Papers by Massimo Bertamini
Atti del XLVII …, 2003
Moser, C., Pindo, M., Segala, C., Fontana, P., Blanzieri, E., Gatto, P., et al. (2003). Dissectin... more Moser, C., Pindo, M., Segala, C., Fontana, P., Blanzieri, E., Gatto, P., et al. (2003). Dissecting grape leaf development and senescence at the molecular level. In Atti del XLVII Convegno annuale della Società italiana di genetica agraria (pp. poster abstract 4.06).
Notiziario tecnico CRPV, 1998
Horticulturae
Shoot topping and other summer grapevine management practices are considered crucial for producin... more Shoot topping and other summer grapevine management practices are considered crucial for producing high-quality wine. However, in recent years, climate change is increasing the need to reassess these strategies, as excessive radiation and high temperatures can negatively impact canopy functionality and berry quality. Indeed, it has been hypothesized that limiting summer vegetative pruning may protect the bunch, via shading, and the leaf by maintaining a more favorable environment for leaf functionality (e.g., lower VPD, reduced high light stress) owing to a denser canopy. In this work, a series of canopy manipulation treatments (shoot topping vs. long-shoot bundling; secondary shoot trimming vs. untrimmed) were tested in a replicated factorial block design over two seasons in field-grown grapevine plants (cv. Cabernet Franc grafted in SO4). Overall, treatments in which secondary shoot removal and/or shoot topping were not applied produced a higher canopy area, increased pruning wood...
Horticulturae, 2022
Compact bunches have been often associated with higher susceptibility to Botrytis cinerea and the... more Compact bunches have been often associated with higher susceptibility to Botrytis cinerea and therefore reduction in berry quality in grapevine. The objective of this study was to evaluate three management methods (early leaf removal, gibberellic acid, and their combination) for reducing bunch compactness in Vitis vinifera cv. Pinot gris trained in two different training systems with contrasting vigor (Guyot and pergola). Treatments were applied at BBCH 62 or BBCH 65 and yield components, total soluble solids, fruit set, and bunch compactness parameters were evaluated. Both treatments individually reduced berry number, mean bunches weight and bunches compactness as well as yield per vine when compared to control-untreated vines. However, no major differences were observed when both the treatments were applied in combination for Guyot or pergola although a higher reduction in yield was detected for Guyot and a significant increase in total soluble solids was observed in pergola. Our ...
Journal of Experimental Botany, 2021
Stomata control CO2 uptake for photosynthesis and water loss through transpiration, thus playing ... more Stomata control CO2 uptake for photosynthesis and water loss through transpiration, thus playing a key role in leaf thermoregulation, water-use efficiency (iWUE), and plant productivity. In this work, we investigated the relationship between several leaf traits and hypothesized that stomatal behavior to fast (i.e. minutes) environmental changes co-determines, along with steady-state traits, the physiological response of grapevine to the surrounding fluctuating environment over the growing season. No relationship between iWUE, heat stress tolerance, and stomatal traits was observed in field-grown grapevine, suggesting that other physiological mechanisms are involved in determining leaf evaporative cooling capacity and the seasonal ratio of CO2 uptake (A) to stomatal conductance (gs). Indeed, cultivars that in the field had an unexpected combination of high iWUE but low sensitivity to thermal stress displayed a quick stomatal closure to light, but a sluggish closure to increased vapor...
Plant Science, 2003
Photoinhibition of photosynthesis was studied in young (fully expanded) and mature sun leaves of ... more Photoinhibition of photosynthesis was studied in young (fully expanded) and mature sun leaves of grapevine (Vitis vinifera L.), under controlled conditions (irradiation of detached leaves to about 1900 mE m (2 s (1). The degree of photoinhibition was determined by means of the ratio of variable to maximum chlorophyll (Chl) fluorescence (Fv/Fm) and electron transport measurements. Compared with the mature leaves, the young leaves, containing about half the amount of Chl a'/b per unit area, exhibited a higher proportion of total carotenoids as xanthophyll cycle pigments and had an increased ratio of total carotenoids to Chl a'/b. The potential efficiency of PS II, Fv/Fm, markedly declined in high light irradiated young leaves without significant increase of Fo level. In contrast, Fv/Fm ratio declined with significant increase of Fo level in mature leaves. When various photosynthetic activities were followed on isolated thylakoids, the rate of whole chain and PS II activity were markedly decreased in high light irradiated young leaves than mature leaves. A smaller inhibition of PS I activity was also observed in both leaves. In the subsequent dark incubation, fast recovery was observed in both leaves and reached maximum PSII efficiencies similar to those observed in non-photoinhibited leaves. The artificial exogenous electron donors DPC, NH 2 OH and Mn 2' failed to restore the high light induced loss of PS II activity in mature leaves, while DPC and NH 2 OH significantly restored in young leaves. It is concluded that high light inactivates on the donor side of PS II and acceptor side of PS II in young and mature leaves, respectively. Quantification of the PS II reaction center protein D1 and 33 kDa protein of water splitting complex following high light exposure of leaves showed pronounced differences between young and mature leaves. The marked loss of PS II activity in high light irradiated leaves was due to the marked loss of D1 protein of the PS II reaction center and 33 kDa protein of the water splitting complex in mature and young leaves, respectively.
Physiological and Molecular Plant Pathology, 2002
We have studied the eect of the apple proliferation phytoplasmal infection on some features of th... more We have studied the eect of the apple proliferation phytoplasmal infection on some features of the thylakoids from ®eld grown apple (Malus pumila) leaves. Changes in photosynthetic pigments, soluble proteins, ribulose-1,5-bisphosphate carboxylase, nitrate reductase, photosynthetic activities and thylakoid membrane proteins were investigated. The level of total chlorophyll and carotenoids were reduced in phytoplasma-infected leaves. Similar results were also observed for soluble proteins and ribulose-1,5-bisphosphate carboxylase activity. The in vivo nitrate reductase activity was signi®cantly reduced in infected leaves. When various photosynthetic activities were followed in isolated thylakoids, phytoplasmal infection caused marked inhibition of whole chain and photosystem II activity while the inhibition of photosystem I activity was only marginal. The arti®cial exogenous electron donors, diphenyl carbazide and hydroxylamine signi®cantly restored the loss of photosystem II activity in infected leaves. The same results were obtained when F v /F m was evaluated by chlorophyll¯uorescence measurements. The marked loss of photosystem II activity in infected leaves could be due to the loss of 47, 33, 28±25, 23 and 17 kDa polypeptides. It is concluded that phytoplasmal infection inactivates the donor side of photosystem II. This conclusion was con®rmed by immunological studies showing that the content of the 33 kDa protein of the water-splitting complex was diminished signi®cantly in infected leaves.
Physiological and Molecular Plant Pathology, 2005
Investigations were carried out to envisage the effect of the cypress canker infection on some fe... more Investigations were carried out to envisage the effect of the cypress canker infection on some features of the thylakoids from field grown cypress (Cupressus sempervirens) needles. Changes in photosynthetic pigments, soluble proteins, soluble starch, starch, ribulose-1,5-bisphosphate carboxylase, nitrate reductase, photosynthetic activities and thylakoid membrane proteins were investigated. The level of total chlorophyll and carotenoids were markedly reduced in cypress canker-infected needles. Similar results were also observed for soluble proteins, nitrate reductase and ribulose-1,5-bisphosphate carboxylase activity. In contrast, the content of soluble starch and sugar were increased in infected needles. In isolated thylakoids, cypress canker infection caused marked inhibition of whole chain and photosystem II activity while the inhibition of photosystem I activity was only marginal. The artificial exogenous electron donors, diphenyl carbazide and hydroxylamine significantly restored the loss of photosystem II activity in infected needles. The same results were obtained when Fv/Fm was evaluated by chlorophyll fluorescence measurements. The marked loss of photosystem II activity in infected needles could be due to the loss of 47, 43, 33, 28, 25, 23, 17 and 15 kDa polypeptides. It is concluded that cypress canker infection inactivates the donor side of photosystem II. This conclusion was confirmed by immunological studies showing that the content of the 33 kDa protein of the water-splitting complex was diminished significantly in infected needles.
Several elements of anthropogenic and natural origin can settle on the grape berry skin. In this ... more Several elements of anthropogenic and natural origin can settle on the grape berry skin. In this study, the effectiveness of 3 washing methods (using ultrapure water, 0.2% acetic acid and 0.2% citric acid) in removing contaminants was tested on laboratory scale, in comparison with an analytical washing preparation of reference with nitric acid 1%. Forty-four macro, micro and trace elements were quantified in the solutions after berry washing treatment. The analysis was carried out with an ICP-MS. The citric acid solution provided comparable or only slightly less effective results in comparison with HNO 3-wahing method, whereas acetic acid and water removed on average only 45% of the contaminants removable using the reference method. The technological removal of pollutant elements using washing solution needs further research as regards environmental sustainability because of the consumption of drinking water.
Proceedings of the …, 2005
In grapevine, such as in many other fleshy fruits, the transition from the green, hard and acidic... more In grapevine, such as in many other fleshy fruits, the transition from the green, hard and acidic berry to the sweet, soft, colored and sugar rich ripe fruit, involves biochemical and biophysical processes finely tuned at the transcriptional level. The berry development ...
Physiological and Molecular Plant Pathology, 2002
Plant Science, 2002
In this work we have studied the influence of lime-induced iron deficiency on some features of th... more In this work we have studied the influence of lime-induced iron deficiency on some features of the thylakoids and PS II membranes from grapevine (Vitis vinifera L. cv. Pinot noir) plants grown in calcareous soils. Changes in photosynthetic activities and PS II membrane polypeptides were investigated. When various photosynthetic electron transport activities were analyzed in isolated thylakoids, a major decrease in the rate of whole chain (H 2 O0/MV) electron transport was observed in chlorotic leaves. Such reduction was mainly due to the loss of PS II activity. Smaller inhibition of PS I activity was also observed in chlorotic leaves. The artificial electron donors, DPC and NH 2 OH markedly restored the loss of PS II activity in chlorotic leaves. The same results were obtained when variable fluorescence/maximum fluorescence (Fv/Fm) was evaluated by chlorophyll (Chl) fluorescence measurements. The marked loss of PS II activity in chlorotic leaves was evidently due to the loss of D1, 33, 28 Á/25, and 23 kDa polypeptides. This conclusion was confirmed by immunological studies showing that the content of the D1 protein of the PS II reaction centre and of the 33 kDa protein of the water-splitting complex was diminished significantly in chlorotic leaves.
Plant Physiology and Biochemistry, 2005
Changes of leaf pigments, ribulose-1,5-bisphosphate carboxylase (RuBPC) and photosynthetic effici... more Changes of leaf pigments, ribulose-1,5-bisphosphate carboxylase (RuBPC) and photosynthetic efficiency were examined in grapevine (Vitis vinifera L.) plants grown under ambient irradiation (maximum daily PAR = 1500 µmol m −2 s −1) for 7 days to low night temperature (LNT) of 5°C (daily from 18:00 to 06:00). The contents of chlorophyll (Chl) and carotenoids (Car) per fresh mass were lower in LNT leaves than in control leaves. The contents of a + b carotene and lutein-5,6-epoxide remained unaffected, but the de-epoxidation state involving the components of xanthophyll cycle increased. RuBPC activity and soluble proteins were also significantly reduced in LNT leaves. In isolated thylakoids, a marked inhibition of whole chain (PS I + PS II) and PS II activity were observed in LNT leaves. Smaller inhibition of PS I activity was observed in LNT leaves. The artificial exogenous electron donors, MnCl 2 , DPC and NH 2 OH did not restored the loss of PS II activity in LNT leaves. The same results were obtained when F v /F m was evaluated by Chl fluorescence measurements. The marked loss of PS II activity in LNT leaves was due to the marked loss of D1 protein which was determined by immunological studies.
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Papers by Massimo Bertamini