donor liver transplantation. Methods: To perform the gallbladder-sparing left lateral hepatic lobectomy in pediatric living-donor liver transplantation, the liver parenchyma was dissected in the right of falciform ligament, the relative thick IV segment bile duct was dissected and cut off, and the real-time cholangiography was performed through intubation via the proximal bile duct division line. From April 2012 to December 2013, 52 cases of donor were implemented left lateral hepatic lobectomy with this method. According to the result of chloangiography, the type of bile duct and bile duct division line were determined to observe the changes of the donor maximum gallbladder emptying rate. Results: 52 cases of donor intraoperative cholangiography images were clear, with no operation compl...
Background Ischemia and reperfusion injury (IRI) is an ineluctable immune-related pathophysiologi... more Background Ischemia and reperfusion injury (IRI) is an ineluctable immune-related pathophysiological process during organ transplantation, which not only causes a shortage of donor organs, but also has long-term and short-term negative consequences on patients. Severe IRI-induced cell death leads to the release of endogenous substances, which bind specifically to receptors on immune cells to initiate an immune response. Although innate and adaptive immunity have been discovered to play essential roles in IRI in the context of organ transplantation, the pathway and precise involvement of the immune response at various stages has not yet to be elucidated. Methods We combined “IRI” and “organ transplantation” with keywords, respectively such as immune cells, danger signal molecules, macrophages, neutrophils, natural killer cells, complement cascade, T cells or B cells in PubMed and the Web of Science to search for relevant literatures. Conclusion Comprehension of the immune mechanisms ...
Background Denervation is an inevitable pathological situation of renal graft. This study was to ... more Background Denervation is an inevitable pathological situation of renal graft. This study was to explore the change of clock gene rhythm under renal denervation (RDN) and its effect on renal function and oxidative stress during renal ischemia-reperfusion (IR) injury. Method C57/BL6 mice were randomly divided into 4 groups at daytime 7AM (zeitgeber time [ZT] 0) or at nighttime 7PM (ZT12) in respectively: Sham (S) group, RDN group, IR group and RDN+ IR (DIR) group. Renal pathological and functional changes were assessed by H&E staining, and serum creatinine, urea nitrogen and neutrophil gelatinase-associated lipocalin levels. Renal oxidative stress was detected by SOD and MDA levels, and renal inflammation was measured by IL-6, IL-17AF and TNF-ɑ levels. BMAL1, CLOCK, Nrf2 and HO-1 mRNA and protein expressions were tested by qPCR and Western Blot. Result Compared with S groups, the rhythm of BMAL1 and CLOCK genes in the kidney was disordered in RDN groups, while renal pathological and ...
Well-preserved coprolites (fossil faecal pellets) were found from lignite seams of the Lower Cret... more Well-preserved coprolites (fossil faecal pellets) were found from lignite seams of the Lower Cretaceous Huolinhe Formation at the Huolinhe Basin in eastern Inner Mongolia, Northeast China. These coprolites provide a combination of following features: oval to cylindrical shaped with six longitudinal ridges, hexagonal to elliptical cross-sections, and one blunt end and the other pointed end. According to these distinct features and their size range, the producers of these coprolites are attributed to termites. Termites were estimated to have originated in the earliest Cretaceous with an evolutionary radiation in the Early Cretaceous. The presence of wood debris in the coprolites indicate that the Early Cretaceous termites from the Huolinhe Basin had wood-feeding habits; and anatomical features displaying on the wood debris further suggest their feeding preference was coniferous wood. Besides, the results of a k-means clustering analysis performed for these coprolites indicate that thr...
Journal of Materials Science: Materials in Electronics, 2022
In this study, the effects of reflow temperature, reflow time, and substrates (polycrystalline an... more In this study, the effects of reflow temperature, reflow time, and substrates (polycrystalline and (001/110/111) monocrystalline Cu substrates) on the growth of Cu3Sn at the Cu/Sn3Ag interface were investigated. Results show that the growth of Cu3Sn on the interface shows grain boundary diffusion regardless of the orientation of Cu substrates. The formation of Cu3Sn is considered as the reaction between Cu atoms and Cu6Sn5 at Cu6Sn5/Cu3Sn interface due to the similar interfacial outline to Cu substrate. In addition, the growth of interfacial Cu3Sn is positively correlated with reflow temperature and time. And the preferred plane orientation of Cu substrate has a significant effect on the growth rate of Cu3Sn during reflowing. In this experiment, the growth of Cu3Sn shows grain boundary diffusion. The growth of Cu3Sn on polycrystalline Cu substrate is faster because it has a lot grain boundaries. And the growth rate on (110)Cu single crystals is the fastest among single-crystal Cu substrates because the high surface free energy of single-crystal oriented (110)Cu substrates promotes the growth of Cu3Sn and the diffusion distance of Cu atoms is the shortest in this direction.
Objective Hepatic artery thrombosis(HAT) is one of serious complications after liver transplantat... more Objective Hepatic artery thrombosis(HAT) is one of serious complications after liver transplantation. This study was intended to explore the effect of HAT on survival rate of patients/grafts and biliary complications early after liver transplantation with controlled donation after circulatory death (CDCD) donors in children. Methods The clinical data of 236 children with CDCD donor liver transplantation (donor age <14 years) were retrospectively analyzed. Among them, 37 patients developed HAT early postoperatively. The survival rate of patients/grafts and the occurrence of biliary complications were compared between two groups. Results The median follow-up period was 23 months. For HAT (n=37) and non-HAT (n=199) group, the median follow-up period was 27 and 22 months respectively. The 1-year and 3-year survival rates of grafts were 88.2%, 88.2% and 93.2%, 92.4% respectively. And no inter-group statistical difference existed (P=0.373). The 1-year and 3-year graft survival rates we...
Objective To establish a stable model of reduced-size liver transplantations with rejection in ra... more Objective To establish a stable model of reduced-size liver transplantations with rejection in rats. Methods Inbred adult male Lewis rats (weighing 220-250 g, 8-10 weeks old) served as donors in liver transplantation, and inbred adult male Brown Norway (BN) rats (weighing 220-250 g, 8-10 weeks old) served as recipients. Orthotopic group, 50% reduced-size liver transplantation rejection group (50% group) and 30% reduced-size liver transplantation rejection group (30% group) were developed. The 1-, 3-, 7- and 14-survival rate was observed. Besides, the levels of ALT, AST and TBIL in the peripheral blood plasma were determined. The liver tissues were obtained, and HE staining was used to examine the pathological changes and rejection severity of the liver specimens. Results No significant difference in the operation-related indicators was found among the three groups. The 1-, 3-, 7- and 14-day survival rate in orthotopic group was as follows: 91.20%, 86.50%, 81.10% and 75.70%; 85.00%, ...
2020 21st International Conference on Electronic Packaging Technology (ICEPT), 2020
The intermetallic compounds between copper and Sn-based alloy, Cu6Sn5, has a very close relations... more The intermetallic compounds between copper and Sn-based alloy, Cu6Sn5, has a very close relationship with the reliability of solder joints. Due to the trend of miniaturization of electronic products, packaging form began to change from 2D to 3D in the electronics industry. What’ more, The size of the solder joint continues to shrink even to the micron level. As we all know, the total Cu atom and temperature of the center and edge of the spherical joint is different. This difference becomes even greater when the size of the solder joints continues to decrease. In this article, the microstructures of Cu6Sn5 formed at Sn-0.5Cu/(001)Cu interface were investigated. The deviations of morphology, average grain size, and length-width ratio among the center, transition, and edge area revealed that the edge effect is quite significant in Sn-0.5Cu/(001)Cu solder joint during the cooling stage. Therefore, finite element simulation was employed to obtain the distribution of copper concentration ...
Objective To investigate the feasibility and safety of liver graft from little-month-old donors i... more Objective To investigate the feasibility and safety of liver graft from little-month-old donors in pediatric liver transplantation. Methods Clinical data of 101 donors and recipients undergoing liver transplantation from January 2014 to December 2016 in Tianjin First Central Hospital were retrospectively analyzed. 46 recipients were male and 55 female, aged 5.8-40.5 months with a median age of 9.0 months. According to the age of donors, all recipients were divided into the extremely little-month-old group (n=26), little-month-old group (n=22) and normal-month-old group (n=53). The informed consents of all parents of donors were obtained and the local ethical committee approval was received. The perioperative conditions, postoperative complications and survival rate of grafts and recipients were statistically compared and analyzed. The rate comparison was conducted by Chi-square test or Fisher's exact probability test. The survival analysis was performed by Log-rank test. Results...
Objective To investigate and analyze the applications of liver grafts from donation after death c... more Objective To investigate and analyze the applications of liver grafts from donation after death children (≤5 kg) in pediatric liver transplantation. Methods A retrospective analysis was conducted on 18 cases of pediatric recipients using liver grafts from donation after death children (≤5 kg) during January 2013 to December 2015.The survival rate of patients, recovery of liver function, incidence of lung infection, vascular complications and biliary complications were observed, and the causes of deaths were analyzed. Results The median follow-up time of all the patients was 28.4 months (0.06-52.2 months). The 1- and 3-year cumulative survival rate was the same 88.9%, the incidence of lung infection was 22.2%, of vessel complications was 33.3%, and biliary complication rate was 11.1%.Two cases died after liver transplantation. Conclusion The applications of liver donation after death children (≤5 kg) were satisfactory, but there is still relatively limited experience, and further obs...
Objective To evaluate the feasibility and safety of using pediatric donation after brain death do... more Objective To evaluate the feasibility and safety of using pediatric donation after brain death donors during split liver transplantation. Methods The clinical data were retrospectively reviewed for 8 pediatric recipients undergoing split liver transplantation with a donor age of 2.7-7 years. The clinical characteristics of donors/recipients, perioperative course, postoperative recovery and complications along with graft and recipient survival rate were analyzed. Results The split procedure was performed ex situ (n=3) and in situ (n=1), all liver grafts were split into left lateral lobes and extended right lobes. The recipients were children aged 4.7-105.5 months. The mean follow-up period was (8.1±0.6) months and the graft/recipient survival rates approached 100%. Graft functions remained normal in all recipients at the end of follow-ups. Two recipients undergoing liver grafting with long cold ischemia time exhibited slower recovery of graft function. Pathological examination of gra...
Uploads
Papers by Chong Dong