Papers by Alejandra Vargas Camargo
Fil: Martinotti, Marcelo Diego. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Arge... more Fil: Martinotti, Marcelo Diego. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Frontiers in Chemistry, 2021
Among healthy vegetables, those of the genus Allium stand out. Antioxidant and anti-inflammatory ... more Among healthy vegetables, those of the genus Allium stand out. Antioxidant and anti-inflammatory properties have been associated with these vegetables, attributed mainly to organosulfur compounds (OSCs). In turn, they are linked to a protective effect counteracting cardiovascular disease development. Now, to really ensure the bioactive efficacy of the said compounds once consumed, it is necessary to previously evaluate the ADME (absorption, distribution, metabolism, and excretion) profile. Alternatively, in vitro and in silico methods attempt to avoid or reduce experimental animals’ use and provide preliminary information on drugs’ ability to overcome the various biological barriers inherent in the ADME process. In this sense, in silico methods serve to provide primary information on drugs’ bioavailability mechanisms. High-performance liquid chromatography (HPLC) using a stationary phase composed of phospholipids, the so-called immobilized artificial membrane (IAM), has been widely ...
Food Bioscience, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
International Journal of Molecular Sciences, 2020
Brassicaceae vegetables are important crops consumed worldwide due to their unique flavor, and fo... more Brassicaceae vegetables are important crops consumed worldwide due to their unique flavor, and for their broadly recognized functional properties, which are directly related to their phytochemical composition. Isothiocyanates (ITC) are the most characteristic compounds, considered responsible for their pungent taste. Besides ITC, these vegetables are also rich in carotenoids, phenolics, minerals, and vitamins. Consequently, Brassica’s phytochemical profile makes them an ideal natural source for improving the nutritional quality of manufactured foods. In this sense, the inclusion of functional ingredients into food matrices are of growing interest. In the present work, Brassicaceae ingredients, functionality, and future perspectives are reviewed.
Journal of Food Composition and Analysis, 2018
Bioactive compounds of raw and cooked garlic could be absorbed at a moderate rate Raw garlic di... more Bioactive compounds of raw and cooked garlic could be absorbed at a moderate rate Raw garlic digestion showed a greater organosulfur compounds quantitative yield Garlic compound 2-vinyldithiin evidenced the best permeability and blood stability
Analytical Methods, 2018
Miniaturized extraction techniques are one of the most significant advances in analytical chemist... more Miniaturized extraction techniques are one of the most significant advances in analytical chemistry today.
Journal of Food Composition and Analysis, 2017
This review covered more than 70 studies about organosulfur compounds determination Systemati... more This review covered more than 70 studies about organosulfur compounds determination Systematic and comparative bibliographic revisions by OSCs groups were presented Alkenyl cystein sulfoxides, thiosulfinates and sulfides determinations were revised Advantages and disadvantages of different methodologies were critically discussed
Journal of Functional Foods, 2015
Numerous studies have demonstrated that garlic has many biological properties due to its phytoche... more Numerous studies have demonstrated that garlic has many biological properties due to its phytochemicals. These components include organosulphur compounds (OSCs) such as allicin, which is a chemically unstable metabolite. The aim of this study was to evaluate whether garlic could still be considered a healthy food after home cooking procedures. For that purpose, an experimental design with two factors and three levels was used. Pre-cooking and cooking procedures were the selected factors. Allicin, ajoenes, 2-vinyl-4H-1,3-dithiin (2-VD), diallyl sulphide (DAS), diallyl disulphide (DADS) and diallyl trisulphide (DATS) were the target analytes. Samples were analyzed by high performance liquid chromatography coupled to ultraviolet detector (HPLC-UV). The results showed that it was possible to find OSCs with important biological activities after all pre-cooking and cooking treatments. This is the first study to our knowledge to investigate cooked garlic using an analytical methodology, which avoid artifacts formation.
Current Computer Aided-Drug Design, 2015
The most common mechanism of the so-called multidrug resistance (MDR), is mainly associated with ... more The most common mechanism of the so-called multidrug resistance (MDR), is mainly associated with an over expression of P-glycoprotein (Pgp). It is an ATP-dependent transport protein that limits the intracellular accumulation of a variety of structurally unrelated compounds within various organs and normal tissues such as kidney, small intestine and the blood brain barrier. Thus, the expression of Pgp has a major impact on the pharmacokinetic profile of many therapeutic agents and therefore, overcoming Pgp-mediated efflux constitutes an attractive means of potentially enhancing their therapeutic efficacy. The flavonoids comprise a large group of polyphenolic compounds that occur in plants and vegetables, and they have been shown to display a wide variety of biological activities. For example, anti-inflammatory, antioxidant, antiallergic, hepatoprotective, antithrombotic, antiviral, and anticarcinogenic activities. The interactions between flavonoids and Pgp have also been extensively studied and some quantitative structure-activity relationships (QSAR) have been reported. In the present work, we have employed 2D-QSAR analysis to evaluate the interactions between Pgp and several flavonoid compounds with the aim of identifying the molecular factors responsible for the Pgp-binding affinity evidenced by these compounds. Thus, the reported data for dissociation constants (KD) between Pgp and 62 flavonoid compounds were modeled by means of multiple regression analysis (MLR), and structures of the compounds under study were characterized by means of calculated physicochemical properties and several topological and constitutional descriptors, as well as geometrical and quantum chemical indexes. The obtained results suggest that the hydrophobic and especially geometric factors are of prime importance for binding, whereas in the case of flavonoid derivatives with flavone (flavonols), flavanone and isoflavone nuclei, the electronic factors are also involved in electron donor/acceptor interactions. In addition, in the case of chalcones, the results suggest that the affinity toward P-gp of such compounds is mainly governed by intermolecular dispersive interactions at the binding site.
Journal of Food Science, 2014
Wine is a dietary source of polyphenolic compounds with reported health benefits when moderately ... more Wine is a dietary source of polyphenolic compounds with reported health benefits when moderately consumed. Several of these compounds can associate with metals forming complexes. Therefore, this work was conducted to reach a better understanding of the nature and chemical stability of wine-derived Fe 3+-quercetin complexes in a digestion model. The stability of the complexes in a synthetic (simulated) wine was studied before and after in vitro gastric and intestinal digestions by high-performance liquid chromatography (HPLC) with UV-Vis detection. Metal determination was performed by atomic absorption spectrometry (ETAAS) to evaluate possible dissociation of complexes. During HPLC analysis all peaks eluted from the chromatographic column were collected, acidified, and analyzed by ETAAS. The results showed that complexes remain substantially stable after gastric digestion conditions, with recoveries of 84% to 90%. Although metal complexes were partially degraded during intestinal digestion, 41% to 45% of the Fe 3+-quercetin complexes was recovered.
Journal of Food Composition and Analysis, 2010
... origin. Alejandra B. Camargo a , c , f , Corresponding Author Contact Information , E-mail Th... more ... origin. Alejandra B. Camargo a , c , f , Corresponding Author Contact Information , E-mail The Corresponding Author , E-mail The Corresponding Author , Sara Resnizky b , Eduardo J. Marchevsky c , d , f and Juan M. Luco e. a ...
Journal of Food Biochemistry, 2012
The aim of the present work was to evaluate the application of a physiological indicator as marke... more The aim of the present work was to evaluate the application of a physiological indicator as marker of allicin content during postharvest storage at different temperatures. Sureño INTA garlic cultivar was stored at room temperature (20C Ϯ 3) and cold storage (0C Ϯ 0.5). Samples were taken periodically and allicin, pyruvate content were analyzed and the sprout growth was measured through of the visual index of dormancy (VID). The results showed that regardless of storage temperature, allicin, pyruvate content and VID changed significantly during the period of the assay. For the conditions evaluated, the highest allicin content was observed at 50% and 100% VID. These facts suggest that VID may be a suitable tool to estimate allicin level during storage. PRACTICAL APLICATIONS This work was addressed to resolve the problem of standardization of garlic subproducts according to their bioactive compounds levels. The information acquired is important for farmers and the food and phytotherapic industry in order to guarantee the adequate supply of garlic subproducts to the consumer. Therefore, we found that it is important to consider a physiological indicator as a tool to estimate the bioactive compound levels during postharvest storage.
Journal of Chromatography A, 2011
Dispersive solid-phase extraction (DSPE) is proposed for the first time as a simplified, fast and... more Dispersive solid-phase extraction (DSPE) is proposed for the first time as a simplified, fast and low cost clean-up technique of biological sample extracts for polybrominated diphenyl ethers (PBDEs) determination. The combination of a traditional extraction technique, such as ultrasound-assisted leaching (USAL) with DSPE was successfully applied for sample preparation prior to gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis. The analytes were first extracted from 1 g homogenized sample in n-hexane:dichloromethane (8:2) by applying USAL technique and further cleaned-up using DSPE with 0.20 g C 18-silica as sorbent material. Different solvent mixtures, sorbent type and amount, and lipid digestion procedures were evaluated in terms of clean-up and extraction efficiency. Under optimum conditions, the method detection limits (MDLs) for PBDEs, calculated as three times the signal-to-noise ratio (S/N) were within the range 9-44 pg g −1 wet weight. The calibration graphs were linear within the concentration range of 53-500,000 pg g −1 , 66-500,000 pg g −1 , 89-500,000 pg g −1 and 151-500,000 pg g −1 for BDE-47, BDE-100, BDE-99 and BDE-153, respectively; and the coefficient of determination (r 2) exceeded 0.9992 for all analytes. The proposed methodology was compared with a reference solid-phase extraction technique. The applicability of the methodology for the screening of PBDEs has been demonstrated by analyzing spiked and real samples of biological nature (fish, egg and chicken) with different lipid content as well as reference material (WELL-WMF-01). Recovery values ranged between 75% and 114% and the measured concentrations in certified material showed a reasonable agreement with the certified ones. BDE-47, BDE-100 and BDE-99 were quantified in three of the seven analyzed samples and the concentrations ranged between 91 and 140 pg g −1. In addition, this work is the first description of PBDEs detected in fish of Argentinean environment.
Journal of Chromatography A, 2010
Coacervative microextraction ultrasound-assisted back-extraction technique (CME-UABE) is proposed... more Coacervative microextraction ultrasound-assisted back-extraction technique (CME-UABE) is proposed for the first time for extracting and preconcentrating organophosphates pesticides (OPPs) from honey samples prior to gas chromatography-mass spectrometry (GC-MS) analysis. The extraction/preconcentration technique is supported on the micellar organized medium based on non-ionic surfactant. To enable coupling the proposed technique with GC, it was required to back extract the analytes into hexane. Several variables including, surfactant type and concentration, equilibration temperature and time, matrix modifiers, pH and buffers nature were studied and optimized over the relative response of the analytes. The best working conditions were as follows: an aliquot of 10 mL 50 g L −1 honey blend solution was conditioned by adding 100 L 0.1 mol L −1 hydrochloric acid (pH 2) and finally extracted with 100 L Triton X-114 100 g L −1 at 85 • C for 5 min using CME technique. Under optimal experimental conditions, the enrichment factor (EF) was 167 and limits of detection (LODs), calculated as three times the signal-to-noise ratio (S/N = 3), ranged between 0.03 and 0.47 ng g −1. The method precision was evaluated over five replicates at 1 ng g −1 with RSDs ≤9.5%. The calibration graphs were linear within the concentration range of 0.3-1000 ng g −1 for chlorpirifos; and 1-1000 ng g −1 for fenitrothion, parathion and methidathion, respectively. The coefficients of correlation were ≥0.9992. Validation of the methodology was performed by standard addition method at two concentration levels (2 and 20 ng g −1). The recoveries were ≥90%, indicating satisfactory robustness of the methodology, which could be successfully applied for determination of OPPs in honey samples of different Argentinean regions. Two of the analyzed samples showed levels of methidathion ranged between 1.2 and 2.3 ng g −1 .
Journal of Agricultural and Food Chemistry, 2007
In this study, multiple linear regression (MLR) and partial least-squares (PLS) techniques were u... more In this study, multiple linear regression (MLR) and partial least-squares (PLS) techniques were used for modeling the soybean 15-lipoxygenase inhibitory activity of a varied group of mono-, di-, and trisulfides derived from the essential oil of garlic. The structures of the compounds under study were characterized by means of calculated physicochemical parameters and several nonempirical descriptors, such as topological, geometrical, and quantum chemical indices. The results obtained indicate that the inhibitory activity is strongly dependent on the ability of the compounds to participate in dispersive interactions with the enzyme, as expressed by the solvent-accessible surface area (SASA) and the average distance/distance degree descriptor (ADDD) index. On the other hand, the high contribution of the lowest unoccupied molecular orbit term (LUMO) in the PLS models derived for the di- and trisulfides suggests that the solute's electron-acceptor capacity plays a fundamental role in the inhibitory activity exhibited for these compounds. Finally, the geometric features as expressed by the shape parameters included in the models indicate a low but not negligible positive contribution of molecular linearity in the enzyme-inhibitor binding. In summary, the developed quantitative structure-activity relationship approach successfully accounts for the potencies of organosulfur compounds acting on soybean 15-lipoxygenase and thereby offers both a guide for the synthesis of new compounds and a hypothesis for the molecular basis of their activity.
Journal of Agricultural and Food Chemistry, 2007
The raw form of garlic and some of its preparations are widely recognized as antiplatelet agents ... more The raw form of garlic and some of its preparations are widely recognized as antiplatelet agents that may contribute to the prevention of cardiovascular disease. Herein, we examined the in-vitro antiaggregatory activity (IVAA) of human blood platelets induced by extracts of garlic samples that were previously heated (in the form of crushed versus uncrushed cloves) using different cooking methods and intensities. The concentrations of allicin and pyruvate, two predictors of antiplatelet strength, were also monitored. Oven-heating at 200°C or immersing in boiling water for 3 min or less did not affect the ability of garlic to inhibit platelet aggregation (as compared to raw garlic), whereas heating for 6 min completely suppressed IVAA in uncrushed, but not in previously crushed, samples. The latter samples had reduced, yet significant, antiplatelet activity. Prolonged incubation (more than 10 min) at these temperatures completely suppressed IVAA. Microwaved garlic had no effect on platelet aggregation. However, increasing the concentration of garlic juice in the aggregation reaction had a positive IVAA dose response in crushed, but not in uncrushed, microwaved samples. The addition of raw garlic juice to microwaved uncrushed garlic restored a full complement of antiplatelet activity that was completely lost without the garlic addition. Garlic-induced IVAA was always associated with allicin and pyruvate levels. Our results suggest that (1) allicin and thiosulfinates are responsible for the IVAA response, (2) crushing garlic before moderate cooking can reduce the loss of activity, and (3) the partial loss of antithrombotic effect in crushed-cooked garlic may be compensated by increasing the amount consumed.
Food Chemistry, 2014
This work proposes the novel application of a microextraction technique, solid phase microextract... more This work proposes the novel application of a microextraction technique, solid phase microextraction (SPME), coupled to liquid chromatography with UV detection (HPLC-UV) for the analysis of organosulfur compounds (OSCs) in garlic samples. Additionally, a comparative study of OSCs profiles obtained by SPME coupled to HPLC-UV and gas chromatography with flame photometric detector (GC-FPD), respectively; was carried out. This study provided complementary evidence about OSCs's lability and ''artifacts'' formation during the analytical process. Raw, cooked and distilled garlic samples were considered. The target analytes were diallyl disulphide (DADS), diallyl sulphide (DAS), diallyl trisulphide (DATS), allicin, 3-vinyl-4H-1,3-dithiin (3-VD), 2-vinyl-4H-1,2-dithiin (2-VD) and (E)-and (Z)-ajoene, which are the most important OSCs with biological activities present in raw and processed garlic. The coupling of SPME and HPLC showed to be reliable, fast, sensible and selective methodology for OSCs analysis.
European Journal of Plant Pathology, 2005
... P. hirsutum. They found that all the garlic clones were highly and equally susceptible. The i... more ... P. hirsutum. They found that all the garlic clones were highly and equally susceptible. The identification and utilization of resistant or toler-ant garlic accessions can help the management of blue mold of garlic. In the present ...
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Papers by Alejandra Vargas Camargo