Diabetes mellitus is a metabolic disease that has posed a public health problem for a long time a... more Diabetes mellitus is a metabolic disease that has posed a public health problem for a long time and whose medical treatment is lifelong and expensive. Thus, populations have the alternative treatment of using medicinal plants. The aim of this work was to evaluate the antidiabetic activity of the aqueous extract of the trunk bark of Sclerocarya birrea (EAqScB) and its cyclohexane fraction (F4) in order to justify its traditional use in the treatment of diabetes mellitus. The study was done on rats induced type 2 diabetic by injection of streptozotocin (STZ). These animals are administered a daily dose of EAqScB and F4 for 28 days.The rats blood sugar was measured regularly every 7 days. At the end of the experiment, the rats are fasted and anesthetized, then the blood of these animals is taken from the orbital sinus using a Pasteur pipette, in order to determine their hematological and serum parameters. EAqScB and F4 at the respective doses of 100 and 25 mg/kg B.W. induced a very sig...
Evidence-Based Complementary and Alternative Medicine, 2019
Native to Mexico, Persea americana Mill. (avocado) is a fruit tree whose different parts (leaf, b... more Native to Mexico, Persea americana Mill. (avocado) is a fruit tree whose different parts (leaf, bark, roots, and stone) are used in traditional medicine especially against diabetes mellitus. The aim of this study was to investigate the beneficial effects of 28-day treatment with aqueous, ethanolic, and methanolic leaf extracts on glucose homeostasis in type 2 diabetic mellitus using Wistar rats. Type 2 diabetes was induced with nicotinamide (120 mg/kg, i.p.) and streptozotocin (65 mg/kg, i.p.). After 28 days of treatment, histopathological examination of the pancreas, kidneys, liver, and muscle (tibialis anterior) were realized. Biochemical markers were determined and an intestinal absorption test of D-glucose was performed. All extracts (100 mg/kg/day, p.o.) significantly (p<0.001) reduced blood glucose level at the 28th day of treatment with a more pronounced effect for methanolic extract. The treatments were well tolerated and induced a restoration of T-CHOL and HDL-C levels c...
Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to... more Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to treat many diseases including hypertension. This study investigated whether oral administration of the ethyl acetate fraction of Lannea microcarpa trunk barks (LMAE) corrects angiotensin (Ang) II-induced hypertension in mice. Its effects on vascular function were specifically investigated. Experiments explored hemodynamic and echocardiographic parameters in vivo and vascular reactivity to acetylcholine (ACh) and CaCl2ex vivo on isolated aortas. Mice received LMAE for 3 weeks (50 mg/kg/day) by oral gavage. In the last two weeks of treatment, mice were implanted with osmotic minipumps delivering NaCl (0.9%) or Ang II (0.5 mg/kg/day). LMAE completely prevented the increase in systolic and diastolic blood pressure induced by Ang II. Echocardiographic and kidney parameters were not affected by the different conditions. LMAE abrogated Ang II-induced impairment of ACh-induced relaxation withou...
Despite long traditional utilization and some reports on the antihyperglycemic and antihyperlipid... more Despite long traditional utilization and some reports on the antihyperglycemic and antihyperlipidemic action ofCassia siamea, the mechanisms involved have not been investigated yet. Thus, the objective of the present study was to investigate whether and how oral administration of the ethanolic extract ofCassia siamea Lamleaves (LECS) improves glucose and insulin homoeostasis, liver damage, and endothelial dysfunction in an experimental model of type 2 diabetes, the leptin-deficientob/obmice. Oxidative stress and protein expression of insulin-dependent and insulin -independent signaling pathways were studied. Obese(ob/ob) vs. control (ob/+) mice were treated daily with intragastric administration of either vehicle or LECS (200 mg/kg, per day) for 4 weeks. Fasting blood glucose, body weight, food intake, glucose and insulin tolerance, oxidative stress, and liver damage as well as vascular complications with respect to endothelial dysfunction were examined. Administration of LECS in ob...
Obstructive sleep apnea is characterized by intermittent hypoxia (IH) which alters endothelial fu... more Obstructive sleep apnea is characterized by intermittent hypoxia (IH) which alters endothelial function, induces inflammation and accelerates atherosclerosis-induced cardiovascular diseases. The non-muscular myosin light chain kinase (nmMLCK) isoform contributes to endothelial cell-cell junction opening. Deletion of nmMLCK protects mice from death in septic shock models and prevents atherosclerosis in high-fat diet-fed mice. The aim of the study was to analyze the implication of nmMLCK in IH-induced vascular inflammation. Human aortic endothelial cells were exposed to 6 hours of IH in absence or presence of nmMLCK inhibitors, ML-7 (5 µM) or PIK (150 µM). IH increased reactive oxygen species (ROS) and nitric oxide (NO) production, p65-NFκB activation and IL-6 secretion. While nmMLCK inhibition did not prevent IH-induced ROS production and p65-NFκB activation, it decreased NO production and partially prevented IL-6 secretion. IH-induced IL-6 secretion and vesicle-associated membrane p...
Diabetes mellitus is a metabolic disease that has posed a public health problem for a long time a... more Diabetes mellitus is a metabolic disease that has posed a public health problem for a long time and whose medical treatment is lifelong and expensive. Thus, populations have the alternative treatment of using medicinal plants. The aim of this work was to evaluate the antidiabetic activity of the aqueous extract of the trunk bark of Sclerocarya birrea (EAqScB) and its cyclohexane fraction (F4) in order to justify its traditional use in the treatment of diabetes mellitus. The study was done on rats induced type 2 diabetic by injection of streptozotocin (STZ). These animals are administered a daily dose of EAqScB and F4 for 28 days.The rats blood sugar was measured regularly every 7 days. At the end of the experiment, the rats are fasted and anesthetized, then the blood of these animals is taken from the orbital sinus using a Pasteur pipette, in order to determine their hematological and serum parameters. EAqScB and F4 at the respective doses of 100 and 25 mg/kg B.W. induced a very sig...
Evidence-Based Complementary and Alternative Medicine, 2019
Native to Mexico, Persea americana Mill. (avocado) is a fruit tree whose different parts (leaf, b... more Native to Mexico, Persea americana Mill. (avocado) is a fruit tree whose different parts (leaf, bark, roots, and stone) are used in traditional medicine especially against diabetes mellitus. The aim of this study was to investigate the beneficial effects of 28-day treatment with aqueous, ethanolic, and methanolic leaf extracts on glucose homeostasis in type 2 diabetic mellitus using Wistar rats. Type 2 diabetes was induced with nicotinamide (120 mg/kg, i.p.) and streptozotocin (65 mg/kg, i.p.). After 28 days of treatment, histopathological examination of the pancreas, kidneys, liver, and muscle (tibialis anterior) were realized. Biochemical markers were determined and an intestinal absorption test of D-glucose was performed. All extracts (100 mg/kg/day, p.o.) significantly (p<0.001) reduced blood glucose level at the 28th day of treatment with a more pronounced effect for methanolic extract. The treatments were well tolerated and induced a restoration of T-CHOL and HDL-C levels c...
Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to... more Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to treat many diseases including hypertension. This study investigated whether oral administration of the ethyl acetate fraction of Lannea microcarpa trunk barks (LMAE) corrects angiotensin (Ang) II-induced hypertension in mice. Its effects on vascular function were specifically investigated. Experiments explored hemodynamic and echocardiographic parameters in vivo and vascular reactivity to acetylcholine (ACh) and CaCl2ex vivo on isolated aortas. Mice received LMAE for 3 weeks (50 mg/kg/day) by oral gavage. In the last two weeks of treatment, mice were implanted with osmotic minipumps delivering NaCl (0.9%) or Ang II (0.5 mg/kg/day). LMAE completely prevented the increase in systolic and diastolic blood pressure induced by Ang II. Echocardiographic and kidney parameters were not affected by the different conditions. LMAE abrogated Ang II-induced impairment of ACh-induced relaxation withou...
Despite long traditional utilization and some reports on the antihyperglycemic and antihyperlipid... more Despite long traditional utilization and some reports on the antihyperglycemic and antihyperlipidemic action ofCassia siamea, the mechanisms involved have not been investigated yet. Thus, the objective of the present study was to investigate whether and how oral administration of the ethanolic extract ofCassia siamea Lamleaves (LECS) improves glucose and insulin homoeostasis, liver damage, and endothelial dysfunction in an experimental model of type 2 diabetes, the leptin-deficientob/obmice. Oxidative stress and protein expression of insulin-dependent and insulin -independent signaling pathways were studied. Obese(ob/ob) vs. control (ob/+) mice were treated daily with intragastric administration of either vehicle or LECS (200 mg/kg, per day) for 4 weeks. Fasting blood glucose, body weight, food intake, glucose and insulin tolerance, oxidative stress, and liver damage as well as vascular complications with respect to endothelial dysfunction were examined. Administration of LECS in ob...
Obstructive sleep apnea is characterized by intermittent hypoxia (IH) which alters endothelial fu... more Obstructive sleep apnea is characterized by intermittent hypoxia (IH) which alters endothelial function, induces inflammation and accelerates atherosclerosis-induced cardiovascular diseases. The non-muscular myosin light chain kinase (nmMLCK) isoform contributes to endothelial cell-cell junction opening. Deletion of nmMLCK protects mice from death in septic shock models and prevents atherosclerosis in high-fat diet-fed mice. The aim of the study was to analyze the implication of nmMLCK in IH-induced vascular inflammation. Human aortic endothelial cells were exposed to 6 hours of IH in absence or presence of nmMLCK inhibitors, ML-7 (5 µM) or PIK (150 µM). IH increased reactive oxygen species (ROS) and nitric oxide (NO) production, p65-NFκB activation and IL-6 secretion. While nmMLCK inhibition did not prevent IH-induced ROS production and p65-NFκB activation, it decreased NO production and partially prevented IL-6 secretion. IH-induced IL-6 secretion and vesicle-associated membrane p...
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Papers by Camille KOFFI