The asymptomatic nature, high rate of disease recurrence, and resistance to platinum-based chemot... more The asymptomatic nature, high rate of disease recurrence, and resistance to platinum-based chemotherapy highlight the need to identify and characterize novel target molecules for ovarian cancer. Fibroblast growth factor 8 (FGF8) aids in the development and metastasis of ovarian cancer; however, its definite role is not clear. We employed ELISA and IHC to examine the expression of FGF8 in the saliva and tissue samples of epithelial ovarian cancer (EOC) patients and controls. Furthermore, various cell assays were conducted to determine how FGF8 silencing influences ovarian cancer cell survival, adhesion, migration, and invasion to learn more about the functions of FGF8. In saliva samples, from controls through low-grade to high-grade EOC, a stepped overexpression of FGF8 was observed. Similar expression trends were seen in tissue samples, both at protein and mRNA levels. FGF8 gene silencing in SKOV3 cells adversely affected various cell properties essential for cancer cell survival an...
Journal of Reproductive Healthcare and Medicine, 2021
Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer”... more Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer” which is being considered as most lethal gynecological cancer by acquiring the fifth leading cause of cancer-related deaths in women. Detection of ovarian cancer frequently requires painful invasive procedures such as multiple biopsies and blood tests which results in an undue stress and discomforts in patient. Recently, saliva is being opted as an alternative source for biomarker discovery due to procedure being non-invasive collection method cost-effectiveness, ease of sample collection, and handling. Saliva, a multiconstituent oral fluid secretion from the major and minor salivary glands enriched with proteins, DNAs, RNAs, and metabolites, behaves as a “mirror of the body.” Salivary diagnostics has become booming field with the development of new and advanced technologies accompanying with proteomics, RNA sequencing, liquid biopsy, and point-of-care (POC) diagnostics. Reliable and re...
Ovarian cancer, the most lethal gynecological cancer, is the fifth most common cause of cancer-re... more Ovarian cancer, the most lethal gynecological cancer, is the fifth most common cause of cancer-related deaths in women. A cost-effective and non-invasive early screening method for ovarian cancer is required to reduce the high mortality rate. Saliva is a clinically informative unique fluid, which is useful for novel approaches to prognosis, clinical diagnosis, and monitoring for non-invasive detection of disease. Multimerin1 (MMRN1) is a di-sulfide linked homo-polymeric glycoprotein from EMILIN family. Altered expression of MMRN1 has been reported in hepatocellular carcinoma, cervical cancer, and ovarian cancer. But, its role in epithelial ovarian cancer (EOC) is not clear and well documented. In this study, expression of Multimerin 1 was validated in saliva and tissues of EOC patients and age-matched controls by western blotting, ELISA, RT-PCR, and immunohistochemistry. Significant over expression of MMRN1 was observed by western blot and ELISA in saliva samples of EOC patients. The average concentration of MMRN1 in the saliva of healthy controls was 28.7 pg/ml (SE ± 1.76), 42.53 pg/ml (SE ± 4.06) in low grade and 52.91 pg/ml (SE ± 4.24) with p < 0.01 in high-grade EOC. Upregulated cytoplasmic expression of MMRN1 was observed in EOC tissue by immunohistochemistry. Our results suggest that MMRN1 expression is associated with EOC progression and MMRN1 may be potential biomarker candidates for early-stage EOC detection however further experiments are required in a large cohort to establish this proposition. Also, saliva can be explored as a novel medium for ovarian cancer diagnosis.
Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer”... more Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer” which is being considered as most lethal gynecological cancer by acquiring the fifth leading cause of cancer-related deaths in women. Detection of ovarian cancer frequently requires painful invasive procedures such as multiple biopsies and blood tests which results in an undue stress and discomforts in patient. Recently, saliva is being opted as an alternative source for biomarker discovery due to procedure being non-invasive collection method cost-effectiveness, ease of sample collection, and handling. Saliva, a multiconstituent oral fluid secretion from the major and minor salivary glands enriched with proteins, DNAs, RNAs, and metabolites, behaves as a “mirror of the body.” Salivary diagnostics has become booming field with the development of new and advanced technologies accompanying with proteomics, RNA sequencing, liquid biopsy, and point-of-care (POC) diagnostics. Reliable and re...
The asymptomatic nature, high rate of disease recurrence, and resistance to platinum-based chemot... more The asymptomatic nature, high rate of disease recurrence, and resistance to platinum-based chemotherapy highlight the need to identify and characterize novel target molecules for ovarian cancer. Fibroblast growth factor 8 (FGF8) aids in the development and metastasis of ovarian cancer; however, its definite role is not clear. We employed ELISA and IHC to examine the expression of FGF8 in the saliva and tissue samples of epithelial ovarian cancer (EOC) patients and controls. Furthermore, various cell assays were conducted to determine how FGF8 silencing influences ovarian cancer cell survival, adhesion, migration, and invasion to learn more about the functions of FGF8. In saliva samples, from controls through low-grade to high-grade EOC, a stepped overexpression of FGF8 was observed. Similar expression trends were seen in tissue samples, both at protein and mRNA levels. FGF8 gene silencing in SKOV3 cells adversely affected various cell properties essential for cancer cell survival an...
Journal of Reproductive Healthcare and Medicine, 2021
Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer”... more Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer” which is being considered as most lethal gynecological cancer by acquiring the fifth leading cause of cancer-related deaths in women. Detection of ovarian cancer frequently requires painful invasive procedures such as multiple biopsies and blood tests which results in an undue stress and discomforts in patient. Recently, saliva is being opted as an alternative source for biomarker discovery due to procedure being non-invasive collection method cost-effectiveness, ease of sample collection, and handling. Saliva, a multiconstituent oral fluid secretion from the major and minor salivary glands enriched with proteins, DNAs, RNAs, and metabolites, behaves as a “mirror of the body.” Salivary diagnostics has become booming field with the development of new and advanced technologies accompanying with proteomics, RNA sequencing, liquid biopsy, and point-of-care (POC) diagnostics. Reliable and re...
Ovarian cancer, the most lethal gynecological cancer, is the fifth most common cause of cancer-re... more Ovarian cancer, the most lethal gynecological cancer, is the fifth most common cause of cancer-related deaths in women. A cost-effective and non-invasive early screening method for ovarian cancer is required to reduce the high mortality rate. Saliva is a clinically informative unique fluid, which is useful for novel approaches to prognosis, clinical diagnosis, and monitoring for non-invasive detection of disease. Multimerin1 (MMRN1) is a di-sulfide linked homo-polymeric glycoprotein from EMILIN family. Altered expression of MMRN1 has been reported in hepatocellular carcinoma, cervical cancer, and ovarian cancer. But, its role in epithelial ovarian cancer (EOC) is not clear and well documented. In this study, expression of Multimerin 1 was validated in saliva and tissues of EOC patients and age-matched controls by western blotting, ELISA, RT-PCR, and immunohistochemistry. Significant over expression of MMRN1 was observed by western blot and ELISA in saliva samples of EOC patients. The average concentration of MMRN1 in the saliva of healthy controls was 28.7 pg/ml (SE ± 1.76), 42.53 pg/ml (SE ± 4.06) in low grade and 52.91 pg/ml (SE ± 4.24) with p < 0.01 in high-grade EOC. Upregulated cytoplasmic expression of MMRN1 was observed in EOC tissue by immunohistochemistry. Our results suggest that MMRN1 expression is associated with EOC progression and MMRN1 may be potential biomarker candidates for early-stage EOC detection however further experiments are required in a large cohort to establish this proposition. Also, saliva can be explored as a novel medium for ovarian cancer diagnosis.
Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer”... more Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer” which is being considered as most lethal gynecological cancer by acquiring the fifth leading cause of cancer-related deaths in women. Detection of ovarian cancer frequently requires painful invasive procedures such as multiple biopsies and blood tests which results in an undue stress and discomforts in patient. Recently, saliva is being opted as an alternative source for biomarker discovery due to procedure being non-invasive collection method cost-effectiveness, ease of sample collection, and handling. Saliva, a multiconstituent oral fluid secretion from the major and minor salivary glands enriched with proteins, DNAs, RNAs, and metabolites, behaves as a “mirror of the body.” Salivary diagnostics has become booming field with the development of new and advanced technologies accompanying with proteomics, RNA sequencing, liquid biopsy, and point-of-care (POC) diagnostics. Reliable and re...
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Papers by Binita Chandra