Papers by Borys Błaszczak
Alergia
Spongiform encephalopthies are the human and other mammal diseases caused by proins - infectious ... more Spongiform encephalopthies are the human and other mammal diseases caused by proins - infectious protein particles. Diseases are either transmissible or sporadic. It is believed that prions /PrPsc / are altered form of the normal cellular protein /PrPc / coded by prnp gene in human and animal cells. Normal PrPc has a- helix conformation, which changes to b - sheet form as a consequence of the interaction with the infectious PrPsc. It occurs when host organism is invaded by prions through the oral or jatrogenic route. The spontaneous conversion from PrPc to PrPsc may occurs in human whose PRNP gene carries mutations in certain codons. The molecular mechanism of the conversion into infectious form is not fully understood, especially in case of spontaneous form of disease. There are few hypotheses that attempt to explain the origin of PrPsc appearance what is the issue of the article. Przed blisko dwudziesty laty Stanley Prusiner /laureat nagrody Nobla z 1997 roku/ wywołał znaczne zami...
The Scientific World Journal, 2015
The antimicrobial susceptibility of Escherichia coli isolates associated with various types of in... more The antimicrobial susceptibility of Escherichia coli isolates associated with various types of infections in dogs and cats was determined. The studied isolates were most frequently susceptible to fluoroquinolones and the extended-spectrum cephalosporins (ESCs), antimicrobials commonly used in treatment of infections in companion animals. However, an increase in the percentage of strains resistant to -lactam antibiotics including ESCs was noted between January 2007 and December 2013. The frequency of multidrug-resistant (MDR) E. coli isolation (66.8% of isolates) is alarming. Moreover, the statistically significant increase of the percentage of MDR isolates was observed during the study period. No difference in the prevalence of multidrug resistance was found between bacteria causing intestinal and extraintestinal infections and between canine and feline isolates. Nonhemolytic E. coli isolates were MDR more often than hemolytic ones. Our study showed the companion animals in Poland as an important reservoir of MDR bacteria. These results indicate that continuous monitoring of canine and feline E. coli antimicrobial susceptibility is required. Furthermore, introduction and application of recommendations for appropriate use of antimicrobials in small animal practice should be essential to minimize the emergence of multidrug resistance among E. coli in companion animals.
ABSTRACT Physiological properties and proportion of typical features of Pityrosporum pachydermati... more ABSTRACT Physiological properties and proportion of typical features of Pityrosporum pachydermatis were determined on 385 strains from clinical cases of O.E and dermatitis in dogs. Carbohydrates and nitrogen assimilation were determined auxanographically. Urease production and enzyme release were assessed on Christensen's medium and API-ZYM respectively. All strains oxidised carbohydrates in the OF test. 90% assimilation of glucose and production of urease are typical of Pityrosporum, contrary to 100% positive reactions in literature data. Production of acid and alkaline phosphatases, phosphohydrolase, leucin arylamidase, and beta-glucosidase dominated, while lipase C14, esterase-lipase C8, esterase C14 and alpha-galactosidase were variable.
Medycyna weterynaryjna
The plasmid-encoded spv virulence genes common to the Salmonella enetrica subspecies 1 have been ... more The plasmid-encoded spv virulence genes common to the Salmonella enetrica subspecies 1 have been correlated lvith systemic salmonellosis. The spv region consists of spvABCD, which are transcribed as an operon. They are found in a broad spectrum of serowars of Salmonella adapted to different animal species. The exact mechanism by which the spv genes increase the growth rate within host macrophages is not known. The present study was undertaken to determine the frequency of plasmid-encoded spv gene occurrence among S. Enteritidis strains isolated from poultry. The study involved 70 strains of S. Enteritidis. SpvR and/or spvD genes detected by PCR method were found in about 807o of the tested strains. Both spvR and spvD genes wer€ present in 54oń of the isolates. About 21oń of S. Enteritidis demonstrated only spvR and l2.5oń only spvD genes. The mo|ecular size of isolated Salmonella plasmids were compared to those from Y. enterocolitica.
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Papers by Borys Błaszczak