Papers by Alexander Morley
Blood, 1971
Serum obtained from mice after irradiation shows an enhanced ability to stimulate growth of granu... more Serum obtained from mice after irradiation shows an enhanced ability to stimulate growth of granulocytic colonies in vitro. This enhanced stimulatory ability of serum is related in both time and magnitude to the neutropenia developing after irradiation and in time to the granulopoietic response of the marrow to the neutropenia. The relationship suggests that the active factor in postirradiation serum might be granulopoietin, the physiological regulator of granulopoiesis.
New England Journal of Medicine, 1998
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Australasian Annals of Medicine, 1969
Two patients with polycyth.emia vera were studied in order to determine whether blood-cell cycles... more Two patients with polycyth.emia vera were studied in order to determine whether blood-cell cycles were present. Neutrophil and platelet cycles were found in one and a reticulocyte cycle in the other. The findings suggest that control of cell production by the marrow is partly retained in polycyth<emia vera. In contrast to the findings in chronic granulocytic leuk<emia, no evidence was found for slowed cellular maturation.
Experimental Biology and Medicine, 1971
... (ADP, Sigma), adenosine triphosphate (ATP, Sigma), insulin (Lilly) and nicotinic acid (Lilly)... more ... (ADP, Sigma), adenosine triphosphate (ATP, Sigma), insulin (Lilly) and nicotinic acid (Lilly) . ... An inhibitory effect on colony formation was produced by cAMP in each of 4 experiments, by dibutyrylcAMP in each of 2 and by epinephrine and aminophylline in each of 3. For any one ...
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1999
There is abundant evidence of the potential for exogenous agents to cause cancer but the proporti... more There is abundant evidence of the potential for exogenous agents to cause cancer but the proportion of human cancers attributable to defined external agents is uncertain. With rare exceptions it is difficult to demonstrate a role for exogenous agents in increasing mutation above background rates. There are many sources of endogenous mutation including physico-chemical processes, free radicals and enzymatic processes controlling DNA damage and repair. Evidence for the role of diet and genetic factors as major determinants of endogenous mutagenesis is reviewed with reference to the spontaneous spectrum of mutations in human cells and the quantitative measurement of mutation frequency in dietary restriction and the senescence-accelerated mouse.
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1988
Mutation Research Letters, 1983
The autoradiographic detection of thioguanine-resistant (TGR) cells has been proposed as a method... more The autoradiographic detection of thioguanine-resistant (TGR) cells has been proposed as a method for measurement of in vivo mutation frequency. This proposition was tested by combining the autoradiographic technique with the technique of long-term lymphocyte culture. Irradiation of lymphocytes on day 0 following by culture showed a dose-related increase the frequency of TGR cells with an expression time of approx. 7 days. There was a suggestion that mis-sense mutations as well as non-sense mutations and deletions were increased. Incubation with aminopterin for 10 days prevented the increase in TGR cells after irradiation and probably decreased the frequency of TGR cells prior to irradiation. The results suggest that many or most of the TGR cells measured autoradiographically are mutant cells. However, the variability observed with the method imposes limitations on its use and suggests that it will be of more value in studying populations rather than individuals.
Cancer Genetics and Cytogenetics, 1986
A cell line (RCH-ACV) was established from a bone marrow sample of a child with acute lymphablast... more A cell line (RCH-ACV) was established from a bone marrow sample of a child with acute lymphablastic leukemia (ALL). The cell line lacked Epstein-Barr virus nuclear antigen and exhibited a recently described nonrandom chromosome translocation, 1;19, thought to be associated with pre-BALL and poor prognosis. Banding studies confirm that the breakpoint of chromosome #19 occurs at p13.3. Cell surface marker analysis using a panel of monoclonal antibodies revealed markers consistent with common ALL phenotype. Although the cells did not show cytoplasmic immunoglobulin, studies of the immunoglobulin gene rearrangement confirmed the pre-B phenotype. This cell line could be of great value to studies of the role of the specific translocation 1;19 in the etiology of pre-BALL .
Experientia, 1979
The sensitivity of human peripheral blood lymphocytes to bleomycin and mitomycin-C was assessed b... more The sensitivity of human peripheral blood lymphocytes to bleomycin and mitomycin-C was assessed by measuring the inhibition of phytohemagglutinin stimulated proliferation. The sensitivity to bleomycin, and not to mitomycin-C, increased with the age.
British Journal of Haematology, Oct 1, 1970
We have studied the pattern ofhaemopoietic recovery in mice irradiated whilst one leg was shielde... more We have studied the pattern ofhaemopoietic recovery in mice irradiated whilst one leg was shielded. In the shielded limb the number of colony forming units remained approximately constant, the number of lymphocytes decreased almost immediately and then slowly rose, and granulopoietic hyperplasia developed in response to the neutropenia produced by irradiation. Shortly after the development of granulopoietic hyperplasia, the number of erythroid precursors declined despite a fall in haematocrit. This reciprocal relationship between granulopoiesis and erythropoiesis was probably due to stem cell competition, since polycythaemic mice, in which erythropoiesis was suppressed, showed a significantly greater granulopoietic response to neutropenia. In the irradiated limb the number of colony forming units increased exponentially between days 4 and 12 and more slowly thereafter. Erythropoiesis was the first differentiated cell series to recover, doing so with such rapidity that during days 8-12 the number of erythroid precursors at least equalled and the "Fe uptake in the irradiated limb actually exceeded that in the shielded limb. Several explanations may be advanced for the dichotomy between the shielded limb, in which granulopoiesis predominated, and the irradiated limb, in which erythropoiesis predominated, but the most likely explanation is that normal unirradiated marrow responds P 3
endo-toxin intoCF,mice leads toelevatedserum colony-stimulating factor (CSF)levels along with atr... more endo-toxin intoCF,mice leads toelevatedserum colony-stimulating factor (CSF)levels along with atransient granulo-cytopenia followed bytherelease ofgranulocytes from the marrow. Thepresentstudydemonstratesthatthenumber oftibial CFC, asassayed onsoft agar, decreases within 20mmafter endotoxin, reaches anadir at6hr
Blood, 1999
To the Editor: In a recent issue of Blood, Haurie et al[1][1] provided a detailed and historical ... more To the Editor: In a recent issue of Blood, Haurie et al[1][1] provided a detailed and historical review of cyclical neutropenia and other periodic phenomena in hematopoiesis. There are several points related to the review that need emphasis. The feedback hypothesis as originally proposed
Blood, 1974
An animal model of chronic aplastic marrow failure was produced by administering busulfan to mice... more An animal model of chronic aplastic marrow failure was produced by administering busulfan to mice in high dosage for a prolonged period. Mice which survived 60 or more days following cessation of the drug appeared well: in 59% the blood was normal, and in 35% the marrow cellularity was within the normal range; those mice with evident abnormality in the blood and marrow showed only minimal cytopenia in the peripheral blood and moderate decreases in marrow cellularity. Nevertheless, by 240 days 80% of the mice had become ill and died. Sacrifice prior to death showed that aplasia of the marrow and cytopenia (most often pancytopenia) were present in the majority of mice. These syndromes of persistent mild marrow hypoplasia and late severe marrow failure which followed busulfan may be suitable experimental models for studying the late effects of myelotoxic agents and the syndrome of chronic aplastic marrow failure in man.
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Papers by Alexander Morley