Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the ... more Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the age of five years in both developed and developing countries. Human lactadherin, a milk-fat globule membrane (MFGM) glycoprotein, inhibits human rotavirus infection in vitro, whereas bovine lactadherin is not active. Moreover, it protects breastfed infants against symptomatic rotavirus infections. To explore the potential antiviral activity of lactadherin sourced by equines we undertook a proteomic analysis of MFGM proteins from donkey milk and elucidated its amino acid sequence. Alignment of the human, bovine and donkey lactadherin sequences revealed the presence of an Asp-Gly-Glu (DGE) α2β1 integrin-binding motif in the N-terminal domain of donkey sequence only. Since integrin α2β1 plays a critical role during early steps of rotavirus host cell adhesion, we tested a mini-library of donkey lactadherin-derived peptides containing DGE sequence for anti-rotavirus activity. A 20 amino acid ...
Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the ... more Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the age of five years in both developed and developing countries. Human lactadherin, a milk-fat globule membrane (MFGM) glycoprotein, inhibits human rotavirus infection in vitro, whereas bovine lactadherin is not active. Moreover, it protects breastfed infants against symptomatic rotavirus infections. To explore the potential antiviral activity of lactadherin sourced by equines we undertook a proteomic analysis of MFGM proteins from donkey milk and elucidated its amino acid sequence. Alignment of the human, bovine and donkey lactadherin sequences revealed the presence of an Asp-Gly-Glu (DGE) α2β1 integrin-binding motif in the N-terminal domain of donkey sequence only. Since integrin α2β1 plays a critical role during early steps of rotavirus host cell adhesion, we tested a mini-library of donkey lactadherin-derived peptides containing DGE sequence for anti-rotavirus activity. A 20 amino acid ...
Although several studies have aimed to identify mare&... more Although several studies have aimed to identify mare's milk proteins, only the major whey proteins and some caseins have yet been characterized. Incomplete sequencing of the equine genome and the difficulty of recovering highly hydrophobic proteins mean that little is known to date about the proteins associated with milk fat globules, which have been shown to play an important role in newborns' defense mechanisms. The fat fraction, in particular the distribution of unsaturated fatty acids, has been more extensively studied, but complex lipids are only partially elucidated. This study reports a 2-DE approach combined with a powerful method for de novo protein sequencing, and quali-quantitative data on complex lipid composition determined by high performance TLC (HPTLC) and GC. The presence in mare's milk of long-chain highly unsaturated fatty acids, and the evidence of close similarity between equine and human milk fat globule membrane proteins, support the use of mare's milk for human nutrition.
Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the ... more Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the age of five years in both developed and developing countries. Human lactadherin, a milk-fat globule membrane (MFGM) glycoprotein, inhibits human rotavirus infection in vitro, whereas bovine lactadherin is not active. Moreover, it protects breastfed infants against symptomatic rotavirus infections. To explore the potential antiviral activity of lactadherin sourced by equines we undertook a proteomic analysis of MFGM proteins from donkey milk and elucidated its amino acid sequence. Alignment of the human, bovine and donkey lactadherin sequences revealed the presence of an Asp-Gly-Glu (DGE) α2β1 integrin-binding motif in the N-terminal domain of donkey sequence only. Since integrin α2β1 plays a critical role during early steps of rotavirus host cell adhesion, we tested a mini-library of donkey lactadherin-derived peptides containing DGE sequence for anti-rotavirus activity. A 20 amino acid ...
Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the ... more Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the age of five years in both developed and developing countries. Human lactadherin, a milk-fat globule membrane (MFGM) glycoprotein, inhibits human rotavirus infection in vitro, whereas bovine lactadherin is not active. Moreover, it protects breastfed infants against symptomatic rotavirus infections. To explore the potential antiviral activity of lactadherin sourced by equines we undertook a proteomic analysis of MFGM proteins from donkey milk and elucidated its amino acid sequence. Alignment of the human, bovine and donkey lactadherin sequences revealed the presence of an Asp-Gly-Glu (DGE) α2β1 integrin-binding motif in the N-terminal domain of donkey sequence only. Since integrin α2β1 plays a critical role during early steps of rotavirus host cell adhesion, we tested a mini-library of donkey lactadherin-derived peptides containing DGE sequence for anti-rotavirus activity. A 20 amino acid ...
Although several studies have aimed to identify mare&... more Although several studies have aimed to identify mare's milk proteins, only the major whey proteins and some caseins have yet been characterized. Incomplete sequencing of the equine genome and the difficulty of recovering highly hydrophobic proteins mean that little is known to date about the proteins associated with milk fat globules, which have been shown to play an important role in newborns' defense mechanisms. The fat fraction, in particular the distribution of unsaturated fatty acids, has been more extensively studied, but complex lipids are only partially elucidated. This study reports a 2-DE approach combined with a powerful method for de novo protein sequencing, and quali-quantitative data on complex lipid composition determined by high performance TLC (HPTLC) and GC. The presence in mare's milk of long-chain highly unsaturated fatty acids, and the evidence of close similarity between equine and human milk fat globule membrane proteins, support the use of mare's milk for human nutrition.
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