Papers by Ekrem Musa ÖZDEMİR
Clinical and Experimental Health Sciences, 2021
Objective: Treatment of inflammatory bowel disease (IBD) usually involves medical therapy. For th... more Objective: Treatment of inflammatory bowel disease (IBD) usually involves medical therapy. For this reason, it is recommended to seek alternative treatment methods such as nutritional therapy. The aim of the study is to evaluate the effects of probiotics and omega-3 fatty acids on total oxidant and total antioxidant levels in an experimental colitis model. Methods: Mice were randomly divided into five groups (n= 10/group) as healthy group, colitis group, group treated with probiotics (VSL#3), group treated with omega-3 (w-3), and group treated with both probiotics and omega-3. To induce experimental colitis, 200 mg/kg dinitrobenzene sulfonic acid (DNBS) + 30% ethanol combination was rectally administered to anesthetized mice. Total oxidant (TOS) and total antioxidant (TAS) levels were measured at the tissue level. Results: Lower concentrations of TOS were observed in the probiotics groups (2.11±0.23 mmol H2O2 Eq/L), probiotics+omega-3 (2.56±1.18 mmol H2O2 Eq/L), and omega-3 (3.02±1.88 mmol H2O2 Eq/L) groups compared to the colitis group (3.11±0.91 mmol H2O2 Eq/L) (p>0.05). Higher TOS and TAS level were observed in the control colitis group compared to other groups; however, the differences were not statistically significant. Conclusion: Our findings showed that TAS and TOS levels were positively affected by the use of probiotic supplements in IBD. It was determined that using w-3 alone was ineffective in decreasing TOS levels. Studies with higher dosages and longer treatment periods are needed to better observe the effects of nutritional supplements on TOS and TAS parameters in inflammatory bowel diseases.
Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi, 2021
This study aimed to evaluate the possible neuroprotective effect of coconut oil in mice with trau... more This study aimed to evaluate the possible neuroprotective effect of coconut oil in mice with traumatic brain injury. Material and Methods:Ten adult male mice (C57BL/6) were randomly assigned into the control (n=5) and coconut oil administered group (n=5). The control group was received 250 μl saline/day and coconut oil was administered 10 mg/kg/day per os to the coconut oil group for a week. Brain coold injury model was produced on seventh day. 24 hours after the injury, infarction and oedema volume in the brain was measured in two groups. Results: The coconut oil group mice were found to exhibit lower brain infarct volume and edema than the control group mice. Conclusion: Our findings indicated that coconut oil has a potential neuroprotective effect on damaged neurons after traumatic brain injury in mice.
Korean Journal of Veterinary Research, 2021
The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkabl... more The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential has been attributed to its life-long neoteny, characterized by the exhibition of embryonic characteristics at the adult stage. A recent study provided a detailed morphological analysis of the sperm morphology of the Ambystoma mexicanum using routine and detailed histological techniques. The primary purpose of the present study is to describe a simple and inexpensive method for evaluating the morphology of axolotl sperm. In this study, spermatophore structures were collected and spread on slides and air-dried. The slides were stained with periodic acid Schiff, toluidine blue, Masson’s trichrome, Giemsa, Spermac, and Diff-Quik dye for a morphological examination. The slides were coated with gold/palladium for a scanning electron microscopy examination. The sperm of the axolotl consisted of an elongated head, a neck, and a flagellum covered with an undulating membrane. The lengt...
Medeniyet Medical Journal, 2020
In this study, we examined the effects of Polypodium vulgare L. (Polypodiaceae) as a candidate to... more In this study, we examined the effects of Polypodium vulgare L. (Polypodiaceae) as a candidate to be used for wound healing scarred area. We investigated the antibacterial, and antioxidant activity of P. Vulgare on both in vivo, and in vitro wound healing using an excisional wound model in mice Method: We used 32 Balb-c mice equally divided into four groups: Group 1 control, Group 2 vehicle, Group 3 Polypodium vulgare, and Group 4 Centella asiatica extract (CAE). All treatments were applied topically once in a day. The scar area, percentage wound closure and epithelization time were measured. PDGF, VEGF, and collagen immunohistochemical staining were used for evaluation. Results: CAE and P. vulgare extract groups were observed to be more effective than the control and vehicle groups in terms of new vascular, epidermal and granulation tissue organization. PDGF, VEGF, and collagen immunohistochemical staining was stronger in the P.vulgare extract and CAE groups compared to the control and vehicle groups. In the P. vulgare and CAE groups, PDGF staining intensity was stronger than the control and vehicle groups, but VEGF and collagen staining in P. vulgare group was not different from the control group. Conclusion: P. vulgare had an effect on the injured area by regenerating the epidermis and increasing vascularization. P. vulgare extract with known antioxidant, and antimicrobial activities may be helpful as a supportive treatment in wound healing.
Ankara Universitesi Eczacilik Fakultesi Dergisi, 2020
Objective: The aim of this study was to investigate the effects of naringin, a known flavonoid, o... more Objective: The aim of this study was to investigate the effects of naringin, a known flavonoid, on in vitro cytotoxicity, irritation and in vivo potential efficacy when topically applied to the diabetic wound. Material and Method: In vitro direct contact assay and hen's egg chorio-allantoin membrane tests were used to evaluate irritation, and cytotoxicity potential of Naringin. In vitro antimicrobial activity was also tested. Topical treatments were administered once a day on the wound. Wound lesions were photographed and statistically analyzed. After the 10th day, histopathological parameters of tissues were assessed.
Medeniyet Medical Journal, 2017
We investigated the wound healing efficacy of the methanol extracts of Prunus spinosa L. fruits u... more We investigated the wound healing efficacy of the methanol extracts of Prunus spinosa L. fruits using an excisional cutaneous wound model in mice. We used 32 C57/Black mice equally divided into four groups. Group 1 Control, Group 2 vehicle (glycerine), Group 3 Prunus spinosa L., Group 4 Madecassol. Prunus spinosa L.and Madecassol were applied for 10 days by creating a excisional wound model on the back of each mouse in each group. At the end of the 10th day, samples from wound areas were taken for histologic follow-up, scoring with hematoxylineosin and immunohistochemical (TGF-b, COL1A1) staining. Wound healing was evaluated and scored by newly formed collagen structure, increase in TGF b and epidermal and dermal regeneration, granulation tissue thickness, angiogenesis (new vessel formation). Prunus spinosa L. increased wound healing rates by promoting granulation tissue, epidermal regeneration and angiogenesis. In addition there was a significant increase in collagen and TGF [beta] ratios in immunohistochemical staining. At the end of the 4 and 10 days after treatment, a significant increase was observed in wound contraction and re-epithelialization in both Prunus spinosa and madecassol treated groups compared to the control and vehicle groups. Our findings support the beneficial effects of Prunus spinosa for augmenting wound healing. The anti-inflammatory, antioxidant and antibacterial activities of Prunus spinosa by way of increasing collagen synthesis and decreasing the number of inflammatory cells during wound healing have suggested that it may be useful for treating skin wounds.
TURKDERM, 2019
Background and Design: The objective of this study was to investigate the wound healing activity ... more Background and Design: The objective of this study was to investigate the wound healing activity of different concentrations of liposomal trans-resveratrol formulations on incisional and excisional wounds in rats. Materials and Methods: The wound healing effect was tested by an excisional and incisional wound model. Wound closure was measured for 12 days. On the 12 th day of the study, maximal load, maximum stress, stress, and % of elongation values were evaluated in the incisional wound. In addition, angiogenesis, granulation tissue thickness, epidermal and dermal regeneration values, and macroscopic photographic analyses were evaluated in the excisional wound. Results: When the wound tissue surface healing rates were evaluated, similar effects were observed at the end of the 10 th and 12 th days between the 5% Res group and the commercial product containing 1% Centella asiatica extract used as the reference molecule. Histological evaluation showed that 1% Res and 5% Res groups induced significant wound healing activity compared to the control group. Furthermore, 1% Res and 5% Res groups increased wound healing rates by promoting granulation tissue, epidermal, and dermal regeneration as well as angiogenesis. Conclusion: Liposomal formulations containing 1% and 5% resveratrol were found to have positive effects on the healing process, both on excisional and incisional wound tissues.
Biotechnic & Histochemistry, 2018
Laurocerasus officinalis Roem. (syn: Prunus laurocerasus L.) is a member of Rosaceae family. We i... more Laurocerasus officinalis Roem. (syn: Prunus laurocerasus L.) is a member of Rosaceae family. We investigated the antimicrobial and antioxidant activity of L. officinalis Roem in wound healing both in vivo and in vitro using an excisional wound model model in mice. We used four groups of eight mice as follows: untreated (control), empty gel, extract + gel (L. officinalis + gel), and Madecassol® groups. All treatments were applied topically once daily. The scar area, percentage wound closure and epithelization time were measured. L. officinalis promoted wound healing and increased granulation tissue, epidermal regeneration and angiogenesis. L. officinalis extract, which is known for its antioxidant and antimicrobial activities, may be useful for promoting wound healing.
Journal of the Turkish German Gynecological Association, Jun 19, 2018
Besides its hematopoietic function, the erythropoietin (EPO) may protect tissues from degenerativ... more Besides its hematopoietic function, the erythropoietin (EPO) may protect tissues from degenerative disorders. As such, EPO and its receptors were revealed in nonhematopoietic cells, including stromal and endometrial epithelial- cells. However, the role of EPO in endometrial disorders was still unknown. Here we aimed to examine the role of EPO and its receptor activation in the development of endometriosis in rats. Animals were treated with EPO, darbepoietin (synthetic form of EPO) or EPO's receptor activator, Mircera, after development of endometriosis. Endometriosis was induced by estrogen-administration following surgical attachment of endometrial surface on the inner abdominal wall. Treatments were started 3 weeks after induction of endometriosis and continued for the following 3 weeks. For the analysis of recurrence of endometriosis, additional analyses were conducted 3 weeks after cessation of treatments. As compared with vehicle- treated animals, lesion size was reduced si...
Acta Medica Alanya, 2019
Amaç: "TNF-alfa inhibitörü Adalimumab'ın, anti-inflamatuvar etkisi nedeniyle inflamasyonla ilişki... more Amaç: "TNF-alfa inhibitörü Adalimumab'ın, anti-inflamatuvar etkisi nedeniyle inflamasyonla ilişkili nöronal hasarı etkileyebileceği" hipotezine dayanarak adalimumab'ın nöroprotektif rolünü araştırmayı amaçladık. Metotlar: Adalimumab'ın etkilerini araştırmak için soğuk travma modelini kullanarak farelerde beyin hasarı oluşturduk ve krezil viyole boyasıyla hücre sağ-kalım/ölüm oranları ile görüntü analiz sistemi ile hesaplanan infarktüs/ödem hacmini değerlendirdik. Bulgular: Verilerimiz infarkt ve ödem hacminde azalma eğilimi göstermesine rağmen istatistiksel olarak anlamlı değildir. Sonuç: Bildiğimiz kadarıyla çalışmamız adalimumab'ın hasarlı nöronlar üzerindeki nöroprotektif etkisini değerlendiren ilk çalışmadır.
Uploads
Papers by Ekrem Musa ÖZDEMİR