This text examines the various facets of intellectual property and the science and technology pol... more This text examines the various facets of intellectual property and the science and technology policies necessary for its generation and utilization within specific economies. Contributions come from experts in both NATO and CIS countries.
... In addition deep thanks are due to the Directors of the ARW, Prof. Carlo Corsi and Prof. ... ... more ... In addition deep thanks are due to the Directors of the ARW, Prof. Carlo Corsi and Prof. ... Programme Organization The Organizing Committee of the NATO ARW was headed by: Prof. Carlo Corsi, General Director Consorzio Roma Ricerche, Italy Prof. ...
This article examines the mechanism of salinity resistance of varieties and the biochemical prope... more This article examines the mechanism of salinity resistance of varieties and the biochemical properties of the interaction of phytohormones with the antioxidant system by studying the exogenous effects of abscisic and indole-acetic acids on the antioxidant system of Gulistan and Porloq-1 cotton varieties under salinity conditions (1% and 4% NaCl)
Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues incl... more Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (-)-enantiomer is more toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (-)-gossypol requires determination of the (+)- to (-)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in cotton tissues using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50μm, effective length of 24.5cm, 15kV and cassette temperature of 15°C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36ng/mL providing base line separation in less than 6min.
Verticillium dahliae Kleb. is a phytopathogenic fungi which causes wilt disease in a wide range o... more Verticillium dahliae Kleb. is a phytopathogenic fungi which causes wilt disease in a wide range of crops, including cotton. In this study, we have examined the role of the peroxidase (POX) enzymes in cotton response to pathogen. We examined the inducibility of POXs through the experimental explosion of cotton (Gossypium hirsutum L.) cultivars by V. dahliae. The results of this study indicate that POX activity was associated with resistance and that its activity was correlated with levels of different resistance of cotton cultivars. POX activity of 7-day-old cotton seedlings increased in the presence of V. dahliae chitin and conidia in comparison with its activity in crude extract. This activation occurred in the chitin-sorbed fraction. Isoelectric focusing shows that cotton seedlings of var. ‘AN-Bayaut-2’ are considered to be resistant and had two anionic isoforms (pI 3.4 and 3.9) that bind to V. dahliae while ‘C-4727’ is considered to be susceptible and had only one of these types ...
We determined the effect of exogenous indole-3-acetic acid, -naphthalene-3-acetic acid and gibber... more We determined the effect of exogenous indole-3-acetic acid, -naphthalene-3-acetic acid and gibberellic acid (GA3) on the enzymatic activity of glucansynthase, peroxidase and cellulase in ovule development of naked L-70 and linted AN-Bayaut-2 cotton (Gossypium hirsutum L.) seeds. We isolated a protein–inhibitor of 37 kDa with pI 4.2 from integument tissue of naked cotton seeds. In addition, we studied its inhibitory activity on the biosynthesis of cellulose after GA3 treatment of ovules of linted cottonseed at 10 M. The results help to illuminate our understanding of lint development in cotton. _____________________________________________________________________________________________________________
Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues incl... more Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (-)-enantiomer is more toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (-)-gossypol requires determination of the (+)- to (-)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in cotton tissues using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50μm, effective length of 24.5cm, 15kV and cassette temperature of 15°C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36ng/mL providing base line separation in less than 6min.
This text examines the various facets of intellectual property and the science and technology pol... more This text examines the various facets of intellectual property and the science and technology policies necessary for its generation and utilization within specific economies. Contributions come from experts in both NATO and CIS countries.
... In addition deep thanks are due to the Directors of the ARW, Prof. Carlo Corsi and Prof. ... ... more ... In addition deep thanks are due to the Directors of the ARW, Prof. Carlo Corsi and Prof. ... Programme Organization The Organizing Committee of the NATO ARW was headed by: Prof. Carlo Corsi, General Director Consorzio Roma Ricerche, Italy Prof. ...
This article examines the mechanism of salinity resistance of varieties and the biochemical prope... more This article examines the mechanism of salinity resistance of varieties and the biochemical properties of the interaction of phytohormones with the antioxidant system by studying the exogenous effects of abscisic and indole-acetic acids on the antioxidant system of Gulistan and Porloq-1 cotton varieties under salinity conditions (1% and 4% NaCl)
Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues incl... more Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (-)-enantiomer is more toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (-)-gossypol requires determination of the (+)- to (-)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in cotton tissues using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50μm, effective length of 24.5cm, 15kV and cassette temperature of 15°C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36ng/mL providing base line separation in less than 6min.
Verticillium dahliae Kleb. is a phytopathogenic fungi which causes wilt disease in a wide range o... more Verticillium dahliae Kleb. is a phytopathogenic fungi which causes wilt disease in a wide range of crops, including cotton. In this study, we have examined the role of the peroxidase (POX) enzymes in cotton response to pathogen. We examined the inducibility of POXs through the experimental explosion of cotton (Gossypium hirsutum L.) cultivars by V. dahliae. The results of this study indicate that POX activity was associated with resistance and that its activity was correlated with levels of different resistance of cotton cultivars. POX activity of 7-day-old cotton seedlings increased in the presence of V. dahliae chitin and conidia in comparison with its activity in crude extract. This activation occurred in the chitin-sorbed fraction. Isoelectric focusing shows that cotton seedlings of var. ‘AN-Bayaut-2’ are considered to be resistant and had two anionic isoforms (pI 3.4 and 3.9) that bind to V. dahliae while ‘C-4727’ is considered to be susceptible and had only one of these types ...
We determined the effect of exogenous indole-3-acetic acid, -naphthalene-3-acetic acid and gibber... more We determined the effect of exogenous indole-3-acetic acid, -naphthalene-3-acetic acid and gibberellic acid (GA3) on the enzymatic activity of glucansynthase, peroxidase and cellulase in ovule development of naked L-70 and linted AN-Bayaut-2 cotton (Gossypium hirsutum L.) seeds. We isolated a protein–inhibitor of 37 kDa with pI 4.2 from integument tissue of naked cotton seeds. In addition, we studied its inhibitory activity on the biosynthesis of cellulose after GA3 treatment of ovules of linted cottonseed at 10 M. The results help to illuminate our understanding of lint development in cotton. _____________________________________________________________________________________________________________
Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues incl... more Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (-)-enantiomer is more toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (-)-gossypol requires determination of the (+)- to (-)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in cotton tissues using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50μm, effective length of 24.5cm, 15kV and cassette temperature of 15°C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36ng/mL providing base line separation in less than 6min.
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